CaMPDB :: Substrate

XSB1619 : ryanodine receptor 1 [Sus scrofa]

This entry is computationally expanded from SB0015

* Basic Information

Organism	Sus scrofa (pig)
Protein Names	Ryanodine receptor 1; RYR-1; RyR1; Skeletal muscle-type ryanodine receptor; Skeletal muscle calcium release channel
Gene Names	RYR1; CRC
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
CAA49225	N/A	N/A	P16960	N/A	N/A	N/A	ssc:

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 1850 aa

Average Mass: 208.831 kDa

Monoisotopic Mass: 208.698 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
SPRY 1.	1	80	26.4	1.5e-05
--- cleavage 272 ---
SPRY 2.	302	442	153.8	5.2e-43
RNA_polI_A34 1.	643	798	-77.2	5.7
RIIa 1.	810	847	-8.8	6.8
PRA-PH 1.	926	999	-46.8	2.8
RYDR_ITPR 1.	1030	1238	306.7	5e-89
RyR 1.	1607	1701	217.2	4.2e-62
--- cleavage 1716 ---
RyR 2.	1727	1815	184.4	3.2e-52

3. Sequence Information

Fasta Sequence: XSB1619.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites]

Cleavage sites (±10aa)

[Site 1] TEKKKTRKIS¹⁷¹⁶-QSAQTYDARE

Ser¹⁷¹⁶ Gln

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Thr¹⁷⁰⁷	Glu¹⁷⁰⁸	Lys¹⁷⁰⁹	Lys¹⁷¹⁰	Lys¹⁷¹¹	Thr¹⁷¹²	Arg¹⁷¹³	Lys¹⁷¹⁴	Ile¹⁷¹⁵	Ser¹⁷¹⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gln¹⁷¹⁷	Ser¹⁷¹⁸	Ala¹⁷¹⁹	Gln¹⁷²⁰	Thr¹⁷²¹	Tyr¹⁷²²	Asp¹⁷²³	Ala¹⁷²⁴	Arg¹⁷²⁵	Glu¹⁷²⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
KAMIAWEWTIEKAREGEEEKTEKKKTRKISQSAQTYDAREGYNPQPPDLSGVTLSRELQA

Summary

#	organism	max score	hits	top seq
1	N/A	82.80	6	R
2	Sus scrofa	82.80	4	ryanodine receptor 1
3	Oryctolagus cuniculus	79.70	2	RYR1_RABIT Ryanodine receptor 1 (Skeletal muscle-t
4	Homo sapiens	78.60	12	ryanodine receptor 1 (skeletal), isoform CRA_d
5	Mus musculus	75.90	4	ryanodine receptor
6	Rattus norvegicus	75.90	2	PREDICTED: similar to ryanodine receptor 1 (skelet
7	Bos taurus	70.90	2	PREDICTED: similar to Ryanodine receptor 1 (Skelet
8	Danio rerio	61.20	2	PREDICTED: similar to ryanodine receptor RyR1
9	Tetraodon nigroviridis	58.50	3	unnamed protein product
10	Prot=RyR1 isform from fish	54.30	1	-
11	Macaca mulatta	53.10	1	PREDICTED: similar to ryanodine receptor 1 (skelet
12	Rana catesbeiana	43.50	1	ryanodine receptor alpha isoform
13	Hemicentrotus pulcherrimus	40.40	1	ryanodine receptor
14	Pan troglodytes	39.30	1	PREDICTED: cardiac muscle ryanodine receptor
15	Canis familiaris	39.30	1	PREDICTED: similar to Ryanodine receptor 2 (Cardia
16	Monodelphis domestica	37.70	1	PREDICTED: similar to cardiac muscle ryanodine rec
17	Gallus gallus	37.70	1	PREDICTED: similar to cardiac muscle ryanodine rec
18	Strongylocentrotus purpuratus	32.70	1	PREDICTED: similar to ryanodine receptor, partial

Top-ranked sequences

organism	matching
N/A	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \|\|\|\|\|\|\|\|\|\| \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 2815 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
Sus scrofa	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \|\|\|\|\|\|\|\|\|\| \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1687 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746
Oryctolagus cuniculus	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \|\|\|\|\|\|\|\|\|\| \|\|#\|+\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 2814 KAMIAWEWTIEKAREGEEERTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSGVTLSRELQA 2873
Homo sapiens	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \|\|\|\|\|\|\|\|\|\| \|\|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 2814 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDPREGYNPQPPDLSAVTLSRELQA 2873
Mus musculus	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \|\|\|\|\|\|\|\|\|+ \|\|#\|+\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 2815 KAMIAWEWTVEKAREGEEEKTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
Rattus norvegicus	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \|\|\|\|\|\|\|\|\|+ \|\|#\|+\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 2701 KAMIAWEWTVEKAREGEEEKTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2760
Bos taurus	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVT 1739 \|\|\|\|\|\|\|\|\|\| \|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 966 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVT 1018
Danio rerio	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQ-TYDAREGYNPQPPDLSGVTLSRELQ 1745 \|\|\|\|\|\|\|\|\|+ \|\|#\|+\|\| \|\|\| \|\|+\|\|\| \|+\|\|+\|\|\|\|\|\|\| Sbjct 1003 KAMIAWEWTLDKARDGEDEKVEKKTARKIS#QTAQATYDPSHGYSPQPIDISGMTLSRELQ 1062 Query 1746 A 1746 + Sbjct 1063 S 1063
Tetraodon nigroviridis	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQ-TYDAREGYNPQPPDLSGVTLSRELQ 1745 \|\|\|+\|\|\|\|\|+ \|\|#\|+\|\| \|\|\| \|\|+\|\|\| \|+\|\|+ \|\|\|\|\|\| Sbjct 2341 KAMLAWEWTLEKARDGEGEVEKKAATRKIS#QTAQATYDPSHGYSPQPIDISGMALSRELQ 2400 Query 1746 A 1746 + Sbjct 2401 S 2401
Prot=RyR1 isform from fish	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQ-TYDAREGYNPQPPDLSGVTLSRELQ 1745 \|\|\|+\|\|\|\| \| \|\|#\|+\|\| \|\|\| \|\|+\|\|\| \|+\| \| \|\|\|+\|\| Sbjct 2853 KAMLAWEWNIEKAREEEESEKKKATSRKIS#QTAQATYDPSHGYSPQPIDISHVALSRDLQ 2912 Query 1746 A 1746 + Sbjct 2913 S 2913
Macaca mulatta	Query 1718 SAQTYDAREGYNPQPPDLSGVTLSRELQ 1745 \| +\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 818 SEKTYDPREGYNPQPPDLSAVTLSRELQ 845
Rana catesbeiana	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXX-IS#QSAQ-TYDAR-EGYNPQPPDLSGVTLSRE 1743 \|\|\|\|\|\|\|\| + \|\|#\|+\|\| \|\|\| + ++\| \| \|\|+\|+\|\|\|\|\| Sbjct 2800 KAMIAWEWLVEKAREGEEDRTEKKTKTRKIS#QTAQQTYDPMVQTFSPTPIDLTGLTLSRE 2859 Query 1744 LQA 1746 \|\|+ Sbjct 2860 LQS 2862
Hemicentrotus pulcherrimus	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQ 1745 \|\|++\|\| \|++ # \| ++ \|\|\|\|+\| \|+\| +\|\|+\|\|+\| Sbjct 2985 KALLAWGWSVERSKASEQQSQAHRPRRLSK#ASLGAMESPHGYNPRPIDVSNITLTREMQ 3043
Pan troglodytes	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \| \|+\|\| \| \| # \| + \|\| \|\|+\|+ \|+\| \|\|\|\|\|+\| \| Sbjct 2826 KTMLAWGWRIERTREGDSMALYNRTRRISQ#TSQVSVDAAHGYSPRAIDMSNVTLSRDLHA 2885
Canis familiaris	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \| \|+\|\| \| \| # \| + \|\| \|\|+\|+ \|+\| \|\|\|\|\|+\| \| Sbjct 2774 KTMLAWGWRIERTREGDSMALYNRTRRISQ#TSQVSVDAAHGYSPRAIDMSNVTLSRDLHA 2833
Monodelphis domestica	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \| \|+\|\| \| \| # \| + \| \|\|+\|+ \|+\| \|\|\|\|\|+\| \| Sbjct 2850 KTMLAWGWRIERTREGDSMALYTRTRRISQ#TSQVSIDTAHGYSPRAIDMSNVTLSRDLHA 2909
Gallus gallus	Query 1687 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 1746 \| \|+\|\| \| \| # \| + \|\| \|\|+\|+ \|+\| \|\|\|\|\|++ \| Sbjct 614 KTMLAWGWRIERTREGDSMALYNRTRRISQ#TSQISVDAAHGYSPRAIDMSNVTLSRDVHA 673
Strongylocentrotus purpuratus	Query 1723 DAREGYNPQPPDLSGVTLSRELQ 1745 ++ \|\|\|\|+\| \|+\| +\|\|+\|\|+\| Sbjct 4 ESPHGYNPRPIDVSNITLTREMQ 26

[Site 2] FKAKKAAMMT²⁷²-QPPATPTLPR

Thr²⁷² Gln

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe²⁶³	Lys²⁶⁴	Ala²⁶⁵	Lys²⁶⁶	Lys²⁶⁷	Ala²⁶⁸	Ala²⁶⁹	Met²⁷⁰	Met²⁷¹	Thr²⁷²

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gln²⁷³	Pro²⁷⁴	Pro²⁷⁵	Ala²⁷⁶	Thr²⁷⁷	Pro²⁷⁸	Thr²⁷⁹	Leu²⁸⁰	Pro²⁸¹	Arg²⁸²

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
PRAENEKDATTEKNKKRGFLFKAKKAAMMTQPPATPTLPRLPHEVVPADDRDDPDIILNT

Summary

#	organism	max score	hits	top seq
1	N/A	126.00	4	R
2	Sus scrofa	126.00	4	ryanodine receptor 1
3	Homo sapiens	117.00	6	ryanodine receptor 1 (skeletal), isoform CRA_d
4	Oryctolagus cuniculus	117.00	1	RYR1_RABIT Ryanodine receptor 1 (Skeletal muscle-t
5	Rattus norvegicus	111.00	2	PREDICTED: similar to ryanodine receptor 1 (skelet
6	Mus musculus	111.00	2	ryanodine receptor 1, skeletal muscle
7	Monodelphis domestica	105.00	1	PREDICTED: hypothetical protein
8	Tetraodon nigroviridis	87.00	2	unnamed protein product
9	Rana catesbeiana	84.00	1	ryanodine receptor alpha isoform
10	Danio rerio	81.60	2	PREDICTED: hypothetical protein, partial
11	Prot=RyR1 isform from fish	70.90	1	-
12	Verminephrobacter eiseniae EF01-2	33.50	1	outer membrane protein
13	Ashbya gossypii ATCC 10895	32.30	1	AFR489Wp

Top-ranked sequences

organism	matching
N/A	Query 243 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 243 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302
Sus scrofa	Query 243 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 243 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302
Homo sapiens	Query 244 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|+\|\|\|\|+\|\|\|\|\| Sbjct 1371 RAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPATPTLPRLPHDVVPADNRDDPEIILNT 1429
Oryctolagus cuniculus	Query 244 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\| \|\|\|\|\|\|+\|\|\|\|\|+\|\|\|\|+\|\|\|\|\| Sbjct 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPALPRLPHDVVPADNRDDPEIILNT 1430
Rattus norvegicus	Query 244 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|+\|\| \|\|\|\|\| +\|\|\|\|\|+\|\|\|\|+\|\|\|\|\| Sbjct 1360 RAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1418
Mus musculus	Query 244 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|+\|\| \|\|\|\|\| +\|\|\|\|\|+\|\|\|\|+\|\|\|\|\| Sbjct 1373 RAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
Monodelphis domestica	Query 244 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|\|+ \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\| \|\|\|\| \|\|\|\|\| +\|\|\|\|\|+\|\|\|\|+\|\|\|\|\| Sbjct 1299 RAENEKDSATEKSKKRGFLFKAKKAAMMT#QAPATPALPRLPRDVVPADNRDDPEIILNT 1357
Tetraodon nigroviridis	Query 246 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\|\|+\|\|\|+\|\|+\|\|\|\|\|\|\|\|\|\|++ # \|\| \|\|+\|\|\| +\|\|\| \|\|\|\|\| \|\|\|\|\|\| Sbjct 1329 ENEKDASTEKSKKKGFLFKAKKAALIN#PPPVVPTMPRLVEDVVP-DDRDDVDIILNT 1384
Rana catesbeiana	Query 243 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|+ \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \|\| \| ++# \| \|\|+\|\|\| \|\|\|\| \|\|\|\|\|\|+\|\| \|\| Sbjct 1361 PKPENEKDTTTEKTKKRGFLFKPKKPAFIS#TTPVVPTMPRLMKEVVP-DDRDDPEIIRNT 1419
Danio rerio	Query 246 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|+\|\| +\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|+\|# \|\| \| +\|\|\| +\|\|\| \|\|\|\|\| \|\|\|\|\|\| Sbjct 355 ENDKDTASEKGKKKGFLFKAKKAAMIT#PPPPPPLMPRLIEDVVP-DDRDDVDIILNT 410
Prot=RyR1 isform from fish	Query 246 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|\|\|\|\| \| ++\| \|\|\|\|\|\|\| \|++\|# \|\| \|\|+\|\|\| +\|\|\| \|\|\|\|\| \|\|\|\|\|\| Sbjct 1368 ENEKDPQQRKQEER-FLFKAKKPALIT#PPPVVPTMPRLVEDVVP-DDRDDVDIILNT 1422
Verminephrobacter eiseniae EF01-2	Query 263 FKAKKAAMMT#QPPATPTLPRLPHEVVPADDRD 294 \| \|+ +\|#\|\|\| \| \| \| \| \|\|\|\| Sbjct 637 FAARAVTNLT#QPPVAPATPEAPGAGAPDDDRD 668
Ashbya gossypii ATCC 10895	Query 246 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 302 \|+\|+\| \|++ \|+\| \| \| \|+\|\| \| # \| \| \|+ \|\|\| \| ++\|+ Sbjct 619 EDEEDRKTQELKRRRLLRKKKEAAAMV#PSPIQAT---DSDEITVADDESDAVSMVNS 672

* References

None.