CaMPDB :: Substrate

XSB1882 : neurotensin/neuromedin N preproprotein [Homo sapiens]

This entry is computationally expanded from SB0034

* Basic Information

Organism	Homo sapiens (human)
Protein Names	Neurotensin/neuromedin N; Large neuromedin N; NmN-125; Neuromedin N; NmN; NN; Neurotensin; NT; Tail peptide; neurotensin/neuromedin N preproprotein
Gene Names	NTS; neurotensin
Gene Locus	12q21; chromosome 12
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_006174	NM_006183	4922	P30990	162650	8038	N/A	hsa:4922

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 170 aa

Average Mass: 19.795 kDa

Monoisotopic Mass: 19.782 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Pro-NT_NN 1.	2	170	467.2	2.3e-137
--- cleavage 153 (inside Pro-NT_NN 2..170) ---
--- cleavage 158 (inside Pro-NT_NN 2..170) ---

3. Sequence Information

Fasta Sequence: XSB1882.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 3F6K (X-ray; 200 A; N=152-163)

* Cleavage Information

2 [sites]

Cleavage sites (±10aa)

[Site 1] IPYILKRQLY¹⁵³-ENKPRRPYIL

Tyr¹⁵³ Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile¹⁴⁴	Pro¹⁴⁵	Tyr¹⁴⁶	Ile¹⁴⁷	Leu¹⁴⁸	Lys¹⁴⁹	Arg¹⁵⁰	Gln¹⁵¹	Leu¹⁵²	Tyr¹⁵³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu¹⁵⁴	Asn¹⁵⁵	Lys¹⁵⁶	Pro¹⁵⁷	Arg¹⁵⁸	Arg¹⁵⁹	Pro¹⁶⁰	Tyr¹⁶¹	Ile¹⁶²	Leu¹⁶³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
EDILDTGNDKNGKEEVIKRKIPYILKRQLYENKPRRPYILKRDSYYY

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	101.00	3	neurotensin/neuromedin N preproprotein
2	Pan troglodytes	98.20	3	PREDICTED: neurotensin/neuromedin N isoform 3
3	Macaca fascicularis	98.20	1	hypothetical protein
4	Macaca mulatta	98.20	1	PREDICTED: neurotensin
5	Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	93.60	2	NEUT_BOVIN Neurotensin/neuromedin N precursor
6	Bos taurus	93.60	1	neurotensin
7	Canis familiaris	93.20	1	PREDICTED: similar to Neurotensin/neuromedin N pre
8	Mus musculus	90.10	1	neurotensin
9	Rattus norvegicus	88.60	1	PREDICTED: similar to Neurotensin/neuromedin N pre
10	N/A	88.60	1	-
11	Monodelphis domestica	70.10	1	PREDICTED: similar to Neurotensin/neuromedin N pre
12	Xenopus laevis	59.30	1	MGC84018 protein
13	Gallus gallus	58.20	1	PREDICTED: similar to Neurotensin/neuromedin N pre

Top-ranked sequences

organism	matching
Homo sapiens	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170
Pan troglodytes	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 124 EDILDTGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170
Macaca fascicularis	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 124 EDILDTGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170
Macaca mulatta	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 124 EDILDTGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170
Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 123 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 169
Bos taurus	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170
Canis familiaris	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|+\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 124 EDVLDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170
Mus musculus	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 123 EDILDNVNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 169
Rattus norvegicus	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\| \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 123 EDILDHGNEKTEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRASYYY 169
N/A	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 \|\|\|\|\| \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 79 EDILDHGNEKTEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRASYYY 125
Monodelphis domestica	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170 +\|\|\|+\|\|\| \|\|\|\|+\|\|\| \|\|\|\|\|\|\|++# \|\| \|\|\|\|\|\|\|\| \| \|\| Sbjct 119 DDILETGNLNREKEEVMKRKTPYILKRQIH#VNKARRPYILKRSSSYY 165
Xenopus laevis	Query 125 DILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYY 169 \| \|+ + + \|+\| \|\|\|\| \|\|\|\|\|\|\| \|# +\| \|\|\|\|\|\|\|\| \|\|\| Sbjct 122 DYLEPNSPNSEKDEPIKRKTPYILKRQTY#VSKARRPYILKRGSYY 166
Gallus gallus	Query 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYY 169 +\| \| \| \|\| ++\|\|\| \|\|\|\|\|\|\|\|+# \|\| \|\|\|\|\|\|\|\| \|\|\| Sbjct 120 QDAFDLENSSQEKE-IMKRKNPYILKRQLH#VNKARRPYILKRSSYY 164

[Site 2] KRQLYENKPR¹⁵⁸-RPYILKRDSY

Arg¹⁵⁸ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys¹⁴⁹	Arg¹⁵⁰	Gln¹⁵¹	Leu¹⁵²	Tyr¹⁵³	Glu¹⁵⁴	Asn¹⁵⁵	Lys¹⁵⁶	Pro¹⁵⁷	Arg¹⁵⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹⁵⁹	Pro¹⁶⁰	Tyr¹⁶¹	Ile¹⁶²	Leu¹⁶³	Lys¹⁶⁴	Arg¹⁶⁵	Asp¹⁶⁶	Ser¹⁶⁷	Tyr¹⁶⁸

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
TGNDKNGKEEVIKRKIPYILKRQLYENKPRRPYILKRDSYYY

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	91.70	3	neurotensin/neuromedin N preproprotein
2	Pan troglodytes	88.60	3	PREDICTED: neurotensin/neuromedin N isoform 3
3	Macaca fascicularis	88.60	1	hypothetical protein
4	Macaca mulatta	88.60	1	PREDICTED: neurotensin
5	Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	84.00	2	NEUT_BOVIN Neurotensin/neuromedin N precursor
6	Bos taurus	84.00	1	neurotensin
7	Canis familiaris	84.00	1	PREDICTED: similar to Neurotensin/neuromedin N pre
8	Mus musculus	81.60	1	neurotensin
9	Rattus norvegicus	79.70	1	PREDICTED: similar to Neurotensin/neuromedin N pre
10	N/A	79.70	1	-
11	Monodelphis domestica	63.20	1	PREDICTED: similar to Neurotensin/neuromedin N pre
12	Gallus gallus	56.20	1	PREDICTED: similar to Neurotensin/neuromedin N pre
13	Xenopus laevis	55.80	1	MGC84018 protein

Top-ranked sequences

organism	matching
Homo sapiens	Query 129 TGNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Query 129 TGNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170
Pan troglodytes	Query 129 TGNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 129 TGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170
Macaca fascicularis	Query 129 TGNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 129 TGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170
Macaca mulatta	Query 129 TGNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 129 TGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170
Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	Query 130 GNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 129 GNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 169
Bos taurus	Query 130 GNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 130 GNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170
Canis familiaris	Query 130 GNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 130 GNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170
Mus musculus	Query 131 NDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 130 NDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 169
Rattus norvegicus	Query 130 GNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 129 GNEKTEKEEVIKRKIPYILKRQLYENKPR#RPYILKRASYYY 169
N/A	Query 130 GNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 85 GNEKTEKEEVIKRKIPYILKRQLYENKPR#RPYILKRASYYY 125
Monodelphis domestica	Query 129 TGNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170 \|\|\| \|\|\|\|+\|\|\| \|\|\|\|\|\|\|++ \|\| \|#\|\|\|\|\|\|\| \| \|\| Sbjct 124 TGNLNREKEEVMKRKTPYILKRQIHVNKAR#RPYILKRSSSYY 165
Gallus gallus	Query 136 KEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYY 169 ++\|++\|\|\| \|\|\|\|\|\|\|\|+ \|\| \|#\|\|\|\|\|\|\| \|\|\| Sbjct 131 EKEIMKRKNPYILKRQLHVNKAR#RPYILKRSSYY 164
Xenopus laevis	Query 136 KEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYY 169 \|+\| \|\|\|\| \|\|\|\|\|\|\| \| +\| \|#\|\|\|\|\|\|\| \|\|\| Sbjct 133 KDEPIKRKTPYILKRQTYVSKAR#RPYILKRGSYY 166

* References

[PubMed ID: 19086053] Gratacos M, Costas J, de Cid R, Bayes M, Gonzalez JR, Baca-Garcia E, de Diego Y, Fernandez-Aranda F, Fernandez-Piqueras J, Guitart M, Martin-Santos R, Martorell L, Menchon JM, Roca M, Saiz-Ruiz J, Sanjuan J, Torrens M, Urretavizcaya M, Valero J, Vilella E, Estivill X, Carracedo A, Identification of new putative susceptibility genes for several psychiatric disorders by association analysis of regulatory and non-synonymous SNPs of 306 genes involved in neurotransmission and neurodevelopment. Am J Med Genet B Neuropsychiatr Genet. 2008 Dec 11.

» More (9) « Hide Below

[PubMed ID: 18624930] ... Giorgi RR, Chile T, Bello AR, Reyes R, Fortes MA, Machado MC, Cescato VA, Musolino NR, Bronstein MD, Giannella-Neto D, Correa-Giannella ML, Expression of neurotensin and its receptors in pituitary adenomas. J Neuroendocrinol. 2008 Sep;20(9):1052-7. Epub 2008 Jul 8.

[PubMed ID: 17644423] ... Xiong L, Levchenko A, Montplaisir J, Riviere JB, Thibodeau P, St-Onge J, Gaspar C, Desautels A, Lesperance P, Chouinard S, Turecki G, Rouleau GA, Genetic association studies of neurotensin gene and restless legs syndrome in French Canadians. Sleep Med. 2008 Mar;9(3):273-82. Epub 2007 Jul 17.

[PubMed ID: 18048355] ... Li J, Chen LA, Townsend CM Jr, Evers BM, PKD1, PKD2, and their substrate Kidins220 regulate neurotensin secretion in the BON human endocrine cell line. J Biol Chem. 2008 Feb 1;283(5):2614-21. Epub 2007 Nov 29.

[PubMed ID: 17870207] ... Bossard C, Souaze F, Jarry A, Bezieau S, Mosnier JF, Forgez P, Laboisse CL, Over-expression of neurotensin high-affinity receptor 1 (NTS1) in relation with its ligand neurotensin (NT) and nuclear beta-catenin in inflammatory bowel disease-related oncogenesis. Peptides. 2007 Oct;28(10):2030-5. Epub 2007 Jul 25.

[PubMed ID: 1436492] ... Bean AJ, Dagerlind A, Hokfelt T, Dobner PR, Cloning of human neurotensin/neuromedin N genomic sequences and expression in the ventral mesencephalon of schizophrenics and age/sex matched controls. Neuroscience. 1992 Sep;50(2):259-68.

[PubMed ID: 3670596] ... Mai JK, Triepel J, Metz J, Neurotensin in the human brain. Neuroscience. 1987 Aug;22(2):499-524.

[PubMed ID: 3525564] ... Checler F, Vincent JP, Kitabgi P, Purification and characterization of a novel neurotensin-degrading peptidase from rat brain synaptic membranes. J Biol Chem. 1986 Aug 25;261(24):11274-81.

[PubMed ID: 6754769] ... Jennes L, Stumpf WE, Kalivas PW, Neurotensin: topographical distribution in rat brain by immunohistochemistry. J Comp Neurol. 1982 Sep 20;210(3):211-24.

[PubMed ID: 409699] ... Helmstaedter V, Taugner C, Feurle GE, Forssmann WG, Localization of neurotensin-immunoreactive cells in the small intestine of man and various mammals. Histochemistry. 1977 Jul 18;53(1):35-41.