XSB2165 : cortactin, isoform CRA_c [Rattus norvegicus]
[ CaMP Format ]
This entry is computationally expanded from SB0022
* Basic Information
| Organism | Rattus norvegicus (Norway rat) |
| Protein Names | cortactin; isoform CRA_c |
| Gene Names | Cttn |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 546 aa
Average Mass: 61.077 kDa
Monoisotopic Mass: 61.040 kDa
2. Domain Information
Annotated Domains: Not Available.
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| HS1_rep 1. | 83 | 119 | 68.2 | 3.1e-17 |
| HS1_rep 2. | 120 | 156 | 72.7 | 1.3e-18 |
| HS1_rep 3. | 157 | 193 | 74.7 | 3.4e-19 |
| HS1_rep 4. | 194 | 230 | 69.9 | 9.2e-18 |
| HS1_rep 5. | 231 | 267 | 68.5 | 2.5e-17 |
| HS1_rep 6. | 268 | 304 | 71.5 | 3.1e-18 |
| HS1_rep 7. | 305 | 341 | 26.6 | 0.0001 |
| --- cleavage 336 (inside HS1_rep 305..341) --- | ||||
| --- cleavage 346 --- | ||||
| --- cleavage 358 --- | ||||
| SH3_1 1. | 491 | 545 | 89.7 | 1e-23 |
3. Sequence Information
Fasta Sequence: XSB2165.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
3 [sites]
Cleavage sites (±10aa)
[Site 1] TVPIEAVTSK346-TSNIRANFEN
Lys346 
Thr
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Thr337 | Val338 | Pro339 | Ile340 | Glu341 | Ala342 | Val343 | Thr344 | Ser345 | Lys346 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr347 | Ser348 | Asn349 | Ile350 | Arg351 | Ala352 | Asn353 | Phe354 | Glu355 | Asn356 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 117.00 | 5 | cortactin |
| 2 | Rattus norvegicus | 117.00 | 4 | cortactin isoform B |
| 3 | N/A | 117.00 | 2 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 117.00 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 110.00 | 19 | SRC8_HUMAN Src substrate cortactin (Amplaxin) (Onc |
| 6 | synthetic construct | 110.00 | 3 | cortactin |
| 7 | Macaca mulatta | 110.00 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Macaca fascicularis | 110.00 | 2 | unnamed protein product |
| 9 | Pongo pygmaeus | 108.00 | 1 | hypothetical protein |
| 10 | Bos taurus | 105.00 | 3 | cortactin |
| 11 | Canis familiaris | 104.00 | 6 | PREDICTED: similar to cortactin isoform a isoform |
| 12 | Gallus gallus | 91.30 | 2 | cortactin |
| 13 | Xenopus laevis | 85.90 | 4 | MGC80961 protein |
| 14 | Danio rerio | 72.00 | 12 | cortactin |
| 15 | Tetraodon nigroviridis | 71.60 | 4 | unnamed protein product |
| 16 | Xenopus tropicalis | 63.50 | 1 | cortactin |
| 17 | Aedes aegypti | 49.70 | 1 | cortactin |
| 18 | Pan troglodytes | 48.10 | 9 | PREDICTED: hypothetical protein isoform 1 |
| 19 | Strongylocentrotus purpuratus | 47.80 | 1 | SRC8 protein |
| 20 | Drosophila melanogaster | 39.30 | 2 | Cortactin |
| 21 | Drosophila pseudoobscura | 38.10 | 1 | GA17577-PA |
| 22 | Monodelphis domestica | 35.80 | 2 | PREDICTED: similar to KIAA1561 protein |
| 23 | Parvibaculum lavamentivorans DS-1 | 35.80 | 1 | translation initiation factor IF-2 |
| 24 | Anopheles gambiae str. PEST | 33.50 | 1 | ENSANGP00000029554 |
| 25 | Neurospora crassa | 32.70 | 1 | related to putative cytoplasmic structural protein |
| 26 | Neurospora crassa OR74A | 32.70 | 1 | hypothetical protein |
| 27 | Arabidopsis thaliana | 32.30 | 2 | unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |
| Rattus norvegicus | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |
| N/A | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |
| Homo sapiens | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| synthetic construct | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| Macaca mulatta | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| Macaca fascicularis | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 1 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 60 |
| Pongo pygmaeus | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| || ||||||||+||| ||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVASK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| Bos taurus | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| | |+||||||+||| ||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVAAVSSSYQKTVPVEAVNSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| Canis familiaris | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||||||+| || |||||||+||| |+#||||||||||||||+|||||||||||+||| Sbjct 317 MDKNASTFEDVAQVAPAYQKTVPVEAVNSR#TSNIRANFENLAKEKEQEDRRKAEAEKAQR 376 |
| Gallus gallus | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||||+|||++ + | |||| |+| | +|#||+|||| ||||||+||||||||||||||| Sbjct 326 MDKNAATFEDIEKPTSTYQKTKPVERVANK#TSSIRANLENLAKEKEQEDRRKAEAERAQR 385 |
| Xenopus laevis | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ||| |++||+| +| |+|||| |+| ||# |+||||||||||++|||||+||||||||| Sbjct 315 MDKAAASFEDVEKVSSSYQKTRPVEVEGSK#ASSIRANFENLAKDKEQEDRKKAEAERAQR 374 |
| Danio rerio | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||+| ||||| + +||||| |+|| +| # |+|+| |||+||++|+|++++ | ||++| Sbjct 316 MDKSAGTFEEVQKPSAAYQKTRPVEAASSS#ASSIKARFENIAKQKEEEEQQRLEEERSRR 375 |
| Tetraodon nigroviridis | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ||| | ||||| + +|||| |+|| | # +|+| |||+||++| ||||+|| || +| Sbjct 325 MDKAAGTFEEVEKPTPSYQKTKPVEAAGSN#AGSIKARFENMAKQKEDEDRRRAEEERLRR 384 |
| Xenopus tropicalis | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 +|| || | |+ |||+|| |+||+|| # |+|+ |||+|+ |+|+++||| || +| Sbjct 278 VDKCASGFGEIETPSSAYEKTQPLEAMTSG#AHNLRSRFENMARTAEEENKQKAEEERVRR 337 |
| Aedes aegypti | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 |||+| |+| ++ + | || | + ++|# ||||| ||| | |+| |+||| ++ | Sbjct 241 MDKSALGFQEQDKIGTNYTKTKP-DIGSAK#PSNIRARFENFAMAAEEETRKKAEEQKRLR 299 |
| Pan troglodytes | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKE---REQEDRRKAEAER 373 +||+| | |+ +||+|| |||| +| # ++| ||++|+| ||+|++ + | | Sbjct 246 VDKSAVGFNEMEAPTTAYKKTTPIEAASSG#ARGLKAKFESMAEEKRKREEEEKAQQVARR 305 Query 374 AQ 375 | Sbjct 306 QQ 307 |
| Strongylocentrotus purpuratus | Query 318 DKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 | | | ++ | |+|+|| | | # |+| || +|+ |+| ||||| || +| Sbjct 281 DATAGGFGDMQGVSSSYKKTRPQPPAKSG#AGNMRNKFEQMAQAGEEESRRKAEEERGRR 339 |
| Drosophila melanogaster | Query 345 SK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 +|# ||+|| |||||| |+| |++|| ++ | Sbjct 238 AK#PSNLRAKFENLAKNSEEESRKRAEEQKRLR 269 |
| Drosophila pseudoobscura | Query 345 SK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 +|# ||+|| |||||| |+| |+++| ++ | Sbjct 236 AK#PSNLRAKFENLAKNSEEESRKRSEEQKRLR 267 |
| Monodelphis domestica | Query 317 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERA 374 +++|| ||+ + || ||+ | + # +++ + | | +| + + || ||+|| Sbjct 1146 LEENAKQNSEVLAAQALLQKHVPLGQVEAL#KNSLSSTIEALREELKDKQRRHEEAQRA 1203 |
| Parvibaculum lavamentivorans DS-1 | Query 327 VVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAE 372 | + || + | + +| +# | || + |+||| ||||+|| | Sbjct 118 VEKKPSRSRSGVVLRTLTEE#EKNARAQALDSAREREGEDRRRAEEE 163 |
| Anopheles gambiae str. PEST | Query 344 TSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 ++|# ||+|| ||| | |+| |++|+ ++ | Sbjct 237 SAK#PSNLRAKFENFAATAEEEARKRADEQKRLR 269 |
| Neurospora crassa | Query 325 EEVVQVPSAYQKTVPIEAV----------TSK#TSNIRANFENLAKER-EQEDRRKAEAER 373 || + || +|+|| +| | +# + +| | +|+|+ |+ +| ||| |+ Sbjct 1529 EETLYVPPQHQETVDTKAQEDQLKAQKVETER#LEHEKAEQERVAREKAERAEREKAEREK 1588 Query 374 AQR 376 |+| Sbjct 1589 AER 1591 |
| Neurospora crassa OR74A | Query 325 EEVVQVPSAYQKTVPIEAV----------TSK#TSNIRANFENLAKER-EQEDRRKAEAER 373 || + || +|+|| +| | +# + +| | +|+|+ |+ +| ||| |+ Sbjct 1452 EETLYVPPQHQETVDTKAQEDQLKAQKVETER#LEHEKAEQERVAREKAERAEREKAEREK 1511 Query 374 AQR 376 |+| Sbjct 1512 AER 1514 |
| Arabidopsis thaliana | Query 342 AVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 376 | |# | +|+ + + ||+|+|||++ || + +| Sbjct 736 ARADK#LRNFKADLQKMKKEKEEEDRKRIEALKIER 770 |
[Site 2] VVQVPSAYQK336-TVPIEAVTSK
Lys336 Thr
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val327 | Val328 | Gln329 | Val330 | Pro331 | Ser332 | Ala333 | Tyr334 | Gln335 | Lys336 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr337 | Val338 | Pro339 | Ile340 | Glu341 | Ala342 | Val343 | Thr344 | Ser345 | Lys346 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| GGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 120.00 | 5 | cortactin |
| 2 | Rattus norvegicus | 120.00 | 4 | cortactin isoform B |
| 3 | N/A | 120.00 | 2 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 120.00 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 113.00 | 10 | cortactin isoform a |
| 6 | synthetic construct | 113.00 | 3 | cortactin |
| 7 | Macaca mulatta | 113.00 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Pongo pygmaeus | 111.00 | 1 | hypothetical protein |
| 9 | Canis familiaris | 108.00 | 5 | PREDICTED: similar to cortactin isoform a isoform |
| 10 | Bos taurus | 108.00 | 2 | cortactin |
| 11 | Gallus gallus | 94.40 | 2 | cortactin |
| 12 | Macaca fascicularis | 92.40 | 2 | unnamed protein product |
| 13 | Xenopus laevis | 90.10 | 3 | MGC80961 protein |
| 14 | Danio rerio | 84.30 | 10 | cortactin |
| 15 | Tetraodon nigroviridis | 79.70 | 3 | unnamed protein product |
| 16 | Strongylocentrotus purpuratus | 48.10 | 1 | SRC8 protein |
| 17 | Drosophila melanogaster | 47.00 | 2 | Cortactin |
| 18 | Drosophila pseudoobscura | 47.00 | 1 | GA17577-PA |
| 19 | Pan troglodytes | 43.50 | 5 | PREDICTED: cortactin isoform 3 |
| 20 | Tribolium castaneum | 42.40 | 1 | PREDICTED: similar to Xaa-Pro dipeptidase (X-Pro d |
| 21 | Monodelphis domestica | 39.30 | 1 | PREDICTED: hypothetical protein |
| 22 | Suberites domuncula | 35.40 | 1 | cortactin |
| 23 | Anopheles gambiae str. PEST | 33.50 | 1 | ENSANGP00000029554 |
| 24 | Xenopus tropicalis | 33.10 | 1 | cortactin |
| 25 | Aedes aegypti | 32.30 | 1 | cortactin |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |
| Rattus norvegicus | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |
| N/A | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |
| Homo sapiens | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 |
| synthetic construct | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 329 |
| Macaca mulatta | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 |
| Pongo pygmaeus | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||||||+| || |||||#|||+||| ||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVASKTSNIRANFENLAKEKEQEDR 329 |
| Canis familiaris | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||||||+| || ||||#|||+||| |+||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVAQVAPAYQK#TVPVEAVNSRTSNIRANFENLAKEKEQEDR 366 |
| Bos taurus | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||||||+| | |+|||#|||+||| ||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVAAVSSSYQK#TVPVEAVNSKTSNIRANFENLAKEKEQEDR 366 |
| Gallus gallus | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||||+|||++ + | |||#| |+| | +|||+|||| ||||||+||||| Sbjct 316 GGKYGVQKDRMDKNAATFEDIEKPTSTYQK#TKPVERVANKTSSIRANLENLAKEKEQEDR 375 |
| Macaca fascicularis | Query 317 MDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 1 MDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 50 |
| Xenopus laevis | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 ||||||||||||| |++||+| +| |+|||#| |+| || |+||||||||||++||||| Sbjct 305 GGKYGVQKDRMDKAAASFEDVEKVSSSYQK#TRPVEVEGSKASSIRANFENLAKDKEQEDR 364 |
| Danio rerio | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||||||||||||+| ||||| + +||||#| |+|| +| |+|+| |||+||++|+|++ Sbjct 306 GGKYGVQKDRMDKSAGTFEEVQKPSAAYQK#TRPVEAASSSASSIKARFENIAKQKEEEEQ 365 |
| Tetraodon nigroviridis | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||+||||||||| | ||||| + +|||#| |+|| | +|+| |||+||++| ||| Sbjct 315 GGKFGVQKDRMDKAAGTFEEVEKPTPSYQK#TKPVEAAGSNAGSIKARFENMAKQKEDEDR 374 |
| Strongylocentrotus purpuratus | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||+|| |+ | | | ++ | |+|+|#| | | |+| || +|+ |+| | Sbjct 270 GGKFGVDKNAQDATAGGFGDMQGVSSSYKK#TRPQPPAKSGAGNMRNKFEQMAQAGEEESR 329 |
| Drosophila melanogaster | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVT----SKTSNIRANFENLAKERE 362 |||+|||+|| ||+| ++ + | + # | + +| ||+|| |||||| | Sbjct 197 GGKFGVQEDRKDKSAVGWDHK-EAPQKHAS#QVDHKVKPVIEGAKPSNLRAKFENLAKNSE 255 Query 363 QEDR 366 +| | Sbjct 256 EESR 259 |
| Drosophila pseudoobscura | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVT----SKTSNIRANFENLAKERE 362 |||+|||+|| ||+| ++ + | + # | + +| ||+|| |||||| | Sbjct 195 GGKFGVQEDRKDKSAVGWDHK-EAPQKHAS#QVDHKVKPVIEGAKPSNLRAKFENLAKNSE 253 Query 363 QEDR 366 +| | Sbjct 254 EESR 257 |
| Pan troglodytes | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKT 347 |||||||||||||++ + + + | ||# |+ |+ + | Sbjct 307 GGKYGVQKDRMDKSSPSLGQTIVESPAMQK#GFPVAAIVTTT 347 |
| Tribolium castaneum | Query 307 GGKYGVQKDRMDKNASTF-EEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENL 357 |||+|+| ||+||+|+ | +| +| + | |# | + +| ++|| |||+ Sbjct 736 GGKFGLQTDRVDKSAANFNDEPAKVGTNYTK#VKP-DIGDAKPKDLRAKFENM 786 |
| Monodelphis domestica | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTS---KTSNIRANFENLAKEREQ 363 ||||||||||||| | | | |#+ + | | |++ | | + | Sbjct 270 GGKYGVQKDRMDKRGGTAEPQAQSGRPQTK#SSLLTLATGVPCSISGIKSAFSNHQTPQAQ 329 Query 364 ED 365 | Sbjct 330 SD 331 |
| Suberites domuncula | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLA 358 ||||||++ | +| ++++ | | ++ # + +| +||+ |||+| Sbjct 271 GGKYGVEEGNQDSSAGGYDDMQAVKSDHRT#ERGVS--KGQTGSIRSKFENMA 320 |
| Anopheles gambiae str. PEST | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAV------TSKTSNIRANFENLAKE 360 |||+||| || ||+| ++ |+ | ++ # + + | ++| ||+|| ||| | Sbjct 195 GGKFGVQTDRKDKSAFGWDH-VEKPQMHES#QLDHKIVIKPDIGSAKPSNLRAKFENFAAT 253 Query 361 REQEDR 366 |+| | Sbjct 254 AEEEAR 259 Query 307 GGKYGVQKDRMDKNASTFEEVVQV 330 |||+||+||||||+| | + +| Sbjct 84 GGKFGVEKDRMDKSAVGHEHIEKV 107 Query 307 GGKYGVQKDRMDKNASTFEEVVQV 330 |||+||||||+||+| ++ | +| Sbjct 121 GGKFGVQKDRVDKSAHGWDHVEKV 144 |
| Xenopus tropicalis | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 ||++||| ||+|| || | |+ |||+|#| |+||+|| |+|+ |||+|+ |+|++ Sbjct 268 GGRFGVQTDRVDKCASGFGEIETPSSAYEK#TQPLEAMTSGAHNLRSRFENMARTAEEENK 327 Query 307 GGKYGVQKDRMDKNASTFEEVVQV 330 |||||||||| ||+| |+ +| Sbjct 120 GGKYGVQKDRTDKSAVGFDYKAKV 143 Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQE 364 |||||||||| ||+| ++ | ++# | ++ | | | + |+|+ + Sbjct 194 GGKYGVQKDRQDKSAHSW-------SHKEE#LQPHQSQTDYTQGFGGKF-GVQKDRQDK 243 |
| Aedes aegypti | Query 307 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 366 |||+||| |||||+| |+| ++ + | |#| | + ++| ||||| ||| | |+| | Sbjct 231 GGKFGVQSDRMDKSALGFQEQDKIGTNYTK#TKP-DIGSAKPSNIRARFENFAMAAEEETR 289 Query 307 GGKYGVQKDRMDKNASTFEEVVQV 330 |||+||| ||+|| |+|+| +| Sbjct 120 GGKFGVQSDRVDKCAATWEHKEKV 143 |
[Site 3] NIRANFENLA358-KEREQEDRRK
Ala358 Lys
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Asn349 | Ile350 | Arg351 | Ala352 | Asn353 | Phe354 | Glu355 | Asn356 | Leu357 | Ala358 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Lys359 | Glu360 | Arg361 | Glu362 | Gln363 | Glu364 | Asp365 | Arg366 | Arg367 | Lys368 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| QVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEAR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 63.20 | 5 | cortactin |
| 2 | Rattus norvegicus | 63.20 | 4 | cortactin isoform B |
| 3 | N/A | 63.20 | 1 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 63.20 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 59.70 | 10 | cortactin isoform a variant |
| 6 | synthetic construct | 59.70 | 3 | cortactin |
| 7 | Macaca mulatta | 59.70 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Macaca fascicularis | 59.70 | 2 | unnamed protein product |
| 9 | Pongo pygmaeus | 57.80 | 1 | hypothetical protein |
| 10 | Canis familiaris | 54.70 | 5 | PREDICTED: similar to cortactin isoform a isoform |
| 11 | Bos taurus | 54.70 | 2 | cortactin |
| 12 | Xenopus laevis | 43.90 | 3 | MGC80961 protein |
| 13 | Gallus gallus | 43.50 | 2 | cortactin |
| 14 | Danio rerio | 40.00 | 1 | cortactin |
| 15 | Xenopus tropicalis | 38.90 | 1 | cortactin |
| 16 | Tetraodon nigroviridis | 35.80 | 2 | unnamed protein product |
| 17 | Pan troglodytes | 32.30 | 2 | PREDICTED: hypothetical protein isoform 1 |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 ||||||||||||||||||||||||||||||#| Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 |
| Rattus norvegicus | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 ||||||||||||||||||||||||||||||#| Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 |
| N/A | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 ||||||||||||||||||||||||||||||#| Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 ||||||||||||||||||||||||||||||#| Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 |
| Homo sapiens | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 || ||||||||+||||||||||||||||||#| Sbjct 329 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 359 |
| synthetic construct | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 || ||||||||+||||||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 322 |
| Macaca mulatta | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 || ||||||||+||||||||||||||||||#| Sbjct 329 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 359 |
| Macaca fascicularis | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 || ||||||||+||||||||||||||||||#| Sbjct 13 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 43 |
| Pongo pygmaeus | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 || ||||||||+||| ||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVASKTSNIRANFENLA#K 322 |
| Canis familiaris | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 || |||||||+||| |+||||||||||||#| Sbjct 329 QVAPAYQKTVPVEAVNSRTSNIRANFENLA#K 359 |
| Bos taurus | Query 330 VPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 | |+||||||+||| ||||||||||||||#| Sbjct 330 VSSSYQKTVPVEAVNSKTSNIRANFENLA#K 359 |
| Xenopus laevis | Query 329 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 359 +| |+|||| |+| || |+|||||||||#| Sbjct 327 KVSSSYQKTRPVEVEGSKASSIRANFENLA#K 357 |
| Gallus gallus | Query 332 SAYQKTVPIEAVTSKTSNIRANFENLA#K 359 | |||| |+| | +|||+|||| ||||#| Sbjct 341 STYQKTKPVERVANKTSSIRANLENLA#K 368 |
| Danio rerio | Query 329 QVPSA-YQKTVPIEAVTSKTSNIRANFENLA#K 359 | ||| |||| |+|| +| |+|+| |||+|#| Sbjct 327 QKPSAAYQKTRPVEAASSSASSIKARFENIA#K 358 |
| Xenopus tropicalis | Query 332 SAYQKTVPIEAVTSKTSNIRANFENLA#K 359 |||+|| |+||+|| |+|+ |||+|#+ Sbjct 293 SAYEKTQPLEAMTSGAHNLRSRFENMA#R 320 |
| Tetraodon nigroviridis | Query 333 AYQKTVPIEAVTSKTSNIRANFENLA#K 359 +|||| |+|| | +|+| |||+|#| Sbjct 341 SYQKTKPVEAAGSNAGSIKARFENMA#K 367 |
| Pan troglodytes | Query 332 SAYQKTVPIEAVTSKTSNIRANFENLA#K 359 +||+|| |||| +| ++| ||++|#+ Sbjct 261 TAYKKTTPIEAASSGARGLKAKFESMA#E 288 |
* References
[PubMed ID: 15632090] ,