XSB2168 : cortactin isoform B [Rattus norvegicus]
[ CaMP Format ]
This entry is computationally expanded from SB0022
* Basic Information
| Organism | Rattus norvegicus (Norway rat) |
| Protein Names | Cortactin; cortactin isoform B |
| Gene Names | Cttn; cortactin |
| Gene Locus | 1q41-q42; chromosome 1 |
| GO Function | Not available |
| Entrez Protein | Entrez Nucleotide | Entrez Gene | UniProt | OMIM | HGNC | HPRD | KEGG |
|---|---|---|---|---|---|---|---|
| NP_068640 | NM_021868 | 60465 | Q66HL2_RAT | N/A | N/A | N/A | rno:60465 |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 509 aa
Average Mass: 56.942 kDa
Monoisotopic Mass: 56.907 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| HS1_rep 1. | 83 | 119 | 68.2 | 3.1e-17 |
| HS1_rep 2. | 120 | 156 | 72.7 | 1.3e-18 |
| HS1_rep 3. | 157 | 193 | 74.7 | 3.4e-19 |
| HS1_rep 4. | 194 | 230 | 69.9 | 9.2e-18 |
| HS1_rep 5. | 231 | 267 | 71.7 | 2.8e-18 |
| HS1_rep 6. | 268 | 304 | 26.6 | 0.0001 |
| --- cleavage 299 (inside HS1_rep 268..304) --- | ||||
| --- cleavage 309 --- | ||||
| --- cleavage 321 --- | ||||
| SH3_1 1. | 454 | 508 | 89.7 | 1e-23 |
3. Sequence Information
Fasta Sequence: XSB2168.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
3 [sites]
Cleavage sites (±10aa)
[Site 1] TVPIEAVTSK309-TSNIRANFEN
Lys309 
Thr
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Thr300 | Val301 | Pro302 | Ile303 | Glu304 | Ala305 | Val306 | Thr307 | Ser308 | Lys309 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr310 | Ser311 | Asn312 | Ile313 | Arg314 | Ala315 | Asn316 | Phe317 | Glu318 | Asn319 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 117.00 | 5 | Cttn protein |
| 2 | Rattus norvegicus | 117.00 | 4 | cortactin isoform B |
| 3 | N/A | 117.00 | 2 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 117.00 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 110.00 | 19 | cortactin isoform b |
| 6 | synthetic construct | 110.00 | 3 | cortactin |
| 7 | Macaca mulatta | 110.00 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Macaca fascicularis | 110.00 | 2 | unnamed protein product |
| 9 | Pongo pygmaeus | 108.00 | 1 | hypothetical protein |
| 10 | Bos taurus | 105.00 | 3 | cortactin |
| 11 | Canis familiaris | 104.00 | 6 | PREDICTED: similar to cortactin isoform b isoform |
| 12 | Gallus gallus | 91.30 | 2 | cortactin |
| 13 | Xenopus laevis | 85.90 | 4 | cortactin |
| 14 | Danio rerio | 72.00 | 12 | cortactin |
| 15 | Tetraodon nigroviridis | 71.60 | 4 | unnamed protein product |
| 16 | Xenopus tropicalis | 63.50 | 1 | cortactin |
| 17 | Aedes aegypti | 49.70 | 1 | cortactin |
| 18 | Pan troglodytes | 48.10 | 9 | PREDICTED: hypothetical protein isoform 1 |
| 19 | Strongylocentrotus purpuratus | 47.80 | 1 | SRC8 protein |
| 20 | Drosophila melanogaster | 39.30 | 2 | Cortactin |
| 21 | Drosophila pseudoobscura | 38.10 | 1 | GA17577-PA |
| 22 | Monodelphis domestica | 35.80 | 2 | PREDICTED: similar to KIAA1561 protein |
| 23 | Parvibaculum lavamentivorans DS-1 | 35.80 | 1 | translation initiation factor IF-2 |
| 24 | Anopheles gambiae str. PEST | 33.50 | 1 | ENSANGP00000029554 |
| 25 | Neurospora crassa | 32.70 | 1 | related to putative cytoplasmic structural protein |
| 26 | Neurospora crassa OR74A | 32.70 | 1 | hypothetical protein |
| 27 | Arabidopsis thaliana | 32.30 | 2 | unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |
| Rattus norvegicus | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |
| N/A | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |
| Homo sapiens | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| synthetic construct | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| Macaca mulatta | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| Macaca fascicularis | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| || ||||||||+||||||#||||||||||||||+||||||||||||||| Sbjct 1 MDKNASTFEDVTQVSSAYQKTVPVEAVTSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 60 |
| Pongo pygmaeus | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| || ||||||||+||| ||#||||||||||||||+||||||||||||||| Sbjct 280 MDKNASTFEDVTQVSSAYQKTVPVEAVASK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 339 |
| Bos taurus | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| | |+||||||+||| ||#||||||||||||||+||||||||||||||| Sbjct 317 MDKNASTFEDVAAVSSSYQKTVPVEAVNSK#TSNIRANFENLAKEKEQEDRRKAEAERAQR 376 |
| Canis familiaris | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||||||+| || |||||||+||| |+#||||||||||||||+|||||||||||+||| Sbjct 280 MDKNASTFEDVAQVAPAYQKTVPVEAVNSR#TSNIRANFENLAKEKEQEDRRKAEAEKAQR 339 |
| Gallus gallus | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||||+|||++ + | |||| |+| | +|#||+|||| ||||||+||||||||||||||| Sbjct 326 MDKNAATFEDIEKPTSTYQKTKPVERVANK#TSSIRANLENLAKEKEQEDRRKAEAERAQR 385 |
| Xenopus laevis | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ||| |++||+| +| |+|||| |+| ||# |+||||||||||++|||||+||||||||| Sbjct 315 MDKAAASFEDVEKVSSSYQKTRPVEVEGSK#ASSIRANFENLAKDKEQEDRKKAEAERAQR 374 |
| Danio rerio | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||+| ||||| + +||||| |+|| +| # |+|+| |||+||++|+|++++ | ||++| Sbjct 316 MDKSAGTFEEVQKPSAAYQKTRPVEAASSS#ASSIKARFENIAKQKEEEEQQRLEEERSRR 375 |
| Tetraodon nigroviridis | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ||| | ||||| + +|||| |+|| | # +|+| |||+||++| ||||+|| || +| Sbjct 325 MDKAAGTFEEVEKPTPSYQKTKPVEAAGSN#AGSIKARFENMAKQKEDEDRRRAEEERLRR 384 |
| Xenopus tropicalis | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 +|| || | |+ |||+|| |+||+|| # |+|+ |||+|+ |+|+++||| || +| Sbjct 278 VDKCASGFGEIETPSSAYEKTQPLEAMTSG#AHNLRSRFENMARTAEEENKQKAEEERVRR 337 |
| Aedes aegypti | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 |||+| |+| ++ + | || | + ++|# ||||| ||| | |+| |+||| ++ | Sbjct 241 MDKSALGFQEQDKIGTNYTKTKP-DIGSAK#PSNIRARFENFAMAAEEETRKKAEEQKRLR 299 |
| Pan troglodytes | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKE---REQEDRRKAEAER 336 +||+| | |+ +||+|| |||| +| # ++| ||++|+| ||+|++ + | | Sbjct 246 VDKSAVGFNEMEAPTTAYKKTTPIEAASSG#ARGLKAKFESMAEEKRKREEEEKAQQVARR 305 Query 337 AQ 338 | Sbjct 306 QQ 307 |
| Strongylocentrotus purpuratus | Query 281 DKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 | | | ++ | |+|+|| | | # |+| || +|+ |+| ||||| || +| Sbjct 281 DATAGGFGDMQGVSSSYKKTRPQPPAKSG#AGNMRNKFEQMAQAGEEESRRKAEEERGRR 339 |
| Drosophila melanogaster | Query 308 SK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 +|# ||+|| |||||| |+| |++|| ++ | Sbjct 238 AK#PSNLRAKFENLAKNSEEESRKRAEEQKRLR 269 |
| Drosophila pseudoobscura | Query 308 SK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 +|# ||+|| |||||| |+| |+++| ++ | Sbjct 236 AK#PSNLRAKFENLAKNSEEESRKRSEEQKRLR 267 |
| Monodelphis domestica | Query 280 MDKNASTFEEVVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAERA 337 +++|| ||+ + || ||+ | + # +++ + | | +| + + || ||+|| Sbjct 1146 LEENAKQNSEVLAAQALLQKHVPLGQVEAL#KNSLSSTIEALREELKDKQRRHEEAQRA 1203 |
| Parvibaculum lavamentivorans DS-1 | Query 290 VVQVPSAYQKTVPIEAVTSK#TSNIRANFENLAKEREQEDRRKAEAE 335 | + || + | + +| +# | || + |+||| ||||+|| | Sbjct 118 VEKKPSRSRSGVVLRTLTEE#EKNARAQALDSAREREGEDRRRAEEE 163 |
| Anopheles gambiae str. PEST | Query 307 TSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 ++|# ||+|| ||| | |+| |++|+ ++ | Sbjct 237 SAK#PSNLRAKFENFAATAEEEARKRADEQKRLR 269 |
| Neurospora crassa | Query 288 EEVVQVPSAYQKTVPIEAV----------TSK#TSNIRANFENLAKER-EQEDRRKAEAER 336 || + || +|+|| +| | +# + +| | +|+|+ |+ +| ||| |+ Sbjct 1529 EETLYVPPQHQETVDTKAQEDQLKAQKVETER#LEHEKAEQERVAREKAERAEREKAEREK 1588 Query 337 AQR 339 |+| Sbjct 1589 AER 1591 |
| Neurospora crassa OR74A | Query 288 EEVVQVPSAYQKTVPIEAV----------TSK#TSNIRANFENLAKER-EQEDRRKAEAER 336 || + || +|+|| +| | +# + +| | +|+|+ |+ +| ||| |+ Sbjct 1452 EETLYVPPQHQETVDTKAQEDQLKAQKVETER#LEHEKAEQERVAREKAERAEREKAEREK 1511 Query 337 AQR 339 |+| Sbjct 1512 AER 1514 |
| Arabidopsis thaliana | Query 305 AVTSK#TSNIRANFENLAKEREQEDRRKAEAERAQR 339 | |# | +|+ + + ||+|+|||++ || + +| Sbjct 736 ARADK#LRNFKADLQKMKKEKEEEDRKRIEALKIER 770 |
[Site 2] VVQVPSAYQK299-TVPIEAVTSK
Lys299 Thr
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val290 | Val291 | Gln292 | Val293 | Pro294 | Ser295 | Ala296 | Tyr297 | Gln298 | Lys299 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr300 | Val301 | Pro302 | Ile303 | Glu304 | Ala305 | Val306 | Thr307 | Ser308 | Lys309 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| GGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 120.00 | 5 | Cttn protein |
| 2 | Rattus norvegicus | 120.00 | 4 | cortactin isoform B |
| 3 | N/A | 120.00 | 2 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 120.00 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 113.00 | 10 | cortactin isoform b |
| 6 | synthetic construct | 113.00 | 3 | cortactin |
| 7 | Macaca mulatta | 113.00 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Pongo pygmaeus | 111.00 | 1 | hypothetical protein |
| 9 | Canis familiaris | 108.00 | 5 | PREDICTED: similar to cortactin isoform b isoform |
| 10 | Bos taurus | 108.00 | 2 | cortactin |
| 11 | Gallus gallus | 94.40 | 2 | cortactin |
| 12 | Macaca fascicularis | 92.40 | 2 | unnamed protein product |
| 13 | Xenopus laevis | 90.10 | 3 | cortactin |
| 14 | Danio rerio | 84.30 | 10 | cortactin |
| 15 | Tetraodon nigroviridis | 79.70 | 3 | unnamed protein product |
| 16 | Strongylocentrotus purpuratus | 48.10 | 1 | SRC8 protein |
| 17 | Drosophila melanogaster | 47.00 | 2 | Cortactin CG3637-PA |
| 18 | Drosophila pseudoobscura | 47.00 | 1 | GA17577-PA |
| 19 | Pan troglodytes | 43.50 | 5 | PREDICTED: cortactin isoform 2 |
| 20 | Tribolium castaneum | 42.40 | 1 | PREDICTED: similar to Xaa-Pro dipeptidase (X-Pro d |
| 21 | Monodelphis domestica | 39.30 | 1 | PREDICTED: hypothetical protein |
| 22 | Suberites domuncula | 35.40 | 1 | cortactin |
| 23 | Anopheles gambiae str. PEST | 33.50 | 1 | ENSANGP00000029554 |
| 24 | Xenopus tropicalis | 33.10 | 1 | cortactin |
| 25 | Aedes aegypti | 32.30 | 1 | cortactin |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |
| Rattus norvegicus | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |
| N/A | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |
| Homo sapiens | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 329 |
| synthetic construct | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 329 |
| Macaca mulatta | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 366 |
| Pongo pygmaeus | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||||||+| || |||||#|||+||| ||||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVTQVSSAYQK#TVPVEAVASKTSNIRANFENLAKEKEQEDR 329 |
| Canis familiaris | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||||||+| || ||||#|||+||| |+||||||||||||||+||||| Sbjct 270 GGKYGVQKDRMDKNASTFEDVAQVAPAYQK#TVPVEAVNSRTSNIRANFENLAKEKEQEDR 329 |
| Bos taurus | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||||||+| | |+|||#|||+||| ||||||||||||||||+||||| Sbjct 307 GGKYGVQKDRMDKNASTFEDVAAVSSSYQK#TVPVEAVNSKTSNIRANFENLAKEKEQEDR 366 |
| Gallus gallus | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||||+|||++ + | |||#| |+| | +|||+|||| ||||||+||||| Sbjct 316 GGKYGVQKDRMDKNAATFEDIEKPTSTYQK#TKPVERVANKTSSIRANLENLAKEKEQEDR 375 |
| Macaca fascicularis | Query 280 MDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||+| || |||||#|||+||||||||||||||||||||+||||| Sbjct 1 MDKNASTFEDVTQVSSAYQK#TVPVEAVTSKTSNIRANFENLAKEKEQEDR 50 |
| Xenopus laevis | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 ||||||||||||| |++||+| +| |+|||#| |+| || |+||||||||||++||||| Sbjct 305 GGKYGVQKDRMDKAAASFEDVEKVSSSYQK#TRPVEVEGSKASSIRANFENLAKDKEQEDR 364 |
| Danio rerio | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||||||||||||+| ||||| + +||||#| |+|| +| |+|+| |||+||++|+|++ Sbjct 306 GGKYGVQKDRMDKSAGTFEEVQKPSAAYQK#TRPVEAASSSASSIKARFENIAKQKEEEEQ 365 |
| Tetraodon nigroviridis | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||+||||||||| | ||||| + +|||#| |+|| | +|+| |||+||++| ||| Sbjct 315 GGKFGVQKDRMDKAAGTFEEVEKPTPSYQK#TKPVEAAGSNAGSIKARFENMAKQKEDEDR 374 |
| Strongylocentrotus purpuratus | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||+|| |+ | | | ++ | |+|+|#| | | |+| || +|+ |+| | Sbjct 270 GGKFGVDKNAQDATAGGFGDMQGVSSSYKK#TRPQPPAKSGAGNMRNKFEQMAQAGEEESR 329 |
| Drosophila melanogaster | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVT----SKTSNIRANFENLAKERE 325 |||+|||+|| ||+| ++ + | + # | + +| ||+|| |||||| | Sbjct 197 GGKFGVQEDRKDKSAVGWDHK-EAPQKHAS#QVDHKVKPVIEGAKPSNLRAKFENLAKNSE 255 Query 326 QEDR 329 +| | Sbjct 256 EESR 259 |
| Drosophila pseudoobscura | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVT----SKTSNIRANFENLAKERE 325 |||+|||+|| ||+| ++ + | + # | + +| ||+|| |||||| | Sbjct 195 GGKFGVQEDRKDKSAVGWDHK-EAPQKHAS#QVDHKVKPVIEGAKPSNLRAKFENLAKNSE 253 Query 326 QEDR 329 +| | Sbjct 254 EESR 257 |
| Pan troglodytes | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKT 310 |||||||||||||++ + + + | ||# |+ |+ + | Sbjct 270 GGKYGVQKDRMDKSSPSLGQTIVESPAMQK#GFPVAAIVTTT 310 |
| Tribolium castaneum | Query 270 GGKYGVQKDRMDKNASTF-EEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENL 320 |||+|+| ||+||+|+ | +| +| + | |# | + +| ++|| |||+ Sbjct 736 GGKFGLQTDRVDKSAANFNDEPAKVGTNYTK#VKP-DIGDAKPKDLRAKFENM 786 |
| Monodelphis domestica | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTS---KTSNIRANFENLAKEREQ 326 ||||||||||||| | | | |#+ + | | |++ | | + | Sbjct 270 GGKYGVQKDRMDKRGGTAEPQAQSGRPQTK#SSLLTLATGVPCSISGIKSAFSNHQTPQAQ 329 Query 327 ED 328 | Sbjct 330 SD 331 |
| Suberites domuncula | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLA 321 ||||||++ | +| ++++ | | ++ # + +| +||+ |||+| Sbjct 271 GGKYGVEEGNQDSSAGGYDDMQAVKSDHRT#ERGVS--KGQTGSIRSKFENMA 320 |
| Anopheles gambiae str. PEST | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAV------TSKTSNIRANFENLAKE 323 |||+||| || ||+| ++ |+ | ++ # + + | ++| ||+|| ||| | Sbjct 195 GGKFGVQTDRKDKSAFGWDH-VEKPQMHES#QLDHKIVIKPDIGSAKPSNLRAKFENFAAT 253 Query 324 REQEDR 329 |+| | Sbjct 254 AEEEAR 259 Query 270 GGKYGVQKDRMDKNASTFEEVVQV 293 |||+||+||||||+| | + +| Sbjct 84 GGKFGVEKDRMDKSAVGHEHIEKV 107 Query 270 GGKYGVQKDRMDKNASTFEEVVQV 293 |||+||||||+||+| ++ | +| Sbjct 121 GGKFGVQKDRVDKSAHGWDHVEKV 144 |
| Xenopus tropicalis | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 ||++||| ||+|| || | |+ |||+|#| |+||+|| |+|+ |||+|+ |+|++ Sbjct 268 GGRFGVQTDRVDKCASGFGEIETPSSAYEK#TQPLEAMTSGAHNLRSRFENMARTAEEENK 327 Query 270 GGKYGVQKDRMDKNASTFEEVVQV 293 |||||||||| ||+| |+ +| Sbjct 120 GGKYGVQKDRTDKSAVGFDYKAKV 143 Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQE 327 |||||||||| ||+| ++ | ++# | ++ | | | + |+|+ + Sbjct 194 GGKYGVQKDRQDKSAHSW-------SHKEE#LQPHQSQTDYTQGFGGKF-GVQKDRQDK 243 |
| Aedes aegypti | Query 270 GGKYGVQKDRMDKNASTFEEVVQVPSAYQK#TVPIEAVTSKTSNIRANFENLAKEREQEDR 329 |||+||| |||||+| |+| ++ + | |#| | + ++| ||||| ||| | |+| | Sbjct 231 GGKFGVQSDRMDKSALGFQEQDKIGTNYTK#TKP-DIGSAKPSNIRARFENFAMAAEEETR 289 Query 270 GGKYGVQKDRMDKNASTFEEVVQV 293 |||+||| ||+|| |+|+| +| Sbjct 120 GGKFGVQSDRVDKCAATWEHKEKV 143 |
[Site 3] NIRANFENLA321-KEREQEDRRK
Ala321 Lys
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Asn312 | Ile313 | Arg314 | Ala315 | Asn316 | Phe317 | Glu318 | Asn319 | Leu320 | Ala321 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Lys322 | Glu323 | Arg324 | Glu325 | Gln326 | Glu327 | Asp328 | Arg329 | Arg330 | Lys331 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| QVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEAR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Mus musculus | 63.20 | 5 | unnamed protein product |
| 2 | Rattus norvegicus | 63.20 | 4 | cortactin isoform B |
| 3 | N/A | 63.20 | 1 | S |
| 4 | mice, BALB/c 3T3 cells, Peptide, 546 aa | 63.20 | 1 | cortactin, p80/p85 |
| 5 | Homo sapiens | 59.70 | 10 | cortactin isoform b |
| 6 | synthetic construct | 59.70 | 3 | cortactin |
| 7 | Macaca mulatta | 59.70 | 2 | PREDICTED: similar to cortactin isoform a |
| 8 | Macaca fascicularis | 59.70 | 2 | unnamed protein product |
| 9 | Pongo pygmaeus | 57.80 | 1 | hypothetical protein |
| 10 | Canis familiaris | 54.70 | 5 | PREDICTED: similar to cortactin isoform b isoform |
| 11 | Bos taurus | 54.70 | 2 | cortactin |
| 12 | Xenopus laevis | 43.90 | 3 | cortactin |
| 13 | Gallus gallus | 43.50 | 2 | cortactin |
| 14 | Danio rerio | 40.00 | 1 | cortactin |
| 15 | Xenopus tropicalis | 38.90 | 1 | cortactin |
| 16 | Tetraodon nigroviridis | 35.80 | 2 | unnamed protein product |
| 17 | Pan troglodytes | 32.30 | 2 | PREDICTED: hypothetical protein isoform 1 |
Top-ranked sequences
| organism | matching |
|---|---|
| Mus musculus | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 ||||||||||||||||||||||||||||||#| Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 |
| Rattus norvegicus | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 ||||||||||||||||||||||||||||||#| Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 |
| N/A | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 ||||||||||||||||||||||||||||||#| Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 |
| mice, BALB/c 3T3 cells, Peptide, 546 aa | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 ||||||||||||||||||||||||||||||#| Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 |
| Homo sapiens | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 || ||||||||+||||||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 322 |
| synthetic construct | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 || ||||||||+||||||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 322 |
| Macaca mulatta | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 || ||||||||+||||||||||||||||||#| Sbjct 329 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 359 |
| Macaca fascicularis | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 || ||||||||+||||||||||||||||||#| Sbjct 13 QVSSAYQKTVPVEAVTSKTSNIRANFENLA#K 43 |
| Pongo pygmaeus | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 || ||||||||+||| ||||||||||||||#| Sbjct 292 QVSSAYQKTVPVEAVASKTSNIRANFENLA#K 322 |
| Canis familiaris | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 || |||||||+||| |+||||||||||||#| Sbjct 292 QVAPAYQKTVPVEAVNSRTSNIRANFENLA#K 322 |
| Bos taurus | Query 293 VPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 | |+||||||+||| ||||||||||||||#| Sbjct 330 VSSSYQKTVPVEAVNSKTSNIRANFENLA#K 359 |
| Xenopus laevis | Query 292 QVPSAYQKTVPIEAVTSKTSNIRANFENLA#K 322 +| |+|||| |+| || |+|||||||||#| Sbjct 327 KVSSSYQKTRPVEVEGSKASSIRANFENLA#K 357 |
| Gallus gallus | Query 295 SAYQKTVPIEAVTSKTSNIRANFENLA#K 322 | |||| |+| | +|||+|||| ||||#| Sbjct 341 STYQKTKPVERVANKTSSIRANLENLA#K 368 |
| Danio rerio | Query 292 QVPSA-YQKTVPIEAVTSKTSNIRANFENLA#K 322 | ||| |||| |+|| +| |+|+| |||+|#| Sbjct 327 QKPSAAYQKTRPVEAASSSASSIKARFENIA#K 358 |
| Xenopus tropicalis | Query 295 SAYQKTVPIEAVTSKTSNIRANFENLA#K 322 |||+|| |+||+|| |+|+ |||+|#+ Sbjct 293 SAYEKTQPLEAMTSGAHNLRSRFENMA#R 320 |
| Tetraodon nigroviridis | Query 296 AYQKTVPIEAVTSKTSNIRANFENLA#K 322 +|||| |+|| | +|+| |||+|#| Sbjct 341 SYQKTKPVEAAGSNAGSIKARFENMA#K 367 |
| Pan troglodytes | Query 295 SAYQKTVPIEAVTSKTSNIRANFENLA#K 322 +||+|| |||| +| ++| ||++|#+ Sbjct 261 TAYKKTTPIEAASSGARGLKAKFESMA#E 288 |
* References
[PubMed ID: 18768925] Kruchten AE, Krueger EW, Wang Y, McNiven MA, Distinct phospho-forms of cortactin differentially regulate actin polymerization and focal adhesions. Am J Physiol Cell Physiol. 2008 Nov;295(5):C1113-22. Epub 2008 Sep 3.
[PubMed ID: 18353971] ... Singh VP, McNiven MA, Src-mediated cortactin phosphorylation regulates actin localization and injurious blebbing in acinar cells. Mol Biol Cell. 2008 May;19(5):2339-47. Epub 2008 Mar 19.
[PubMed ID: 17959830] ... Llado A, Timpson P, Vila de Muga S, Moreto J, Pol A, Grewal T, Daly RJ, Enrich C, Tebar F, Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin. Mol Biol Cell. 2008 Jan;19(1):17-29. Epub 2007 Oct 24.
[PubMed ID: 16533564] ... Davis CJ, Meighan PC, Taishi P, Krueger JM, Harding JW, Wright JW, REM sleep deprivation attenuates actin-binding protein cortactin: a link between sleep and hippocampal plasticity. Neurosci Lett. 2006 Jun 12;400(3):191-6. Epub 2006 Mar 14.
[PubMed ID: 16162656] ... Zhou S, Webb BA, Eves R, Mak AS, Effects of tyrosine phosphorylation of cortactin on podosome formation in A7r5 vascular smooth muscle cells. Am J Physiol Cell Physiol. 2006 Feb;290(2):C463-71. Epub 2005 Sep 14.
[PubMed ID: 15159385] ... Ortiz DF, Moseley J, Calderon G, Swift AL, Li S, Arias IM, Identification of HAX-1 as a protein that binds bile salt export protein and regulates its abundance in the apical membrane of Madin-Darby canine kidney cells. J Biol Chem. 2004 Jul 30;279(31):32761-70. Epub 2004 May 24.
[PubMed ID: 15060019] ... Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ, High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence. Genome Res. 2004 Apr;14(4):750-7.
[PubMed ID: 12612086] ... Cao H, Orth JD, Chen J, Weller SG, Heuser JE, McNiven MA, Cortactin is a component of clathrin-coated pits and participates in receptor-mediated endocytosis. Mol Cell Biol. 2003 Mar;23(6):2162-70.
[PubMed ID: 12151401] ... Hattan D, Nesti E, Cachero TG, Morielli AD, Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactin. J Biol Chem. 2002 Oct 11;277(41):38596-606. Epub 2002 Jul 31.
[PubMed ID: 11583995] ... Okamoto PM, Gamby C, Wells D, Fallon J, Vallee RB, Dynamin isoform-specific interaction with the shank/ProSAP scaffolding proteins of the postsynaptic density and actin cytoskeleton. J Biol Chem. 2001 Dec 21;276(51):48458-65. Epub 2001 Oct 2.