CaMPDB :: Substrate

XSB1595 : major intrinsic protein of lens fiber [Bos taurus]

This entry is computationally expanded from SB0003

* Basic Information

Organism	Bos taurus (cattle)
Protein Names	Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26; major intrinsic protein of lens fiber; aquaporin O
Gene Names	MIP; major intrinsic protein of lens fiber
Gene Locus	5; chromosome 5
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_776362	NM_173937	280859	P06624	N/A	N/A	N/A	bta:280859

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 263 aa

Average Mass: 28.223 kDa

Monoisotopic Mass: 28.205 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
MIP 1.	3	219	430.6	2.5e-126

3. Sequence Information

Fasta Sequence: XSB1595.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1YMG (X-ray; 224 A; A=1-263), 2B6P (X-ray; 240 A; A=1-263), 2C32 (X-ray; 701 A; A=1-263)

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] SVSERLSILK²³⁸-GSRPSESNGQ

Lys²³⁸ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ser²²⁹	Val²³⁰	Ser²³¹	Glu²³²	Arg²³³	Leu²³⁴	Ser²³⁵	Ile²³⁶	Leu²³⁷	Lys²³⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly²³⁹	Ser²⁴⁰	Arg²⁴¹	Pro²⁴²	Ser²⁴³	Glu²⁴⁴	Ser²⁴⁵	Asn²⁴⁶	Gly²⁴⁷	Gln²⁴⁸

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
VIGAGLGSLLYDFLLFPRLKSVSERLSILKGSRPSESNGQPEVTGEPVELKTQAL

Summary

#	organism	max score	hits	top seq
1	Bos taurus	108.00	5	major intrinsic protein of lens fiber
2	N/A	107.00	5	M
3	Mus musculus	101.00	12	Major intrinsic protein of eye lens fiber
4	Rattus norvegicus	101.00	7	PREDICTED: similar to major intrinsic protein of e
5	Rattus rattus	101.00	2	MIP_RAT Lens fiber major intrinsic protein (Aquapo
6	Canis lupus familiaris	101.00	1	major intrinsic protein of lens fiber
7	Cavia porcellus	100.00	1	MIP_CAVPO Lens fiber major intrinsic protein (Aqua
8	Homo sapiens	99.80	9	putative alternative lens membrane intrinsic prote
9	Oryctolagus cuniculus	99.40	1	aquaporin
10	Macaca mulatta	95.50	2	PREDICTED: major intrinsic protein of lens fiber
11	Monodelphis domestica	93.20	2	PREDICTED: similar to lens-specific aquaporin-0; M
12	Rana pipiens	83.60	1	CAA40291.1
13	Tetraodon nigroviridis	79.30	3	unnamed protein product
14	Danio rerio	78.60	4	major intrinsic protein of lens fiber 1
15	Xenopus tropicalis	74.30	2	hypothetical protein LOC100037914
16	Xenopus laevis	73.60	4	hypothetical protein LOC495140
17	Fundulus heteroclitus	71.60	1	AF191906_1 lens major intrinsic protein
18	Gallus gallus	53.10	4	major intrinsic protein of lens fiber
19	Pan troglodytes	47.80	5	PREDICTED: aquaporin 2 isoform 3
20	synthetic construct	47.80	3	aquaporin 2
21	human, kidney, Peptide, 271 aa	47.80	1	hAQP-CD=collecting duct aquaporin
22	Notomys alexis	47.00	1	aquaporin 2
23	Canis familiaris	46.60	4	PREDICTED: similar to aquaporin 2
24	Ovis aries	44.30	3	AQP2_SHEEP Aquaporin-2 (AQP-2) (Aquaporin-CD) (AQP
25	Coturnix coturnix	43.50	1	aquaporin-2
26	Bufo marinus	40.40	2	water channel homolog
27	Equus caballus	40.00	1	aquaporin 5
28	Hyla chrysoscelis	39.30	1	aquaporin 2-like
29	Hyla japonica	35.40	1	water channel protein AQP-h2
30	Synechococcus sp. JA-2-3B'a(2-13)	33.10	1	TM2 domain protein
31	Passer domesticus	33.10	1	aquaporin-1

Top-ranked sequences

organism	matching
Bos taurus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263
N/A	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GTRPSESNGQPEVTGEPVELKTQAL 263
Mus musculus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263
Rattus norvegicus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263
Rattus rattus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 207 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 261
Canis lupus familiaris	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263
Cavia porcellus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|++\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GTRPSDNNGQPEGTGEPVELKTQAL 263
Homo sapiens	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELNTQAL 263
Oryctolagus cuniculus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|+\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 209 IIGGGLASLLYDFLLFPRLKSVSERLSILK#GARPSDSNGQPEGTGEPVELKTQAL 263
Macaca mulatta	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\|+#\| +\| ++\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 209 IIGGGLGSLLYDFLLFPRLKSISERLSVLR#GVKPEDANGQPEVTGEPVELNTQAL 263
Monodelphis domestica	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\| \|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|+\|\|\|#\| \|\|++\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 217 IIGGTLGGLLYDFLLFPRIKSVSERLTILK#GIRPNDSEGQPEVTGEPVELKTQAL 271
Rana pipiens	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| \|\| \|+\|\|\|+\|\|\|\|++ +\|\|\|\|\|\|\|\|#\|+\|\|+\| \|\| \| \|\|\|\|+\|\|\|\|\|+\| Sbjct 208 IIGGALGGLVYDFILFPRMRGLSERLSILK#GARPAEPEGQQEATGEPIELKTQSL 262
Tetraodon nigroviridis	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| +\|+\|\|\|\|\|+\|\|\|\|++ +\|\|\|\|+ \|\|#\|+\|\| \|+ \|\| \| \|\|\|+\|\|\|\|\|\|\| Sbjct 209 MIGGAMGALLYDFMLFPRMRGLSERLATLK#GTRPPEAEGQQETRGEPIELKTQAL 263
Danio rerio	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\|\| +\|+\|\|\|\|\|+\|\|\|\|++ +\|\|\|\|++\|\|#\|++\|+\| \| \| \|\|\|+\|\|\|\|\|\|\| Sbjct 209 MIGAAMGALLYDFMLFPRVRGLSERLAVLK#GNKPTEPEAQQETRGEPIELKTQAL 263
Xenopus tropicalis	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| +\| \|+\|\|\|+\|\|\|\|++ ++\|\|\|\|\|\|\|#\|+\|\|+\| \|\| \| +\|+\|\|\|\|\|+\| Sbjct 210 IIGGAVGGLVYDFILFPRMRGLNERLSILK#GARPAEPEGQRETIRDPIELKTQSL 264
Xenopus laevis	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| +\| \|+\|\|\|+\|\|\|\|++ ++\|\| \|\|\|\|#\|+\|\|+ \|\| \| \|+\|+\|\|\|\|\|+\| Sbjct 210 IIGGAVGGLVYDFILFPRMRGLNERFSILK#GARPAVPEGQRETAGDPIELKTQSL 264
Fundulus heteroclitus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 +\|\| +\|+\|\|\|\|\|\|\|\|\|\|++ +\|\|\|\|+ \|\|#\|+\| \|+ \| \| \|\| +\|\|\|\|\|\|\| Sbjct 209 MIGGAIGALLYDFLLFPRMRGLSERLATLK#GTRTPEAEAQQETRGESIELKTQAL 263
Gallus gallus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELKTQAL 263 ++\|\| \| +\|\|\|+ \| \|\| +\|++\|\|\|++\|+#\| \|+ + \|\| \|\|+\|\|\|\|\| \| Sbjct 209 LLGAALAALLYELALCPRARSMAERLAVLR#GEPPAAA-PPPEPPAEPLELKTQGL 262
Pan troglodytes	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEV 251 ++\|\| \|\|\|\|\|\|+++\|\|\| \|\|+\|\|\|\|++\|\|#\| \| + \|\| Sbjct 201 LVGAILGSLLYNYVLFPPAKSLSERLAVLK#GLEPDTDWEEREV 243
synthetic construct	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEV 251 ++\|\| \|\|\|\|\|\|+++\|\|\| \|\|+\|\|\|\|++\|\|#\| \| + \|\| Sbjct 209 LVGAILGSLLYNYVLFPPAKSLSERLAVLK#GLEPDTDWEEREV 251
human, kidney, Peptide, 271 aa	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEV 251 ++\|\| \|\|\|\|\|\|+++\|\|\| \|\|+\|\|\|\|++\|\|#\| \| + \|\| Sbjct 209 LVGAILGSLLYNYVLFPPAKSLSERLAVLK#GLEPDTDWEEREV 251
Notomys alexis	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEV 251 ++\|\| +\|\|\|\|\|++\|\|\|\| \|\|+ \|\|\|++\|\|#\| \| + \|\| Sbjct 89 LVGAIIGSLLYNYLLFPSAKSLQERLAVLK#GLEPDTDWEEREV 131
Canis familiaris	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEV 251 ++\|\| \|\|\|\|\|\|+++\|\|\| \|\|++\|\|\|++\|\|#\| \| + \|\| Sbjct 209 LVGAILGSLLYNYVLFPPAKSLAERLAVLK#GLEPDADWEEREV 251
Ovis aries	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEV 251 ++\|\| + \|\|\|\|+++\|\|\| \|\|+\|\|\|\|++\|\|#\| \| + \|\| Sbjct 209 LVGAIVASLLYNYVLFPPAKSLSERLAVLK#GLEPDTDWEEREV 251
Coturnix coturnix	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPE 250 ++\|\| \|++\|+++\|\|\|+ \|+ \|\|\|\|+\| \|#\| \| \| + \| Sbjct 210 LVGAAAASIIYNYILFPQSKTFSERLAIFK#GMEPEEDWAERE 251
Bufo marinus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSE 244 ++\| \| \|\|\|\|++\|\|+\| \|\|+\|\|\|+++\|\|#\|+ \| Sbjct 209 LVGGILASLLYNYLLYPYNKSLSERIAVLK#GTYEPE 244
Equus caballus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPE 250 ++\|\| \| ++\|\| +\|\|\|\| \|+\|\|\|++\|+\|#\|+ \| + + + Sbjct 28 IVGAALAAILYFYLLFPNSLSLSERVAIVK#GTYEPEEDWEEQ 69
Hyla chrysoscelis	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#G 239 + \|\| +\|\|\|+\|+\|+\| \| \|+ \|\|\|++\|\|+#\| Sbjct 214 LFGATVGSLMYNFILIPNTKTFSERIAILR#G 244
Hyla japonica	Query 210 IGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPS 243 +\|\| \| \|\|+\|+++\| \|+ +\| \|\|+\|\|+\| #\| \|+ Sbjct 210 VGAILASLIYNYVLCPQEQSFSEKLSVLL#GRIPA 243
Synechococcus sp. JA-2-3B'a(2-13)	Query 213 GLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVE-LKTQAL 263 \| \| \|+ \|\|+ +++ + +\|++\|+#\|\|\|\|\| \| + \| \| + \|\| \|\|\| Sbjct 54 GFGQLIDLFLIPGMVENYNFKLAVLQ#GSRPSPSPASSKNTSAPPQPLKGQAL 105
Passer domesticus	Query 209 VIGAGLGSLLYDFLLFPRLKSVSERLSILK#GSRPSESNGQPEVTGEPVELK 259 +\|\| +\|+\|\|\|+\| \|\| +++\|+ + # + \| + + + \|\|+\| Sbjct 219 IIGGASAALIYDFILAPRTSDLTDRMKVWT#SGQVEEYDLEGDDMNSRVEMK 269

* References

[PubMed ID: 16309700] Palanivelu DV, Kozono DE, Engel A, Suda K, Lustig A, Agre P, Schirmer T, Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D crystals. J Mol Biol. 2006 Jan 27;355(4):605-11. Epub 2005 Nov 8.

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