XSB2076 : Beta-crystallin B3
[ CaMP Format ]
This entry is computationally expanded from SB0095
* Basic Information
| Organism | Rattus norvegicus (Norway rat) |
| Protein Names | Beta-B3 crystallin |
| Gene Names | Crybb3 |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 211 aa
Average Mass: 24.334 kDa
Monoisotopic Mass: 24.319 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| --- cleavage 5 --- | ||||
| --- cleavage 17 --- | ||||
| --- cleavage 15 --- | ||||
| --- cleavage 10 --- | ||||
| Crystall 1. | 25 | 107 | 154.7 | 2.9e-43 |
| Crystall 2. | 115 | 197 | 160.0 | 6.9e-45 |
3. Sequence Information
Fasta Sequence: XSB2076.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
4 [sites]
Cleavage sites (±10aa)
[Site 1] MAEQH5-GAPEQAAASK
His5 
Gly
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| - | - | - | - | - | Met1 | Ala2 | Glu3 | Gln4 | His5 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gly6 | Ala7 | Pro8 | Glu9 | Gln10 | Ala11 | Ala12 | Ala13 | Ser14 | Lys15 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MAEQHGAPEQAAASKSHGGLGGSYKVTVYELENFQ |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Rattus norvegicus | 74.70 | 2 | AF287304_1 betaB3-crystallin |
| 2 | N/A | 74.70 | 1 | C |
| 3 | Mus musculus | 73.20 | 1 | crystallin, beta B3 |
| 4 | Cavia porcellus | 71.60 | 1 | betaB3-crystallin |
| 5 | Macaca mulatta | 70.90 | 2 | PREDICTED: crystallin, beta B2 isoform 1 |
| 6 | Pan troglodytes | 69.30 | 2 | PREDICTED: crystallin, beta B3 isoform 1 |
| 7 | Ovis aries | 67.80 | 1 | crystallin, beta B3 |
| 8 | Homo sapiens | 67.00 | 4 | Crystallin, beta B3 |
| 9 | Bos taurus | 66.20 | 1 | crystallin, beta B3 |
| 10 | Xenopus laevis | 58.50 | 2 | MGC84109 protein |
| 11 | Tetraodon nigroviridis | 48.10 | 1 | unnamed protein product |
| 12 | Danio rerio | 46.20 | 1 | beta B3-crystallin |
| 13 | Gallus gallus | 45.10 | 1 | crystallin, beta B3 |
| 14 | Monodelphis domestica | 43.10 | 1 | PREDICTED: similar to betaB2-crystallin |
Top-ranked sequences
| organism | matching |
|---|---|
| Rattus norvegicus | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#|||||||||||||||||||||||||||||| Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |
| N/A | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#|||||||||||||||||||||||||||||| Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |
| Mus musculus | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#|||||||| ||||||||||||||||||||| Sbjct 1 MAEQH#GAPEQAAAGKSHGGLGGSYKVTVYELENFQ 35 |
| Cavia porcellus | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#|||||||| |||||||| |||||||||||| Sbjct 1 MAEQH#GAPEQAAAGKSHGGLGGGYKVTVYELENFQ 35 |
| Macaca mulatta | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#|||||||| |||||||||||| |||||||| Sbjct 1 MAEQH#GAPEQAAAGKSHGGLGGSYKVIVYELENFQ 35 |
| Pan troglodytes | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#| |||||| |||||||||||| |||||||| Sbjct 1 MAEQH#GTPEQAAAGKSHGGLGGSYKVIVYELENFQ 35 |
| Ovis aries | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||# ||||||| |||||||||||| |||+|||| Sbjct 1 MAEQH#SAPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35 |
| Homo sapiens | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||#|||||||| |||| ||||||| +||||||| Sbjct 1 MAEQH#GAPEQAAAGKSHGDLGGSYKVILYELENFQ 35 |
| Bos taurus | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |||||# |||||| |||||||||||| |||+|||| Sbjct 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35 |
| Xenopus laevis | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |+| # |||| || |||||+||+||+|+||||||| Sbjct 1 MSELQ#SAPEQMAAGKSHGGIGGNYKITLYELENFQ 35 |
| Tetraodon nigroviridis | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |+|+ # |||| || || || | +||| ++| |||| Sbjct 33 MSERQ#SAPEQLAAGKSQGGAGATYKVLLFEFENFQ 67 |
| Danio rerio | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 |++| #| |+| | || || | |||++|| |||+ Sbjct 1 MSDQQ#GVPDQQVAGKSQGGAGAIYKVSLYEFENFR 35 |
| Gallus gallus | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35 | || # ||| + | ||+||+|+||||||| Sbjct 1 MTEQQ#SPPEQMVTGEGAGERGGNYKITIYELENFQ 35 |
| Monodelphis domestica | Query 1 MAEQH#GAPEQAAASKSHGGLGGSYK 25 | || # |||||| |||||||||+| Sbjct 125 MMEQQ#SVPEQAAAGKSHGGLGGSFK 149 |
[Site 2] PEQAAASKSH17-GGLGGSYKVT
His17 Gly
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Pro8 | Glu9 | Gln10 | Ala11 | Ala12 | Ala13 | Ser14 | Lys15 | Ser16 | His17 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gly18 | Gly19 | Leu20 | Gly21 | Gly22 | Ser23 | Tyr24 | Lys25 | Val26 | Thr27 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MAEQHGAPEQAAASKSHGGLGGSYKVTVYELENFQGKRCELSAECPN |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | N/A | 101.00 | 14 | C |
| 2 | Rattus norvegicus | 100.00 | 8 | crystallin, beta B3 |
| 3 | Mus musculus | 99.80 | 10 | crystallin, beta B3 |
| 4 | Cavia porcellus | 98.20 | 3 | betaB3-crystallin |
| 5 | Pan troglodytes | 94.00 | 6 | PREDICTED: crystallin, beta B3 isoform 1 |
| 6 | Macaca mulatta | 93.60 | 5 | PREDICTED: crystallin, beta B2 isoform 1 |
| 7 | Ovis aries | 93.20 | 1 | crystallin, beta B3 |
| 8 | Homo sapiens | 91.70 | 8 | CRBB3_HUMAN Beta crystallin B3 (Beta-B3-crystallin |
| 9 | Bos taurus | 91.70 | 6 | crystallin, beta B3 |
| 10 | Xenopus laevis | 78.20 | 13 | MGC84109 protein |
| 11 | Gallus gallus | 64.70 | 8 | crystallin, beta B3 |
| 12 | Danio rerio | 62.80 | 18 | beta B3-crystallin |
| 13 | Tetraodon nigroviridis | 58.20 | 9 | unnamed protein product |
| 14 | Xenopus tropicalis | 45.10 | 2 | Unknown (protein for MGC:122148) |
| 15 | Monodelphis domestica | 43.10 | 4 | PREDICTED: similar to betaB2-crystallin |
| 16 | Canis lupus familiaris | 43.10 | 3 | crystallin, beta A1 |
| 17 | Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form | 42.00 | 1 | CRBA1_BOVIN Beta crystallin A3 |
| 18 | Oryctolagus cuniculus | 41.60 | 2 | beta A3-crystallin |
| 19 | Canis familiaris | 41.60 | 1 | PREDICTED: similar to crystallin, beta B1 |
| 20 | Sus scrofa | 41.60 | 1 | beta crystallin subunit beta B1 |
| 21 | synthetic construct | 41.60 | 1 | crystallin beta B1 |
| 22 | Dicentrarchus labrax | 40.40 | 1 | crystallin beta B1 |
| 23 | Contains: Beta crystallin B1B | 39.30 | 1 | CRBB1_RAT Beta crystallin B1 |
| 24 | Rana catesbeiana | 38.90 | 4 | CRB32_RANCA Beta crystallin A3-2 emb |
| 25 | Rana temporaria | 38.90 | 2 | unnamed protein product |
| 26 | Cynops pyrrhogaster | 35.40 | 3 | newt beta B1-crystallin |
| 27 | Petenia splendida x Cichlasoma synspilum | 35.40 | 1 | AAA50959.1 |
| 28 | Macropus fuliginosus | 34.30 | 1 | betaA2-crystallin |
| 29 | Iguana iguana | 32.30 | 1 | betaA2-crystallin |
Top-ranked sequences
| organism | matching |
|---|---|
| N/A | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |||||||||||||||||#|||||||||||||||||||||||||||||| Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |
| Rattus norvegicus | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |||||||||||||+|||#|||||||||||||||||||||||||||||| Sbjct 1 MAEQHGAPEQAAANKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |
| Mus musculus | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 ||||||||||||| |||#|||||||||||||||||||||||||||||| Sbjct 1 MAEQHGAPEQAAAGKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |
| Cavia porcellus | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 ||||||||||||| |||#||||| |||||||||||||||||||||||| Sbjct 1 MAEQHGAPEQAAAGKSH#GGLGGGYKVTVYELENFQGKRCELSAECPN 47 |
| Pan troglodytes | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |||||| |||||| |||#||||||||| |||||||||||||||||||+ Sbjct 1 MAEQHGTPEQAAAGKSH#GGLGGSYKVIVYELENFQGKRCELSAECPS 47 |
| Macaca mulatta | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 ||||||||||||| |||#||||||||| |||||||||| ||||||||+ Sbjct 1 MAEQHGAPEQAAAGKSH#GGLGGSYKVIVYELENFQGKHCELSAECPS 47 |
| Ovis aries | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 ||||| ||||||| |||#||||||||| |||+||||||||||+||||| Sbjct 1 MAEQHSAPEQAAAGKSH#GGLGGSYKVIVYEMENFQGKRCELTAECPN 47 |
| Homo sapiens | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 ||||||||||||| |||#| ||||||| +||||||||||||||||||+ Sbjct 1 MAEQHGAPEQAAAGKSH#GDLGGSYKVILYELENFQGKRCELSAECPS 47 |
| Bos taurus | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 ||||| |||||| |||#||||||||| |||+||||||||||+||||| Sbjct 1 MAEQHSTPEQAAAGKSH#GGLGGSYKVIVYEMENFQGKRCELTAECPN 47 |
| Xenopus laevis | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 |+| |||| || |||#||+||+||+|+|||||||||+|||+ || | Sbjct 1 MSELQSAPEQMAAGKSH#GGIGGNYKITLYELENFQGKKCELTGECKN 47 |
| Gallus gallus | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 | || ||| + #| ||+||+|+||||||||+||||| | || Sbjct 1 MTEQQSPPEQMVTGEGA#GERGGNYKITIYELENFQGRRCELSEELPN 47 |
| Danio rerio | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAEC 45 |++| | |+| | || #|| | |||++|| |||+||+ |||||| Sbjct 1 MSDQQGVPDQQVAGKSQ#GGAGAIYKVSLYEFENFRGKKLELSAEC 45 |
| Tetraodon nigroviridis | Query 1 MAEQHGAPEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAEC 45 |+|+ |||| || || #|| | +||| ++| ||||| + | |++| Sbjct 33 MSERQSAPEQLAAGKSQ#GGAGATYKVLLFEFENFQGCKAEFSSDC 77 |
| Xenopus tropicalis | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 ||+|+|||+ ||||||| | |+ ||| Sbjct 12 GSWKITVYDQENFQGKRMEFSSSCPN 37 |
| Monodelphis domestica | Query 23 SYKVTVYELENFQGKRCELSAECPN 47 | +|+++||||||||+|+|| |||| Sbjct 215 SLQVSIFELENFQGKKCDLSTECPN 239 Query 1 MAEQHGAPEQAAASKSH#GGLGGSYK 25 | || |||||| |||#||||||+| Sbjct 125 MMEQQSVPEQAAAGKSH#GGLGGSFK 149 |
| Canis lupus familiaris | Query 19 GLGGSYKVTVYELENFQGKRCELSAECPN 47 | | +|+|+|+ ||||||| | ++ ||| Sbjct 26 GSAGPWKITIYDQENFQGKRMEFTSSCPN 54 |
| Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form | Query 19 GLGGSYKVTVYELENFQGKRCELSAECPN 47 | | +|+|+|+ ||||||| | ++ ||| Sbjct 26 GSVGPWKITIYDQENFQGKRMEFTSSCPN 54 |
| Oryctolagus cuniculus | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 | +|+|+|+ ||||||| | ++ ||| Sbjct 29 GPWKITIYDQENFQGKRMEFTSSCPN 54 |
| Canis familiaris | Query 7 APEQAAASKSH#GGLGGSYKVTVYELENFQGKRCELSAECPN 47 || +|+ # ||||+ |+| |||||+| | | || | Sbjct 38 APATVPTTKAG#DLPPGSYKLVVFEQENFQGRRVEFSGECLN 78 |
| Sus scrofa | Query 8 PEQAAASKSH#GGLG----GSYKVTVYELENFQGKRCELSAECPN 47 | || | # | ||||+ |+| |||||+| | | || | Sbjct 36 PAQAPAQPMP#AAKGDLPPGSYKLVVFEQENFQGRRVEFSGECLN 79 |
| synthetic construct | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 |+|++ |+|||||||+| | | || | Sbjct 57 GNYRLVVFELENFQGRRAEFSGECSN 82 |
| Dicentrarchus labrax | Query 2 AEQHGAPEQAAASKSH--#GGLG-GSYKVTVYELENFQGKRCELSAECPN 47 |+ ||| ||+||+ #| | |||++ +++ |||||+ |+ || | Sbjct 15 AKDKGAPAPAASSKATKT#GEPGMGSYRIMMFDQENFQGRMIEVQNECMN 63 |
| Contains: Beta crystallin B1B | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 |||++ |+| |||||+| | | || | Sbjct 55 GSYRLVVFEQENFQGRRVEFSGECLN 80 |
| Rana catesbeiana | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 | +|+|||+ ||||||| | ++ | | Sbjct 29 GPWKITVYDQENFQGKRMEFTSSCAN 54 |
| Rana temporaria | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 | +|+|||+ ||||||| | ++ | | Sbjct 12 GPWKITVYDQENFQGKRMEFTSSCAN 37 |
| Cynops pyrrhogaster | Query 2 AEQHGAPEQAAASKSH---#GGLGGSYKVTVYELENFQGKRCELSAEC 45 +++ | | || + # |+||+ ++| |||||+ || || Sbjct 23 SKEKGVPPSVPISKPNKAG#DSSVGTYKIIIFEQENFQGRHLELVNEC 69 |
| Petenia splendida x Cichlasoma synspilum | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 |||++ +|| |||||+ || +| | Sbjct 86 GSYRMRIYERENFQGQNHELMNDCDN 111 |
| Macropus fuliginosus | Query 25 KVTVYELENFQGKRCELSAEC 45 ++|++| |||||+|||| ++| Sbjct 13 RLTLWEEENFQGRRCELMSDC 33 |
| Iguana iguana | Query 22 GSYKVTVYELENFQGKRCELSAECPN 47 | +|+||+| |||||+||| |||+ Sbjct 3 GQFKITVWEEENFQGERCEFLMECPS 28 Query 25 KVTVYELENFQGKRCELSAECPN 47 |||+|| |||||++ ||| + |+ Sbjct 99 KVTLYEGENFQGRKFELSDDYPS 121 |
[Site 3] GAPEQAAASK15-SHGGLGGSYK
Lys15 Ser
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Gly6 | Ala7 | Pro8 | Glu9 | Gln10 | Ala11 | Ala12 | Ala13 | Ser14 | Lys15 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ser16 | His17 | Gly18 | Gly19 | Leu20 | Gly21 | Gly22 | Ser23 | Tyr24 | Lys25 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MAEQHGAPEQAAASKSHGGLGGSYKVTVYELENFQGKRCELSAEC |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | N/A | 96.30 | 9 | C |
| 2 | Rattus norvegicus | 95.10 | 7 | crystallin, beta B3 |
| 3 | Mus musculus | 94.70 | 6 | crystallin, beta B3 |
| 4 | Cavia porcellus | 93.20 | 3 | betaB3-crystallin |
| 5 | Pan troglodytes | 90.90 | 5 | PREDICTED: crystallin, beta B3 isoform 1 |
| 6 | Macaca mulatta | 90.50 | 3 | PREDICTED: crystallin, beta B2 isoform 1 |
| 7 | Homo sapiens | 88.60 | 6 | Crystallin, beta B3 |
| 8 | Ovis aries | 88.20 | 1 | crystallin, beta B3 |
| 9 | Bos taurus | 86.70 | 4 | crystallin, beta B3 |
| 10 | Xenopus laevis | 76.30 | 8 | MGC84109 protein |
| 11 | Danio rerio | 62.80 | 15 | beta B3-crystallin |
| 12 | Gallus gallus | 60.10 | 3 | crystallin, beta B3 |
| 13 | Tetraodon nigroviridis | 58.20 | 7 | unnamed protein product |
| 14 | Sus scrofa | 40.40 | 1 | beta crystallin subunit beta B1 |
| 15 | Canis familiaris | 40.40 | 1 | PREDICTED: similar to crystallin, beta B1 |
| 16 | Monodelphis domestica | 40.00 | 3 | PREDICTED: similar to betaB2-crystallin |
| 17 | Xenopus tropicalis | 40.00 | 2 | Unknown (protein for MGC:122148) |
| 18 | synthetic construct | 39.70 | 1 | crystallin beta B1 |
| 19 | Dicentrarchus labrax | 38.90 | 1 | crystallin beta B1 |
| 20 | Iguana iguana | 38.90 | 1 | betaA2-crystallin |
| 21 | Canis lupus familiaris | 38.10 | 1 | crystallin, beta A1 |
| 22 | Contains: Beta crystallin B1B | 38.10 | 1 | CRBB1_RAT Beta crystallin B1 |
| 23 | Rana catesbeiana | 37.00 | 4 | CRB31_RANCA Beta crystallin A3-1 emb |
| 24 | Rana temporaria | 37.00 | 2 | CRBA1_RANTE Beta crystallin A1 emb |
| 25 | Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form | 37.00 | 1 | CRBA1_BOVIN Beta crystallin A3 |
| 26 | Oryctolagus cuniculus | 36.60 | 2 | beta A3-crystallin |
| 27 | Cynops pyrrhogaster | 35.40 | 1 | newt beta B1-crystallin |
| 28 | Macropus fuliginosus | 34.30 | 1 | betaA2-crystallin |
| 29 | Petenia splendida x Cichlasoma synspilum | 33.50 | 1 | AAA50959.1 |
Top-ranked sequences
| organism | matching |
|---|---|
| N/A | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |||||||||||||||#|||||||||||||||||||||||||||||| Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |
| Rattus norvegicus | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |||||||||||||+|#|||||||||||||||||||||||||||||| Sbjct 1 MAEQHGAPEQAAANK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |
| Mus musculus | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 ||||||||||||| |#|||||||||||||||||||||||||||||| Sbjct 1 MAEQHGAPEQAAAGK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |
| Cavia porcellus | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 ||||||||||||| |#||||||| |||||||||||||||||||||| Sbjct 1 MAEQHGAPEQAAAGK#SHGGLGGGYKVTVYELENFQGKRCELSAEC 45 |
| Pan troglodytes | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |||||| |||||| |#||||||||||| |||||||||||||||||| Sbjct 1 MAEQHGTPEQAAAGK#SHGGLGGSYKVIVYELENFQGKRCELSAEC 45 |
| Macaca mulatta | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 ||||||||||||| |#||||||||||| |||||||||| ||||||| Sbjct 1 MAEQHGAPEQAAAGK#SHGGLGGSYKVIVYELENFQGKHCELSAEC 45 |
| Homo sapiens | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 ||||||||||||| |#||| ||||||| +||||||||||||||||| Sbjct 1 MAEQHGAPEQAAAGK#SHGDLGGSYKVILYELENFQGKRCELSAEC 45 |
| Ovis aries | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 ||||| ||||||| |#||||||||||| |||+||||||||||+||| Sbjct 1 MAEQHSAPEQAAAGK#SHGGLGGSYKVIVYEMENFQGKRCELTAEC 45 |
| Bos taurus | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 ||||| |||||| |#||||||||||| |||+||||||||||+||| Sbjct 1 MAEQHSTPEQAAAGK#SHGGLGGSYKVIVYEMENFQGKRCELTAEC 45 |
| Xenopus laevis | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |+| |||| || |#||||+||+||+|+|||||||||+|||+ || Sbjct 1 MSELQSAPEQMAAGK#SHGGIGGNYKITLYELENFQGKKCELTGEC 45 |
| Danio rerio | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |++| | |+| | |#| || | |||++|| |||+||+ |||||| Sbjct 1 MSDQQGVPDQQVAGK#SQGGAGAIYKVSLYEFENFRGKKLELSAEC 45 |
| Gallus gallus | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAE 44 | || ||| +# | ||+||+|+||||||||+||||| | Sbjct 1 MTEQQSPPEQMVTGE#GAGERGGNYKITIYELENFQGRRCELSEE 44 |
| Tetraodon nigroviridis | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 |+|+ |||| || |#| || | +||| ++| ||||| + | |++| Sbjct 33 MSERQSAPEQLAAGK#SQGGAGATYKVLLFEFENFQGCKAEFSSDC 77 |
| Sus scrofa | Query 8 PEQAAASK#SHGGLG----GSYKVTVYELENFQGKRCELSAEC 45 | || | # | ||||+ |+| |||||+| | | || Sbjct 36 PAQAPAQP#MPAAKGDLPPGSYKLVVFEQENFQGRRVEFSGEC 77 |
| Canis familiaris | Query 7 APEQAAASK#SHGGLGGSYKVTVYELENFQGKRCELSAEC 45 || +|#+ ||||+ |+| |||||+| | | || Sbjct 38 APATVPTTK#AGDLPPGSYKLVVFEQENFQGRRVEFSGEC 76 |
| Monodelphis domestica | Query 1 MAEQHGAPEQAAASK#SHGGLGGSYK 25 | || |||||| |#||||||||+| Sbjct 125 MMEQQSVPEQAAAGK#SHGGLGGSFK 149 Query 23 SYKVTVYELENFQGKRCELSAEC 45 | +|+++||||||||+|+|| || Sbjct 215 SLQVSIFELENFQGKKCDLSTEC 237 |
| Xenopus tropicalis | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 ||+|+|||+ ||||||| | |+ | Sbjct 12 GSWKITVYDQENFQGKRMEFSSSC 35 |
| synthetic construct | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 |+|++ |+|||||||+| | | || Sbjct 57 GNYRLVVFELENFQGRRAEFSGEC 80 |
| Dicentrarchus labrax | Query 2 AEQHGAPEQAAASK#SH--GGLG-GSYKVTVYELENFQGKRCELSAEC 45 |+ ||| ||+||#+ | | |||++ +++ |||||+ |+ || Sbjct 15 AKDKGAPAPAASSK#ATKTGEPGMGSYRIMMFDQENFQGRMIEVQNEC 61 |
| Iguana iguana | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 | +|+||+| |||||+||| || Sbjct 3 GQFKITVWEEENFQGERCEFLMEC 26 |
| Canis lupus familiaris | Query 19 GLGGSYKVTVYELENFQGKRCELSAEC 45 | | +|+|+|+ ||||||| | ++ | Sbjct 26 GSAGPWKITIYDQENFQGKRMEFTSSC 52 |
| Contains: Beta crystallin B1B | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 |||++ |+| |||||+| | | || Sbjct 55 GSYRLVVFEQENFQGRRVEFSGEC 78 |
| Rana catesbeiana | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 | +|+|||+ ||||||| | ++ | Sbjct 29 GPWKITVYDQENFQGKRMEFTSSC 52 |
| Rana temporaria | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 | +|+|||+ ||||||| | ++ | Sbjct 12 GPWKITVYDQENFQGKRMEFTSSC 35 |
| Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form | Query 19 GLGGSYKVTVYELENFQGKRCELSAEC 45 | | +|+|+|+ ||||||| | ++ | Sbjct 26 GSVGPWKITIYDQENFQGKRMEFTSSC 52 |
| Oryctolagus cuniculus | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 | +|+|+|+ ||||||| | ++ | Sbjct 29 GPWKITIYDQENFQGKRMEFTSSC 52 |
| Cynops pyrrhogaster | Query 2 AEQHGAPEQAAASK#SH---GGLGGSYKVTVYELENFQGKRCELSAEC 45 +++ | | ||# + |+||+ ++| |||||+ || || Sbjct 23 SKEKGVPPSVPISK#PNKAGDSSVGTYKIIIFEQENFQGRHLELVNEC 69 |
| Macropus fuliginosus | Query 25 KVTVYELENFQGKRCELSAEC 45 ++|++| |||||+|||| ++| Sbjct 13 RLTLWEEENFQGRRCELMSDC 33 |
| Petenia splendida x Cichlasoma synspilum | Query 22 GSYKVTVYELENFQGKRCELSAEC 45 |||++ +|| |||||+ || +| Sbjct 86 GSYRMRIYERENFQGQNHELMNDC 109 |
[Site 4] MAEQHGAPEQ10-AAASKSHGGL
Gln10 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Met1 | Ala2 | Glu3 | Gln4 | His5 | Gly6 | Ala7 | Pro8 | Glu9 | Gln10 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala11 | Ala12 | Ala13 | Ser14 | Lys15 | Ser16 | His17 | Gly18 | Gly19 | Leu20 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MAEQHGAPEQAAASKSHGGLGGSYKVTVYELENFQGKRCE |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | N/A | 86.30 | 7 | C |
| 2 | Rattus norvegicus | 86.30 | 4 | AF287304_1 betaB3-crystallin |
| 3 | Mus musculus | 84.70 | 3 | crystallin, beta B3 |
| 4 | Cavia porcellus | 83.20 | 3 | betaB3-crystallin |
| 5 | Pan troglodytes | 80.90 | 5 | PREDICTED: crystallin, beta B3 isoform 1 |
| 6 | Macaca mulatta | 80.50 | 3 | PREDICTED: crystallin, beta B2 isoform 1 |
| 7 | Ovis aries | 79.30 | 1 | crystallin, beta B3 |
| 8 | Homo sapiens | 78.60 | 6 | CRBB3_HUMAN Beta crystallin B3 (Beta-B3-crystallin |
| 9 | Bos taurus | 77.80 | 4 | crystallin, beta B3 |
| 10 | Xenopus laevis | 68.90 | 5 | MGC84109 protein |
| 11 | Gallus gallus | 55.50 | 3 | crystallin, beta B3 |
| 12 | Danio rerio | 52.80 | 6 | beta B3-crystallin |
| 13 | Tetraodon nigroviridis | 52.00 | 1 | unnamed protein product |
| 14 | Monodelphis domestica | 43.10 | 2 | PREDICTED: similar to betaB2-crystallin |
| 15 | Iguana iguana | 34.70 | 1 | betaA2-crystallin |
| 16 | Xenopus tropicalis | 34.70 | 1 | Unknown (protein for MGC:122148) |
| 17 | Dicentrarchus labrax | 33.90 | 1 | crystallin beta B1 |
| 18 | Canis lupus familiaris | 33.90 | 1 | crystallin, beta A1 |
| 19 | Canis familiaris | 33.50 | 1 | PREDICTED: similar to crystallin, beta B1 |
| 20 | Sus scrofa | 33.50 | 1 | beta crystallin subunit beta B1 |
| 21 | Rana catesbeiana | 32.70 | 4 | CRB31_RANCA Beta crystallin A3-1 emb |
| 22 | Rana temporaria | 32.70 | 2 | unnamed protein product |
| 23 | synthetic construct | 32.70 | 1 | crystallin beta B1 |
| 24 | Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form | 32.70 | 1 | CRBA1_BOVIN Beta crystallin A3 |
| 25 | Oryctolagus cuniculus | 32.30 | 2 | beta A3-crystallin |
Top-ranked sequences
| organism | matching |
|---|---|
| N/A | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||||||||#|||||||||||||||||||||||||||||| Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 |
| Rattus norvegicus | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||||||||#|||||||||||||||||||||||||||||| Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 |
| Mus musculus | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||||||||#||| |||||||||||||||||||||||||| Sbjct 1 MAEQHGAPEQ#AAAGKSHGGLGGSYKVTVYELENFQGKRCE 40 |
| Cavia porcellus | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||||||||#||| |||||||| ||||||||||||||||| Sbjct 1 MAEQHGAPEQ#AAAGKSHGGLGGGYKVTVYELENFQGKRCE 40 |
| Pan troglodytes | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 |||||| |||#||| |||||||||||| ||||||||||||| Sbjct 1 MAEQHGTPEQ#AAAGKSHGGLGGSYKVIVYELENFQGKRCE 40 |
| Macaca mulatta | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||||||||#||| |||||||||||| |||||||||| || Sbjct 1 MAEQHGAPEQ#AAAGKSHGGLGGSYKVIVYELENFQGKHCE 40 |
| Ovis aries | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||| ||||#||| |||||||||||| |||+||||||||| Sbjct 1 MAEQHSAPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40 |
| Homo sapiens | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||||||||#||| |||| ||||||| +|||||||||||| Sbjct 1 MAEQHGAPEQ#AAAGKSHGDLGGSYKVILYELENFQGKRCE 40 |
| Bos taurus | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 ||||| |||#||| |||||||||||| |||+||||||||| Sbjct 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40 |
| Xenopus laevis | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 |+| ||||# || |||||+||+||+|+|||||||||+|| Sbjct 1 MSELQSAPEQ#MAAGKSHGGIGGNYKITLYELENFQGKKCE 40 |
| Gallus gallus | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 | || |||# + | ||+||+|+||||||||+||| Sbjct 1 MTEQQSPPEQ#MVTGEGAGERGGNYKITIYELENFQGRRCE 40 |
| Danio rerio | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 |++| | |+|# | || || | |||++|| |||+||+ | Sbjct 1 MSDQQGVPDQ#QVAGKSQGGAGAIYKVSLYEFENFRGKKLE 40 |
| Tetraodon nigroviridis | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 |+|+ ||||# || || || | +||| ++| ||||| + | Sbjct 33 MSERQSAPEQ#LAAGKSQGGAGATYKVLLFEFENFQGCKAE 72 |
| Monodelphis domestica | Query 1 MAEQHGAPEQ#AAASKSHGGLGGSYK 25 | || |||#||| |||||||||+| Sbjct 125 MMEQQSVPEQ#AAAGKSHGGLGGSFK 149 |
| Iguana iguana | Query 22 GSYKVTVYELENFQGKRCE 40 | +|+||+| |||||+||| Sbjct 3 GQFKITVWEEENFQGERCE 21 |
| Xenopus tropicalis | Query 22 GSYKVTVYELENFQGKRCE 40 ||+|+|||+ ||||||| | Sbjct 12 GSWKITVYDQENFQGKRME 30 |
| Dicentrarchus labrax | Query 2 AEQHGAPEQ#AAASKSH--GGLG-GSYKVTVYELENFQGKRCE 40 |+ ||| #||+||+ | | |||++ +++ |||||+ | Sbjct 15 AKDKGAPAP#AASSKATKTGEPGMGSYRIMMFDQENFQGRMIE 56 |
| Canis lupus familiaris | Query 19 GLGGSYKVTVYELENFQGKRCE 40 | | +|+|+|+ ||||||| | Sbjct 26 GSAGPWKITIYDQENFQGKRME 47 |
| Canis familiaris | Query 7 APEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40 || # +|+ ||||+ |+| |||||+| | Sbjct 38 APAT#VPTTKAGDLPPGSYKLVVFEQENFQGRRVE 71 |
| Sus scrofa | Query 8 PEQ#AAASKSHGGLG----GSYKVTVYELENFQGKRCE 40 | |#| | | ||||+ |+| |||||+| | Sbjct 36 PAQ#APAQPMPAAKGDLPPGSYKLVVFEQENFQGRRVE 72 |
| Rana catesbeiana | Query 22 GSYKVTVYELENFQGKRCE 40 | +|+|||+ ||||||| | Sbjct 29 GPWKITVYDQENFQGKRME 47 |
| Rana temporaria | Query 22 GSYKVTVYELENFQGKRCE 40 | +|+|||+ ||||||| | Sbjct 12 GPWKITVYDQENFQGKRME 30 |
| synthetic construct | Query 22 GSYKVTVYELENFQGKRCE 40 |+|++ |+|||||||+| | Sbjct 57 GNYRLVVFELENFQGRRAE 75 |
| Contains: Beta crystallin A3, isoform A1, Delta4 form; Beta crystallin A3, isoform A1, Delta7 form; Beta crystallin A3, isoform A1, Delta8 form | Query 19 GLGGSYKVTVYELENFQGKRCE 40 | | +|+|+|+ ||||||| | Sbjct 26 GSVGPWKITIYDQENFQGKRME 47 |
| Oryctolagus cuniculus | Query 22 GSYKVTVYELENFQGKRCE 40 | +|+|+|+ ||||||| | Sbjct 29 GPWKITIYDQENFQGKRME 47 |
* References
[PubMed ID: 3879970] den Dunnen JT, Moormann RJ, Schoenmakers JG, Rat lens beta-crystallins are internally duplicated and homologous to gamma-crystallins. Biochim Biophys Acta. 1985 Apr 19;824(4):295-303.
[PubMed ID: 11773034] ... Lampi KJ, Shih M, Ueda Y, Shearer TR, David LL, Lens proteomics: analysis of rat crystallin sequences and two-dimensional electrophoresis map. Invest Ophthalmol Vis Sci. 2002 Jan;43(1):216-24.
[PubMed ID: 8405363] ... David LL, Shearer TR, Beta-crystallins insolubilized by calpain II in vitro contain cleavage sites similar to beta-crystallins insolubilized during cataract. FEBS Lett. 1993 Jun 21;324(3):265-70.