CaMPDB :: Substrate

XSB2018 : plasma membrane calcium ATPase 2 [Rattus norvegicus]

This entry is computationally expanded from SB0056

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	Plasma membrane calcium-transporting ATPase 2; PMCA2; 3.6.3.8; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; plasma membrane calcium ATPase 2; ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2; Na+K+ transporting; beta polypeptide 2; plasma membrane Ca++-ATPase
Gene Names	Atp2b2; ATPase, Ca++ transporting, plasma membrane 2
Gene Locus	4q41.3-q42.1; chromosome 4
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_036640	NM_012508	24215	P11506	N/A	N/A	N/A	rno:24215

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 1198 aa

Average Mass: 132.616 kDa

Monoisotopic Mass: 132.531 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Cation_ATPase_N 1.	41	124	72.9	1.1e-18
E1-E2_ATPase 1.	156	444	225.1	1.8e-64
Hydrolase 1.	448	796	55.8	1.7e-13
Cation_ATPase_C 1.	857	1039	210.7	3.8e-60

3. Sequence Information

Fasta Sequence: XSB2018.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites]

Cleavage sites (±10aa)

[Site 1] EIDHAERELR¹⁰⁷⁹-RGQILWFRGL

Arg¹⁰⁷⁹ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Glu¹⁰⁷⁰	Ile¹⁰⁷¹	Asp¹⁰⁷²	His¹⁰⁷³	Ala¹⁰⁷⁴	Glu¹⁰⁷⁵	Arg¹⁰⁷⁶	Glu¹⁰⁷⁷	Leu¹⁰⁷⁸	Arg¹⁰⁷⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹⁰⁸⁰	Gly¹⁰⁸¹	Gln¹⁰⁸²	Ile¹⁰⁸³	Leu¹⁰⁸⁴	Trp¹⁰⁸⁵	Phe¹⁰⁸⁶	Arg¹⁰⁸⁷	Gly¹⁰⁸⁸	Leu¹⁰⁸⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
AGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEG

Summary

#	organism	max score	hits	top seq
1	Canis familiaris	89.00	102	PREDICTED: similar to plasma membrane calcium ATPa
2	N/A	89.00	41	-
3	Homo sapiens	89.00	27	plasma membrane calcium ATPase 2 isoform 2
4	Mus musculus	89.00	12	plasma membrane Ca2+-ATPase isoform 2
5	Bos taurus	89.00	12	PREDICTED: similar to plasma membrane calcium ATPa
6	Pan troglodytes	89.00	11	PREDICTED: plasma membrane calcium ATPase 2 isofor
7	Macaca mulatta	89.00	7	PREDICTED: plasma membrane calcium ATPase 2, parti
8	Rattus norvegicus	89.00	6	ATPase, Ca++ transporting, plasma membrane 2
9	Gallus gallus	87.80	9	PREDICTED: similar to plasma membrane calcium ATPa
10	Tetraodon nigroviridis	85.50	4	unnamed protein product
11	Danio rerio	85.50	4	PREDICTED: hypothetical protein, partial
12	Monodelphis domestica	82.00	3	PREDICTED: similar to plasma membrane calcium ATPa
13	Sus scrofa	82.00	2	plasma membrane calcium ATPase 1
14	Rana catesbeiana	80.10	2	PMCA1bx
15	Oryctolagus cuniculus	73.20	1	Ca2+/Mg2+ ATPase
16	synthetic construct	71.60	2	hypothetical protein
17	Xenopus laevis	70.50	1	ATPase, Ca++ transporting, plasma membrane 3
18	rats, ROS 17/2.8 osteosarcoma cells, Peptide Partial, 123 aa	68.90	1	plasma membrane Ca(2+)-ATPase PMCA1b isoform {alte
19	Pongo pygmaeus	66.60	1	hypothetical protein
20	Procambarus clarkii	66.20	1	plasma membrane calcium ATPase PMCA3

Top-ranked sequences

organism	matching
Canis familiaris	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
N/A	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Homo sapiens	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Mus musculus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Bos taurus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Pan troglodytes	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1056 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1115
Macaca mulatta	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 542 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 601
Rattus norvegicus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1050 AGRLTQKEEIPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109
Gallus gallus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|\|+\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1052 AGRLTEKEEVPEEELNEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1111
Tetraodon nigroviridis	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 288 AGQLTQKDELPEEDVNEENEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 347
Danio rerio	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 293 AGQLTQKDEMPEEEMNEDQEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 352
Monodelphis domestica	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1059 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1118
Sus scrofa	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1072 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
Rana catesbeiana	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\| \| \| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1066 AGHGTHKDEIPEEELAEDAEEIDHAERELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 1125
Oryctolagus cuniculus	Query 1054 TQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\| \| \|\|\|\|\|\|\|#\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1076 TQKREIGEEELAEDVEEIVHAERELR#RWQILWFRGLNRIQTQIRVVNAFRSSLYEG 1131
synthetic construct	Query 1071 IDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYE 1108 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| \|\|\|+\| Sbjct 1079 IDHAEMELR#RGQILWFRGLNRIQTQIKVVKAFHSSLHE 1116
Xenopus laevis	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRS 1104 \|\| \|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| \|+\| Sbjct 1098 AGNLTQKEENQEEELNEDAEEIDHAERELR#RGQILWFRGLNRIQTQIEVVNTFKS 1152
rats, ROS 17/2.8 osteosarcoma cells, Peptide Partial, 123 aa	Query 1076 RELR#RGQILWFRGLNRIQTQIRVVKAFRSSLYEG 1109 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| Sbjct 1 RELR#RGQILWFRGLNRIQTQIRVVNAFRSSLYEG 34
Pongo pygmaeus	Query 1050 AGRLTQKXXXXXXXXXXXXXXIDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRS 1104 \|\| \|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ \|\| \|\|+\| Sbjct 150 AGHGTQKEEIPEEELAEDVEEIDHAERELR#RGQILWFRGLNRIQTQMDVVNAFQS 204
Procambarus clarkii	Query 1071 IDHAERELR#RGQILWFRGLNRIQTQIRVVKAFRS 1104 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\| \|\|+\| Sbjct 1119 IDHAERELR#RGQILWFRGLNRIQTQIEVVNAFKS 1152

* References

[PubMed ID: 19025983] Burette AC, Strehler EE, Weinberg RJ, "Fast" plasma membrane calcium pump PMCA2a concentrates in GABAergic terminals in the adult rat brain. J Comp Neurol. 2009 Feb 1;512(4):500-13.

» More (9) « Hide Below

[PubMed ID: 19120150] ... Zylinska L, Kowalska I, Kozaczuk A, Fast action of neuroactive steroids on plasma membrane calcium pump in PC12 cells. Ann N Y Acad Sci. 2008 Dec;1148:515-9.

[PubMed ID: 18643776] ... Kamagate A, Sener A, Courtois P, Malaisse WJ, Herchuelz A, Effects of plasma membrane Ca(2+)-ATPase overexpression upon D-glucose metabolism in insulin-producing BRIN-BD11 cells. Biosci Rep. 2008 Oct;28(5):251-8.

[PubMed ID: 17970729] ... Castillo K, Delgado R, Bacigalupo J, Plasma membrane Ca(2+)-ATPase in the cilia of olfactory receptor neurons: possible role in Ca(2+) clearance. Eur J Neurosci. 2007 Nov;26(9):2524-31. Epub 2007 Oct 23.

[PubMed ID: 17183553] ... Burette A, Weinberg RJ, Perisynaptic organization of plasma membrane calcium pumps in cerebellar cortex. J Comp Neurol. 2007 Feb 20;500(6):1127-35.

[PubMed ID: 16554037] ... Sgambato-Faure V, Xiong Y, Berke JD, Hyman SE, Strehler EE, The Homer-1 protein Ania-3 interacts with the plasma membrane calcium pump. Biochem Biophys Res Commun. 2006 May 5;343(2):630-7. Epub 2006 Mar 15.

[PubMed ID: 15060019] ... Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ, High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence. Genome Res. 2004 Apr;14(4):750-7.

[PubMed ID: 8428948] ... Keeton TP, Burk SE, Shull GE, Alternative splicing of exons encoding the calmodulin-binding domains and C termini of plasma membrane Ca(2+)-ATPase isoforms 1, 2, 3, and 4. J Biol Chem. 1993 Feb 5;268(4):2740-8.

[PubMed ID: 1315513] ... Adamo HP, Penniston JT, New Ca2+ pump isoforms generated by alternative splicing of rPMCA2 mRNA. Biochem J. 1992 Apr 15;283 ( Pt 2):355-9.

[PubMed ID: 2837461] ... Shull GE, Greeb J, Molecular cloning of two isoforms of the plasma membrane Ca2+-transporting ATPase from rat brain. Structural and functional domains exhibit similarity to Na+,K+- and other cation transport ATPases. J Biol Chem. 1988 Jun 25;263(18):8646-57.