* Structure Information
1. Primary Information
Length: 5035 aa
Average Mass: 565.332 kDa
Monoisotopic Mass: 564.971 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| Ins145_P3_rec 1. | 9 | 211 | 356.6 | 4.6e-104 |
| MIR 1. | 212 | 391 | 270.7 | 3.4e-78 |
| RYDR_ITPR 1. | 440 | 644 | 332.9 | 6.3e-97 |
| SPRY 1. | 659 | 797 | 135.5 | 1.7e-37 |
| RyR 1. | 850 | 944 | 222.5 | 1.1e-63 |
| RyR 2. | 964 | 1058 | 212.3 | 1.3e-60 |
| SPRY 2. | 1085 | 1208 | 160.5 | 4.9e-45 |
| --- cleavage 1400 --- |
| SPRY 3. | 1430 | 1570 | 153.8 | 5.2e-43 |
| RNA_polI_A34 1. | 1771 | 1926 | -77.2 | 5.7 |
| RIIa 1. | 1938 | 1975 | -8.8 | 6.8 |
| PRA-PH 1. | 2054 | 2127 | -46.7 | 2.8 |
| RYDR_ITPR 2. | 2158 | 2366 | 306.7 | 5e-89 |
| RyR 3. | 2735 | 2829 | 217.2 | 4.2e-62 |
| --- cleavage 2844 --- |
| RyR 4. | 2855 | 2943 | 184.4 | 3.2e-52 |
| Hpt 1. | 3091 | 3165 | -9.0 | 6 |
| Transposase_12 1. | 3327 | 3387 | -55.4 | 2.9 |
| XPA_C 1. | 3524 | 3569 | -8.6 | 9.7 |
| GvpG 1. | 3694 | 3770 | -24.4 | 3.6 |
| HTH_DeoR 1. | 3808 | 3857 | -17.5 | 5.4 |
| RIH_assoc 1. | 3866 | 3999 | 326.0 | 7.8e-95 |
| CHCH 1. | 4094 | 4133 | -5.0 | 6.1 |
| HR1 1. | 4183 | 4256 | -11.4 | 2.3 |
| CoatB 1. | 4266 | 4313 | -9.1 | 7.3 |
| RR_TM4-6 1. | 4378 | 4668 | 418.8 | 9e-123 |
| Ion_trans 1. | 4759 | 4934 | 36.2 | 1.4e-07 |
| zf-C2H2 1. | 4954 | 4981 | 1.3 | 8.8 |
3. Sequence Information
Fasta Sequence: XSB1620.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] TEKKKTRKIS2844-QSAQTYDARE
Ser2844 
Gln
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Thr2835 | Glu2836 | Lys2837 | Lys2838 | Lys2839 | Thr2840 | Arg2841 | Lys2842 | Ile2843 | Ser2844 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln2845 | Ser2846 | Ala2847 | Gln2848 | Thr2849 | Tyr2850 | Asp2851 | Ala2852 | Arg2853 | Glu2854 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
KAMIAWEWTIEKAREGEEEKTEKKKTRKISQSAQTYDAREGYNPQPPDLSGVTLSRELQA
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
82.80 |
6 |
R |
| 2 |
Sus scrofa |
82.80 |
4 |
ryanodine receptor |
| 3 |
Oryctolagus cuniculus |
79.70 |
2 |
RYR1_RABIT Ryanodine receptor 1 (Skeletal muscle-t |
| 4 |
Homo sapiens |
78.60 |
12 |
skeletal muscle ryanodine receptor isoform 2 |
| 5 |
Mus musculus |
75.90 |
4 |
ryanodine receptor 1, skeletal muscle |
| 6 |
Rattus norvegicus |
75.90 |
2 |
PREDICTED: similar to ryanodine receptor 1, skelet |
| 7 |
Bos taurus |
70.90 |
2 |
PREDICTED: similar to Ryanodine receptor 1 (Skelet |
| 8 |
Danio rerio |
61.20 |
2 |
PREDICTED: similar to ryanodine receptor RyR1 |
| 9 |
Tetraodon nigroviridis |
58.50 |
3 |
unnamed protein product |
| 10 |
Prot=RyR1 isform from fish |
54.30 |
1 |
- |
| 11 |
Macaca mulatta |
53.10 |
1 |
PREDICTED: similar to ryanodine receptor 1 (skelet |
| 12 |
Rana catesbeiana |
43.50 |
1 |
ryanodine receptor alpha isoform |
| 13 |
Hemicentrotus pulcherrimus |
40.40 |
1 |
ryanodine receptor |
| 14 |
Pan troglodytes |
39.30 |
1 |
PREDICTED: cardiac muscle ryanodine receptor |
| 15 |
Canis familiaris |
39.30 |
1 |
PREDICTED: similar to Ryanodine receptor 2 (Cardia |
| 16 |
Monodelphis domestica |
37.70 |
1 |
PREDICTED: similar to cardiac muscle ryanodine rec |
| 17 |
Gallus gallus |
37.70 |
1 |
PREDICTED: similar to cardiac muscle ryanodine rec |
| 18 |
Strongylocentrotus purpuratus |
32.70 |
1 |
PREDICTED: similar to ryanodine receptor, partial |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|||||||||| ||#||||||||||||||||||||||||||||||
Sbjct 2815 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|
| Sus scrofa |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|||||||||| ||#||||||||||||||||||||||||||||||
Sbjct 2815 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|
| Oryctolagus cuniculus |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|||||||||| ||#|+||||| ||||||||||||||||||||||
Sbjct 2814 KAMIAWEWTIEKAREGEEERTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSGVTLSRELQA 2873
|
| Homo sapiens |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|||||||||| ||#||||||| |||||||||||| |||||||||
Sbjct 2814 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDPREGYNPQPPDLSAVTLSRELQA 2873
|
| Mus musculus |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|||||||||+ ||#|+||||| |||||||||||| |||||||||
Sbjct 2815 KAMIAWEWTVEKAREGEEEKTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|
| Rattus norvegicus |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
|||||||||+ ||#|+||||| |||||||||||| |||||||||
Sbjct 2815 KAMIAWEWTVEKAREGEEEKTEKKKTRKIS#QTAQTYDPREGYNPQPPDLSVVTLSRELQA 2874
|
| Bos taurus |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVT 2867
|||||||||| ||#|||||||||||||||||||||||
Sbjct 966 KAMIAWEWTIEKAREGEEEKTEKKKTRKIS#QSAQTYDAREGYNPQPPDLSGVT 1018
|
| Danio rerio |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQ-TYDAREGYNPQPPDLSGVTLSRELQ 2873
|||||||||+ ||#|+|| ||| ||+||| |+||+|||||||
Sbjct 2852 KAMIAWEWTLDKARDGEDEKVEKKTARKIS#QTAQATYDPSHGYSPQPIDISGMTLSRELQ 2911
Query 2874 A 2874
+
Sbjct 2912 S 2912
|
| Tetraodon nigroviridis |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQ-TYDAREGYNPQPPDLSGVTLSRELQ 2873
|||+|||||+ ||#|+|| ||| ||+||| |+||+ ||||||
Sbjct 2341 KAMLAWEWTLEKARDGEGEVEKKAATRKIS#QTAQATYDPSHGYSPQPIDISGMALSRELQ 2400
Query 2874 A 2874
+
Sbjct 2401 S 2401
|
| Prot=RyR1 isform from fish |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQ-TYDAREGYNPQPPDLSGVTLSRELQ 2873
|||+|||| | ||#|+|| ||| ||+||| |+| | |||+||
Sbjct 2853 KAMLAWEWNIEKAREEEESEKKKATSRKIS#QTAQATYDPSHGYSPQPIDISHVALSRDLQ 2912
Query 2874 A 2874
+
Sbjct 2913 S 2913
|
| Macaca mulatta |
Query 2846 SAQTYDAREGYNPQPPDLSGVTLSRELQ 2873
| +||| |||||||||||| ||||||||
Sbjct 818 SEKTYDPREGYNPQPPDLSAVTLSRELQ 845
|
| Rana catesbeiana |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXX-IS#QSAQ-TYDAR-EGYNPQPPDLSGVTLSRE 2871
|||||||| + ||#|+|| ||| + ++| | ||+|+|||||
Sbjct 2800 KAMIAWEWLVEKAREGEEDRTEKKTKTRKIS#QTAQQTYDPMVQTFSPTPIDLTGLTLSRE 2859
Query 2872 LQA 2874
||+
Sbjct 2860 LQS 2862
|
| Hemicentrotus pulcherrimus |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQ 2873
||++|| |++ # | ++ ||||+| |+| +||+||+|
Sbjct 2985 KALLAWGWSVERSKASEQQSQAHRPRRLSK#ASLGAMESPHGYNPRPIDVSNITLTREMQ 3043
|
| Pan troglodytes |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
| |+|| | | # | + || ||+|+ |+| |||||+| |
Sbjct 2826 KTMLAWGWRIERTREGDSMALYNRTRRISQ#TSQVSVDAAHGYSPRAIDMSNVTLSRDLHA 2885
|
| Canis familiaris |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
| |+|| | | # | + || ||+|+ |+| |||||+| |
Sbjct 2774 KTMLAWGWRIERTREGDSMALYNRTRRISQ#TSQVSVDAAHGYSPRAIDMSNVTLSRDLHA 2833
|
| Monodelphis domestica |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
| |+|| | | # | + | ||+|+ |+| |||||+| |
Sbjct 2850 KTMLAWGWRIERTREGDSMALYTRTRRISQ#TSQVSIDTAHGYSPRAIDMSNVTLSRDLHA 2909
|
| Gallus gallus |
Query 2815 KAMIAWEWTIXXXXXXXXXXXXXXXXXXIS#QSAQTYDAREGYNPQPPDLSGVTLSRELQA 2874
| |+|| | | # | + || ||+|+ |+| |||||++ |
Sbjct 614 KTMLAWGWRIERTREGDSMALYNRTRRISQ#TSQISVDAAHGYSPRAIDMSNVTLSRDVHA 673
|
| Strongylocentrotus purpuratus |
Query 2851 DAREGYNPQPPDLSGVTLSRELQ 2873
++ ||||+| |+| +||+||+|
Sbjct 4 ESPHGYNPRPIDVSNITLTREMQ 26
|
[Site 2] FKAKKAAMMT1400-QPPATPTLPR
Thr1400 Gln
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Phe1391 | Lys1392 | Ala1393 | Lys1394 | Lys1395 | Ala1396 | Ala1397 | Met1398 | Met1399 | Thr1400 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln1401 | Pro1402 | Pro1403 | Ala1404 | Thr1405 | Pro1406 | Thr1407 | Leu1408 | Pro1409 | Arg1410 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
PRAENEKDATTEKNKKRGFLFKAKKAAMMTQPPATPTLPRLPHEVVPADDRDDPDIILNT
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
126.00 |
4 |
R |
| 2 |
Sus scrofa |
126.00 |
4 |
ryanodine receptor |
| 3 |
Homo sapiens |
117.00 |
6 |
skeletal muscle ryanodine receptor isoform 2 |
| 4 |
Oryctolagus cuniculus |
117.00 |
1 |
RYR1_RABIT Ryanodine receptor 1 (Skeletal muscle-t |
| 5 |
Rattus norvegicus |
111.00 |
2 |
PREDICTED: similar to ryanodine receptor 1, skelet |
| 6 |
Mus musculus |
111.00 |
2 |
ryanodine receptor 1, skeletal muscle |
| 7 |
Monodelphis domestica |
105.00 |
1 |
PREDICTED: hypothetical protein |
| 8 |
Tetraodon nigroviridis |
87.00 |
2 |
unnamed protein product |
| 9 |
Rana catesbeiana |
84.00 |
1 |
ryanodine receptor alpha isoform |
| 10 |
Danio rerio |
81.60 |
2 |
PREDICTED: hypothetical protein, partial |
| 11 |
Prot=RyR1 isform from fish |
70.90 |
1 |
- |
| 12 |
Verminephrobacter eiseniae EF01-2 |
33.50 |
1 |
outer membrane protein |
| 13 |
Ashbya gossypii ATCC 10895 |
32.30 |
1 |
AFR489Wp |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 1371 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1371 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|
| Sus scrofa |
Query 1371 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1371 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|
| Homo sapiens |
Query 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|||||||||||||||||||||||| ||||#|||||||||||||+|||||+||||+|||||
Sbjct 1371 RAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPATPTLPRLPHDVVPADNRDDPEIILNT 1429
|
| Oryctolagus cuniculus |
Query 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|||||||||||||||||||||||||||||#|||||| ||||||+|||||+||||+|||||
Sbjct 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPALPRLPHDVVPADNRDDPEIILNT 1430
|
| Rattus norvegicus |
Query 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|||||||||||||||||||||||| ||||#|||+|| ||||| +|||||+||||+|||||
Sbjct 1373 RAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|
| Mus musculus |
Query 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|||||||||||||||||||||||| ||||#|||+|| ||||| +|||||+||||+|||||
Sbjct 1373 RAENEKDATTEKNKKRGFLFKAKKVAMMT#QPPSTPALPRLPRDVVPADNRDDPEIILNT 1431
|
| Monodelphis domestica |
Query 1372 RAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|||||||+ |||+||||||||||||||||#| |||| ||||| +|||||+||||+|||||
Sbjct 1299 RAENEKDSATEKSKKRGFLFKAKKAAMMT#QAPATPALPRLPRDVVPADNRDDPEIILNT 1357
|
| Tetraodon nigroviridis |
Query 1374 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
||||||+|||+||+||||||||||++ # || ||+||| +||| ||||| ||||||
Sbjct 1329 ENEKDASTEKSKKKGFLFKAKKAALIN#PPPVVPTMPRLVEDVVP-DDRDDVDIILNT 1384
|
| Rana catesbeiana |
Query 1371 PRAENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|+ ||||| |||| |||||||| || | ++# | ||+||| |||| ||||||+|| ||
Sbjct 1361 PKPENEKDTTTEKTKKRGFLFKPKKPAFIS#TTPVVPTMPRLMKEVVP-DDRDDPEIIRNT 1419
|
| Danio rerio |
Query 1374 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
||+|| +|| ||+|||||||||||+|# || | +||| +||| ||||| ||||||
Sbjct 355 ENDKDTASEKGKKKGFLFKAKKAAMIT#PPPPPPLMPRLIEDVVP-DDRDDVDIILNT 410
|
| Prot=RyR1 isform from fish |
Query 1374 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
||||| | ++| ||||||| |++|# || ||+||| +||| ||||| ||||||
Sbjct 1368 ENEKDPQQRKQEER-FLFKAKKPALIT#PPPVVPTMPRLVEDVVP-DDRDDVDIILNT 1422
|
| Verminephrobacter eiseniae EF01-2 |
Query 1391 FKAKKAAMMT#QPPATPTLPRLPHEVVPADDRD 1422
| |+ +|#||| | | | | ||||
Sbjct 637 FAARAVTNLT#QPPVAPATPEAPGAGAPDDDRD 668
|
| Ashbya gossypii ATCC 10895 |
Query 1374 ENEKDATTEKNKKRGFLFKAKKAAMMT#QPPATPTLPRLPHEVVPADDRDDPDIILNT 1430
|+|+| |++ |+| | | |+|| | # | | |+ ||| | ++|+
Sbjct 619 EDEEDRKTQELKRRRLLRKKKEAAAMV#PSPIQAT---DSDEITVADDESDAVSMVNS 672
|
* References
[PubMed ID: 1329581] Harbitz I, Kristensen T, Bosnes M, Kran S, Davies W, DNA sequence of the skeletal muscle calcium release channel cDNA and verification of the Arg615----Cys615 mutation, associated with porcine malignant hyperthermia, in Norwegian landrace pigs. Anim Genet. 1992;23(5):395-402.
» More (2) « Hide Below
[PubMed ID: 8288238] ... Leeb T, Schmolzl S, Brem G, Brenig B, Genomic organization of the porcine skeletal muscle ryanodine receptor (RYR1) gene coding region 4624 to 7929. Genomics. 1993 Nov;18(2):349-54.
[PubMed ID: 2174405] ... Harbitz I, Chowdhary B, Thomsen PD, Davies W, Kaufmann U, Kran S, Gustavsson I, Christensen K, Hauge JG, Assignment of the porcine calcium release channel gene, a candidate for the malignant hyperthermia locus, to the 6p11----q21 segment of chromosome 6. Genomics. 1990 Oct;8(2):243-8.
