CaMPDB :: Substrate

XSB1986 : crystallin, beta A4 [Mus musculus]

This entry is computationally expanded from SB0080

* Basic Information

Organism	Mus musculus (house mouse)
Protein Names	Beta-crystallin A4; Beta-A4 crystallin; crystallin; beta A4
Gene Names	Cryba4; crystallin, beta A4
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_067326	NM_021351	12959	Q9JJV0	N/A	N/A	N/A	mmu:12959

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 196 aa

Average Mass: 22.469 kDa

Monoisotopic Mass: 22.455 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 4 ---
Crystall 1.	13	97	162.8	1e-45
Crystall 2.	106	194	168.4	2.2e-47

3. Sequence Information

Fasta Sequence: XSB1986.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] MTLQ⁴-CTKSAGHWRM

Gln⁴ Cys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	-	-	-	-	Met¹	Thr²	Leu³	Gln⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Cys⁵	Thr⁶	Lys⁷	Ser⁸	Ala⁹	Gly¹⁰	His¹¹	Trp¹²	Arg¹³	Met¹⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MTLQCTKSAGHWRMVVWDEEGFQGRRHEFTAECP

Summary

#	organism	max score	hits	top seq
1	Mus musculus	80.90	9	unnamed protein product
2	Rattus norvegicus	79.30	6	crystallin, beta A4
3	Macaca mulatta	78.60	2	PREDICTED: similar to Beta crystallin A4 (Beta-A4-
4	Canis lupus familiaris	78.20	2	crystallin, beta A4
5	Bos taurus	76.60	6	beta A4 crystallin
6	Homo sapiens	74.70	8	crystallin, beta A4
7	Pan troglodytes	74.70	6	PREDICTED: crystallin, beta A4
8	Monodelphis domestica	72.00	3	PREDICTED: similar to Beta crystallin A4 (Beta-A4-
9	Xenopus tropicalis	63.90	2	Unknown (protein for MGC:121449)
10	Tetraodon nigroviridis	58.50	2	unnamed protein product
11	Danio rerio	57.00	5	hypothetical protein LOC553178
12	rats, Sprague-Dawley, lens, Peptide Partial, 24 aa	56.60	1	beta-crystallin isoform A4 {N-terminal}
13	N/A	45.80	9	J
14	Gallus gallus	45.80	4	crystallin, beta A4
15	Xenopus laevis	45.40	3	hypothetical protein LOC495288
16	Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form	41.20	1	CRBA1_BOVIN Beta crystallin A3
17	Oryctolagus cuniculus	40.80	2	beta A1-crystallin
18	Cavia porcellus	38.10	2	beta A1-crystallin
19	Iguana iguana	37.70	1	betaA2-crystallin
20	Rana catesbeiana	37.40	4	CRB12_RANCA Beta crystallin A1-2 emb
21	Rana temporaria	37.40	2	CRBA1_RANTE Beta crystallin A1 emb
22	Contains: Beta crystallin B1B	36.20	1	CRBB1_RAT Beta crystallin B1
23	Ovis aries	35.40	1	crystallin, beta B3
24	synthetic construct	35.40	1	crystallin beta B1
25	Sus scrofa	35.00	1	beta crystallin subunit beta B1
26	Canis familiaris	35.00	1	PREDICTED: similar to crystallin, beta B1

Top-ranked sequences

organism	matching
Mus musculus	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34
Rattus norvegicus	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTLQ#CTKSAGHWRVVVWDEEGFQGRRHEFTAECP 34
Macaca mulatta	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\|\|\|#\|\|\|\|\|\|\|\|+\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 12 MTLQ#CTKSAGHWKMVVWDEDGFQGRRHEFTAECP 45
Canis lupus familiaris	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\|\|\|#\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTLQ#CTKSAGHWKIVVWDEEGFQGRRHEFTAECP 34
Bos taurus	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|+\|\|#\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSLQ#CTKSAGHWKIVVWDEEGFQGRRHEFTAECP 34
Homo sapiens	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\|\|\|#\|\|\|\|\|\| \|+\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTLQ#CTKSAGPWKMVVWDEDGFQGRRHEFTAECP 34
Pan troglodytes	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\|\|\|#\|\|\|\|\|\| \|+\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTLQ#CTKSAGPWKMVVWDEDGFQGRRHEFTAECP 34
Monodelphis domestica	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|+ \|#\|\|\|\|+\|\|\|++\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSQQ#CTKSSGHWKIVVWDQEGFQGRRHEFTAECP 34
Xenopus tropicalis	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAEC 33 \|\| #\|\|\| +\|\|\|+++\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTQH#CTKFSGHWKIIVWDEECFQGRRHEFTAEC 33
Tetraodon nigroviridis	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAEC 33 \|\| #\|\|\| +\|\|\|+++\|+\|\|\| \|\|\|\|\|\|\|\|\|+\|\| Sbjct 1 MTHH#CTKFSGHWKIIVFDEECFQGRRHEFTSEC 33
Danio rerio	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAEC 33 \|\| #\|\|\| +\|\|\|+++\|+\|\|\| \|\|\|\| \|\|\|\|+\|\| Sbjct 1 MTHH#CTKFSGHWKIIVYDEECFQGRHHEFTSEC 33
rats, Sprague-Dawley, lens, Peptide Partial, 24 aa	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQG 24 \|\|\|\|#\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| Sbjct 1 MTLQ#CTKSAGHWRVVVWDEEGFQG 24
N/A	Query 8 SAGHWRMVVWDEEGFQGRRHEFTAEC 33 \|+\| \|++\|\|\|\|\| \|\|\|+\|\|\|\|\| +\| Sbjct 8 SSGLWKIVVWDEPFFQGKRHEFTTDC 33
Gallus gallus	Query 8 SAGHWRMVVWDEEGFQGRRHEFTAEC 33 \|+\| \|++\|\|\|\|\| \|\|\|+\|\|\|\|\| +\| Sbjct 8 SSGLWKIVVWDEPFFQGKRHEFTTDC 33
Xenopus laevis	Query 1 MTLQ#CTKSAGHWRMVVWDEEGFQGRRHEFTAECP 34 \|\| # ++ \| \|+\| \|\|+\|\| \|\|\|+\| \|\| \|\|\| Sbjct 1 MTSH#PMETQGQWKMTVWEEENFQGKRCEFHLECP 34
Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form	Query 8 SAGHWRMVVWDEEGFQGRRHEFTAECP 34 \| \| \|++ ++\|+\| \|\|\|+\| \|\|\|+ \|\| Sbjct 27 SVGPWKITIYDQENFQGKRMEFTSSCP 53
Oryctolagus cuniculus	Query 8 SAGHWRMVVWDEEGFQGRRHEFTAECP 34 \| \| \|++ ++\|+\| \|\|\|+\| \|\|\|+ \|\| Sbjct 10 SLGPWKITIYDQENFQGKRMEFTSSCP 36
Cavia porcellus	Query 8 SAGHWRMVVWDEEGFQGRRHEFTAEC 33 \| \| \|++ ++\|+\| \|\|\|+\| \|\|\|+ \| Sbjct 10 SLGPWKITIYDQENFQGKRMEFTSSC 35
Iguana iguana	Query 10 GHWRMVVWDEEGFQGRRHEFTAECP 34 \| +++ \|\|+\|\| \|\|\| \| \|\| \|\|\| Sbjct 3 GQFKITVWEEENFQGERCEFLMECP 27
Rana catesbeiana	Query 10 GHWRMVVWDEEGFQGRRHEFTAEC 33 \| \|++ \|+\|+\| \|\|\|+\| \|\|\|+ \| Sbjct 12 GPWKITVYDQENFQGKRMEFTSSC 35
Rana temporaria	Query 10 GHWRMVVWDEEGFQGRRHEFTAEC 33 \| \|++ \|+\|+\| \|\|\|+\| \|\|\|+ \| Sbjct 12 GPWKITVYDQENFQGKRMEFTSSC 35
Contains: Beta crystallin B1B	Query 10 GHWRMVVWDEEGFQGRRHEFTAEC 33 \| +\|+\|\|+++\| \|\|\|\|\| \|\|+ \|\| Sbjct 55 GSYRLVVFEQENFQGRRVEFSGEC 78
Ovis aries	Query 10 GHWRMVVWDEEGFQGRRHEFTAECP 34 \| ++++\|++ \| \|\|\|+\| \| \|\|\|\|\| Sbjct 22 GSYKVIVYEMENFQGKRCELTAECP 46
synthetic construct	Query 10 GHWRMVVWDEEGFQGRRHEFTAEC 33 \|++\|+\|\|++ \| \|\|\|\|\| \|\|+ \|\| Sbjct 57 GNYRLVVFELENFQGRRAEFSGEC 80
Sus scrofa	Query 10 GHWRMVVWDEEGFQGRRHEFTAEC 33 \| +++\|\|+++\| \|\|\|\|\| \|\|+ \|\| Sbjct 54 GSYKLVVFEQENFQGRRVEFSGEC 77
Canis familiaris	Query 10 GHWRMVVWDEEGFQGRRHEFTAEC 33 \| +++\|\|+++\| \|\|\|\|\| \|\|+ \|\| Sbjct 53 GSYKLVVFEQENFQGRRVEFSGEC 76

* References

[PubMed ID: 17470285] Pirity MK, Wang WL, Wolf LV, Tamm ER, Schreiber-Agus N, Cvekl A, Rybp, a polycomb complex-associated protein, is required for mouse eye development. BMC Dev Biol. 2007 Apr 30;7:39.

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[PubMed ID: 16162653] ... Yoshimoto A, Saigou Y, Higashi Y, Kondoh H, Regulation of ocular lens development by Smad-interacting protein 1 involving Foxe3 activation. Development. 2005 Oct;132(20):4437-48. Epub 2005 Sep 14.

[PubMed ID: 15593327] ... Min JN, Zhang Y, Moskophidis D, Mivechi NF, Unique contribution of heat shock transcription factor 4 in ocular lens development and fiber cell differentiation. Genesis. 2004 Dec;40(4):205-17.

[PubMed ID: 11836498] ... Suzuki T, Li W, Zhang Q, Karim A, Novak EK, Sviderskaya EV, Hill SP, Bennett DC, Levin AV, Nieuwenhuis HK, Fong CT, Castellan C, Miterski B, Swank RT, Spritz RA, Hermansky-Pudlak syndrome is caused by mutations in HPS4, the human homolog of the mouse light-ear gene. Nat Genet. 2002 Mar;30(3):321-4. Epub 2002 Feb 11.

[PubMed ID: 11714668] ... Dimanlig PV, Faber SC, Auerbach W, Makarenkova HP, Lang RA, The upstream ectoderm enhancer in Pax6 has an important role in lens induction. Development. 2001 Nov;128(22):4415-24.

[PubMed ID: 10595510] ... Gonzalez M, Ruggiero FP, Chang Q, Shi YJ, Rich MM, Kraner S, Balice-Gordon RJ, Disruption of Trkb-mediated signaling induces disassembly of postsynaptic receptor clusters at neuromuscular junctions. Neuron. 1999 Nov;24(3):567-83.

[PubMed ID: 9693140] ... Lovicu FJ, Overbeek PA, Overlapping effects of different members of the FGF family on lens fiber differentiation in transgenic mice. Development. 1998 Sep;125(17):3365-77.

[PubMed ID: 8812449] ... Bertuzzi S, Sheng HZ, Copeland NG, Gilbert DJ, Jenkins NA, Taira M, Dawid IB, Westphal H, Molecular cloning, structure, and chromosomal localization of the mouse LIM/homeobox gene Lhx5. Genomics. 1996 Sep 1;36(2):234-9.

[PubMed ID: 8575764] ... Hulsebos TJ, Gilbert DJ, Delattre O, Smink LJ, Dunham I, Westerveld A, Thomas G, Jenkins NA, Copeland NG, Assignment of the beta B1 crystallin gene (CRYBB1) to human chromosome 22 and mouse chromosome 5. Genomics. 1995 Oct 10;29(3):712-8.

[PubMed ID: 7789995] ... Hulsebos TJ, Jenkins NA, Gilbert DJ, Copeland NG, The beta crystallin genes on human chromosome 22 define a new region of homology with mouse chromosome 5. Genomics. 1995 Jan 20;25(2):574-6.