XSB1592 : major intrinsic protein of eye lens fiber [Mus musculus]
[ CaMP Format ]
This entry is computationally expanded from SB0003
* Basic Information
| Organism | Mus musculus (house mouse) |
| Protein Names | Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26; major intrinsic protein of eye lens fiber; hydropic fibers; lens opacity; aquaporin 0; dominant cataract |
| Gene Names | Mip; Palm; major intrinsic protein of eye lens fiber |
| Gene Locus | 10 74.0 cM; chromosome 10 |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 263 aa
Average Mass: 28.193 kDa
Monoisotopic Mass: 28.175 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| MIP 1. | 3 | 219 | 418.9 | 8.4e-123 |
3. Sequence Information
Fasta Sequence: XSB1592.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
1 [sites]
Cleavage sites (±10aa)
[Site 1] SVSERLSILK238-GARPSDSNGQ
Lys238 
Gly
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ser229 | Val230 | Ser231 | Glu232 | Arg233 | Leu234 | Ser235 | Ile236 | Leu237 | Lys238 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gly239 | Ala240 | Arg241 | Pro242 | Ser243 | Asp244 | Ser245 | Asn246 | Gly247 | Gln248 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| IIGGGLGSLLYDFLLFPRLKSVSERLSILKGARPSDSNGQPEGTGEPVELKTQAL |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 110.00 | 14 | major intrinsic protein of eye lens fiber |
| 2 | Rattus norvegicus | 110.00 | 7 | PREDICTED: similar to major intrinsic protein of e |
| 3 | N/A | 110.00 | 5 | - |
| 4 | Canis lupus familiaris | 110.00 | 2 | major intrinsic protein of lens fiber |
| 5 | Rattus rattus | 110.00 | 2 | MIP_RAT Lens fiber major intrinsic protein (Aquapo |
| 6 | Homo sapiens | 108.00 | 16 | putative alternative lens membrane intrinsic prote |
| 7 | Oryctolagus cuniculus | 108.00 | 1 | aquaporin |
| 8 | Cavia porcellus | 107.00 | 1 | MIP_CAVPO Lens fiber major intrinsic protein (Aqua |
| 9 | Bos taurus | 101.00 | 4 | major intrinsic protein of lens fiber |
| 10 | Macaca mulatta | 97.80 | 4 | PREDICTED: major intrinsic protein of lens fiber |
| 11 | Monodelphis domestica | 95.10 | 2 | PREDICTED: similar to lens-specific aquaporin-0; M |
| 12 | Rana pipiens | 86.30 | 1 | CAA40291.1 |
| 13 | Tetraodon nigroviridis | 79.30 | 3 | unnamed protein product |
| 14 | Xenopus laevis | 76.30 | 4 | hypothetical protein LOC495140 |
| 15 | Xenopus tropicalis | 76.30 | 2 | hypothetical protein LOC100037914 |
| 16 | Danio rerio | 73.90 | 6 | major intrinsic protein of lens fiber 1 |
| 17 | Fundulus heteroclitus | 71.60 | 1 | AF191906_1 lens major intrinsic protein |
| 18 | Gallus gallus | 51.20 | 5 | major intrinsic protein of lens fiber |
| 19 | Pan troglodytes | 46.20 | 7 | PREDICTED: aquaporin 2 isoform 3 |
| 20 | synthetic construct | 46.20 | 3 | aquaporin 2 |
| 21 | human, kidney, Peptide, 271 aa | 46.20 | 1 | hAQP-CD=collecting duct aquaporin |
| 22 | Notomys alexis | 45.40 | 1 | aquaporin 2 |
| 23 | Canis familiaris | 45.10 | 4 | PREDICTED: similar to aquaporin 2 |
| 24 | Ovis aries | 42.70 | 2 | AQP2_SHEEP Aquaporin-2 (AQP-2) (Aquaporin-CD) (AQP |
| 25 | Bufo marinus | 42.40 | 2 | water channel homolog |
| 26 | Coturnix coturnix | 41.20 | 2 | aquaporin-2 |
| 27 | Hyla chrysoscelis | 38.10 | 2 | aquaporin 2-like |
| 28 | Equus caballus | 37.40 | 1 | aquaporin 5 |
| 29 | Aeromonas hydrophila subsp. hydrophila ATCC 7966 | 35.80 | 1 | dienelactone hydrolase domain protein |
| 30 | Passer domesticus | 35.40 | 1 | aquaporin-1 |
| 31 | Hyla japonica | 33.90 | 2 | water channel protein AQP-h2 |
| 32 | Sus scrofa | 33.50 | 1 | aquaporin 1 |
| 33 | Pongo pygmaeus | 33.10 | 1 | AQP1_PONPY Aquaporin-1 (AQP-1) (Aquaporin-CHIP) (W |
| 34 | Sinorhizobium medicae WSM419 | 33.10 | 1 | conserved hypothetical transmembrane protein |
| 35 | Synechococcus sp. JA-2-3B'a(2-13) | 33.10 | 1 | TM2 domain protein |
| 36 | Anguilla anguilla | 33.10 | 1 | aquaporin 1 |
| 37 | Sinorhizobium meliloti 1021 | 32.70 | 1 | hypothetical protein SMc01213 |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#||||||||||||||||||||||||| Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |
| Rattus norvegicus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#||||||||||||||||||||||||| Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |
| N/A | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#||||||||||||||||||||||||| Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |
| Canis lupus familiaris | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#||||||||||||||||||||||||| Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |
| Rattus rattus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#||||||||||||||||||||||||| Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |
| Homo sapiens | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#|||||||||||||||||||| |||| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELNTQAL 263 |
| Oryctolagus cuniculus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |||||| |||||||||||||||||||||||#||||||||||||||||||||||||| Sbjct 209 IIGGGLASLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |
| Cavia porcellus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ||||||||||||||||||||||||||||||#| ||||+|||||||||||||||||| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GTRPSDNNGQPEGTGEPVELKTQAL 263 |
| Bos taurus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 +|| ||||||||||||||||||||||||||#|+|||+|||||| |||||||||||| Sbjct 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 |
| Macaca mulatta | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |||||||||||||||||||||+|||||+|+#| +| |+||||| ||||||| |||| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSISERLSVLR#GVKPEDANGQPEVTGEPVELNTQAL 263 |
| Monodelphis domestica | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |||| || ||||||||||+|||||||+|||#| ||+|| |||| |||||||||||| Sbjct 217 IIGGTLGGLLYDFLLFPRIKSVSERLTILK#GIRPNDSEGQPEVTGEPVELKTQAL 271 |
| Rana pipiens | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |||| || |+|||+||||++ +||||||||#||||++ || | ||||+|||||+| Sbjct 208 IIGGALGGLVYDFILFPRMRGLSERLSILK#GARPAEPEGQQEATGEPIELKTQSL 262 |
| Tetraodon nigroviridis | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 +||| +|+|||||+||||++ +||||+ ||#| || ++ || | |||+||||||| Sbjct 209 MIGGAMGALLYDFMLFPRMRGLSERLATLK#GTRPPEAEGQQETRGEPIELKTQAL 263 |
| Xenopus laevis | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |||| +| |+|||+||||++ ++|| ||||#||||+ || | |+|+|||||+| Sbjct 210 IIGGAVGGLVYDFILFPRMRGLNERFSILK#GARPAVPEGQRETAGDPIELKTQSL 264 |
| Xenopus tropicalis | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 |||| +| |+|||+||||++ ++|||||||#||||++ || | +|+|||||+| Sbjct 210 IIGGAVGGLVYDFILFPRMRGLNERLSILK#GARPAEPEGQRETIRDPIELKTQSL 264 |
| Danio rerio | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 +|| +|+|||||+||||++ +||||++||#| +|++ | | |||+||||||| Sbjct 209 MIGAAMGALLYDFMLFPRVRGLSERLAVLK#GNKPTEPEAQQETRGEPIELKTQAL 263 |
| Fundulus heteroclitus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 +||| +|+||||||||||++ +||||+ ||#| | ++ | | || +||||||| Sbjct 209 MIGGAIGALLYDFLLFPRMRGLSERLATLK#GTRTPEAEAQQETRGESIELKTQAL 263 |
| Gallus gallus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263 ++| | +|||+ | || +|++|||++|+#| |+ + || ||+||||| | Sbjct 209 LLGAALAALLYELALCPRARSMAERLAVLR#GEPPA-AAPPPEPPAEPLELKTQGL 262 |
| Pan troglodytes | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARP 242 ++| ||||||+++||| ||+||||++||#| | Sbjct 201 LVGAILGSLLYNYVLFPPAKSLSERLAVLK#GLEP 234 |
| synthetic construct | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARP 242 ++| ||||||+++||| ||+||||++||#| | Sbjct 209 LVGAILGSLLYNYVLFPPAKSLSERLAVLK#GLEP 242 |
| human, kidney, Peptide, 271 aa | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARP 242 ++| ||||||+++||| ||+||||++||#| | Sbjct 209 LVGAILGSLLYNYVLFPPAKSLSERLAVLK#GLEP 242 |
| Notomys alexis | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARP 242 ++| +|||||++|||| ||+ |||++||#| | Sbjct 89 LVGAIIGSLLYNYLLFPSAKSLQERLAVLK#GLEP 122 |
| Canis familiaris | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARP 242 ++| ||||||+++||| ||++|||++||#| | Sbjct 209 LVGAILGSLLYNYVLFPPAKSLAERLAVLK#GLEP 242 |
| Ovis aries | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARP 242 ++| + ||||+++||| ||+||||++||#| | Sbjct 209 LVGAIVASLLYNYVLFPPAKSLSERLAVLK#GLEP 242 |
| Bufo marinus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#G 239 ++|| | ||||++||+| ||+|||+++||#| Sbjct 209 LVGGILASLLYNYLLYPYNKSLSERIAVLK#G 239 |
| Coturnix coturnix | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPE 250 ++| |++|+++|||+ |+ ||||+| |#| | + + | Sbjct 210 LVGAAAASIIYNYILFPQSKTFSERLAIFK#GMEPEEDWAERE 251 |
| Hyla chrysoscelis | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#G 239 + | +|||+|+|+| | |+ |||++||+#| Sbjct 214 LFGATVGSLMYNFILIPNTKTFSERIAILR#G 244 |
| Equus caballus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#G 239 |+| | ++|| +|||| |+|||++|+|#| Sbjct 28 IVGAALAAILYFYLLFPNSLSLSERVAIVK#G 58 |
| Aeromonas hydrophila subsp. hydrophila ATCC 7966 | Query 209 IIGGG----LGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGE 254 +|||| || | || | +| +|| |++#|| | ||+ ||+ Sbjct 274 VIGGGTPGAFGSYRDGFELFGRARSPQKRLQIVE#GASHYDLYDQPDATGQ 323 |
| Passer domesticus | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELK 259 |||| +|+|||+| || +++|+ + # + + + + + ||+| Sbjct 219 IIGGASAALIYDFILAPRTSDLTDRMKVWT#SGQVEEYDLEGDDMNSRVEMK 269 |
| Hyla japonica | Query 210 IGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPS 243 +| | ||+|+++| |+ +| ||+||+| #| |+ Sbjct 210 VGAILASLIYNYVLCPQEQSFSEKLSVLL#GRIPA 243 |
| Sus scrofa | Query 210 IGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELK 259 ||| | |+|||+| || +++|+ + # + + + + ||+| Sbjct 220 IGGALAVLIYDFILAPRSSDLTDRVKVWT#SGQVEEYDLDGDDINSRVEMK 269 |
| Pongo pygmaeus | Query 210 IGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELK 259 ||| | |+|||+| || ++|+ + # + + + + ||+| Sbjct 218 IGGALAVLIYDFILAPRSSDFTDRVKVWT#SGQVEEYDLDADDINSRVEMK 267 |
| Sinorhizobium medicae WSM419 | Query 211 GGGL--GSLLYDFLLFPRLKSVS-ERLSILK#GARP--------SDSNGQPEGTGEPVELK 259 |||+ |+|++ +|+ ||++ + | +|+#| | || +| |+|| | |+| Sbjct 45 GGGMSFGTLIFLVILYFVLKAMGIDMLQVLE#GGGPANMPGFEQSDGSGAPQGTAEEEEMK 104 |
| Synechococcus sp. JA-2-3B'a(2-13) | Query 213 GLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVE-LKTQAL 263 | | |+ ||+ +++ + +|++|+#|+||| | + | | + || ||| Sbjct 54 GFGQLIDLFLIPGMVENYNFKLAVLQ#GSRPSPSPASSKNTSAPPQPLKGQAL 105 |
| Anguilla anguilla | Query 209 IIGGGLGSLLYDFLLFPRLKSVSERLSIL 237 | || + +|+||+|| |+| ++||| +| Sbjct 209 ISGGLVAALVYDYLLHPKLGDLTERLKVL 237 |
| Sinorhizobium meliloti 1021 | Query 211 GGGL--GSLLYDFLLFPRLKSVS-ERLSILK#GARP--------SDSNGQPEGTGEPVELK 259 |||+ |+|++ +|+ ||++ + | +|+#| | || +| |+|| | |+| Sbjct 45 GGGMSFGTLIFLVILYFVLKAMGIDLLQVLE#GGGPANMPGFEQSDGSGAPQGTAEEEEMK 104 |
* References
[PubMed ID: 18757513] Saint-Geniez M, Kurihara T, D'Amore PA, Role of cell and matrix-bound VEGF isoforms in lens development. Invest Ophthalmol Vis Sci. 2009 Jan;50(1):311-21. Epub 2008 Aug 29.
[PubMed ID: 18250320] ... Matteson PG, Desai J, Korstanje R, Lazar G, Borsuk TE, Rollins J, Kadambi S, Joseph J, Rahman T, Wink J, Benayed R, Paigen B, Millonig JH, The orphan G protein-coupled receptor, Gpr161, encodes the vacuolated lens locus and controls neurulation and lens development. Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2088-93. Epub 2008 Feb 4.
[PubMed ID: 18198336] ... Martins RA, Zindy F, Donovan S, Zhang J, Pounds S, Wey A, Knoepfler PS, Eisenman RN, Roussel MF, Dyer MA, N-myc coordinates retinal growth with eye size during mouse development. Genes Dev. 2008 Jan 15;22(2):179-93.
[PubMed ID: 17493607] ... Simirskii VN, Wang Y, Duncan MK, Conditional deletion of beta1-integrin from the developing lens leads to loss of the lens epithelial phenotype. Dev Biol. 2007 Jun 15;306(2):658-68. Epub 2007 Apr 12.
[PubMed ID: 17410548] ... Kreslova J, Machon O, Ruzickova J, Lachova J, Wawrousek EF, Kemler R, Krauss S, Piatigorsky J, Kozmik Z, Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function. Genesis. 2007 Apr;45(4):157-68.
[PubMed ID: 1511869] ... Kratochvilova J, Favor J, Allelism tests of 15 dominant cataract mutations in mice. Genet Res. 1992 Jun;59(3):199-203.
[PubMed ID: 1544329] ... Griffin CS, Shiels A, Localisation of the gene for the major intrinsic protein of eye-lens-fibre cell membranes to mouse chromosome 10 by in situ hybridisation. Cytogenet Cell Genet. 1992;59(4):300-2.
[PubMed ID: 1505237] ... Griffin CS, Shiels A, In situ hybridisation localises the gene for the major intrinsic protein of eye lens fibre cell membranes to human chromosome 12q14. Cytogenet Cell Genet. 1992;61(1):8-9.
[PubMed ID: 1677593] ... Shiels A, Griffin CS, Muggleton-Harris AL, Restriction fragment length polymorphisms associated with the gene for the major intrinsic protein of eye-lens fibre cell membranes in mice with hereditary cataracts. Biochim Biophys Acta. 1991 Jul 26;1097(1):81-5.
[PubMed ID: 1026873] ... Koniukhov BV, Kolesova NA, [Ultrastructural analysis of the effects of dominant cataract-Fr gene in mouse embryos] Ontogenez. 1976;7(3):271-7.