CaMPDB :: Substrate

XSB1838 : Spectrin alpha chain, brain

This entry is computationally expanded from SB0061

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	Spectrin, non-erythroid alpha chain; Alpha-II spectrin; Fodrin alpha chain
Gene Names	Spna2, Spta2; Spna2; Spta2
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
P16086	N/A	N/A	P16086	N/A	N/A	N/A	rno:64159

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 2472 aa

Average Mass: 284.637 kDa

Monoisotopic Mass: 284.462 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Spectrin 1.	44	147	126.7	7.3e-35
Spectrin 2.	149	253	139.6	9.5e-39
Spectrin 3.	255	359	135.9	1.3e-37
Spectrin 4.	361	465	116.2	1.1e-31
Spectrin 5.	467	571	127.2	5.4e-35
Spectrin 6.	573	676	126.3	1e-34
Spectrin 7.	678	782	146.3	9.3e-41
Spectrin 8.	784	888	155.6	1.5e-43
SH3_1 1.	970	1024	78.9	1.8e-20
Spectrin 9.	1091	1231	71.0	4.4e-18
--- cleavage 1176 (inside Spectrin 1091..1231) ---
Spectrin 10.	1233	1337	147.8	3.4e-41
Spectrin 11.	1339	1442	115.6	1.6e-31
Spectrin 12.	1445	1549	119.6	1e-32
Spectrin 13.	1551	1656	100.8	4.6e-27
Spectrin 14.	1658	1762	132.2	1.6e-36
Spectrin 15.	1764	1868	149.9	7.8e-42
Spectrin 16.	1870	1974	130.9	4e-36
Spectrin 17.	1976	2081	117.1	6e-32
Spectrin 18.	2091	2195	72.2	1.9e-18
Spectrin 19.	2205	2310	68.7	2.1e-17
efhand 1.	2327	2355	36.5	1e-07
efhand 2.	2370	2398	23.4	0.00096
efhand_Ca_insen 1.	2402	2471	44.6	3.8e-10

3. Sequence Information

Fasta Sequence: XSB1838.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] VQAVQQQEVY¹¹⁷⁶-GMMPRDEADS

Tyr¹¹⁷⁶ Gly

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val¹¹⁶⁷	Gln¹¹⁶⁸	Ala¹¹⁶⁹	Val¹¹⁷⁰	Gln¹¹⁷¹	Gln¹¹⁷²	Gln¹¹⁷³	Glu¹¹⁷⁴	Val¹¹⁷⁵	Tyr¹¹⁷⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gly¹¹⁷⁷	Met¹¹⁷⁸	Met¹¹⁷⁹	Pro¹¹⁸⁰	Arg¹¹⁸¹	Asp¹¹⁸²	Glu¹¹⁸³	Ala¹¹⁸⁴	Asp¹¹⁸⁵	Ser¹¹⁸⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
LKDINKVAEDLESEGLMAEEVQAVQQQEVYGMMPRDEADSKTASPWKSARLMVHTVATFN

Summary

#	organism	max score	hits	top seq
1	N/A	95.10	4	S
2	Rattus norvegicus	95.10	3	SPTA2_RAT Spectrin alpha chain, brain (Spectrin, n
3	Homo sapiens	93.60	16	SPTA2_HUMAN Spectrin alpha chain, brain (Spectrin,
4	Mus musculus	92.80	19	PREDICTED: similar to Spectrin alpha chain, brain
5	Monodelphis domestica	92.40	2	PREDICTED: similar to spectrin alpha 2 isoform 2
6	Canis familiaris	92.40	1	PREDICTED: similar to Spectrin alpha chain, brain
7	Bos taurus	91.70	2	PREDICTED: similar to SPTAN1 protein isoform 2
8	Gallus gallus	89.40	3	spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
9	Xenopus tropicalis	68.20	1	hypothetical protein LOC100036647
10	Tetraodon nigroviridis	59.70	2	unnamed protein product
11	Danio rerio	58.90	1	alpha II-spectrin

Top-ranked sequences

organism	matching
N/A	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 93 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GMMPRDEADSKTASPWKSARLMVHTVATFN 152
Rattus norvegicus	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1147 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206
Homo sapiens	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1147 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GMMPRDETDSKTASPWKSARLMVHTVATFN 1206
Mus musculus	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1147 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GAMPRDEADSKTASPWKSARLMVHTVATFN 1206
Monodelphis domestica	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1147 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GMMPRDDTDSKTASPWKSARLMVHTVATFN 1206
Canis familiaris	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1243 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GMMPRDDTDSKTASPWKSARLMVHTVATFN 1302
Bos taurus	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#\| \|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1147 LKDINKVAEDLESEGLMAEEVQAVQQQEVY#GAMPRDESDSKTASPWKSARLMVHTVATFN 1206
Gallus gallus	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\| \|#\|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\| Sbjct 1147 LKDINKVANDLESEGLMAEEVQAVEHQEVY#GMMPRDETDSKTVSPWKSARMMVHTVATFN 1206
Xenopus tropicalis	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMPRDEADSKTASPWKSARLMVHTVATFN 1206 \|\|\|\|\|\|\|\|++\|\|\|\|\|\|+ \|#\|\| \|\|\|\|\|\|\|+\|\|\|\|\|\| \| \| \|\|\|\|\| Sbjct 1147 LKDINKVADELESEGLITEEVQPVQHQEVY#GMQ-RDEADSKSASPWKSVRTAVLAVATFN 1205
Tetraodon nigroviridis	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXXY#GMMP----RDEADSKTASPWKSARLMVHTV 1202 \|+\|\|\|\|\|\| +\|\|\|\|\|\|\| # ++ +\|\|\|\|\|\|\|\|\|\|\|\|+ \|\| \| \| Sbjct 1267 LRDINKVAAELESEGLMAEETPMVQAQQQQ#ELLGAAPGKDEADSKTASPWKNVRLAVQTT 1326 Query 1203 ATFN 1206 \| \|\| Sbjct 1327 ANFN 1330
Danio rerio	Query 1147 LKDINKVAEDLESEGLMXXXXXXXXXXXX--Y#GMMP-RDEADSKTASPWKSARLMVHTVA 1203 \|+\|\|\|\|\|\| +\|\|\|\|\|\|\| #\| \| +\|\|\|\|\|\| \|\|\|\|\|\| \|\| \| \| \| Sbjct 1147 LRDINKVASELESEGLMAEEAPVVQAQQVEVL#GSAPGKDEADSKGASPWKSVRLAVQTTA 1206 Query 1204 TFN 1206 \|\| Sbjct 1207 NFN 1209

* References

[PubMed ID: 2753883] Hong WJ, Doyle D, Cloning and analysis of cDNA clones for rat kidney alpha-spectrin. J Biol Chem. 1989 Aug 5;264(22):12758-64.

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