CaMPDB :: Substrate

XSB1932 : dystrophin [Sus scrofa]

This entry is computationally expanded from SB0073

* Basic Information

Organism	Sus scrofa (pig)
Protein Names	dystrophin
Gene Names	DMD; dystrophin
Gene Locus	X; chromosome X
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_001012408	NM_001012408	497636	Q5GN48	N/A	N/A	N/A	ssc:497636

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 3674 aa

Average Mass: 424.858 kDa

Monoisotopic Mass: 424.594 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
CH 1.	12	115	111.4	3e-30
CH 2.	131	236	102.8	1.2e-27
Spectrin 1.	335	443	69.2	1.5e-17
Spectrin 2.	444	552	63.1	1.1e-15
--- cleavage 686 ---
Spectrin 3.	715	823	12.4	0.0031
Spectrin 4.	826	929	25.5	0.00022
Spectrin 5.	939	1041	16.8	0.0013
Spectrin 6.	1044	1150	79.6	1.1e-20
Spectrin 7.	1153	1259	54.3	4.7e-13
Spectrin 8.	1262	1363	17.8	0.0011
DUF1136 1.	1409	1433	-4.6	5.5
KID 1.	1485	1495	2.4	1.7e
Spectrin 9.	1567	1672	47.5	5.2e-11
Spectrin 10.	1675	1775	63.1	1.1e-15
Involucrin 1.	1845	1854	2.3	1.3e
Spectrin 11.	1873	1975	45.5	2e-10
Spectrin 12.	1991	2097	9.0	0.0059
Spectrin 13.	2100	2204	76.8	8.1e-20
Spectrin 14.	2207	2314	53.6	7.5e-13
Involucrin 2.	2319	2328	2.3	1.3e
Spectrin 15.	2459	2566	19.0	0.00084
Spectrin 16.	2569	2675	42.7	1.4e-09
Spectrin 17.	2678	2791	24.6	0.00029
Spectrin 18.	2923	3029	69.4	1.3e-17
WW 1.	3046	3075	48.9	2e-11
efhand_1 1.	3076	3196	274.4	2.6e-79
efhand_2 1.	3200	3291	230.0	6.1e-66
ZZ 1.	3296	3341	93.3	8.6e-25
M 1.	3490	3510	9.4	8.3
M 2.	3556	3576	8.1	12

3. Sequence Information

Fasta Sequence: XSB1932.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] VMETVTMVTT⁶⁸⁶-REQILVKHAQ

Thr⁶⁸⁶ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val⁶⁷⁷	Met⁶⁷⁸	Glu⁶⁷⁹	Thr⁶⁸⁰	Val⁶⁸¹	Thr⁶⁸²	Met⁶⁸³	Val⁶⁸⁴	Thr⁶⁸⁵	Thr⁶⁸⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg⁶⁸⁷	Glu⁶⁸⁸	Gln⁶⁸⁹	Ile⁶⁹⁰	Leu⁶⁹¹	Val⁶⁹²	Lys⁶⁹³	His⁶⁹⁴	Ala⁶⁹⁵	Gln⁶⁹⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SSTQISQAVTTTQPSLTQTTVMETVTMVTTREQILVKHAQEELPPPPPQKKRQIIVDSEI

Summary

#	organism	max score	hits	top seq
1	Sus scrofa	48.50	1	dystrophin
2	Bos taurus	48.50	1	PREDICTED: similar to dystrophin, partial
3	Canis familiaris	46.60	1	PREDICTED: hypothetical protein XP_850502
4	Canis lupus familiaris	46.60	1	dystrophin
5	Homo sapiens	43.50	15	dystrophin (muscular dystrophy, Duchenne and Becke
6	N/A	43.50	3	D
7	Macaca mulatta	43.50	1	PREDICTED: dystrophin, partial
8	Mus musculus	43.10	1	dystrophin, muscular dystrophy
9	Monodelphis domestica	42.00	1	PREDICTED: similar to T cell antigen receptor alph
10	Gallus gallus	39.30	1	dystrophin
11	Danio rerio	32.70	1	PREDICTED: dystrophin

Top-ranked sequences

organism	matching
Sus scrofa	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|\|\|\|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 657 SSTQISQAVTTTQPSLTQTTVMETVTMVTT#REQILVKHAQEELPPPPPQKKRQIIVDSEI 716
Bos taurus	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|\|\|\|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 57 SSTQISQAVTTTQPSLTQTTVMETVTMVTT#REQILVKHAQEELPPPPPQKKRQIIVDSEI 116
Canis familiaris	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|\| \|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 539 SSAQISQAVTTTQPSLTQTTVMETVTMVTT#REHILVKHAQEELPPPPPQKKRQIIVDSEI 598
Canis lupus familiaris	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|\| \|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 662 SSAQISQAVTTTQPSLTQTTVMETVTMVTT#REHILVKHAQEELPPPPPQKKRQIIVDSEI 721
Homo sapiens	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|+ \|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \|\|\|\|\| Sbjct 661 STAQISQAVTTTQPSLTQTTVMETVTTVTT#REQILVKHAQEELPPPPPQKKRQITVDSEI 720
N/A	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|+ \|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \|\|\|\|\| Sbjct 661 STAQISQAVTTTQPSLTQTTVMETVTTVTT#REQILVKHAQEELPPPPPQKKRQITVDSEI 720
Macaca mulatta	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|+ \|\|\|\|\|\| # \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \|\|\|\|\| Sbjct 363 STAQISQAVTTTQPSLTQTTVMETVTMVTT#REQILVKHAQEELPPPPPQKKRQITVDSEI 422
Mus musculus	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|\| \|\|\|\|\|\| # \|+\|\|\|\|\|\|\|\| \|\|\|\|\|\| \|\|\|\|+ Sbjct 663 SSAQISQAVTTTQPSLTQTTVMETVTMVTT#REQIMVKHAQEELPPPPPQKKRQITVDSEL 722
Monodelphis domestica	Query 657 SSTQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \|\| \|\|\|\|\|\| # \|\|\|\|\|\|+\|\| \|\|\|\|\|\|+\|\|\|\|\| Sbjct 656 SSVQISQAVTTTQTSLTQTTVMETVTMVTT#REQILVKHAKEEPLPPPPQKKRQIVVDSEI 715
Gallus gallus	Query 658 STQISQAVXXXXXXXXXXXXXXXXXXXXX#XXXILVKHAQEELXXXXXQKKRQIIVDSEI 716 \| \|+\|\|\|\| # \|\|\|\|\|\|+\|\|\| \|\|\|\|++\|\|\|\|\| Sbjct 664 SKQVSQAVTTTQTSLTQTTVMETVTMVTT#REQILVKHAKEELPPPPPHKKRQLLVDSEI 722
Danio rerio	Query 691 LVKHAQEELXXXXXQKKRQIIVDSEI 716 +\|\|\| +\| + \|\|\|\|\|\|+\|\|\|\|+ Sbjct 715 MVKHTKEGMSTPPPQKKRQIVVDSEL 740

* References

[PubMed ID: 15941659] Bordais A, Bolanos-Jimenez F, Fort P, Varela C, Sahel JA, Picaud S, Rendon A, Molecular cloning and protein expression of Duchenne muscular dystrophy gene products in porcine retina. Neuromuscul Disord. 2005 Jul;15(7):476-87.