XSB1894 : Calpain small subunit 1
[ CaMP Format ]
This entry is computationally expanded from SB0017
* Basic Information
| Organism | Mus musculus (house mouse) |
| Protein Names | CSS1; Calcium-dependent protease small subunit 1; Calcium-dependent protease small subunit; CDPS; Calpain regulatory subunit; Calcium-activated neutral proteinase small subunit; CANP small subunit |
| Gene Names | Capn4 |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 269 aa
Average Mass: 28.463 kDa
Monoisotopic Mass: 28.445 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| TAT_signal 1. | 6 | 26 | 2.4 | 8.7 |
| --- cleavage 142 --- | ||||
| efhand 1. | 144 | 172 | 18.4 | 0.03 |
| efhand 2. | 174 | 202 | 16.7 | 0.098 |
| efhand 3. | 210 | 237 | 0.4 | 19 |
| efhand 4. | 239 | 266 | 7.5 | 3.2 |
3. Sequence Information
Fasta Sequence: XSB1894.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
1 [sites]
Cleavage sites (±10aa)
[Site 1] PDLKTDGFGI142-DTCRSMVAVM
Ile142 
Asp
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Pro133 | Asp134 | Leu135 | Lys136 | Thr137 | Asp138 | Gly139 | Phe140 | Gly141 | Ile142 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Asp143 | Thr144 | Cys145 | Arg146 | Ser147 | Met148 | Val149 | Ala150 | Val151 | Met152 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| DMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNI |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 126.00 | 38 | Calpain, small subunit 1 |
| 2 | Mus musculus | 126.00 | 33 | calpain, small subunit 1 |
| 3 | N/A | 126.00 | 27 | C |
| 4 | Rattus norvegicus | 126.00 | 13 | calpain, small subunit 1 |
| 5 | Bos taurus | 126.00 | 8 | calpain, small subunit 1 |
| 6 | Sus scrofa | 126.00 | 7 | calpain, small subunit 1 |
| 7 | Ovis aries | 125.00 | 3 | calpain II small subunit |
| 8 | Oryctolagus cuniculus | 125.00 | 2 | calpain, small subunit 1 |
| 9 | Gallus gallus | 123.00 | 8 | calpain small subunit |
| 10 | Canis familiaris | 110.00 | 23 | PREDICTED: similar to calpain small subunit 2 |
| 11 | Pan troglodytes | 110.00 | 8 | PREDICTED: acyltransferase like 1 |
| 12 | Macaca mulatta | 110.00 | 4 | PREDICTED: similar to calpain small subunit 2 |
| 13 | Xenopus laevis | 109.00 | 13 | LOC446963 protein |
| 14 | Xenopus tropicalis | 108.00 | 6 | calpain, small subunit 2 |
| 15 | Danio rerio | 105.00 | 26 | hypothetical protein LOC550598 |
| 16 | Monodelphis domestica | 103.00 | 4 | PREDICTED: similar to Calpain, small subunit 2 |
| 17 | Oncorhynchus mykiss | 98.20 | 5 | calpain regulatory subunit |
| 18 | Tetraodon nigroviridis | 94.00 | 10 | unnamed protein product |
| 19 | Coturnix coturnix | 80.10 | 1 | quail calpain |
| 20 | Macaca fascicularis | 79.30 | 1 | CAN3_MACFA Calpain-3 (Calpain L3) (Calpain p94) (C |
| 21 | synthetic construct | 77.40 | 1 | Homo sapiens calpain 1, (mu/I) large subunit |
| 22 | Pongo pygmaeus | 76.30 | 1 | CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1 |
| 23 | Stizostedion vitreum vitreum | 74.30 | 1 | putative calpain-like protein |
| 24 | catalytic | 70.10 | 1 | Calpain 2, large |
| 25 | Strongylocentrotus purpuratus | 65.10 | 1 | PREDICTED: similar to calpain B isoform 2 |
| 26 | Schistosoma japonicum | 59.70 | 2 | unknown |
| 27 | Tribolium castaneum | 57.80 | 1 | PREDICTED: similar to CG8107-PA |
| 28 | Gecarcinus lateralis | 57.00 | 1 | calpain B |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |
| Mus musculus | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |
| N/A | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |
| Rattus norvegicus | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |
| Bos taurus | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |
| Sus scrofa | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |
| Ovis aries | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||||||||||||||||||||||||||||+#|||||||||||||||||||||||||||||| Sbjct 28 DMEVSATELMNILNKVVTRHPDLKTDGFGV#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 87 |
| Oryctolagus cuniculus | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||||||||||||||||||||||||||||+#|||||||||||||||||||||||||||||| Sbjct 110 DMEVSATELMNILNKVVTRHPDLKTDGFGL#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 169 |
| Gallus gallus | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||| |||||||||||||||||||||||+#|||||||||||||||||||||||||||||| Sbjct 58 DMEVSPTELMNILNKVVTRHPDLKTDGFGL#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 117 |
| Canis familiaris | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||| ||+||||||||+++| ||||||| +#|||||+|+|||||||||||||||||||||| Sbjct 95 DMEVGATDLMNILNKVLSKHKDLKTDGFSL#DTCRSIVSVMDSDTTGKLGFEEFKYLWNNI 154 |
| Pan troglodytes | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||| ||+||||||||+++| ||||||| +#|||||+|+|||||||||||||||||||||| Sbjct 492 DMEVGATDLMNILNKVLSKHKDLKTDGFSL#DTCRSIVSVMDSDTTGKLGFEEFKYLWNNI 551 |
| Macaca mulatta | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||| ||+||||||||+++| ||||||| +#|||||+|+|||||||||||||||||||||| Sbjct 92 DMEVGATDLMNILNKVLSKHKDLKTDGFSL#DTCRSIVSVMDSDTTGKLGFEEFKYLWNNI 151 |
| Xenopus laevis | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||||| ||| |||||| || ||||||| +#|+|||||||||||+|||||||||||||+|| Sbjct 71 DMEVSAAELMGILNKVVARHQDLKTDGFSV#DSCRSMVAVMDSDSTGKLGFEEFKYLWDNI 130 |
| Xenopus tropicalis | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||||||||| |||||+ +| ||||||| #|+|||||||||||+|||||||||||||+|| Sbjct 78 DMEVSATELMGILNKVIAKHQDLKTDGFSA#DSCRSMVAVMDSDSTGKLGFEEFKYLWDNI 137 |
| Danio rerio | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||| |||||||||++++| ||||||| |#++|||||||||||+|||||| |||+||||| Sbjct 60 DMEVSPTELMNILNKIISKHGDLKTDGFTI#ESCRSMVAVMDSDSTGKLGFHEFKHLWNNI 119 |
| Monodelphis domestica | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||| +||||||||+++| +| |||| +#|||||+||||||| |||||||||||||||| Sbjct 89 DMEVGPADLMNILNKVISKHRELNTDGFSL#DTCRSIVAVMDSDATGKLGFEEFKYLWNNI 148 |
| Oncorhynchus mykiss | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||| ||||||||+++ + ||||||| |#++|| ||||||||+|||||| | |+||||| Sbjct 60 DMEVSPTELMNILNRIIGKRSDLKTDGFSI#ESCRGMVAVMDSDSTGKLGFHELKFLWNNI 119 |
| Tetraodon nigroviridis | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||||| || +|||||+ | ++||||| |#++|||||||||||+|||||| |||||| || Sbjct 61 DMEVSPGELKDILNKVIGRFSNMKTDGFSI#ESCRSMVAVMDSDSTGKLGFHEFKYLWGNI 120 |
| Coturnix coturnix | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||+| || |||+|+ || ||||||| +#|+||+|| +|| | + +|| ||+ ||| | Sbjct 549 DMEISVFELKTILNRVIARHKDLKTDGFSL#DSCRNMVNLMDKDGSARLGLVEFQILWNKI 608 |
| Macaca fascicularis | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||+ | || +|| || +| |||| || +#++||||+|+||+| +||| +|| +||| | Sbjct 659 DMEICADELKKVLNTVVNKHKDLKTHGFTL#ESCRSMIALMDTDGSGKLNLQEFHHLWNKI 718 |
| synthetic construct | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |||+| || |||+++++| ||+| || +#++||||| +|| | |||| || ||| | Sbjct 558 DMEISVKELRTILNRIISKHKDLRTKGFSL#ESCRSMVNLMDRDGNGKLGLVEFNILWNRI 617 |
| Pongo pygmaeus | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 ||| | || |||+++++| ||+| || +#++||||| +|| | |||| || ||| | Sbjct 558 DMETSVKELRTILNRIISKHKDLRTKGFSL#ESCRSMVNLMDRDGNGKLGLVEFNILWNRI 617 |
| Stizostedion vitreum vitreum | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 | |+|+ || |||+|||+ |+|| || |# |||+|| ++| | +|||| ||| || | Sbjct 546 DCEISSYELQKILNRVVTKRSDIKTSGFSI#TTCRNMVNMLDKDGSGKLGLLEFKVLWTKI 605 |
| catalytic | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 | |+|| || || +|+ + |+|+||| |#+||+ || ++||| +|||| +|| || | Sbjct 574 DAEISAFELQTILRRVLAKRQDIKSDGFSI#ETCKIMVDMLDSDGSGKLGLKEFYILWTKI 633 |
| Strongylocentrotus purpuratus | Query 113 DMEVSATELMNILNKVVTRH----PDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYL 168 |||| | || ||| + + +|+ || +#++|+||||+ | | +||||||||+ | Sbjct 529 DMEVDAWELQEILNAALRKDLQVPGELQGGGFSL#ESCKSMVALTDDDRSGKLGFEEFREL 588 Query 169 WNNI 172 | || Sbjct 589 WQNI 592 |
| Schistosoma japonicum | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWN 170 | |+ | || +||| || | ||| +#++||||+|+|| | +| | | ||+ ||+ Sbjct 201 DGEIDADELRDILNCAFTR--DFTFDGFSL#ESCRSMIAMMDFDRSGMLSFPEFRKLWD 256 |
| Tribolium castaneum | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 | || || ||+ || + || #| ||||+|++| | |||| ||||| || +| Sbjct 960 DEEVDWKELKEILDHY-TRKVKTQNRGFSK#DVCRSMIAMLDVDRTGKLNFEEFKRLWESI 1018 |
| Gecarcinus lateralis | Query 113 DMEVSATELMNILNKVVTRHPDLKTDGFGI#DTCRSMVAVMDSDTTGKLGFEEFKYLWNNI 172 |+|+ || ++|| + | + +|| #| ||||+|+|| | +|||| +|| || +| Sbjct 600 DLEIDWKELQDVLNFALKR--EFNFEGFSK#DVCRSMIAMMDVDRSGKLGLQEFLQLWMDI 657 |
* References
[PubMed ID: 9774740] Arthur JS, Greer PA, Elce JS, Structure of the mouse calpain small subunit gene. Biochim Biophys Acta. 1998 Oct 14;1388(1):247-52.
[PubMed ID: 10825211] ... Arthur JS, Elce JS, Hegadorn C, Williams K, Greer PA, Disruption of the murine calpain small subunit gene, Capn4: calpain is essential for embryonic development but not for cell growth and division. Mol Cell Biol. 2000 Jun;20(12):4474-81.
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