CaMPDB :: Substrate

XSB1253 : calpain 1, large subunit [Bos taurus]

This entry is computationally expanded from SB0020

* Basic Information

Organism	Bos taurus (cattle)
Protein Names	Calpain-1 catalytic subunit; 3.4.22.52; Calpain-1 large subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; muCANP; Micromolar-calpain; calpain 1; large subunit; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; mu-calpain
Gene Names	CAPN1; calpain 1, (mu/I) large subunit
Gene Locus	29; chromosome 29
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_776684	NM_174259	281661	Q27970	N/A	N/A	N/A	bta:281661

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 716 aa

Average Mass: 82.207 kDa

Monoisotopic Mass: 82.155 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 15 ---
Peptidase_C2 1.	55	354	796.0	2.4e-236
Calpain_III 1.	365	524	378.2	1.5e-110
efhand 1.	548	575	0.1	20
efhand 2.	591	619	16.6	0.11
efhand 3.	621	649	15.4	0.23
efhand 4.	686	713	6.2	4.5

3. Sequence Information

Fasta Sequence: XSB1253.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] ITPVYCTGVS¹⁵-AQVQKQRAKE

Ser¹⁵ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile⁶	Thr⁷	Pro⁸	Val⁹	Tyr¹⁰	Cys¹¹	Thr¹²	Gly¹³	Val¹⁴	Ser¹⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala¹⁶	Gln¹⁷	Val¹⁸	Gln¹⁹	Lys²⁰	Gln²¹	Arg²²	Ala²³	Lys²⁴	Glu²⁵

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAEEFITPVYCTGVSAQVQKQRAKELGLGRHENAIKYLGQDYEQL

Summary

#	organism	max score	hits	top seq
1	Bos taurus	94.40	6	micromolar calcium activated neutral protease 1
2	Macaca mulatta	92.00	3	PREDICTED: calpain 1, large subunit isoform 4
3	Homo sapiens	89.70	21	CAPN1 protein
4	Pan troglodytes	89.70	5	PREDICTED: hypothetical protein isoform 9
5	Canis familiaris	89.70	4	PREDICTED: similar to Calpain-1 catalytic subunit
6	synthetic construct	89.70	1	Homo sapiens calpain 1, (mu/I) large subunit
7	Pongo pygmaeus	89.70	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
8	N/A	89.00	11	C
9	Sus scrofa	89.00	4	micromolar calcium-activated neutral protease 1 is
10	Rattus norvegicus	87.80	5	calpain 1
11	Mus musculus	84.30	15	unnamed protein product
12	Xenopus laevis	72.40	7	MGC81785 protein
13	Xenopus tropicalis	71.60	3	CAPN1 protein
14	Gallus gallus	70.50	5	mu-calpain large subunit
15	Danio rerio	45.40	16	AF282675_1 calpain 1
16	Coturnix coturnix	43.50	1	quail calpain
17	Monodelphis domestica	42.40	1	PREDICTED: similar to calpain 2
18	catalytic	42.00	1	Calpain 2, large
19	Stizostedion vitreum vitreum	40.80	1	putative calpain-like protein
20	Oncorhynchus mykiss	39.30	3	calpain 1 catalytic subunit
21	Tetraodon nigroviridis	37.40	2	unnamed protein product

Top-ranked sequences

organism	matching
Bos taurus	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45
Macaca mulatta	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAEEIITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45
Homo sapiens	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Pan troglodytes	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Canis familiaris	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| Sbjct 1 MAEEIITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYMGQDFEQL 45
synthetic construct	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
Pongo pygmaeus	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|+\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSEEIITPVYCTGVS#AQVQKQRARELGLGRHENAIKYLGQDYEQL 45
N/A	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45
Sus scrofa	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAEEVITPVYCTGVS#AQVQKLRAKELGLGRHENAIKYLGQDYEQL 45
Rattus norvegicus	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 1 MAEELITPVYCTGVS#AQVQKQRDKELGLGRHENAIKYLGQDYENL 45
Mus musculus	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \| \|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 1 MTEELITPVYCTGVS#AQVQKKRDKELGLGRHENAIKYLGQDYETL 45
Xenopus laevis	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \| \|\|\| \|\|+\|#\|\|+\|\|\|\| \|\|\|+\|+\|\|\|\|+\|+\|\|\|\|\|+++ Sbjct 1 MAEEVIVPVYATGIS#AQLQKQRDHELGVGKHENAVKFLGQDYDRI 45
Xenopus tropicalis	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| \| \|\|\| +\|+\|#\|\|+\|\|\|\| +\|\|\|+\|+\|\|\|\|+\|+\|\|\|\|\|+++ Sbjct 1 MAEEVIVPVYASGIS#AQLQKQRDQELGVGKHENAVKFLGQDYDRI 45
Gallus gallus	Query 1 MAEEFITPVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\|\|\| + \|\|+\|\|\|\|\|#\|\|\|\|+\|\|\|\| \|\|\|\|+\|+\|\|+++ \|\|\|\| \| Sbjct 1 MAEEPVVPVFCTGVS#AQVQRQRAKALGLGQHQNAVRFRGQDYAAL 45
Danio rerio	Query 8 PVYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|+ \|\|++#\|+++ \| ++ \|\|\|++ \|\|+\|+\|\|\|\|\|\| \| Sbjct 3 PICATGMA#ARLRSQWDRDAGLGQNHNAVKFLGQDYETL 40
Coturnix coturnix	Query 13 GVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|++#\|++\|+ \| + \|+\| \| \|\|+\|\|\| \|\|\|\| \| Sbjct 6 GIA#ARLQRDRLRAEGVGEHNNAVKYLNQDYEAL 38
Monodelphis domestica	Query 12 TGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 +\|++#\|+\| + \| \|\|\| \|\| \|+\|\|\| \|\|\|\| \| Sbjct 2 SGIA#AKVVRDREAAAGLGTHEQAVKYLNQDYETL 35
catalytic	Query 13 GVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|++#\|++ \| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \| Sbjct 32 GIA#AKLAKDREAAEGLGSHERAIKYLNQDYEAL 64
Stizostedion vitreum vitreum	Query 12 TGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 +\|++#\| +\|++\| \|\| \|+\| \|+\|\|\| \|\|\|\| \| Sbjct 2 SGIA#ATLQRRREKEQGIGSCSQAVKYLNQDYEAL 35
Oncorhynchus mykiss	Query 9 VYCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 \|\| +\|++#\|+++ \| ++ \|\|\|++ \|\|+\|+ \|\|\|++ \| Sbjct 4 VYASGMA#AKLRSQWDRDEGLGQNHNAVKFQGQDFKSL 40
Tetraodon nigroviridis	Query 10 YCTGVS#AQVQKQRAKELGLGRHENAIKYLGQDYEQL 45 + \|\|++# +++ \| ++ \|\|\|++ \|+\|+\|\|\|\|\|\| \| Sbjct 7 HATGMA#YKLRSQWDRDEGLGQNHKAVKFLGQDYELL 42

* References

[PubMed ID: 18682401] Averna M, Stifanese R, De Tullio R, Passalacqua M, Salamino F, Pontremoli S, Melloni E, Functional role of HSP90 complexes with endothelial nitric-oxide synthase (eNOS) and calpain on nitric oxide generation in endothelial cells. J Biol Chem. 2008 Oct 24;283(43):29069-76. Epub 2008 Aug 5.

» More (9) « Hide Below

[PubMed ID: 18423040] ... Cheong HS, Yoon DH, Park BL, Kim LH, Bae JS, Namgoong S, Lee HW, Han CS, Kim JO, Cheong IC, Shin HD, A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle. BMC Genet. 2008 Apr 19;9:33.

[PubMed ID: 17868638] ... Kar P, Chakraborti T, Roy S, Choudhury R, Chakraborti S, Identification of calpastatin and mu-calpain and studies of their association in pulmonary smooth muscle mitochondria. Arch Biochem Biophys. 2007 Oct 15;466(2):290-9. Epub 2007 Aug 7.

[PubMed ID: 17603104] ... Barendse W, Harrison BE, Hawken RJ, Ferguson DM, Thompson JM, Thomas MB, Bunch RJ, Epistasis between calpain 1 and its inhibitor calpastatin within breeds of cattle. Genetics. 2007 Aug;176(4):2601-10. Epub 2007 Jul 1.

[PubMed ID: 16297652] ... Raynaud F, Jond-Necand C, Marcilhac A, Furst D, Benyamin Y, Calpain 1-gamma filamin interaction in muscle cells: a possible in situ regulation by PKC-alpha. Int J Biochem Cell Biol. 2006 Mar;38(3):404-13. Epub 2005 Oct 27.

[PubMed ID: 15523157] ... Juszczuk-Kubiak E, Sakowski T, Flisikowski K, Wicinska K, Oprzadek J, Rosochacki SJ, Bovine mu-calpain (CAPN1) gene: new SNP within intron 14. J Appl Genet. 2004;45(4):457-60.

[PubMed ID: 14622253] ... Raynaud F, Bonnal C, Fernandez E, Bremaud L, Cerutti M, Lebart MC, Roustan C, Ouali A, Benyamin Y, The calpain 1-alpha-actinin interaction. Resting complex between the calcium-dependent protease and its target in cytoskeleton. Eur J Biochem. 2003 Dec;270(23):4662-70.

[PubMed ID: 12016516] ... Heaton MP, Harhay GP, Bennett GL, Stone RT, Grosse WM, Casas E, Keele JW, Smith TP, Chitko-McKown CG, Laegreid WW, Selection and use of SNP markers for animal identification and paternity analysis in U.S. beef cattle. Mamm Genome. 2002 May;13(5):272-81.

[PubMed ID: 11048924] ... Smith TP, Casas E, Rexroad CE 3rd, Kappes SM, Keele JW, Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness. J Anim Sci. 2000 Oct;78(10):2589-94.

[PubMed ID: 2065086] ... Zimmerman UJ, Schlaepfer WW, Two-stage autolysis of the catalytic subunit initiates activation of calpain I. Biochim Biophys Acta. 1991 Jun 24;1078(2):192-8.