XSB1644 : Calpain 2, large [catalytic] subunit precursor variant [Homo sapiens]
[ CaMP Format ]
This entry is computationally expanded from SB0001
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | Calpain 2, large [catalytic] subunit variant; Calpain 2; large [catalytic] subunit precursor variant |
| Gene Names | Not available |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 729 aa
Average Mass: 83.109 kDa
Monoisotopic Mass: 83.056 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| --- cleavage 48 --- | ||||
| --- cleavage 38 --- | ||||
| Peptidase_C2 1. | 74 | 373 | 800.6 | 1e-237 |
| Calpain_III 1. | 384 | 539 | 393.6 | 3.4e-115 |
| efhand 1. | 605 | 633 | 20.4 | 0.0074 |
| efhand 2. | 635 | 663 | 19.4 | 0.015 |
| efhand 3. | 700 | 728 | 4.0 | 7.6 |
3. Sequence Information
Fasta Sequence: XSB1644.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Cleavage sites (±10aa)
[Site 1] KDREAAEGLG48-SHERAIKYLN
Gly48 
Ser
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Lys39 | Asp40 | Arg41 | Glu42 | Ala43 | Ala44 | Glu45 | Gly46 | Leu47 | Gly48 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ser49 | His50 | Glu51 | Arg52 | Ala53 | Ile54 | Lys55 | Tyr56 | Leu57 | Asn58 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| FSAQYGRRDRSMAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDP |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | catalytic | 122.00 | 1 | Calpain 2, large |
| 2 | Homo sapiens | 100.00 | 25 | AF261089_1 calpain large polypeptide L2 |
| 3 | N/A | 100.00 | 14 | C |
| 4 | Macaca mulatta | 99.40 | 3 | PREDICTED: calpain 2, large subunit |
| 5 | Mus musculus | 95.50 | 19 | unnamed protein product |
| 6 | Rattus norvegicus | 95.50 | 9 | calpain 2 |
| 7 | Bos taurus | 94.70 | 7 | PREDICTED: similar to Calpain 2, (m/II) large subu |
| 8 | Monodelphis domestica | 80.10 | 2 | PREDICTED: similar to calpain 2 |
| 9 | Gallus gallus | 74.30 | 6 | calpain 1, (mu/I) large subunit |
| 10 | Xenopus laevis | 71.60 | 10 | hypothetical protein LOC399075 |
| 11 | Xenopus tropicalis | 71.60 | 6 | calpain 2, (m/II) large subunit |
| 12 | Canis familiaris | 70.50 | 4 | PREDICTED: similar to calpain 8 |
| 13 | Coturnix coturnix | 70.10 | 1 | quail calpain |
| 14 | Danio rerio | 66.20 | 27 | hypothetical protein LOC550505 |
| 15 | Stizostedion vitreum vitreum | 63.20 | 1 | putative calpain-like protein |
| 16 | Tetraodon nigroviridis | 62.00 | 6 | unnamed protein product |
| 17 | Oncorhynchus mykiss | 62.00 | 3 | m-calpain |
| 18 | Pan troglodytes | 59.70 | 6 | PREDICTED: hypothetical protein isoform 9 |
| 19 | Pongo pygmaeus | 59.70 | 1 | CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 20 | synthetic construct | 59.70 | 1 | Homo sapiens calpain 1, (mu/I) large subunit |
| 21 | Sus scrofa | 57.00 | 3 | micromolar calcium-activated neutral protease 1 is |
| 22 | Macaca fascicularis | 43.10 | 1 | unnamed protein product |
| 23 | Aedes aegypti | 35.00 | 2 | calpain, putative |
| 24 | Oryctolagus cuniculus | 34.30 | 1 | calpain 3, (p94) |
| 25 | Bos grunniens | 33.90 | 1 | micromolar calcium-activated neutral protease 1 la |
| 26 | Drosophila melanogaster | 33.10 | 6 | CAA55297.1 |
| 27 | Anopheles gambiae str. PEST | 33.10 | 1 | ENSANGP00000028181 |
Top-ranked sequences
| organism | matching |
|---|---|
| catalytic | Query 19 FSAQYGRRDRSMAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 19 FSAQYGRRDRSMAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |
| Homo sapiens | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |||||||||||||||||||#|||||||||||||||||||||||||||||| Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |
| N/A | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |||||||||||||||||||#|||||||||||||||||||||||||||||| Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |
| Macaca mulatta | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |||||||| ||||||||||#|||||||||||||||||||||||||||||| Sbjct 1 MAGIAAKLVKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 |
| Mus musculus | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 ||||| |||||||||||||#|||||||||||||| ||||||||| ||||| Sbjct 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 |
| Rattus norvegicus | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 ||||| |||||||||||||#|||||||||||||| ||||||||| ||||| Sbjct 1 MAGIAMKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 |
| Bos taurus | Query 29 SMAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 +|||||||||||||||||||#|||||+||||||| |||+|||||| ||||| Sbjct 176 TMAGIAAKLAKDREAAEGLG#SHERAVKYLNQDYAALRDECLEAGALFQDP 225 |
| Monodelphis domestica | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |+|||||+ +||||| |||#+||+|+|||||||| | +|||| | ||||| Sbjct 1 MSGIAAKVVRDREAAAGLG#THEQAVKYLNQDYETLLSECLEEGKLFQDP 49 |
| Gallus gallus | Query 32 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 ||||+| +|| |||+|# | |+||||||||||+ ||+|+||||+|| Sbjct 6 GIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQECIESGTLFRDP 52 |
| Xenopus laevis | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |+|+| +|||+| | | |#++|+|+ |||||+||||||||++|+||+|| Sbjct 1 MSGVADRLAKERARASGFG#TNEKALPYLNQDFEALRNECLQSGSLFKDP 49 |
| Xenopus tropicalis | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |+|+| +|||+| | |||#++|+|+ ||+|||||||||||++||||+| Sbjct 1 MSGVADRLAKERALASGLG#TNEKALPYLSQDYEALRNECLQSGTLFKD 48 |
| Canis familiaris | Query 24 GRRDRSMAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 | |+ +||++|| + || |||#||++|+||| ||+| || +||+|| ||+|| Sbjct 88 GSEPTDMSALAARIAKQQAAAGGLG#SHQKAVKYLGQDFEKLRQQCLDAGVLFKDP 142 |
| Coturnix coturnix | Query 32 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 ||||+| +|| |||+|# | |+||||||||||+ |+|+| ||+|| Sbjct 6 GIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQACIESGALFRDP 52 |
| Danio rerio | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |+||| || |+| | |+|#++ +| |+||||||||+ ||||+| |||| Sbjct 1 MSGIATKLQKNRARAAGIG#TNAQAAKFLNQDYEALKRECLESGRLFQD 48 |
| Stizostedion vitreum vitreum | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |+|||| | + || +|+|#| +|+||||||||||| |||+ +||+| Sbjct 1 MSGIAATLQRRREKEQGIG#SCSQAVKYLNQDYEALRRRCLESESLFKD 48 |
| Tetraodon nigroviridis | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |+|||||| |+ |+| |#+ +| ||||||+|+|| ||| | |||| Sbjct 1 MSGIAAKLQHHRDRAQGFG#TASQAQKYLNQDFESLRESCLERGELFQD 48 |
| Oncorhynchus mykiss | Query 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 |+|+|+ ||| | | | |#++ |+||||||+|||| +|| | || || Sbjct 1 MSGMASNLAKKRALAAGFG#TNANAVKYLNQDFEALRAQCLNRGGLFSDP 49 |
| Pan troglodytes | Query 32 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |++|++ | | |||# || ||||| |||| || ||++||||+| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58 |
| Pongo pygmaeus | Query 32 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |++|++ | | |||# || ||||| |||| || ||++||||+| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58 |
| synthetic construct | Query 32 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |++|++ | | |||# || ||||| |||| || ||++||||+| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58 |
| Sus scrofa | Query 32 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 |++|++ | | |||# || ||||| |||| || ||++|+||+| Sbjct 13 GVSAQVQKLRAKELGLG#RHENAIKYLGQDYEQLRAHCLQSGSLFRD 58 |
| Macaca fascicularis | Query 21 AQYGRRDRSMAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 + | + |+ | + | | |+|# | | + || +| || || | ||+|| Sbjct 25 SSYAEPTYTDTGMVAHINYSRLKASGVG#QHHNAHNFGNQSFEELRAVCLRKGELFEDP 82 |
| Aedes aegypti | Query 59 QDYEALRNECLEAGTLFQDP 78 ||| ||++|||+|+||+|| Sbjct 115 QDYYVLRSQCLESGSLFEDP 134 |
| Oryctolagus cuniculus | Query 36 KLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 77 ++ ++|+ | |#+ + ||| ||+ || || ||+| Sbjct 12 RVIRERDRRNGEG#TVTQPIKYEGQDFVVLRQRCLAQKCLFED 53 |
| Bos grunniens | Query 56 YLNQDYEALRNECLEAGTLFQD 77 || |||| || ||+ | ||+| Sbjct 1 YLGQDYEQLRVHCLQRGALFRD 22 |
| Drosophila melanogaster | Query 59 QDYEALRNECLEAGTLFQDP 78 |||| + | || +|+||+|| Sbjct 50 QDYETILNSCLASGSLFEDP 69 |
| Anopheles gambiae str. PEST | Query 27 DRSMAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78 ||| + ||++ | + | #+ | +|++|||+ || ||||+|| Sbjct 18 DRSSSSIASE--SDCRSNTDLD#AFENQPTV--KDFQALRDRCLAEGTLFEDP 65 |
[Site 2] SMAGIAAKLA38-KDREAAEGLG
Ala38 Lys
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ser29 | Met30 | Ala31 | Gly32 | Ile33 | Ala34 | Ala35 | Lys36 | Leu37 | Ala38 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Lys39 | Asp40 | Arg41 | Glu42 | Ala43 | Ala44 | Glu45 | Gly46 | Leu47 | Gly48 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| AERSAESPRPFSAQYGRRDRSMAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNEC |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | catalytic | 122.00 | 1 | Calpain 2, large |
| 2 | Homo sapiens | 80.90 | 20 | AF261089_1 calpain large polypeptide L2 |
| 3 | N/A | 80.90 | 12 | C |
| 4 | Macaca mulatta | 79.30 | 3 | PREDICTED: calpain 2, large subunit |
| 5 | Mus musculus | 77.40 | 15 | unnamed protein product |
| 6 | Rattus norvegicus | 77.40 | 5 | calpain 2 |
| 7 | Bos taurus | 76.60 | 6 | PREDICTED: similar to Calpain 2, (m/II) large subu |
| 8 | Monodelphis domestica | 64.30 | 3 | PREDICTED: similar to calpain 2 |
| 9 | Xenopus tropicalis | 58.20 | 5 | calpain 2, (m/II) large subunit |
| 10 | Gallus gallus | 58.20 | 5 | PREDICTED: similar to calpain 8 (nCL-2) |
| 11 | Xenopus laevis | 57.00 | 9 | hypothetical protein LOC399075 |
| 12 | Coturnix coturnix | 55.50 | 1 | quail calpain |
| 13 | Canis familiaris | 55.10 | 4 | PREDICTED: similar to calpain 8 |
| 14 | Stizostedion vitreum vitreum | 53.10 | 1 | putative calpain-like protein |
| 15 | Danio rerio | 52.40 | 23 | hypothetical protein LOC550505 |
| 16 | Oncorhynchus mykiss | 51.20 | 3 | calpain 2 catalytic subunit |
| 17 | Tetraodon nigroviridis | 48.90 | 6 | unnamed protein product |
| 18 | Pan troglodytes | 46.20 | 5 | PREDICTED: hypothetical protein isoform 9 |
| 19 | Pongo pygmaeus | 46.20 | 1 | CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 20 | synthetic construct | 46.20 | 1 | Homo sapiens calpain 1, (mu/I) large subunit |
| 21 | Sus scrofa | 45.10 | 2 | micromolar calcium-activated neutral protease 1 is |
Top-ranked sequences
| organism | matching |
|---|---|
| catalytic | Query 9 AERSAESPRPFSAQYGRRDRSMAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 9 AERSAESPRPFSAQYGRRDRSMAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |
| Homo sapiens | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |||||||||#|||||||||||||||||||||||||||||| Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |
| N/A | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |||||||||#|||||||||||||||||||||||||||||| Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |
| Macaca mulatta | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |||||||| #|||||||||||||||||||||||||||||| Sbjct 1 MAGIAAKLV#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 |
| Mus musculus | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 ||||| |||#|||||||||||||||||||||||| ||||| Sbjct 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 |
| Rattus norvegicus | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 ||||| |||#|||||||||||||||||||||||| ||||| Sbjct 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 |
| Bos taurus | Query 29 SMAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 +|||||||||#|||||||||||||||+||||||| |||+|| Sbjct 176 TMAGIAAKLA#KDREAAEGLGSHERAVKYLNQDYAALRDEC 215 |
| Monodelphis domestica | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |+|||||+ #+||||| |||+||+|+|||||||| | +|| Sbjct 1 MSGIAAKVV#RDREAAAGLGTHEQAVKYLNQDYETLLSEC 39 |
| Xenopus tropicalis | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |+|+| +||#|+| | |||++|+|+ ||+|||||||||| Sbjct 1 MSGVADRLA#KERALASGLGTNEKALPYLSQDYEALRNEC 39 |
| Gallus gallus | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |||+||+| #++| |||||||+ +|| || ||||||| | Sbjct 1 MAGLAARLC#QERAAAEGLGSYRKAISYLGQDYEALRQRC 39 |
| Xenopus laevis | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |+|+| +||#|+| | | |++|+|+ |||||+||||||| Sbjct 1 MSGVADRLA#KERARASGFGTNEKALPYLNQDFEALRNEC 39 |
| Coturnix coturnix | Query 32 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 ||||+| #+|| |||+| | |+||||||||||+ | Sbjct 6 GIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQAC 42 |
| Canis familiaris | Query 24 GRRDRSMAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 | |+ +||++|#| + || |||||++|+||| ||+| || +| Sbjct 88 GSEPTDMSALAARIA#KQQAAAGGLGSHQKAVKYLGQDFEKLRQQC 132 |
| Stizostedion vitreum vitreum | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |+|||| | #+ || +|+|| +|+||||||||||| | Sbjct 1 MSGIAATLQ#RRREKEQGIGSCSQAVKYLNQDYEALRRRC 39 |
| Danio rerio | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |+||| || #|+| | |+|++ +| |+||||||||+ || Sbjct 1 MSGIATKLQ#KNRARAAGIGTNAQAAKFLNQDYEALKREC 39 |
| Oncorhynchus mykiss | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |||+|+ ||#| | | | |++ | ||||||+|+||+|| Sbjct 1 MAGVASTLA#KKRALAAGFGTNANASKYLNQDFESLRSEC 39 |
| Tetraodon nigroviridis | Query 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |+|||||| # |+ |+| |+ +| ||||||+|+|| | Sbjct 1 MSGIAAKLQ#HHRDRAQGFGTASQAQKYLNQDFESLRESC 39 |
| Pan troglodytes | Query 32 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |++|++ #| | ||| || ||||| |||| || | Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49 |
| Pongo pygmaeus | Query 32 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |++|++ #| | ||| || ||||| |||| || | Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49 |
| synthetic construct | Query 32 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |++|++ #| | ||| || ||||| |||| || | Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49 |
| Sus scrofa | Query 32 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68 |++|++ #| | ||| || ||||| |||| || | Sbjct 13 GVSAQVQ#KLRAKELGLGRHENAIKYLGQDYEQLRAHC 49 |
* References
None.