XSB2131 : amphiphysin [Bos taurus]
[ CaMP Format ]
This entry is computationally expanded from SB0093
* Basic Information
| Organism | Bos taurus (cattle) |
| Protein Names | AMPH protein; amphiphysin |
| Gene Names | AMPH; amphiphysin |
| Gene Locus | 4; chromosome 4 |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 684 aa
Average Mass: 75.087 kDa
Monoisotopic Mass: 75.041 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| BAR 1. | 13 | 233 | 288.1 | 1.9e-83 |
| Drf_FH1 1. | 260 | 397 | -40.4 | 4.4 |
| --- cleavage 333 (inside Drf_FH1 260..397) --- |
| --- cleavage 392 (inside Drf_FH1 260..397) --- |
| --- cleavage 377 (inside Drf_FH1 260..397) --- |
| Calpain_inhib 1. | 451 | 580 | -24.7 | 2 |
| SH3_1 1. | 614 | 683 | 24.0 | 0.00018 |
3. Sequence Information
Fasta Sequence: XSB2131.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
3 [sites]
Cleavage sites (±10aa)
[Site 1] FEDNFVPEIS333-VTTPSQNEIP
Ser333 
Val
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Phe324 | Glu325 | Asp326 | Asn327 | Phe328 | Val329 | Pro330 | Glu331 | Ile332 | Ser333 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Val334 | Thr335 | Thr336 | Pro337 | Ser338 | Gln339 | Asn340 | Glu341 | Ile342 | Pro343 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
PPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEIPEVKKEETLLDLDFDPFKPDV
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
98.20 |
2 |
hypothetical protein LOC614722 |
| 2 |
Homo sapiens |
97.80 |
17 |
amphiphysin isoform 1 |
| 3 |
Pan troglodytes |
97.80 |
6 |
PREDICTED: amphiphysin |
| 4 |
Canis familiaris |
97.80 |
2 |
PREDICTED: similar to Amphiphysin |
| 5 |
synthetic construct |
97.80 |
1 |
amphiphysin |
| 6 |
Macaca mulatta |
96.30 |
4 |
PREDICTED: amphiphysin isoform 2 |
| 7 |
Rattus norvegicus |
91.70 |
3 |
amphiphysin 1 |
| 8 |
Monodelphis domestica |
91.70 |
2 |
PREDICTED: similar to cytokine receptor common gam |
| 9 |
Mus musculus |
90.10 |
4 |
amphiphysin |
| 10 |
Gallus gallus |
85.50 |
1 |
amphiphysin (Stiff-Man syndrome with breast cancer |
| 11 |
Xenopus tropicalis |
85.10 |
3 |
Unknown (protein for MGC:122074) |
| 12 |
Xenopus laevis |
85.10 |
1 |
hypothetical protein LOC432150 |
| 13 |
N/A |
59.30 |
1 |
A |
| 14 |
Danio rerio |
54.70 |
1 |
amphiphysin |
| 15 |
Tetraodon nigroviridis |
48.10 |
1 |
unnamed protein product |
| 16 |
Lampetra fluviatilis |
47.80 |
1 |
amphiphysin |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||||||||||||||#|||||||||||||||||
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
|
| Homo sapiens |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||||||||||||||#||||||||+||||||||
Sbjct 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEVPEVKKEET 350
|
| Pan troglodytes |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||||||||||||||#||||||||+||||||||
Sbjct 407 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEVPEVKKEET 453
|
| Canis familiaris |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||||||||||||||#||||||||+||||||||
Sbjct 323 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEVPEVKKEET 369
|
| synthetic construct |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||||||||||||||#||||||||+||||||||
Sbjct 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEVPEVKKEET 350
|
| Macaca mulatta |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||+|||||||||||||||||#||||||||+||||||||
Sbjct 304 PPLPKVTPTKELRQENIISFFEDNFVPEIS#VTTPSQNEVPEVKKEET 350
|
| Rattus norvegicus |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||+||||||||||+#||||||||+ |||||||
Sbjct 304 PPLPKVTPTKELQQENIINFFEDNFVPEIN#VTTPSQNEVLEVKKEET 350
|
| Monodelphis domestica |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||||||||| ||||||||||#||||||||+|| ||||+
Sbjct 304 PPLPKVTPTKELQQENIISLFEDNFVPEIS#VTTPSQNEVPEQKKEES 350
|
| Mus musculus |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
||||||||||||+|||||+||||||||||+#||||||||+ |||||||
Sbjct 304 PPLPKVTPTKELKQENIINFFEDNFVPEIN#VTTPSQNEVLEVKKEET 350
|
| Gallus gallus |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
|||||+||||||||||||+ |+|||||||+#||||||||||| || |+
Sbjct 304 PPLPKLTPTKELQQENIINLFDDNFVPEIN#VTTPSQNEIPETKKVES 350
|
| Xenopus tropicalis |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
|||||+||+||||||||||||||||||||+#|||||||| | ||||+
Sbjct 305 PPLPKLTPSKELQQENIISFFEDNFVPEIN#VTTPSQNEAVEEKKEES 351
|
| Xenopus laevis |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEVKKEET 350
|||||+||+||||||||||||||||||||+#|||||||| | ||||+
Sbjct 309 PPLPKLTPSKELQQENIISFFEDNFVPEIN#VTTPSQNEAVEEKKEES 355
|
| N/A |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQ 339
|| || ||+||++|| |+| ||| ||||||#||||||
Sbjct 42 PPPPKHTPSKEVKQEQILSLFEDTFVPEIS#VTTPSQ 77
|
| Danio rerio |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIP 343
|| ||||||+||||| || |+ | ||||#||+| || |
Sbjct 304 PPPPKVTPTRELQQEQIIDLFDGGF-PEIS#VTSPQPNERP 342
|
| Tetraodon nigroviridis |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS#VTTPSQNEIPEV 345
|| |||||+|+++ |||++ |+ |+||#||+|++ + | |
Sbjct 212 PPPPKVTPSKDMKAENIVNLFDAAAAPDIS#VTSPTEFDRPAV 253
|
| Lampetra fluviatilis |
Query 304 PPLPKVTPTKELQQENIISFFEDNFVPEIS# 333
|| ||+||+||+||| | | |+|||||+ |#
Sbjct 303 PPPPKLTPSKEVQQEAIFSLFDDNFVPDFS# 332
|
[Site 2] DLWTTSTDLV392-QPASGGSFNG
Val392 Gln
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Asp383 | Leu384 | Trp385 | Thr386 | Thr387 | Ser388 | Thr389 | Asp390 | Leu391 | Val392 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln393 | Pro394 | Ala395 | Ser396 | Gly397 | Gly398 | Ser399 | Phe400 | Asn401 | Gly402 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
VTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQDTSLFTMQADQSMFNMA
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
127.00 |
2 |
hypothetical protein LOC614722 |
| 2 |
Homo sapiens |
112.00 |
10 |
amphiphysin isoform 1 |
| 3 |
synthetic construct |
112.00 |
1 |
amphiphysin |
| 4 |
Macaca mulatta |
108.00 |
3 |
PREDICTED: amphiphysin isoform 2 |
| 5 |
Rattus norvegicus |
104.00 |
1 |
amphiphysin 1 |
| 6 |
Mus musculus |
102.00 |
2 |
amphiphysin |
| 7 |
Monodelphis domestica |
89.70 |
1 |
PREDICTED: similar to cytokine receptor common gam |
| 8 |
Gallus gallus |
81.30 |
1 |
amphiphysin (Stiff-Man syndrome with breast cancer |
| 9 |
Xenopus tropicalis |
67.80 |
1 |
Unknown (protein for MGC:122074) |
| 10 |
Xenopus laevis |
58.90 |
1 |
hypothetical protein LOC432150 |
| 11 |
Pan troglodytes |
47.00 |
1 |
PREDICTED: amphiphysin |
| 12 |
Canis familiaris |
44.70 |
1 |
PREDICTED: similar to Amphiphysin |
| 13 |
Danio rerio |
43.10 |
1 |
amphiphysin |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFTQDTSLFTMQADQSMFNMA 422
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFTQDTSLFTMQADQSMFNMA 422
|
| Homo sapiens |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQSM 418
||||||||||||||||||||||||||||||#|||||||||||| ||||||||| ||||
Sbjct 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFTQPQDTSLFTMQTDQSM 420
|
| synthetic construct |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQSM 418
||||||||||||||||||||||||||||||#|||||||||||| ||||||||| ||||
Sbjct 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFTQPQDTSLFTMQTDQSM 420
|
| Macaca mulatta |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQSM 418
|||||||||||||||||||||||||| |||#|||||||||||| |||||| || ||||
Sbjct 363 VTPAGSAGVTHSPMSQTLPWDLWTTSADLV#QPASGGSFNGFTQPQDTSLFAMQTDQSM 420
|
| Rattus norvegicus |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQSM 418
||||||| |||||||||||||||||||||#||||||||| || ||||||||| ||+|
Sbjct 363 VTPAGSAAATHSPMSQTLPWDLWTTSTDLV#QPASGGSFNDFTQPQDTSLFTMQTDQNM 420
|
| Mus musculus |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQSM 418
| ||||| |||||||||||||||||||||#||||||||| || ||||||||| ||+|
Sbjct 363 VAPAGSAAATHSPMSQTLPWDLWTTSTDLV#QPASGGSFNDFTQAQDTSLFTMQTDQNM 420
|
| Monodelphis domestica |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQS 417
||| ||||+ |||||||||||||||++||#|||| |+||||| |||||| | | |+
Sbjct 363 VTPVGSAGIAPSPMSQTLPWDLWTTSSELV#QPASSGTFNGFTQPQDTSLFDMPASQT 419
|
| Gallus gallus |
Query 368 SAGVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFTQDTSLFTMQADQSM 418
| ||||||||||||||||||+++||#|||| +|||| |||+ | +|+++++
Sbjct 365 STGVTHSPMSQTLPWDLWTTTSELV#QPASSTAFNGFAQDTTAFAVQSNENV 415
|
| Xenopus tropicalis |
Query 372 THSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFTQ---DTSLFTMQADQS 417
+|||+|||||||||||+ ++|#|||| |+|||||| |||+ +|+
Sbjct 372 SHSPLSQTLPWDLWTTNNEVV#QPASTGAFNGFTQPDASAPLFTLPLEQN 420
|
| Xenopus laevis |
Query 366 AGSAGV--THSPMSQTLPWDLWT-------TSTDLV#QPASGGSFNGFTQ---DTSLFTMQ 413
| + |+ +|||+|||||||||| |+ ++|#|||| | |||||| |||+
Sbjct 368 AATVGIVQSHSPISQTLPWDLWTCFCPSTQTNNEVV#QPASTGVFNGFTQPDASAPLFTLP 427
Query 414 ADQS 417
++++
Sbjct 428 SEKN 431
|
| Pan troglodytes |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QP 394
||||||||||||||||||||||||||||||#||
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QP 394
Query 378 QTLPWDLWTTSTDLV#QPASGGSFNGFT--QDTSLFTMQADQSM 418
| + ||+ + #| |||||||||| ||||||||| |||+
Sbjct 656 QKIEDSLWS-GVEAC#QKASGGSFNGFTQPQDTSLFTMQTDQSV 697
|
| Canis familiaris |
Query 363 VTPAGSAGVTHSPMSQTLPWDLWTTSTDLV#QP 394
| | || |||||||||||||||||||||||#||
Sbjct 382 VAPVGSVGVTHSPMSQTLPWDLWTTSTDLV#QP 413
Query 378 QTLPWDLWTTSTDLV#QPASGGSFNGFTQ--DTSLFTMQADQSM 418
| + +| + #+ ||||+|||||| |||||||| ||||
Sbjct 572 QKIEDSVWA-GVETC#EKASGGTFNGFTQPQDTSLFTMQTDQSM 613
|
| Danio rerio |
Query 370 GVTHSPMSQTLPWDLWTTSTDLV#QPASGGSFNGFT 404
| | ||+|||||||||| | #||| + |||
Sbjct 363 GQTQSPVSQTLPWDLWT--GDAA#QPAQPATDAGFT 395
|
[Site 3] SAGVTHSPMS377-QTLPWDLWTT
Ser377 Gln
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Ser368 | Ala369 | Gly370 | Val371 | Thr372 | His373 | Ser374 | Pro375 | Met376 | Ser377 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Gln378 | Thr379 | Leu380 | Pro381 | Trp382 | Asp383 | Leu384 | Trp385 | Thr386 | Thr387 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
EETLLDLDFDPFKPDVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQDT
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
99.80 |
2 |
hypothetical protein LOC614722 |
| 2 |
Homo sapiens |
95.50 |
7 |
amphiphysin isoform 1 |
| 3 |
synthetic construct |
95.50 |
1 |
amphiphysin |
| 4 |
Macaca mulatta |
93.60 |
3 |
PREDICTED: amphiphysin isoform 2 |
| 5 |
Rattus norvegicus |
89.00 |
1 |
amphiphysin 1 |
| 6 |
Mus musculus |
87.00 |
2 |
amphiphysin |
| 7 |
Monodelphis domestica |
80.10 |
1 |
PREDICTED: similar to cytokine receptor common gam |
| 8 |
Gallus gallus |
74.30 |
1 |
amphiphysin (Stiff-Man syndrome with breast cancer |
| 9 |
Pan troglodytes |
73.20 |
1 |
PREDICTED: amphiphysin |
| 10 |
Canis familiaris |
68.20 |
1 |
PREDICTED: similar to Amphiphysin |
| 11 |
Xenopus tropicalis |
64.70 |
1 |
Unknown (protein for MGC:122074) |
| 12 |
Xenopus laevis |
56.60 |
1 |
hypothetical protein LOC432150 |
| 13 |
Danio rerio |
43.10 |
1 |
amphiphysin |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQDT 407
||| |||||||||||||||#||||||||||||||||||||||||||||||
Sbjct 348 EETLLDLDFDPFKPDVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQDT 407
|
| Homo sapiens |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
||| |||||||||||||||#||||||||||||||||||||||||||||
Sbjct 348 EETLLDLDFDPFKPEVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
|
| synthetic construct |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
||| |||||||||||||||#||||||||||||||||||||||||||||
Sbjct 348 EETLLDLDFDPFKPEVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
|
| Macaca mulatta |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
||| |||||||||||||||#||||||||||| ||||||||||||||||
Sbjct 348 EETLLDLDFDPFKPEVTPAGSAGVTHSPMS#QTLPWDLWTTSADLVQPASGGSFNGFTQ 405
|
| Rattus norvegicus |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
||| ||||||| ||||||#|||||||||||||||||||||||| |||
Sbjct 348 EETLLDLDFDPFKPDVTPAGSAAATHSPMS#QTLPWDLWTTSTDLVQPASGGSFNDFTQ 405
|
| Mus musculus |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
||| | ||||| ||||||#|||||||||||||||||||||||| |||
Sbjct 348 EETLLDLDFDPFKPDVAPAGSAAATHSPMS#QTLPWDLWTTSTDLVQPASGGSFNDFTQ 405
|
| Monodelphis domestica |
Query 363 VTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
||| ||||+ ||||#|||||||||||++|||||| |+||||||
Sbjct 363 VTPVGSAGIAPSPMS#QTLPWDLWTTSSELVQPASSGTFNGFTQ 405
|
| Gallus gallus |
Query 368 SAGVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQDT 407
| ||||||||#||||||||||+++|||||| +|||| |||
Sbjct 365 STGVTHSPMS#QTLPWDLWTTTSELVQPASSTAFNGFAQDT 404
|
| Pan troglodytes |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQP 394
||| |||||||||||||||#|||||||||||||||||
Sbjct 451 EETLLDLDFDPFKPEVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQP 497
|
| Canis familiaris |
Query 348 EETXXXXXXXXXXXXVTPAGSAGVTHSPMS#QTLPWDLWTTSTDLVQP 394
||| | | || ||||||||#|||||||||||||||||
Sbjct 367 EETLLDLDFDPFKPEVAPVGSVGVTHSPMS#QTLPWDLWTTSTDLVQP 413
|
| Xenopus tropicalis |
Query 372 THSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFTQ 405
+|||+|#||||||||||+ ++||||| |+||||||
Sbjct 372 SHSPLS#QTLPWDLWTTNNEVVQPASTGAFNGFTQ 405
|
| Xenopus laevis |
Query 366 AGSAGV--THSPMS#QTLPWDLWT-------TSTDLVQPASGGSFNGFTQ 405
| + |+ +|||+|#||||||||| |+ ++||||| | ||||||
Sbjct 368 AATVGIVQSHSPIS#QTLPWDLWTCFCPSTQTNNEVVQPASTGVFNGFTQ 416
|
| Danio rerio |
Query 370 GVTHSPMS#QTLPWDLWTTSTDLVQPASGGSFNGFT 404
| | ||+|#||||||||| | ||| + |||
Sbjct 363 GQTQSPVS#QTLPWDLWT--GDAAQPAQPATDAGFT 395
|
