CaMPDB :: Substrate

SB0001 : CAPN2, m-calpain catalytic subunit, mCL

* Basic Information

Organism	Homo sapiens (human)
Protein Names	calpain 2, large subunit [Homo sapiens]; calpain 2, large subunit; calpain, large polypeptide L2; calcium-activated neutral proteinase; Calpain 2, large [catalytic] subunit; Calpain-2 catalytic subunit; 3.4.22.53; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain
Gene Names	CAPN2; CANPL2; calpain 2, (m/II) large subunit
Gene Locus	1q41-q42; chromosome 1
GO Function	GO:0004198 - calpain activity [Evidence IEA]; GO:0005509 - calcium ion binding [Evidence IEA]

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_001739	NM_001748	824	P17655	114230	HGNC:1479	00254	hsa:824

* Information From OMIM

Description: The calpains, or calcium-activated neutral proteases (EC 3.4.22.17), are nonlysosomal intracellular cysteine proteases. The mammalian calpains include 2 ubiquitous isoforms, calpain I (mu-calpain) and calpain II (m-calpain), 2 stomach-specific proteins, and CAPN3 (OMIM:114240), which is muscle-specific. Calpain I and calpain II are heterodimers with distinct large subunits, encoded by the CAPN1 (OMIM:114220) and CAPN2 genes, respectively, associated with a common small subunit (CAPNS1; OMIM:114170).

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* Structure Information

1. Primary Information

Length: 700 aa

Average Mass: 80.006 kDa

Monoisotopic Mass: 79.956 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 9 ---
--- cleavage 19 ---
Calpain family cysteine protease 1.	46	342	1.0	0.0
Calpain large subunit, domain III 1.	356	506	1.0	0.0
EF-hand domain pair 1.	552	598	20.0	0.0
EF-hand domain pair 2.	610	621	31.0	0.0
EF hand 1.	657	668	17.0	0.0
EF hand 2.	675	680	4.0	0.0

3. Sequence Information

Fasta Sequence: SB0001.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1KFU (X-ray; 250 A; L=2-700), 1KFX (X-ray; 315 A; L=2-700), 2NQA (X-ray; 220 A; A/B=48-346)

* Cleavage Information

2 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 8482370] Brown N, Crawford C, Structural modifications associated with the change in Ca2+ sensitivity on activation of m-calpain. FEBS Lett. 1993 May 3;322(1):65-8.

Cleavage sites (±10aa)

[Site 1] MAGIAAKLA⁹-KDREAAEGLG

Ala⁹ Lys

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	Met¹	Ala²	Gly³	Ile⁴	Ala⁵	Ala⁶	Lys⁷	Leu⁸	Ala⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNEC

Summary

#	organism	max score	hits	top seq
1	N/A	82.40	11	neutral protease large subunit
2	Homo sapiens	82.40	2	calpain 2, large subunit
3	Homo sapiens	82.00	6	AF261089_1 calpain large polypeptide L2
4	Macaca mulatta	80.50	2	PREDICTED: calpain 2, large subunit
5	Rattus norvegicus	80.10	5	calpain 2
6	Mus musculus	79.30	14	m-calpain large subunit
7	Xenopus tropicalis	59.70	4	calpain 2, (m/II) large subunit
8	Gallus gallus	58.90	1	calpain 1, (mu/I) large subunit
9	Coturnix coturnix	56.20	1	quail calpain
10	Xenopus laevis	55.80	4	calpain 8
11	Canis familiaris	55.10	3	PREDICTED: similar to calpain 8
12	Stizostedion vitreum vitreum	54.30	1	putative calpain-like protein
13	Oncorhynchus mykiss	52.80	1	m-calpain
14	Danio rerio	52.00	6	calpain 2, (m/II) large subunit a
15	Xenopus laevis	51.60	2	mu/m-calpain large subunit
16	Tetraodon nigroviridis	50.10	6	unnamed protein product
17	Rattus norvegicus	49.30	1	calpain 1, large subunit
18	Bos taurus	48.10	2	calpain 1, (mu/I) large subunit
19	synthetic construct	48.10	1	Homo sapiens calpain 1, (mu/I) large subunit
20	Pongo pygmaeus	47.80	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
21	Sus scrofa	46.60	1	calpain 1, large subunit
22	Gallus gallus	43.90	1	mu-calpain large subunit
23	Canis familiaris	37.00	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	36.20	1	calpain 1, large subunit

Top-ranked sequences

organism	matching
N/A	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Homo sapiens	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Homo sapiens	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Macaca mulatta	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|\|\|\|\| #\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLV#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39
Rattus norvegicus	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAMKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Mus musculus	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|\| \|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAIKLA#KDREAAEGLGSHERAIKYLNQDYETLRNEC 39
Xenopus tropicalis	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+\|+\| +\|\|#\|+\| \| \|\|\|++\|+\|+ \|\|+\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSGVADRLA#KERALASGLGTNEKALPYLSQDYEALRNEC 39
Gallus gallus	Query 2 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|+\| #+\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\|\|\|+ \|\| Sbjct 5 GGIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQEC 42
Coturnix coturnix	Query 2 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|\|+\| #+\|\| \|\|\|+\| \| \|+\|\|\|\|\|\|\|\|\|\|+ \| Sbjct 5 GGIAARLQ#RDRLRAEGVGEHNNAVKYLNQDYEALKQAC 42
Xenopus laevis	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+\|\|\|+\|+\|#\|\|\| \|+\|\|\|++++ +\|\|\|\|\|\|\|\| \|+ \| Sbjct 1 MSGIASKVA#KDRVLAQGLGTNDKPLKYLNQDYEQLKARC 39
Canis familiaris	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+ +\|\|++\|#\| + \|\| \|\|\|\|\|++\|+\|\|\| \|\|+\| \|\| +\| Sbjct 94 MSALAARIA#KQQAAAGGLGSHQKAVKYLGQDFEKLRQQC 132
Stizostedion vitreum vitreum	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+\|\|\|\| \| #+ \|\| +\|+\|\| +\|+\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 1 MSGIAATLQ#RRREKEQGIGSCSQAVKYLNQDYEALRRRC 39
Oncorhynchus mykiss	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+\|+\|+ \|\|#\| \| \| \| \|++ \|+\|\|\|\|\|\|+\|\|\|\| +\| Sbjct 1 MSGMASNLA#KKRALAAGFGTNANAVKYLNQDFEALRAQC 39
Danio rerio	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+\|+\|+ \|\|#\| \| \| \| \|++ \|+\|\|\|\|\|++\| \|\|+\|\| Sbjct 1 MSGVASTLA#KKRALAAGFGTNSNAVKYLNQNFETLRSEC 39
Xenopus laevis	Query 2 AGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|\|\|+\|\| # \|\| \| \|+\|\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\| Sbjct 5 GGIASKLK#IDRLKASGVGSHDCAVKYQNQDYESLKQQC 42
Tetraodon nigroviridis	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|+\|\|\|\|\|\| # \|+ \|+\| \|+ +\| \|\|\|\|\|\|+\|+\|\| \| Sbjct 1 MSGIAAKLQ#HHRDRAQGFGTASQAQKYLNQDFESLRESC 39
Rattus norvegicus	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|++\|++ #\| \|+ \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRDKELGLGRHENAIKYLGQDYENLRARC 49
Bos taurus	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|++\|++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRAKELGLGRHENAIKYLGQDYEQLRVHC 49
synthetic construct	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|++\|++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
Pongo pygmaeus	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|++\|++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KQRARELGLGRHENAIKYLGQDYEQLRVRC 49
Sus scrofa	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|++\|++ #\| \| \|\|\| \|\| \|\|\|\|\| \|\|\|\| \|\| \| Sbjct 13 GVSAQVQ#KLRAKELGLGRHENAIKYLGQDYEQLRAHC 49
Gallus gallus	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 \|++\|++ #+ \| \| \|\|\| \|+ \|+++ \|\|\| \|\|\|++\| Sbjct 13 GVSAQVQ#RQRAKALGLGQHQNAVRFRGQDYAALRDDC 49
Canis familiaris	Query 1 MAGIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNEC 39 + \|+\|\| ++# \| \|+\|\|\| \| \| \| \|\|++\| \|+ \| Sbjct 83 LEGMAAHIS#HSRLKAKGLGQHHNAQNYNNQNFEDLQAAC 121
Danio rerio	Query 3 GIAAKLA#KDREAAEGLGSHERAIKYLNQDYEALRNE 38 \|+\|\|+\| # + \|\|\| + \|+\|+\| \|\|\|\| \|\| + Sbjct 8 GMAARLR#SQWDRDAGLGQNHNAVKFLGQDYETLRAQ 43

[Site 2] KDREAAEGLG¹⁹-SHERAIKYLN

Gly¹⁹ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys¹⁰	Asp¹¹	Arg¹²	Glu¹³	Ala¹⁴	Ala¹⁵	Glu¹⁶	Gly¹⁷	Leu¹⁸	Gly¹⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser²⁰	His²¹	Glu²²	Arg²³	Ala²⁴	Ile²⁵	Lys²⁶	Tyr²⁷	Leu²⁸	Asn²⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDP

Summary

#	organism	max score	hits	top seq
1	N/A	102.00	12	neutral protease large subunit
2	Homo sapiens	102.00	7	AF261089_1 calpain large polypeptide L2
3	Homo sapiens	102.00	2	calpain 2, large subunit
4	Macaca mulatta	100.00	2	PREDICTED: calpain 2, large subunit
5	Rattus norvegicus	98.60	10	calpain 2
6	Mus musculus	97.10	23	calpain 2
7	Gallus gallus	75.50	2	calpain 1, (mu/I) large subunit
8	Xenopus tropicalis	73.20	5	calpain 2, (m/II) large subunit
9	Coturnix coturnix	71.20	1	quail calpain
10	Canis familiaris	70.10	6	PREDICTED: similar to calpain 8
11	Xenopus laevis	69.30	5	calpain 8
12	Xenopus laevis	66.20	2	mu/m-calpain large subunit
13	Danio rerio	65.10	6	PREDICTED: similar to Calpain 1, (mu/I) large sub
14	Stizostedion vitreum vitreum	65.10	1	putative calpain-like protein
15	Oncorhynchus mykiss	64.70	1	m-calpain
16	Tetraodon nigroviridis	63.50	12	unnamed protein product
17	synthetic construct	61.60	1	Homo sapiens calpain 1, (mu/I) large subunit
18	Rattus norvegicus	61.60	1	calpain 1, large subunit
19	Pongo pygmaeus	61.20	1	CAN1_PONPY Calpain-1 catalytic subunit (Calpain-1
20	Bos taurus	58.90	6	micromolar calcium activated neutral protease 1
21	Sus scrofa	58.90	2	calpain 1, large subunit
22	Gallus gallus	55.50	1	mu-calpain large subunit
23	Canis familiaris	47.40	1	PREDICTED: similar to Calpain-11 (Calcium-activat
24	Danio rerio	45.10	1	calpain 1, large subunit
25	Oryctolagus cuniculus	35.00	1	calpain 3, (p94)
26	Drosophila melanogaster	34.30	5	CAA55297.1
27	Drosophila melanogaster	34.30	3	Calpain-A CG7563-PB, isoform B
28	Caenorhabditis elegans	31.60	2	CaLPain family member (clp-1)

Top-ranked sequences

organism	matching
N/A	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Homo sapiens	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Homo sapiens	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Macaca mulatta	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MAGIAAKLVKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49
Rattus norvegicus	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAMKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
Mus musculus	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MAGIAIKLAKDREAAEGLG#SHERAIKYLNQDYETLRNECLEAGALFQDP 49
Gallus gallus	Query 2 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|+\| +\|\| \|\|\|+\|# \| \|+\|\|\|\|\|\|\|\|\|\|+ \|\|+\|+\|\|\|\|+\|\| Sbjct 5 GGIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQECIESGTLFRDP 52
Xenopus tropicalis	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|+\|+\| +\|\|\|+\| \| \|\|\|#++\|+\|+ \|\|+\|\|\|\|\|\|\|\|\|\|\|++\|\|\|\|+\| Sbjct 1 MSGVADRLAKERALASGLG#TNEKALPYLSQDYEALRNECLQSGTLFKD 48
Coturnix coturnix	Query 2 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|\|+\| +\|\| \|\|\|+\|# \| \|+\|\|\|\|\|\|\|\|\|\|+ \|+\|+\| \|\|+\|\| Sbjct 5 GGIAARLQRDRLRAEGVG#EHNNAVKYLNQDYEALKQACIESGALFRDP 52
Canis familiaris	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|+ +\|\|++\|\| + \|\| \|\|\|#\|\|++\|+\|\|\| \|\|+\| \|\| +\|\|+\|\| \|\|+\|\| Sbjct 94 MSALAARIAKQQAAAGGLG#SHQKAVKYLGQDFEKLRQQCLDAGVLFKDP 142
Xenopus laevis	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|+\|\|\|+\|+\|\|\|\| \|+\|\|\|#++++ +\|\|\|\|\|\|\|\| \|+ \|\|\|+ \|\|\|+\| Sbjct 1 MSGIASKVAKDRVLAQGLG#TNDKPLKYLNQDYEQLKARCLESNTLFED 48
Xenopus laevis	Query 2 AGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|\|\|+\|\| \|\| \| \|+\|#\|\|+ \|+\|\| \|\|\|\|\|+\|+ +\|+\|+\| \|\|+\|\| Sbjct 5 GGIASKLKIDRLKASGVG#SHDCAVKYQNQDYESLKQQCVESGILFEDP 52
Danio rerio	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 + \|++ ++ \|+\| \|\|\|+\|#+++ \|\|\|+\|\|\|\|\|\| \|\| \|\|+\|+\| \|\|+\|\| Sbjct 4 IGGVSTRIYKERLQAEGMG#ANDLAIKFLNQDYEELRRECVESGRLFEDP 52
Stizostedion vitreum vitreum	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|+\|\|\|\| \| + \|\| +\|+\|#\| +\|+\|\|\|\|\|\|\|\|\|\|\| \|\|\|+ +\|\|+\| Sbjct 1 MSGIAATLQRRREKEQGIG#SCSQAVKYLNQDYEALRRRCLESESLFKD 48
Oncorhynchus mykiss	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|+\|+\|+ \|\|\| \| \| \| \|#++ \|+\|\|\|\|\|\|+\|\|\|\| +\|\| \| \|\| \|\| Sbjct 1 MSGMASNLAKKRALAAGFG#TNANAVKYLNQDFEALRAQCLNRGGLFSDP 49
Tetraodon nigroviridis	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|+\|\|\|\|\|\| \|+ \|+\| \|#+ +\| \|\|\|\|\|\|+\|+\|\| \|\|\| \| \|\|\|\| Sbjct 1 MSGIAAKLQHHRDRAQGFG#TASQAQKYLNQDFESLRESCLERGELFQD 48
synthetic construct	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|++\|++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\|\|\|\|+\| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
Rattus norvegicus	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|++\|++ \| \|+ \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ \| \|\|\|\| Sbjct 13 GVSAQVQKQRDKELGLG#RHENAIKYLGQDYENLRARCLQNGVLFQD 58
Pongo pygmaeus	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|++\|++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\|\|\|\|+\| Sbjct 13 GVSAQVQKQRARELGLG#RHENAIKYLGQDYEQLRVRCLQSGTLFRD 58
Bos taurus	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|++\|++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|+ \| \|\|+\| Sbjct 13 GVSAQVQKQRAKELGLG#RHENAIKYLGQDYEQLRVHCLQRGALFRD 58
Sus scrofa	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|++\|++ \| \| \|\|\|# \|\| \|\|\|\|\| \|\|\|\| \|\| \|\|++\|+\|\|+\| Sbjct 13 GVSAQVQKLRAKELGLG#RHENAIKYLGQDYEQLRAHCLQSGSLFRD 58
Gallus gallus	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 \|++\|++ + \| \| \|\|\|# \|+ \|+++ \|\|\| \|\|\|++\|\| +\|+\|\|+\| Sbjct 13 GVSAQVQRQRAKALGLG#QHQNAVRFRGQDYAALRDDCLRSGSLFRD 58
Canis familiaris	Query 1 MAGIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 + \|+\|\| ++ \| \|+\|\|\|# \| \| \| \|\|++\| \|+ \|\| \| \|\|+\| Sbjct 83 LEGMAAHISHSRLKAKGLG#QHHNAQNYNNQNFEDLQAACLRKGELFED 130
Danio rerio	Query 3 GIAAKLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQDP 49 \|+\|\|+\| + \|\|\|# + \|+\|+\| \|\|\|\| \|\| + ++ \|\|+\|\| Sbjct 8 GMAARLRSQWDRDAGLG#QNHNAVKFLGQDYETLRAQSQQSRRLFEDP 54
Oryctolagus cuniculus	Query 7 KLAKDREAAEGLG#SHERAIKYLNQDYEALRNECLEAGTLFQD 48 ++ ++\|+ \| \|#+ + \|\|\| \|\|+ \|\| \|\| \|\|+\| Sbjct 12 RVIRERDRRNGEG#TVTQPIKYEGQDFVVLRQRCLAQKCLFED 53
Drosophila melanogaster	Query 30 QDYEALRNECLEAGTLFQDP 49 \|\|\|\| + \| \|\| +\|+\|\|+\|\| Sbjct 50 QDYETILNSCLASGSLFEDP 69
Drosophila melanogaster	Query 30 QDYEALRNECLEAGTLFQDP 49 \|\|\|\| + \| \|\| +\|+\|\|+\|\| Sbjct 73 QDYETILNSCLASGSLFEDP 92
Caenorhabditis elegans	Query 30 QDYEALRNECLEAGTLFQDP 49 \|\|+ \|\|++\|\|\|+ \|\|+\|\| Sbjct 255 QDFYELRDQCLESKRLFEDP 274

* References

[PubMed ID: 17359359] Medana IM, Day NP, Hien TT, Mai NT, Bethell D, Phu NH, Turner GD, Farrar J, White NJ, Esiri MM, Cerebral calpain in fatal falciparum malaria. Neuropathol Appl Neurobiol. 2007 Apr;33(2):179-92.

» More (10) « Hide Below

[PubMed ID: 17192410] ... Nuzzi PA, Senetar MA, Huttenlocher A, Asymmetric localization of calpain 2 during neutrophil chemotaxis. Mol Biol Cell. 2007 Mar;18(3):795-805. Epub 2006 Dec 27.

[PubMed ID: 16652152] ... Carragher NO, Walker SM, Scott Carragher LA, Harris F, Sawyer TK, Brunton VG, Ozanne BW, Frame MC, Calpain 2 and Src dependence distinguishes mesenchymal and amoeboid modes of tumour cell invasion: a link to integrin function. Oncogene. 2006 Sep 21;25(42):5726-40. Epub 2006 May 1.

[PubMed ID: 16816119] ... Su Y, Cui Z, Li Z, Block ER, Calpain-2 regulation of VEGF-mediated angiogenesis. FASEB J. 2006 Jul;20(9):1443-51.

[PubMed ID: 16598790] ... Courdier-Fruh I, Briguet A, Utrophin is a calpain substrate in muscle cells. Muscle Nerve. 2006 Jun;33(6):753-9.

[PubMed ID: 12665801] ... Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J, Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat Biotechnol. 2003 May;21(5):566-9. Epub 2003 Mar 31.

[PubMed ID: 1527057] ... Adachi Y, Kitahara-Ozawa A, Sugamura K, Lee WJ, Yodoi J, Maki M, Murachi T, Hatanaka M, Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I. J Biol Chem. 1992 Sep 25;267(27):19373-8.

[PubMed ID: 2209092] ... Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K, Four genes for the calpain family locate on four distinct human chromosomes. Cytogenet Cell Genet. 1990;53(4):225-9.

[PubMed ID: 2539381] ... Hata A, Ohno S, Akita Y, Suzuki K, Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease. J Biol Chem. 1989 Apr 15;264(11):6404-11.

[PubMed ID: 2852952] ... Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K, Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease. Biochemistry. 1988 Oct 18;27(21):8122-8.

[PubMed ID: 1067155] ... Kopp S, Reproducibility of response to a questionnaire on symptoms of masticatory dysfunction. Community Dent Oral Epidemiol. 1976 Sep;4(5):205-9.