CaMPDB :: Substrate

XSB1869 : Beta-crystallin A3

This entry is computationally expanded from SB0069

* Basic Information

Organism	Bos taurus (cattle)
Protein Names	Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form; Contains: RecName: Full=Beta-crystallin A3; isoform A1; Delta4 form; Delta7 form; Delta8 form
Gene Names	CRYBA1
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
P11843	N/A	N/A	P11843	N/A	N/A	N/A	bta:282202

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 215 aa

Average Mass: 25.131 kDa

Monoisotopic Mass: 25.115 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 17 ---
--- cleavage 11 ---
Crystall 1.	32	116	178.5	1.8e-50
Crystall 2.	125	213	182.6	1.1e-51

3. Sequence Information

Fasta Sequence: XSB1869.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites]

Cleavage sites (±10aa)

[Site 1] QELESLPTTK¹⁷-MAQTNPMPGS

Lys¹⁷ Met

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gln⁸	Glu⁹	Leu¹⁰	Glu¹¹	Ser¹²	Leu¹³	Pro¹⁴	Thr¹⁵	Thr¹⁶	Lys¹⁷

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Met¹⁸	Ala¹⁹	Gln²⁰	Thr²¹	Asn²²	Pro²³	Met²⁴	Pro²⁵	Gly²⁶	Ser²⁷

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
METQTVQQELESLPTTKMAQTNPMPGSVGPWKITIYDQENFQGKRME

Summary

#	organism	max score	hits	top seq
1	N/A	100.00	8	-
2	Bos taurus	100.00	4	beta A3 crystallin
3	Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form	100.00	1	CRBA1_BOVIN Beta crystallin A3
4	Oryctolagus cuniculus	98.20	2	beta A3-crystallin
5	Canis lupus familiaris	97.80	2	crystallin, beta A1
6	Mus musculus	96.70	5	crystallin, beta A1
7	Rattus norvegicus	96.70	4	PREDICTED: similar to Beta crystallin A3
8	Cavia porcellus	96.70	3	beta A3-crystallin
9	Macaca mulatta	92.80	1	PREDICTED: similar to crystallin, beta A3
10	Homo sapiens	91.70	3	crystallin, beta A3
11	Pan troglodytes	91.70	2	PREDICTED: similar to Beta crystallin A3
12	Monodelphis domestica	86.30	2	PREDICTED: similar to beta A3-crystallin
13	Rana catesbeiana	69.30	4	CRB32_RANCA Beta crystallin A3-2 emb
14	Xenopus laevis	61.60	6	crystallin, beta A1
15	Gallus gallus	60.50	1	crystallin, beta A1
16	Tetraodon nigroviridis	58.90	1	unnamed protein product
17	Rana temporaria	58.20	2	CRBA1_RANTE Beta crystallin A1 emb
18	Danio rerio	54.70	6	hypothetical protein LOC436683
19	Xenopus tropicalis	53.10	1	Unknown (protein for MGC:122148)
20	rats, Sprague-Dawley, lens, Peptide Partial, 20 aa	45.40	1	beta-crystallin isoform A3 {internal fragment}
21	Canis familiaris	35.00	1	PREDICTED: similar to crystallin, beta B1
22	Cynops pyrrhogaster	33.10	1	newt beta B1-crystallin
23	Sus scrofa	32.70	1	beta crystallin subunit beta B1

Top-ranked sequences

organism	matching
N/A	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
Bos taurus	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
Oryctolagus cuniculus	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQQELETLPTTK#MAQTNPMPGSLGPWKITIYDQENFQGKRME 47
Canis lupus familiaris	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQQELETLPTTK#MAQTNPMPGSAGPWKITIYDQENFQGKRME 47
Mus musculus	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|+\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQRELETLPTTK#MAQTNPMPGSLGPWKITIYDQENFQGKRME 47
Rattus norvegicus	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|+\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQRELETLPTTK#MAQTNPMPGSMGPWKITIYDQENFQGKRME 47
Cavia porcellus	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|+\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQRELETLPTTK#MAQTNPMPGSLGPWKITIYDQENFQGKRME 47
Macaca mulatta	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQQELETLPTTK#MAQTNPTQGSLGPWKITIYDQENFQGKRME 47
Homo sapiens	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\| \|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\| \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQAEQQELETLPTTK#MAQTNPTPGSLGPWKITIYDQENFQGKRME 47
Pan troglodytes	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\| \|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\| \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQAEQQELETLPTTK#MAQTNPTPGSLGPWKITIYDQENFQGKRME 47
Monodelphis domestica	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\| \|\|+\|\|\|++\| +#\|\|\|\|\|\|\|\|\| +\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQAVQRELETVPAAR#MAQTNPMPGPLGPWKVTIYDQENFQGKRME 47
Rana catesbeiana	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\| +\| \| \| + + \|#\|\|\| \|\|+\| +\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MEIPAIQTEREDITSEK#MAQINPLPVPLGPWKITVYDQENFQGKRME 47
Xenopus laevis	Query 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\| \|\| \| \|+ \| \|#\|\|+\|\|\|+ +\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MEISPVQTERENF-TKK#MAETNPLSMRMGSWKITVYDQENFQGKRME 46
Gallus gallus	Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\|\|\|\|+\| +\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAQTNPLPVPMGPWKITVYDQENFQGKRME 30
Tetraodon nigroviridis	Query 16 TK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|#\|\|\|\|\|\|\|\| +\|\|\|\|\|\|+\|\|\|\|+\|\|\|+\|\|\| Sbjct 1 TK#MAQTNPMP--MGPWKITVYDQEHFQGRRME 30
Rana temporaria	Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|\| \|\|+\| +\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAQINPLPVPLGPWKITVYDQENFQGKRME 30
Danio rerio	Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\| \|\|\|\|\| +\|\|\|\|+\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MALTNPMP--LGPWKLTVYDQENFQGKRME 28
Xenopus tropicalis	Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 \|\|+\|\|\|+ +\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAETNPLSMRMGSWKITVYDQENFQGKRME 30
rats, Sprague-Dawley, lens, Peptide Partial, 20 aa	Query 12 SLPTTK#MAQTNPMPGSVGPW 31 +\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 1 TLPTTK#MAQTNPMPGSMGPW 20
Canis familiaris	Query 12 SLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47 ++\|\|\|\|# \| \| +\|+ +++\|\|\|\|\|\|+\|+\| Sbjct 41 TVPTTK#AGDLPP-----GSYKLVVFEQENFQGRRVE 71
Cynops pyrrhogaster	Query 27 SVGPWKITIYDQENFQGKRME 47 \|\|\| +\|\| \|++\|\|\|\|\|\|+ +\| Sbjct 44 SVGTYKIIIFEQENFQGRHLE 64
Sus scrofa	Query 19 AQTNPMPGSVGP-----WKITIYDQENFQGKRME 47 \| \|\|\| + \| +\|+ +++\|\|\|\|\|\|+\|+\| Sbjct 39 APAQPMPAAKGDLPPGSYKLVVFEQENFQGRRVE 72

[Site 2] ETQTVQQELE¹¹-SLPTTKMAQT

Glu¹¹ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu²	Thr³	Gln⁴	Thr⁵	Val⁶	Gln⁷	Gln⁸	Glu⁹	Leu¹⁰	Glu¹¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser¹²	Leu¹³	Pro¹⁴	Thr¹⁵	Thr¹⁶	Lys¹⁷	Met¹⁸	Ala¹⁹	Gln²⁰	Thr²¹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
METQTVQQELESLPTTKMAQTNPMPGSVGPWKITIYDQENF

Summary

#	organism	max score	hits	top seq
1	N/A	88.60	5	-
2	Bos taurus	88.60	2	beta A3 crystallin
3	Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form	88.60	1	CRBA1_BOVIN Beta crystallin A3
4	Oryctolagus cuniculus	86.30	2	beta A3-crystallin
5	Canis lupus familiaris	85.90	1	crystallin, beta A1
6	Cavia porcellus	84.70	2	beta A3-crystallin
7	Mus musculus	84.70	2	crystallin, beta A1
8	Rattus norvegicus	84.70	2	PREDICTED: similar to Beta crystallin A3
9	Macaca mulatta	80.90	1	PREDICTED: similar to crystallin, beta A3
10	Homo sapiens	79.70	1	crystallin, beta A3
11	Pan troglodytes	79.70	1	PREDICTED: similar to Beta crystallin A3
12	Monodelphis domestica	74.30	1	PREDICTED: similar to beta A3-crystallin
13	Rana catesbeiana	57.40	4	CRB32_RANCA Beta crystallin A3-2 emb
14	Xenopus laevis	49.70	2	crystallin, beta A1
15	Gallus gallus	48.50	1	crystallin, beta A1
16	Tetraodon nigroviridis	48.10	1	unnamed protein product
17	Rana temporaria	46.20	2	unnamed protein product
18	rats, Sprague-Dawley, lens, Peptide Partial, 20 aa	45.40	1	beta-crystallin isoform A3 {internal fragment}
19	Danio rerio	42.70	3	hypothetical protein LOC436683

Top-ranked sequences

organism	matching
N/A	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
Bos taurus	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
Oryctolagus cuniculus	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQQELE#TLPTTKMAQTNPMPGSLGPWKITIYDQENF 41
Canis lupus familiaris	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQQELE#TLPTTKMAQTNPMPGSAGPWKITIYDQENF 41
Cavia porcellus	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|+\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQRELE#TLPTTKMAQTNPMPGSLGPWKITIYDQENF 41
Mus musculus	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|+\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQRELE#TLPTTKMAQTNPMPGSLGPWKITIYDQENF 41
Rattus norvegicus	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|+\|\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQRELE#TLPTTKMAQTNPMPGSMGPWKITIYDQENF 41
Macaca mulatta	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQTVQQELE#TLPTTKMAQTNPTQGSLGPWKITIYDQENF 41
Homo sapiens	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\| \|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQAEQQELE#TLPTTKMAQTNPTPGSLGPWKITIYDQENF 41
Pan troglodytes	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\| \|\|\|\|\|#+\|\|\|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 METQAEQQELE#TLPTTKMAQTNPTPGSLGPWKITIYDQENF 41
Monodelphis domestica	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\| \|\|+\|\|\|#++\| +\|\|\|\|\|\|\|\|\| +\|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 1 METQAVQRELE#TVPAARMAQTNPMPGPLGPWKVTIYDQENF 41
Rana catesbeiana	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\| +\| \| \|# + + \|\|\|\| \|\|+\| +\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 1 MEIPAIQTERE#DITSEKMAQINPLPVPLGPWKITVYDQENF 41
Xenopus laevis	Query 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41 \|\| \|\| \| \|#+ \| \|\|\|+\|\|\|+ +\| \|\|\|\|+\|\|\|\|\|\| Sbjct 1 MEISPVQTERE#NF-TKKMAETNPLSMRMGSWKITVYDQENF 40
Gallus gallus	Query 18 MAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|+\| +\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 1 MAQTNPLPVPMGPWKITVYDQENF 24
Tetraodon nigroviridis	Query 16 TKMAQTNPMPGSVGPWKITIYDQENF 41 \|\|\|\|\|\|\|\|\|\| +\|\|\|\|\|\|+\|\|\|\|+\| Sbjct 1 TKMAQTNPMP--MGPWKITVYDQEHF 24
Rana temporaria	Query 18 MAQTNPMPGSVGPWKITIYDQENF 41 \|\|\| \|\|+\| +\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 1 MAQINPLPVPLGPWKITVYDQENF 24
rats, Sprague-Dawley, lens, Peptide Partial, 20 aa	Query 12 SLPTTKMAQTNPMPGSVGPW 31 +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 1 TLPTTKMAQTNPMPGSMGPW 20
Danio rerio	Query 18 MAQTNPMPGSVGPWKITIYDQENF 41 \|\| \|\|\|\|\| +\|\|\|\|+\|+\|\|\|\|\|\| Sbjct 1 MALTNPMP--LGPWKLTVYDQENF 22

* References

[PubMed ID: 6547897] Gorin MB, Horwitz J, Cloning and characterization of a cow beta crystallin cDNA. Curr Eye Res. 1984 Jul;3(7):939-48.

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