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XSB2080 : Amphiphysin [Homo sapiens]

[ CaMP Format ]

This entry is computationally expanded from SB0093

* Basic Information

OrganismHomo sapiens (human)
Protein Namesamphiphysin
Gene NamesAMPH; AMPH1
Gene LocusNot available
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
AAH34376 N/A 273 P49418 600418 471 N/A hsa:273

* Information From OMIM

Function: David et al. (1994) found that the N- and C-terminal domains of the amphiphysin protein are highly conserved between chicken and human. Autoantibodies from patients with the stiff-man syndrome show a dominant autoepitope located in the C-terminal region, which contains an SH3 domain.

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* Structure Information

1. Primary Information

Length: 695 aa

Average Mass: 76.230 kDa

Monoisotopic Mass: 76.183 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
BAR 1. 13233288.11.9e-83
Drf_FH1 1. 250397-40.24.3
--- cleavage 333 (inside Drf_FH1 250..397) ---
--- cleavage 392 (inside Drf_FH1 250..397) ---
--- cleavage 377 (inside Drf_FH1 250..397) ---
--- cleavage 454 ---
--- cleavage 531 ---
--- cleavage 609 ---
--- cleavage 478 ---
--- cleavage 593 ---
--- cleavage 527 ---
SH3_1 1. 62569424.10.00017

3. Sequence Information

Fasta Sequence: XSB2080.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1KY7 (X-ray; 215 A; P=322-331), 1UTC (X-ray; 230 A; P/Q=379-387)

* Cleavage Information

9 [sites]

Cleavage sites (±10aa)

[Site 1] TPAVGLDLGM454-DTRAEEPVEE

Met454 Asp

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Thr445Pro446Ala447Val448Gly449Leu450Asp451Leu452Gly453Met454
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asp455Thr456Arg457Ala458Glu459Glu460Pro461Val462Glu463Glu464

Sequence conservation (by blast)

[Site 2] SAQPEAEELE531-ATVPQEKVIP

Glu531 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser522Ala523Gln524Pro525Glu526Ala527Glu528Glu529Leu530Glu531
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala532Thr533Val534Pro535Gln536Glu537Lys538Val539Ile540Pro541

Sequence conservation (by blast)

[Site 3] APAMGAADQL609-ASAREASQEL

Leu609 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala600Pro601Ala602Met603Gly604Ala605Ala606Asp607Gln608Leu609
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala610Ser611Ala612Arg613Glu614Ala615Ser616Gln617Glu618Leu619

Sequence conservation (by blast)

[Site 4] PGADADAAVG478-TLVSAAEGAP

Gly478 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Pro469Gly470Ala471Asp472Ala473Asp474Ala475Ala476Val477Gly478
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Thr479Leu480Val481Ser482Ala483Ala484Glu485Gly486Ala487Pro488

Sequence conservation (by blast)

[Site 5] FEDNFVPEIS333-VTTPSQNEVP

Ser333 Val

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Phe324Glu325Asp326Asn327Phe328Val329Pro330Glu331Ile332Ser333
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Val334Thr335Thr336Pro337Ser338Gln339Asn340Glu341Val342Pro343

Sequence conservation (by blast)

[Site 6] LATEQKPIQD593-PQPTPSAPAM

Asp593 Pro

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Leu584Ala585Thr586Glu587Gln588Lys589Pro590Ile591Gln592Asp593
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Pro594Gln595Pro596Thr597Pro598Ser599Ala600Pro601Ala602Met603

Sequence conservation (by blast)

[Site 7] EGAESAQPEA527-EELEATVPQE

Ala527 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Glu518Gly519Ala520Glu521Ser522Ala523Gln524Pro525Glu526Ala527
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Glu528Glu529Leu530Glu531Ala532Thr533Val534Pro535Gln536Glu537

Sequence conservation (by blast)

[Site 8] DLWTTSTDLV392-QPASGGSFNG

Val392 Gln

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Asp383Leu384Trp385Thr386Thr387Ser388Thr389Asp390Leu391Val392
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gln393Pro394Ala395Ser396Gly397Gly398Ser399Phe400Asn401Gly402

Sequence conservation (by blast)

[Site 9] SAGVTHSPMS377-QTLPWDLWTT

Ser377 Gln

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser368Ala369Gly370Val371Thr372His373Ser374Pro375Met376Ser377
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gln378Thr379Leu380Pro381Trp382Asp383Leu384Trp385Thr386Thr387

Sequence conservation (by blast)

* References

[PubMed ID: 12477932] Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA, Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. Epub 2002 Dec 11.