XSB2166 : cortactin, isoform CRA_d [Rattus norvegicus]
[ CaMP Format ]
This entry is computationally expanded from SB0022
* Basic Information
| Organism | Rattus norvegicus (Norway rat) |
| Protein Names | cortactin; isoform CRA_d |
| Gene Names | Cttn |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 381 aa
Average Mass: 42.737 kDa
Monoisotopic Mass: 42.710 kDa
2. Domain Information
Annotated Domains: Not Available.
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| HS1_rep 1. | 1 | 28 | 35.4 | 2.2e-07 |
| HS1_rep 2. | 29 | 65 | 74.7 | 3.4e-19 |
| HS1_rep 3. | 66 | 102 | 69.9 | 9.2e-18 |
| HS1_rep 4. | 103 | 139 | 71.7 | 2.8e-18 |
| HS1_rep 5. | 140 | 176 | 26.6 | 0.0001 |
| --- cleavage 171 (inside HS1_rep 140..176) --- | ||||
| --- cleavage 181 --- | ||||
| --- cleavage 193 --- | ||||
| SH3_1 1. | 326 | 380 | 89.7 | 1e-23 |
3. Sequence Information
Fasta Sequence: XSB2166.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
3 [sites]
Cleavage sites (±10aa)
[Site 1] TVPIEAVTSK181-TSNIRANFEN
Lys181 
Thr
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Thr172 | Val173 | Pro174 | Ile175 | Glu176 | Ala177 | Val178 | Thr179 | Ser180 | Lys181 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr182 | Ser183 | Asn184 | Ile185 | Arg186 | Ala187 | Asn188 | Phe189 | Glu190 | Asn191 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 117.00 | 5 | Cttn protein |
| 2 | Rattus norvegicus | 117.00 | 4 | cortactin isoform B |
| 3 | N/A | 117.00 | 2 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 117.00 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 110.00 | 19 | cortactin isoform b |
| 6 | synthetic construct | 110.00 | 3 | cortactin |
| 7 | Macaca mulatta | 110.00 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Macaca fascicularis | 110.00 | 2 | unnamed protein product |
| 9 | Pongo pygmaeus | 108.00 | 1 | hypothetical protein |
| 10 | Bos taurus | 105.00 | 3 | cortactin |
| 11 | Canis familiaris | 104.00 | 6 | PREDICTED: similar to cortactin isoform b isoform |
| 12 | Gallus gallus | 91.30 | 2 | cortactin |
| 13 | Xenopus laevis | 85.90 | 4 | cortactin |
| 14 | Danio rerio | 72.00 | 12 | cortactin |
| 15 | Tetraodon nigroviridis | 71.60 | 4 | unnamed protein product |
| 16 | Xenopus tropicalis | 63.50 | 1 | cortactin |
| 17 | Aedes aegypti | 49.70 | 1 | cortactin |
| 18 | Pan troglodytes | 48.10 | 9 | PREDICTED: hypothetical protein isoform 2 |
| 19 | Strongylocentrotus purpuratus | 47.80 | 1 | SRC8 protein |
| 20 | Drosophila melanogaster | 39.30 | 2 | Cortactin CG3637-PA |
| 21 | Drosophila pseudoobscura | 38.10 | 1 | GA17577-PA |
| 22 | Monodelphis domestica | 35.80 | 2 | PREDICTED: similar to KIAA1561 protein |
| 23 | Parvibaculum lavamentivorans DS-1 | 35.80 | 1 | translation initiation factor IF-2 |
| 24 | Anopheles gambiae str. PEST | 33.50 | 1 | ENSANGP00000029554 |
| 25 | Neurospora crassa | 32.70 | 1 | related to putative cytoplasmic structural protein |
| 26 | Neurospora crassa OR74A | 32.70 | 1 | hypothetical protein |
| 27 | Arabidopsis thaliana | 32.30 | 2 | unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |
| Rattus norvegicus | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |
| N/A | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |
| Homo sapiens | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| synthetic construct | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| Macaca mulatta | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| Macaca fascicularis | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 1 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 60 |
| Pongo pygmaeus | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| || ||||||||+||| ||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVASK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| Bos taurus | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| | |+||||||+||| ||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVAAVSSSYQKTVPVEAVNSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| Canis familiaris | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||||||+| || |||||||+||| |+#||||||||||||||+|||||||||||+||| Sbjct 243 MDKNASTFEDVAQVAPAYQKTVPVEAVNSR#TSNIRANFENLAKEKEQEDRRKAEAEKAQR 302 |
| Gallus gallus | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||||+|||++ + | |||| |+| | +|#||+|||| ||||||+||||||||||||||| Sbjct 326 MDKNAATFEDIEKPTSTYQKTKPVERVANK#TSSIRANLENLAKEKEQEDRRKAEAERAQR 385 |
| Xenopus laevis | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ||| |++||+| +| |+|||| |+| ||# |+||||||||||++|||||+||||||||| Sbjct 315 MDKAAASFEDVEKVSSSYQKTRPVEVEGSK#ASSIRANFENLAKDKEQEDRKKAEAERAQR 374 |
| Danio rerio | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||+| ||||| + +||||| |+|| +| # |+|+| |||+||++|+|++++ | ||++| Sbjct 316 MDKSAGTFEEVQKPSAAYQKTRPVEAASSS#ASSIKARFENIAKQKEEEEQQRLEEERSRR 375 |
| Tetraodon nigroviridis | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ||| | ||||| + +|||| |+|| | # +|+| |||+||++| ||||+|| || +| Sbjct 20 MDKAAGTFEEVEKPTPSYQKTKPVEAAGSN#AGSIKARFENMAKQKEDEDRRRAEEERLRR 79 |
| Xenopus tropicalis | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 +|| || | |+ |||+|| |+||+|| # |+|+ |||+|+ |+|+++||| || +| Sbjct 278 VDKCASGFGEIETPSSAYEKTQPLEAMTSG#AHNLRSRFENMARTAEEENKQKAEEERVRR 337 |
| Aedes aegypti | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 |||+| |+| ++ + | || | + ++|# ||||| ||| | |+| |+||| ++ | Sbjct 241 MDKSALGFQEQDKIGTNYTKTKP-DIGSAK#PSNIRARFENFAMAAEEETRKKAEEQKRLR 299 |
| Pan troglodytes | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKE---REQEDRRKAEAER 208 +||+| | |+ +||+|| |||| +| # ++| ||++|+| ||+|++ + | | Sbjct 205 VDKSAVGFNEMEAPTTAYKKTTPIEAASSG#ARGLKAKFESMAEEKRKREEEEKAQQVARR 264 Query 209 AQ 210 | Sbjct 265 QQ 266 |
| Strongylocentrotus purpuratus | Query 153 DKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 | | | ++ | |+|+|| | | # |+| || +|+ |+| ||||| || +| Sbjct 281 DATAGGFGDMQGVSSSYKKTRPQPPAKSG#AGNMRNKFEQMAQAGEEESRRKAEEERGRR 339 |
| Drosophila melanogaster | Query 180 SK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 +|# ||+|| |||||| |+| |++|| ++ | Sbjct 238 AK#PSNLRAKFENLAKNSEEESRKRAEEQKRLR 269 |
| Drosophila pseudoobscura | Query 180 SK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 +|# ||+|| |||||| |+| |+++| ++ | Sbjct 236 AK#PSNLRAKFENLAKNSEEESRKRSEEQKRLR 267 |
| Monodelphis domestica | Query 152 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERA 209 +++|| ||+ + || ||+ | + # +++ + | | +| + + || ||+|| Sbjct 1146 LEENAKQNSEVLAAQALLQKHVPLGQVEAL#KNSLSSTIEALREELKDKQRRHEEAQRA 1203 |
| Parvibaculum lavamentivorans DS-1 | Query 162 VVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAE 207 | + || + | + +| +# | || + |+||| ||||+|| | Sbjct 118 VEKKPSRSRSGVVLRTLTEE#EKNARAQALDSAREREGEDRRRAEEE 163 |
| Anopheles gambiae str. PEST | Query 179 TSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 ++|# ||+|| ||| | |+| |++|+ ++ | Sbjct 237 SAK#PSNLRAKFENFAATAEEEARKRADEQKRLR 269 |
| Neurospora crassa | Query 160 EEVVQVPSAYQKTVPIEAV----------TSK#TSNIRANFENLAKER-EQEDRRKAEAER 208 || + || +|+|| +| | +# + +| | +|+|+ |+ +| ||| |+ Sbjct 1529 EETLYVPPQHQETVDTKAQEDQLKAQKVETER#LEHEKAEQERVAREKAERAEREKAEREK 1588 Query 209 AQR 211 |+| Sbjct 1589 AER 1591 |
| Neurospora crassa OR74A | Query 160 EEVVQVPSAYQKTVPIEAV----------TSK#TSNIRANFENLAKER-EQEDRRKAEAER 208 || + || +|+|| +| | +# + +| | +|+|+ |+ +| ||| |+ Sbjct 1452 EETLYVPPQHQETVDTKAQEDQLKAQKVETER#LEHEKAEQERVAREKAERAEREKAEREK 1511 Query 209 AQR 211 |+| Sbjct 1512 AER 1514 |
| Arabidopsis thaliana | Query 177 AVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 211 | |# | +|+ + + ||+|+|||++ || + +| Sbjct 736 ARADK#LRNFKADLQKMKKEKEEEDRKRIEALKIER 770 |
[Site 2] VVQVPSAYQK171-TVPIEAVTSK
Lys171 Thr
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val162 | Val163 | Gln164 | Val165 | Pro166 | Ser167 | Ala168 | Tyr169 | Gln170 | Lys171 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr172 | Val173 | Pro174 | Ile175 | Glu176 | Ala177 | Val178 | Thr179 | Ser180 | Lys181 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| GGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 120.00 | 5 | Cttn protein |
| 2 | Rattus norvegicus | 120.00 | 4 | cortactin isoform B |
| 3 | N/A | 120.00 | 2 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 120.00 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 113.00 | 10 | cortactin isoform b |
| 6 | synthetic construct | 113.00 | 3 | cortactin |
| 7 | Macaca mulatta | 113.00 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Pongo pygmaeus | 111.00 | 1 | hypothetical protein |
| 9 | Canis familiaris | 108.00 | 5 | PREDICTED: similar to cortactin isoform b isoform |
| 10 | Bos taurus | 108.00 | 2 | cortactin |
| 11 | Gallus gallus | 94.40 | 2 | cortactin |
| 12 | Macaca fascicularis | 92.40 | 2 | unnamed protein product |
| 13 | Xenopus laevis | 90.10 | 3 | cortactin |
| 14 | Danio rerio | 84.30 | 10 | cortactin |
| 15 | Tetraodon nigroviridis | 79.70 | 3 | unnamed protein product |
| 16 | Strongylocentrotus purpuratus | 48.10 | 1 | SRC8 protein |
| 17 | Drosophila melanogaster | 47.00 | 2 | Cortactin CG3637-PA |
| 18 | Drosophila pseudoobscura | 47.00 | 1 | GA17577-PA |
| 19 | Pan troglodytes | 43.50 | 5 | PREDICTED: cortactin isoform 2 |
| 20 | Tribolium castaneum | 42.40 | 1 | PREDICTED: similar to Xaa-Pro dipeptidase (X-Pro d |
| 21 | Monodelphis domestica | 39.30 | 1 | PREDICTED: hypothetical protein |
| 22 | Suberites domuncula | 35.40 | 1 | cortactin |
| 23 | Anopheles gambiae str. PEST | 33.50 | 1 | ENSANGP00000029554 |
| 24 | Xenopus tropicalis | 33.10 | 1 | cortactin |
| 25 | Aedes aegypti | 32.30 | 1 | cortactin |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |
| Rattus norvegicus | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |
| N/A | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |
| Homo sapiens | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 329 |
| synthetic construct | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 329 |
| Macaca mulatta | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 |
| Pongo pygmaeus | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||||||+| || |||||#|||+||| ||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVASKTSNIRANFENLAKEKEQEDR 329 |
| Canis familiaris | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||||||+| || ||||#|||+||| |+||||||||||||||+||||| Sbjct 233 GGKYGVQKDRMDKNASTFEDVAQVAPAYQK#TVPVEAVNSRTSNIRANFENLAKEKEQEDR 292 |
| Bos taurus | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||||||+| | |+|||#|||+||| ||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVAAVSSSYQK#TVPVEAVNSKTSNIRANFENLAKEKEQEDR 366 |
| Gallus gallus | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||||+|||++ + | |||#| |+| | +|||+|||| ||||||+||||| Sbjct 316 GGKYGVQKDRMDKNAATFEDIEKPTSTYQK#TKPVERVANKTSSIRANLENLAKEKEQEDR 375 |
| Macaca fascicularis | Query 152 MDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 1 MDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 50 |
| Xenopus laevis | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 ||||||||||||| |++||+| +| |+|||#| |+| || |+||||||||||++||||| Sbjct 305 GGKYGVQKDRMDKAAASFEDVEKVSSSYQK#TRPVEVEGSKASSIRANFENLAKDKEQEDR 364 |
| Danio rerio | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||||||||||||+| ||||| + +||||#| |+|| +| |+|+| |||+||++|+|++ Sbjct 306 GGKYGVQKDRMDKSAGTFEEVQKPSAAYQK#TRPVEAASSSASSIKARFENIAKQKEEEEQ 365 |
| Tetraodon nigroviridis | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||+||||||||| | ||||| + +|||#| |+|| | +|+| |||+||++| ||| Sbjct 10 GGKFGVQKDRMDKAAGTFEEVEKPTPSYQK#TKPVEAAGSNAGSIKARFENMAKQKEDEDR 69 |
| Strongylocentrotus purpuratus | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||+|| |+ | | | ++ | |+|+|#| | | |+| || +|+ |+| | Sbjct 270 GGKFGVDKNAQDATAGGFGDMQGVSSSYKK#TRPQPPAKSGAGNMRNKFEQMAQAGEEESR 329 |
| Drosophila melanogaster | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVT----SKTSNIRANFENLAKERE 197 |||+|||+|| ||+| ++ + | + # | + +| ||+|| |||||| | Sbjct 197 GGKFGVQEDRKDKSAVGWDHK-EAPQKHAS#QVDHKVKPVIEGAKPSNLRAKFENLAKNSE 255 Query 198 QEDR 201 +| | Sbjct 256 EESR 259 |
| Drosophila pseudoobscura | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVT----SKTSNIRANFENLAKERE 197 |||+|||+|| ||+| ++ + | + # | + +| ||+|| |||||| | Sbjct 195 GGKFGVQEDRKDKSAVGWDHK-EAPQKHAS#QVDHKVKPVIEGAKPSNLRAKFENLAKNSE 253 Query 198 QEDR 201 +| | Sbjct 254 EESR 257 |
| Pan troglodytes | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKT 182 |||||||||||||++ + + + | ||# |+ |+ + | Sbjct 270 GGKYGVQKDRMDKSSPSLGQTIVESPAMQK#GFPVAAIVTTT 310 |
| Tribolium castaneum | Query 142 GGKYGVQKDRMDKNASTF-EEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENL 192 |||+|+| ||+||+|+ | +| +| + | |# | + +| ++|| |||+ Sbjct 736 GGKFGLQTDRVDKSAANFNDEPAKVGTNYTK#VKP-DIGDAKPKDLRAKFENM 786 |
| Monodelphis domestica | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTS---KTSNIRANFENLAKEREQ 198 ||||||||||||| | | | |#+ + | | |++ | | + | Sbjct 270 GGKYGVQKDRMDKRGGTAEPQAQSGRPQTK#SSLLTLATGVPCSISGIKSAFSNHQTPQAQ 329 Query 199 ED 200 | Sbjct 330 SD 331 |
| Suberites domuncula | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLA 193 ||||||++ | +| ++++ | | ++ # + +| +||+ |||+| Sbjct 271 GGKYGVEEGNQDSSAGGYDDMQAVKSDHRT#ERGVS--KGQTGSIRSKFENMA 320 |
| Anopheles gambiae str. PEST | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAV------TSKTSNIRANFENLAKE 195 |||+||| || ||+| ++ |+ | ++ # + + | ++| ||+|| ||| | Sbjct 195 GGKFGVQTDRKDKSAFGWDH-VEKPQMHES#QLDHKIVIKPDIGSAKPSNLRAKFENFAAT 253 Query 196 REQEDR 201 |+| | Sbjct 254 AEEEAR 259 Query 142 GGKYGVQKDRMDKNASTFEEVVQV 165 |||+||+||||||+| | + +| Sbjct 84 GGKFGVEKDRMDKSAVGHEHIEKV 107 Query 142 GGKYGVQKDRMDKNASTFEEVVQV 165 |||+||||||+||+| ++ | +| Sbjct 121 GGKFGVQKDRVDKSAHGWDHVEKV 144 |
| Xenopus tropicalis | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 ||++||| ||+|| || | |+ |||+|#| |+||+|| |+|+ |||+|+ |+|++ Sbjct 268 GGRFGVQTDRVDKCASGFGEIETPSSAYEK#TQPLEAMTSGAHNLRSRFENMARTAEEENK 327 Query 142 GGKYGVQKDRMDKNASTFEEVVQV 165 |||||||||| ||+| |+ +| Sbjct 120 GGKYGVQKDRTDKSAVGFDYKAKV 143 Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQE 199 |||||||||| ||+| ++ | ++# | ++ | | | + |+|+ + Sbjct 194 GGKYGVQKDRQDKSAHSW-------SHKEE#LQPHQSQTDYTQGFGGKF-GVQKDRQDK 243 |
| Aedes aegypti | Query 142 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 201 |||+||| |||||+| |+| ++ + | |#| | + ++| ||||| ||| | |+| | Sbjct 231 GGKFGVQSDRMDKSALGFQEQDKIGTNYTK#TKP-DIGSAKPSNIRARFENFAMAAEEETR 289 Query 142 GGKYGVQKDRMDKNASTFEEVVQV 165 |||+||| ||+|| |+|+| +| Sbjct 120 GGKFGVQSDRVDKCAATWEHKEKV 143 |
[Site 3] NIRANFENLA193-KEREQEDRRK
Ala193 Lys
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Asn184 | Ile185 | Arg186 | Ala187 | Asn188 | Phe189 | Glu190 | Asn191 | Leu192 | Ala193 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Lys194 | Glu195 | Arg196 | Glu197 | Gln198 | Glu199 | Asp200 | Arg201 | Arg202 | Lys203 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| QVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEAR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 63.20 | 5 | unnamed protein product |
| 2 | Rattus norvegicus | 63.20 | 4 | cortactin isoform B |
| 3 | N/A | 63.20 | 1 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 63.20 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 59.70 | 10 | cortactin isoform b |
| 6 | synthetic construct | 59.70 | 3 | cortactin |
| 7 | Macaca mulatta | 59.70 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Macaca fascicularis | 59.70 | 2 | unnamed protein product |
| 9 | Pongo pygmaeus | 57.80 | 1 | hypothetical protein |
| 10 | Canis familiaris | 54.70 | 5 | PREDICTED: similar to cortactin isoform b isoform |
| 11 | Bos taurus | 54.70 | 2 | cortactin |
| 12 | Xenopus laevis | 43.90 | 3 | cortactin |
| 13 | Gallus gallus | 43.50 | 2 | cortactin |
| 14 | Danio rerio | 40.00 | 1 | cortactin |
| 15 | Xenopus tropicalis | 38.90 | 1 | cortactin |
| 16 | Tetraodon nigroviridis | 35.80 | 2 | unnamed protein product |
| 17 | Pan troglodytes | 32.30 | 2 | PREDICTED: hypothetical protein isoform 2 |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 ||||||||||||||||||||||||||||||#| Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 |
| Rattus norvegicus | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 ||||||||||||||||||||||||||||||#| Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 |
| N/A | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 ||||||||||||||||||||||||||||||#| Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 ||||||||||||||||||||||||||||||#| Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 |
| Homo sapiens | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 || ||||||||+||||||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 322 |
| synthetic construct | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 || ||||||||+||||||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 322 |
| Macaca mulatta | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 || ||||||||+||||||||||||||||||#| Sbjct 329 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 359 |
| Macaca fascicularis | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 || ||||||||+||||||||||||||||||#| Sbjct 13 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 43 |
| Pongo pygmaeus | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 || ||||||||+||| ||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVASKTSNIRANFENLA#K 322 |
| Canis familiaris | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 || |||||||+||| |+||||||||||||#| Sbjct 255 QVAPAYQKTVPVEAVNSRTSNIRANFENLA#K 285 |
| Bos taurus | Query 165 VPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 | |+||||||+||| ||||||||||||||#| Sbjct 330 VSSSYQKTVPVEAVNSKTSNIRANFENLA#K 359 |
| Xenopus laevis | Query 164 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 194 +| |+|||| |+| || |+|||||||||#| Sbjct 327 KVSSSYQKTRPVEVEGSKASSIRANFENLA#K 357 |
| Gallus gallus | Query 167 SAYQKTVPIEAVTSKTSNIRANFENLA#K 194 | |||| |+| | +|||+|||| ||||#| Sbjct 341 STYQKTKPVERVANKTSSIRANLENLA#K 368 |
| Danio rerio | Query 164 QVPSA-YQKTVPIEAVTSKTSNIRANFENLA#K 194 | ||| |||| |+|| +| |+|+| |||+|#| Sbjct 327 QKPSAAYQKTRPVEAASSSASSIKARFENIA#K 358 |
| Xenopus tropicalis | Query 167 SAYQKTVPIEAVTSKTSNIRANFENLA#K 194 |||+|| |+||+|| |+|+ |||+|#+ Sbjct 293 SAYEKTQPLEAMTSGAHNLRSRFENMA#R 320 |
| Tetraodon nigroviridis | Query 168 AYQKTVPIEAVTSKTSNIRANFENLA#K 194 +|||| |+|| | +|+| |||+|#| Sbjct 36 SYQKTKPVEAAGSNAGSIKARFENMA#K 62 |
| Pan troglodytes | Query 167 SAYQKTVPIEAVTSKTSNIRANFENLA#K 194 +||+|| |||| +| ++| ||++|#+ Sbjct 220 TAYKKTTPIEAASSGARGLKAKFESMA#E 247 |
* References
[PubMed ID: 15632090] Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M, Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail S, Li P, Sutton G, Gene and alternative splicing annotation with AIR. Genome Res. 2005 Jan;15(1):54-66.