* Structure Information
1. Primary Information
Length: 5035 aa
Average Mass: 565.039 kDa
Monoisotopic Mass: 564.679 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| Ins145_P3_rec 1. | 10 | 212 | 360.3 | 3.5e-105 |
| MIR 1. | 213 | 392 | 264.7 | 2.2e-76 |
| RYDR_ITPR 1. | 441 | 645 | 331.7 | 1.4e-96 |
| SPRY 1. | 660 | 798 | 139.4 | 1.1e-38 |
| RyR 1. | 851 | 945 | 220.8 | 3.5e-63 |
| RyR 2. | 965 | 1059 | 212.3 | 1.3e-60 |
| SPRY 2. | 1086 | 1209 | 163.5 | 6.4e-46 |
| --- cleavage 1401 --- |
| SPRY 3. | 1431 | 1571 | 153.4 | 6.7e-43 |
| Phage_XkdX 1. | 1844 | 1878 | -11.2 | 8.4 |
| Cenp-B_dimeris 1. | 1882 | 1984 | -27.3 | 6.1 |
| Hep_59 1. | 2032 | 2101 | -42.0 | 6.1 |
| RYDR_ITPR 2. | 2158 | 2366 | 306.7 | 5e-89 |
| RyR 3. | 2735 | 2829 | 217.2 | 4.3e-62 |
| --- cleavage 2844 --- |
| RyR 4. | 2855 | 2943 | 180.1 | 6.2e-51 |
| Hpt 1. | 3091 | 3165 | -9.0 | 6 |
| Transposase_12 1. | 3327 | 3387 | -55.7 | 3.1 |
| DUF1081 1. | 3506 | 3595 | -30.9 | 3 |
| DUF1911 1. | 3665 | 3761 | -51.5 | 8.1 |
| HTH_DeoR 1. | 3816 | 3865 | -17.5 | 5.4 |
| RIH_assoc 1. | 3874 | 4007 | 326.0 | 7.8e-95 |
| CHCH 1. | 4102 | 4141 | -5.9 | 7.8 |
| HR1 1. | 4191 | 4264 | -10.9 | 2.1 |
| HTH_1 1. | 4302 | 4351 | 1.6 | 2.1 |
| RR_TM4-6 1. | 4379 | 4668 | 405.1 | 1.1e-118 |
| Ion_trans 1. | 4759 | 4934 | 37.6 | 4.9e-08 |
| zf-C2H2 1. | 4954 | 4981 | 2.3 | 6.9 |
3. Sequence Information
Fasta Sequence: XSB1608.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] TEKKKTRKIS2844-QTAQTYDPRE
Ser2844 
Gln
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Thr2835 | Glu2836 | Lys2837 | Lys2838 | Lys2839 | Thr2840 | Arg2841 | Lys2842 | Ile2843 | Ser2844 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln2845 | Thr2846 | Ala2847 | Gln2848 | Thr2849 | Tyr2850 | Asp2851 | Pro2852 | Arg2853 | Glu2854 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
KAMIAWEWTVEKAREGEEEKTEKKKTRKISQTAQTYDPREGYNPQPPDLSVVTLSRELQA
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Mus musculus |
83.60 |
4 |
ryanodine receptor |
| 2 |
Rattus norvegicus |
83.60 |
2 |
PREDICTED: similar to ryanodine receptor 1, skelet |
| 3 |
N/A |
80.50 |
6 |
1 |
| 4 |
Oryctolagus cuniculus |
80.50 |
2 |
RYR1_RABIT Ryanodine receptor 1 (Skeletal muscle-t |
| 5 |
Homo sapiens |
80.10 |
11 |
skeletal muscle ryanodine receptor isoform 2 |
| 6 |
Sus scrofa |
75.90 |
4 |
ryanodine receptor |
| 7 |
Bos taurus |
63.90 |
2 |
PREDICTED: similar to Ryanodine receptor 1 (Skelet |
| 8 |
Danio rerio |
62.00 |
2 |
PREDICTED: similar to ryanodine receptor RyR1 |
| 9 |
Tetraodon nigroviridis |
59.30 |
3 |
unnamed protein product |
| 10 |
Prot=RyR1 isform from fish |
58.20 |
1 |
- |
| 11 |
Macaca mulatta |
55.10 |
1 |
PREDICTED: similar to ryanodine receptor 1 (skelet |
| 12 |
Rana catesbeiana |
45.10 |
1 |
ryanodine receptor alpha isoform |
| 13 |
Hemicentrotus pulcherrimus |
37.70 |
1 |
ryanodine receptor |
| 14 |
Canis familiaris |
37.00 |
1 |
PREDICTED: similar to Ryanodine receptor 2 (Cardia |
| 15 |
Pan troglodytes |
37.00 |
1 |
PREDICTED: cardiac muscle ryanodine receptor |
| 16 |
Monodelphis domestica |
37.00 |
1 |
PREDICTED: similar to cardiac muscle ryanodine rec |
| 17 |
Gallus gallus |
35.80 |
1 |
PREDICTED: similar to cardiac muscle ryanodine rec |
Top-ranked sequences
| organism | matching |
|---|
| Mus musculus |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|||||||||| ||#||||||||||||||||||||||||||||||
Sbjct 2815 KAMIAWEWTVEKAREGEEEKTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|
| Rattus norvegicus |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|||||||||| ||#||||||||||||||||||||||||||||||
Sbjct 2815 KAMIAWEWTVEKAREGEEEKTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|
| N/A |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|||||||||+ ||#|||||||||||||||||||| |||||||||
Sbjct 2814 KAMIAWEWTIEKAREGEEERTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSGVTLSRELQA 2873
|
| Oryctolagus cuniculus |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|||||||||+ ||#|||||||||||||||||||| |||||||||
Sbjct 2814 KAMIAWEWTIEKAREGEEERTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSGVTLSRELQA 2873
|
| Homo sapiens |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|||||||||+ ||#|+|||||||||||||||||| |||||||||
Sbjct 2814 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDPREGYNPQPPDLSAVTLSRELQA 2873
|
| Sus scrofa |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|||||||||+ ||#|+||||| |||||||||||| |||||||||
Sbjct 2815 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|
| Bos taurus |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVT 2867
|||||||||+ ||#|+||||| |||||||||||| ||
Sbjct 966 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVT 1018
|
| Danio rerio |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
|||||||||+ ||#|||| |||| ||+||| |+| +|||||||
Sbjct 2852 KAMIAWEWTLDKARDGEDEKVEKKTARKIS#QTAQATYDPSHGYSPQPIDISGMTLSRELQ 2911
Query 2874 A 2874
+
Sbjct 2912 S 2912
|
| Tetraodon nigroviridis |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
|||+|||||+ ||#|||| |||| ||+||| |+| + ||||||
Sbjct 2341 KAMLAWEWTLEKARDGEGEVEKKAATRKIS#QTAQATYDPSHGYSPQPIDISGMALSRELQ 2400
Query 2874 A 2874
+
Sbjct 2401 S 2401
|
| Prot=RyR1 isform from fish |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
|||+|||| + ||#|||| |||| ||+||| |+| | |||+||
Sbjct 2853 KAMLAWEWNIEKAREEEESEKKKATSRKIS#QTAQATYDPSHGYSPQPIDISHVALSRDLQ 2912
Query 2874 A 2874
+
Sbjct 2913 S 2913
|
| Macaca mulatta |
Query 2848 QTYDPREGYNPQPPDLSVVTLSRELQ 2873
+|||||||||||||||| ||||||||
Sbjct 820 KTYDPREGYNPQPPDLSAVTLSRELQ 845
|
| Rana catesbeiana |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXX-IS#QTAQ-TYDPR-EGYNPQPPDLSVVTLSRE 2871
|||||||| | ||#|||| |||| + ++| | ||+ +|||||
Sbjct 2800 KAMIAWEWLVEKAREGEEDRTEKKTKTRKIS#QTAQQTYDPMVQTFSPTPIDLTGLTLSRE 2859
Query 2872 LQA 2874
||+
Sbjct 2860 LQS 2862
|
| Hemicentrotus pulcherrimus |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQ 2873
||++|| |+| # + + ||||+| |+| +||+||+|
Sbjct 2985 KALLAWGWSVERSKASEQQSQAHRPRRLSK#ASLGAMESPHGYNPRPIDVSNITLTREMQ 3043
|
| Canis familiaris |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
| |+|| | + ||#||+| + | ||+|+ |+| |||||+|
Sbjct 2774 KTMLAWGWRIERTREGDSMALYNRTRR-IS#QTSQVSVDAAHGYSPRAIDMSNVTLSRDLH 2832
Query 2874 A 2874
|
Query 2874 A 2874
|
| Pan troglodytes |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
| |+|| | + ||#||+| + | ||+|+ |+| |||||+|
Sbjct 2826 KTMLAWGWRIERTREGDSMALYNRTRR-IS#QTSQVSVDAAHGYSPRAIDMSNVTLSRDLH 2884
Query 2874 A 2874
|
Query 2874 A 2874
|
| Monodelphis domestica |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
| |+|| | + ||#||+| + | ||+|+ |+| |||||+|
Sbjct 2850 KTMLAWGWRIERTREGDSMALYTRTRR-IS#QTSQVSIDTAHGYSPRAIDMSNVTLSRDLH 2908
Query 2874 A 2874
|
Query 2874 A 2874
|
| Gallus gallus |
Query 2815 KAMIAWEWTVXXXXXXXXXXXXXXXXXXIS#QTAQ-TYDPREGYNPQPPDLSVVTLSRELQ 2873
| |+|| | + ||#||+| + | ||+|+ |+| |||||++
Sbjct 614 KTMLAWGWRIERTREGDSMALYNRTRR-IS#QTSQISVDAAHGYSPRAIDMSNVTLSRDVH 672
Query 2874 A 2874
|
Query 2874 A 2874
|
[Site 2] FKAKKVAMMT1401-QPPSTPALPR
Thr1401 Gln
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Phe1392 | Lys1393 | Ala1394 | Lys1395 | Lys1396 | Val1397 | Ala1398 | Met1399 | Met1400 | Thr1401 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln1402 | Pro1403 | Pro1404 | Ser1405 | Thr1406 | Pro1407 | Ala1408 | Leu1409 | Pro1410 | Arg1411 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
ARAENEKDSTTEKNKKRGFLFKAKKVAMMTQPPSTPALPRLPRDVVPADNRDDPEIILNT
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Mus musculus |
123.00 |
2 |
ryanodine receptor |
| 2 |
Rattus norvegicus |
122.00 |
2 |
PREDICTED: similar to ryanodine receptor 1, skelet |
| 3 |
Homo sapiens |
117.00 |
6 |
skeletal muscle ryanodine receptor isoform 2 |
| 4 |
N/A |
117.00 |
4 |
- |
| 5 |
Oryctolagus cuniculus |
116.00 |
1 |
RYR1_RABIT Ryanodine receptor 1 (Skeletal muscle-t |
| 6 |
Monodelphis domestica |
114.00 |
1 |
PREDICTED: hypothetical protein |
| 7 |
Sus scrofa |
110.00 |
4 |
ryanodine receptor |
| 8 |
Tetraodon nigroviridis |
79.70 |
2 |
unnamed protein product |
| 9 |
Danio rerio |
78.60 |
2 |
PREDICTED: hypothetical protein, partial |
| 10 |
Rana catesbeiana |
77.80 |
1 |
ryanodine receptor alpha isoform |
| 11 |
Prot=RyR1 isform from fish |
65.90 |
1 |
- |
| 12 |
Tetrahymena thermophila SB210 |
35.00 |
1 |
hypothetical protein TTHERM_00473160 |
| 13 |
Chloroflexus aurantiacus J-10-fl |
34.70 |
1 |
Extensin-like protein |
| 14 |
Trypanosoma brucei |
34.30 |
1 |
hypothetical protein, conserved |
| 15 |
Verminephrobacter eiseniae EF01-2 |
33.50 |
1 |
outer membrane protein |
Top-ranked sequences
| organism | matching |
|---|
| Mus musculus |
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|
| Rattus norvegicus |
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||||||||+|||||||||||||||||||||#||||||||||||||||||||||||||||||
Sbjct 1372 ARAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|
| Homo sapiens |
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||||||||+|||||||||||||||||||||#|||+|| ||||| |||||||||||||||||
Sbjct 1370 ARAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPATPTLPRLPHDVVPADNRDDPEIILNT 1429
|
| N/A |
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||||||||+|||||||||||||||||||||#|||+|| ||||| |||||||||||||||||
Sbjct 1369 ARAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPATPTLPRLPHDVVPADNRDDPEIILNT 1428
|
| Oryctolagus cuniculus |
Query 1373 RAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|||||||+|||||||||||||||| ||||#|||+|||||||| |||||||||||||||||
Sbjct 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPALPRLPHDVVPADNRDDPEIILNT 1430
|
| Monodelphis domestica |
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||||||||| |||+||||||||||| ||||#| |+||||||||||||||||||||||||||
Sbjct 1298 ARAENEKDSATEKSKKRGFLFKAKKAAMMT#QAPATPALPRLPRDVVPADNRDDPEIILNT 1357
|
| Sus scrofa |
Query 1373 RAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|||||||+|||||||||||||||| ||||#|||+|| ||||| +|||||+||||+|||||
Sbjct 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|
| Tetraodon nigroviridis |
Query 1375 ENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|||||++|||+||+|||||||| |++ # || | +||| |||| |+||| +|||||
Sbjct 1329 ENEKDASTEKSKKKGFLFKAKKAALIN#PPPVVPTMPRLVEDVVP-DDRDDVDIILNT 1384
|
| Danio rerio |
Query 1375 ENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||+||+ +|| ||+|||||||| ||+|# || | +||| |||| |+||| +|||||
Sbjct 355 ENDKDTASEKGKKKGFLFKAKKAAMIT#PPPPPPLMPRLIEDVVP-DDRDDVDIILNT 410
|
| Rana catesbeiana |
Query 1373 RAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
+ |||||+|||| |||||||| || | ++# | | +||| ++||| |+||||||| ||
Sbjct 1362 KPENEKDTTTEKTKKRGFLFKPKKPAFIS#TTPVVPTMPRLMKEVVP-DDRDDPEIIRNT 1419
|
| Prot=RyR1 isform from fish |
Query 1375 ENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
||||| | ++| ||||||| |++|# || | +||| |||| |+||| +|||||
Sbjct 1368 ENEKDPQQRKQEER-FLFKAKKPALIT#PPPVVPTMPRLVEDVVP-DDRDDVDIILNT 1422
|
| Tetrahymena thermophila SB210 |
Query 1372 ARAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPR-DVVPADNRDDPEIILN 1430
+++|+ | + ++++ | |+||++|+ # || |+ | + | + + ++ | ++|
Sbjct 297 SKSEDSNDQSPQESRNRSFIFKSEKILNQL#GLPSAPSSPTAEKQDEILDSSENNEEKLIN 356
Query 1431 T 1431
|
Query 1431 T 1431
|
| Chloroflexus aurantiacus J-10-fl |
Query 1373 RAENEKDSTTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIIL 1429
|+| |+ + + | ++| #+|| ||+| | | || + | | ||
Sbjct 861 RSERSADAQRGRYNRPGGRHSGRRVPDQP#EPPEAPAVPEPPTDDSPAASVPDAEAIL 917
|
| Trypanosoma brucei |
Query 1392 FKAKKVAMMT#QPPSTPA-LPRLPRDVVPAD 1420
+| +++ +||# +||| |||||||++ +|
Sbjct 42 YKLRRIVLMT#HMAATPATLPRLPRDIIVSD 71
|
| Verminephrobacter eiseniae EF01-2 |
Query 1392 FKAKKVAMMT#QPPSTPALPRLPRDVVPADNRD 1423
| |+ | +|#||| || | | | |+||
Sbjct 637 FAARAVTNLT#QPPVAPATPEAPGAGAPDDDRD 668
|
* References
[PubMed ID: 18003898] Zvaritch E, Depreux F, Kraeva N, Loy RE, Goonasekera SA, Boncompagni S, Kraev A, Gramolini AO, Dirksen RT, Franzini-Armstrong C, Seidman CE, Seidman JG, Maclennan DH, An Ryr1I4895T mutation abolishes Ca2+ release channel function and delays development in homozygous offspring of a mutant mouse line. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18537-42. Epub 2007 Nov 14.
