CaMPDB :: Substrate

XSB1651 : m-calpain [Mus musculus]

This entry is computationally expanded from SB0001

* Basic Information

Organism	Mus musculus (house mouse)
Protein Names	Calpain-2 catalytic subunit; 3.4.22.53; Calpain-2 large subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; M-calpain; Millimolar-calpain; 80 kDa M-calpain subunit; CALP80
Gene Names	Capn2
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
CAA71227	N/A	N/A	O08529	N/A	N/A	N/A	mmu:12334

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 700 aa

Average Mass: 79.830 kDa

Monoisotopic Mass: 79.780 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 19 ---
--- cleavage 9 ---
Peptidase_C2 1.	45	344	807.0	1.2e-239
Calpain_III 1.	355	510	380.1	4e-111
efhand 1.	576	604	20.4	0.0074
efhand 2.	606	634	19.4	0.015
efhand 3.	671	698	1.8	13

3. Sequence Information

Fasta Sequence: XSB1651.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites]

Cleavage sites (±10aa)

[Site 1] KDREAAEGLG¹⁹-SHERAIKYLN

Gly¹⁹ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser²⁰	His²¹	Glu²²	Arg²³	Ala²⁴	Ile²⁵	Lys²⁶	Tyr²⁷	Leu²⁸	Asn²⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDP

Summary

#	organism	max score	hits	top seq
1	Mus musculus	100.00	20	unnamed protein product
2	N/A	100.00	14	C
3	Rattus norvegicus	99.80	9	calpain 2
4	Homo sapiens	95.50	25	calpain 2, large subunit
5	catalytic	95.50	1	Calpain 2, large
6	Macaca mulatta	94.00	3	PREDICTED: calpain 2, large subunit
7	Bos taurus	92.40	7	Unknown (protein for MGC:138930)
8	Monodelphis domestica	80.10	2	PREDICTED: similar to calpain 2
9	Xenopus laevis	69.70	10	hypothetical protein LOC399075
10	Gallus gallus	68.90	5	calpain 1, (mu/I) large subunit
11	Coturnix coturnix	68.20	1	quail calpain
12	Xenopus tropicalis	67.80	6	calpain 2, (m/II) large subunit
13	Canis familiaris	67.40	6	PREDICTED: similar to calpain 8
14	Danio rerio	64.30	31	hypothetical protein LOC550505
15	Tetraodon nigroviridis	60.10	7	unnamed protein product
16	Oncorhynchus mykiss	60.10	3	m-calpain
17	Pan troglodytes	59.70	6	PREDICTED: similar to calpain, partial
18	Stizostedion vitreum vitreum	59.70	1	putative calpain-like protein
19	Pongo pygmaeus	55.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	synthetic construct	55.80	1	Homo sapiens calpain 1, (mu/I) large subunit
21	Sus scrofa	55.10	3	micromolar calcium-activated neutral protease 1 is
22	Macaca fascicularis	40.80	1	unnamed protein product
23	Bos grunniens	35.40	1	micromolar calcium-activated neutral protease 1 la
24	Drosophila melanogaster	35.00	6	CAA55297.1
25	Aedes aegypti	35.00	1	calpain, putative
26	Oryctolagus cuniculus	34.70	1	calpain 3, (p94)

Top-ranked sequences

organism	matching
Mus musculus	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
N/A	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
Rattus norvegicus	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAMKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
Homo sapiens	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
catalytic	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 30 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 78
Macaca mulatta	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\| \|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLVKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Bos taurus	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLAKDREAAEGLG#SHERAVKYLNQDYAALRDECLEAGALFQDP 49
Monodelphis domestica	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|+\|\|\| \|+ +\|\|\|\|\| \|\|\|#+\|\|+\|+\|\|\|\|\|\|\|\|\|\| +\|\|\|\| \| \|\|\|\|\| Sbjct 1 MSGIAAKVVRDREAAAGLG#THEQAVKYLNQDYETLLSECLEEGKLFQDP 49
Xenopus laevis	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|+\|+\| +\|\|\|+\| \| \| \|#++\|+\|+ \|\|\|\|\|+\| \|\|\|\|\|\|++\|+\|\|+\|\| Sbjct 1 MSGVADRLAKERARASGFG#TNEKALPYLNQDFEALRNECLQSGSLFKDP 49
Gallus gallus	Query 3 GIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\| +\| +\|\| \|\|\|+\|# \| \|+\|\|\|\|\|\|\|\| \|+ \|\|+\|+\| \|\|+\|\| Sbjct 6 GIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQECIESGTLFRDP 52
Coturnix coturnix	Query 3 GIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\|\| +\| +\|\| \|\|\|+\|# \| \|+\|\|\|\|\|\|\|\| \|+ \|+\|+\|\|\|\|+\|\| Sbjct 6 GIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQACIESGALFRDP 52
Xenopus tropicalis	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|+\|+\| +\|\|\|+\| \| \|\|\|#++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\|\|++\| \|\|+\| Sbjct 1 MSGVADRLAKERALASGLG#TNEKALPYLSQDYEALRNECLQSGTLFKD 48
Canis familiaris	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|+ +\| ++\|\| + \|\| \|\|\|#\|\|++\|+\|\|\| \|\|+\| \|\| +\|\|+\|\| \|\|+\|\| Sbjct 94 MSALAARIAKQQAAAGGLG#SHQKAVKYLGQDFEKLRQQCLDAGVLFKDP 142
Danio rerio	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|+\|\|\| \|\| \|+\| \| \|+\|#++ +\| \|+\|\|\|\|\|\| \|+ \|\|\|\|+\| \|\|\|\| Sbjct 1 MSGIATKLQKNRARAAGIG#TNAQAAKFLNQDYEALKRECLESGRLFQD 48
Tetraodon nigroviridis	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|+\|\|\| \|\| \|+ \|+\| \|#+ +\| \|\|\|\|\|\|+\|+\|\| \|\|\| \| \|\|\|\| Sbjct 1 MSGIAAKLQHHRDRAQGFG#TASQAQKYLNQDFESLRESCLERGELFQD 48
Oncorhynchus mykiss	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|+\|+\| \|\|\| \| \| \| \|#++ \|+\|\|\|\|\|\|+\| \|\| +\|\| \| \|\| \|\| Sbjct 1 MSGMASNLAKKRALAAGFG#TNANAVKYLNQDFEALRAQCLNRGGLFSDP 49
Pan troglodytes	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|\| \| +++ \| \| +\|\|\|#\|++ \|+\|\|\| \|\|++\|\|\| +\|\|++\| \|\|+\|\| Sbjct 1 MAAQAAGVSRQRAATQGLG#SNQNALKYLGQDFKTLRQQCLDSGVLFKDP 49
Stizostedion vitreum vitreum	Query 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|+\|\|\| \| + \|\| +\|+\|#\| +\|+\|\|\|\|\|\|\|\| \|\| \|\|\|+ +\|\|+\| Sbjct 1 MSGIAATLQRRREKEQGIG#SCSQAVKYLNQDYEALRRRCLESESLFKD 48
Pongo pygmaeus	Query 3 GIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|++ ++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| \|\|+\| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
synthetic construct	Query 3 GIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|++ ++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\| \|\|+\| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
Sus scrofa	Query 3 GIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 \|++ ++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\|+\|\|+\| Sbjct 13 GVSAQVQKLRAKELGLG#RHENAIKYLGQDYEQLRAHCLQSGSLFRD 58
Macaca fascicularis	Query 3 GIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49 \|+ + \| \| \|+\|# \| \| + \|\| +\| \|\| \|\| \| \|\|+\|\| Sbjct 36 GMVAHINYSRLKASGVG#QHHNAHNFGNQSFEELRAVCLRKGELFEDP 82
Bos grunniens	Query 27 YLNQDYETLRNECLEAGALFQD 48 \|\| \|\|\|\| \|\| \|\|+ \|\|\|\|+\| Sbjct 1 YLGQDYEQLRVHCLQRGALFRD 22
Drosophila melanogaster	Query 30 QDYETLRNECLEAGALFQDP 49 \|\|\|\|\|+ \| \|\| +\|+\|\|+\|\| Sbjct 50 QDYETILNSCLASGSLFEDP 69
Aedes aegypti	Query 30 QDYETLRNECLEAGALFQDP 49 \|\|\| \|\|++\|\|\|+\|+\|\|+\|\| Sbjct 115 QDYYVLRSQCLESGSLFEDP 134
Oryctolagus cuniculus	Query 7 KLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQD 48 ++ ++\|+ \| \|#+ + \|\|\| \|\|+ \|\| \|\| \|\|+\| Sbjct 12 RVIRERDRRNGEG#TVTQPIKYEGQDFVVLRQRCLAQKCLFED 53

[Site 2] MAGIAIKLA⁹-KDREAAEGLG

Ala⁹ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	Met¹	Ala²	Gly³	Ile⁴	Ala⁵	Ile⁶	Lys⁷	Leu⁸	Ala⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNEC

Summary

#	organism	max score	hits	top seq
1	Mus musculus	81.30	15	unnamed protein product
2	N/A	81.30	12	C
3	Rattus norvegicus	80.10	5	calpain 2
4	Homo sapiens	77.40	17	AF261089_1 calpain large polypeptide L2
5	catalytic	77.40	1	Calpain 2, large
6	Macaca mulatta	75.90	3	PREDICTED: calpain 2, large subunit
7	Bos taurus	72.80	6	Unknown (protein for MGC:138930)
8	Monodelphis domestica	64.30	2	PREDICTED: similar to calpain 2
9	Xenopus tropicalis	56.20	5	calpain 2, (m/II) large subunit
10	Xenopus laevis	55.10	9	hypothetical protein LOC399075
11	Gallus gallus	54.70	5	PREDICTED: similar to calpain 8 (nCL-2)
12	Canis familiaris	52.00	3	PREDICTED: similar to calpain 8
13	Coturnix coturnix	52.00	1	quail calpain
14	Danio rerio	50.80	23	hypothetical protein LOC445091
15	Oncorhynchus mykiss	50.10	3	calpain 2 catalytic subunit
16	Stizostedion vitreum vitreum	49.70	1	putative calpain-like protein
17	Tetraodon nigroviridis	47.00	6	unnamed protein product
18	Pan troglodytes	45.40	5	PREDICTED: similar to calpain, partial
19	Pongo pygmaeus	44.30	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	synthetic construct	44.30	1	Homo sapiens calpain 1, (mu/I) large subunit
21	Sus scrofa	43.10	2	micromolar calcium-activated neutral protease 1 is

Top-ranked sequences

organism	matching
Mus musculus	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
N/A	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Rattus norvegicus	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\|+\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Homo sapiens	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
catalytic	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 30 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 68
Macaca mulatta	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAAKLV#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Bos taurus	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\| \|\|+\|\| Sbjct 1 MAGIAAKLA#KDREAAEGLGSHERAVKYLNQDYAALRDEC 39
Monodelphis domestica	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\| \|+ #+\|\|\|\|\| \|\|\|+\|\|+\|+\|\|\|\|\|\|\|\|\|\| +\|\| Sbjct 1 MSGIAAKVV#RDREAAAGLGTHEQAVKYLNQDYETLLSEC 39
Xenopus tropicalis	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|+\| +\|\|#\|+\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\| \|\|\|\|\| Sbjct 1 MSGVADRLA#KERALASGLGTNEKALPYLSQDYEALRNEC 39
Xenopus laevis	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|+\| +\|\|#\|+\| \| \| \|++\|+\|+ \|\|\|\|\|+\| \|\|\|\|\| Sbjct 1 MSGVADRLA#KERARASGFGTNEKALPYLNQDFEALRNEC 39
Gallus gallus	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|+\| +\| #++\| \|\|\|\|\|\|\|+ +\|\| \|\| \|\|\|\| \|\| \| Sbjct 1 MAGLAARLC#QERAAAEGLGSYRKAISYLGQDYEALRQRC 39
Canis familiaris	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+ +\| ++\|#\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\| Sbjct 94 MSALAARIA#KQQAAAGGLGSHQKAVKYLGQDFEKLRQQC 132
Coturnix coturnix	Query 3 GIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\| +\| #+\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\| \|+ \| Sbjct 6 GIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQAC 42
Danio rerio	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\|\|\|\| # \| \| \|+\|++ +\|+\|+\|\|\|\|\|\| \|+ \|\| Sbjct 1 MSGIAIKLQ#HKRAQAAGIGTNAQAVKFLNQDYEALKREC 39
Oncorhynchus mykiss	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\|\|+\| \|\|#\| \| \| \| \|++ \| \|\|\|\|\|\|+\|+\|\|+\|\| Sbjct 1 MAGVASTLA#KKRALAAGFGTNANASKYLNQDFESLRSEC 39
Stizostedion vitreum vitreum	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\| \| #+ \|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\| \|\| \| Sbjct 1 MSGIAATLQ#RRREKEQGIGSCSQAVKYLNQDYEALRRRC 39
Tetraodon nigroviridis	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|+\|\|\| \|\| # \|+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \| Sbjct 1 MSGIAAKLQ#HHRDRAQGFGTASQAQKYLNQDFESLRESC 39
Pan troglodytes	Query 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|\| \| ++#+ \| \| +\|\|\|\|++ \|+\|\|\| \|\|++\|\|\| +\| Sbjct 1 MAAQAAGVS#RQRAATQGLGSNQNALKYLGQDFKTLRQQC 39
Pongo pygmaeus	Query 3 GIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
synthetic construct	Query 3 GIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
Sus scrofa	Query 3 GIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39 \|++ ++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KLRAKELGLGRHENAIKYLGQDYEQLRAHC 49

* References

[PubMed ID: 9339374] Dear N, Matena K, Vingron M, Boehm T, A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution. Genomics. 1997 Oct 1;45(1):175-84.