PSSM Logo (SB)
Position Specific Scoring Matrix (SB)
Position | -10 | -9 | -8 | -7 | -6 | -5 | -4 | -3 | -2 | -1 | +1 | +2 | +3 | +4 | +5 | +6 | +7 | +8 | +9 | +10 | ||
A | Ala | -.01 | .09 | .19 | -.01 | .17 | .22 | .18 | -.00 | -.46 | -.26 | .23 | .15 | .02 | .12 | -.06 | .07 | .03 | .02 | -.02 | -.10 | |
C | Cys | .13 | -.27 | -.10 | -.27 | -.27 | -.58 | N/A | N/A | .20 | -.58 | -.58 | -.28 | -.28 | -.28 | -.27 | .03 | -.57 | -.57 | -.56 | -.55 | |
D | Asp | -.27 | -.12 | -.28 | -.17 | -.22 | -.13 | -.36 | -.29 | -.09 | -.23 | -.66 | -.43 | -.83 | -.13 | -.05 | -.08 | -.01 | -.42 | -.15 | -.26 | |
E | Glu | .20 | .06 | -.07 | -.02 | .03 | -.22 | -.03 | .20 | -.19 | -.03 | -.08 | .11 | -.33 | -.08 | .08 | -.08 | -.07 | .17 | .00 | .06 | |
F | Phe | -.03 | .05 | -.15 | .01 | .08 | .08 | -.70 | -.22 | -.53 | .11 | -.30 | -.05 | -.53 | -.05 | -.30 | -.39 | .01 | -.29 | -.68 | -.27 | |
G | Gly | -.09 | -.16 | -.28 | -.03 | -.06 | -.10 | -.17 | -.21 | -.25 | .07 | -.02 | -.07 | -.25 | .01 | .12 | -.06 | -.01 | .09 | .16 | .04 | |
H | His | .09 | -.06 | -.28 | .02 | -.06 | -.16 | .08 | -.16 | -.16 | .14 | -.47 | .14 | -.07 | .08 | .09 | -.16 | -.06 | -.05 | -.14 | -.04 | |
I | Ile | -.05 | -.16 | -.39 | -.32 | -.47 | -.39 | -.48 | -.10 | -.13 | -.87 | -.27 | .00 | -.33 | -.17 | -.39 | -.21 | -.39 | -.56 | -.11 | -.45 | |
K | Lys | .07 | -.13 | .19 | .25 | .17 | .10 | .18 | .22 | -.40 | .12 | .14 | -.07 | .28 | -.10 | .27 | .19 | .15 | .20 | .17 | .21 | |
L | Leu | -.20 | -.23 | -.38 | -.30 | -.38 | -.18 | -.19 | -.22 | .36 | -.11 | -.14 | -.16 | -.28 | -.31 | -.27 | -.15 | -.04 | -.17 | -.16 | -.22 | |
M | Met | -.17 | .01 | .00 | -.08 | -.30 | -.00 | -.09 | -.78 | -.18 | .06 | -.31 | -.09 | -.09 | -.78 | -.78 | -.78 | .00 | -.17 | -.46 | .15 | |
N | Asn | -.01 | -.16 | -.53 | -.01 | .03 | -.41 | .09 | -.18 | .12 | -.02 | -.07 | .06 | .21 | .10 | .22 | -.01 | -.01 | .08 | .28 | .12 | |
P | Pro | .07 | .12 | .17 | -.13 | .22 | -.09 | .23 | .25 | -.39 | -.49 | -.49 | .23 | .61 | .46 | .15 | .15 | -.00 | .18 | .11 | .30 | |
Q | Gln | .12 | .18 | .08 | .05 | .12 | .11 | .20 | .04 | .17 | .27 | .11 | .23 | .11 | -.10 | .04 | .15 | .08 | -.29 | .02 | -.20 | |
R | Arg | .08 | .21 | .40 | .25 | .07 | .26 | .18 | -.24 | -.03 | .27 | .29 | .06 | .27 | .07 | .17 | .21 | .12 | .27 | .19 | .12 | |
S | Ser | .11 | -.06 | .13 | .13 | .20 | .19 | .17 | .01 | .15 | -.04 | .30 | .08 | -.20 | .02 | -.03 | .02 | .21 | .03 | .08 | .07 | |
T | Thr | -.17 | .13 | -.09 | .05 | -.02 | -.02 | .18 | .07 | .20 | .37 | .18 | .14 | -.29 | .07 | -.02 | -.23 | -.05 | -.04 | -.27 | .21 | |
V | Val | .08 | .08 | -.05 | .10 | -.23 | .13 | -.33 | .26 | .18 | -.39 | -.19 | -.28 | -.09 | -.00 | -.08 | .03 | -.05 | -.07 | .05 | -.12 | |
W | Trp | N/A | .03 | .15 | -.45 | -.15 | -.15 | .24 | .39 | N/A | .02 | .02 | -.46 | .32 | .24 | .02 | .03 | -.45 | .34 | -.43 | -.13 | |
Y | Tyr | -.09 | .03 | .17 | -.03 | .08 | -.28 | -.58 | -.04 | -.19 | .19 | -.11 | N/A | -.11 | -.28 | -.10 | .30 | -.18 | -.02 | -.08 | -.08 |
Frequency Counts + Two-Sided Binomial Exact Test P-Values (SB)
Position | -10 | -9 | -8 | -7 | -6 | -5 | -4 | -3 | -2 | -1 | +1 | +2 | +3 | +4 | +5 | +6 | +7 | +8 | +9 | +10 | ||
Count | 246 | 248 | 249 | 249 | 250 | 252 | 255 | 255 | 255 | 255 | 255 | 255 | 255 | 253 | 251 | 250 | 249 | 245 | 240 | 238 | ||
A | Ala | 19 | 24 | 30 | 19 | 29 | 33 | 30 | 20 | 7 | 11 | 34 | 28 | 21 | 26 | 17 | 23 | 21 | 20 | 18 | 15 | |
1.00 | 0.29 | 0.02 | 1.00 | 0.03 | 0.00 | 0.03 | 1.00 | 0.00 | 0.04 | 0.00 | 0.08 | 0.82 | 0.16 | 0.64 | 0.41 | 0.72 | 0.81 | 1.00 | 0.47 | |||
C | Cys | 5 | 2 | 3 | 2 | 2 | 1 | 0 | 0 | 6 | 1 | 1 | 2 | 2 | 2 | 2 | 4 | 1 | 1 | 1 | 1 | |
0.43 | 0.60 | 1.00 | 0.60 | 0.60 | 0.19 | 0.04 | 0.04 | 0.29 | 0.20 | 0.20 | 0.60 | 0.60 | 0.60 | 0.60 | 0.79 | 0.19 | 0.28 | 0.28 | 0.28 | |||
D | Asp | 7 | 10 | 7 | 9 | 8 | 10 | 6 | 7 | 11 | 8 | 3 | 5 | 2 | 10 | 12 | 11 | 13 | 5 | 9 | 7 | |
0.09 | 0.48 | 0.09 | 0.26 | 0.16 | 0.40 | 0.03 | 0.07 | 0.58 | 0.16 | 0.00 | 0.01 | 0.00 | 0.40 | 0.89 | 0.67 | 1.00 | 0.02 | 0.39 | 0.11 | |||
E | Glu | 26 | 19 | 14 | 16 | 18 | 10 | 16 | 27 | 11 | 16 | 14 | 22 | 8 | 14 | 20 | 14 | 14 | 24 | 16 | 18 | |
0.02 | 0.52 | 0.61 | 1.00 | 0.70 | 0.10 | 0.90 | 0.02 | 0.17 | 0.90 | 0.53 | 0.21 | 0.02 | 0.61 | 0.38 | 0.61 | 0.61 | 0.05 | 1.00 | 0.52 | |||
F | Phe | 9 | 11 | 7 | 10 | 12 | 12 | 2 | 6 | 3 | 13 | 5 | 9 | 3 | 9 | 5 | 4 | 10 | 5 | 2 | 5 | |
1.00 | 0.62 | 0.51 | 0.87 | 0.51 | 0.51 | 0.01 | 0.26 | 0.02 | 0.33 | 0.11 | 0.87 | 0.02 | 0.87 | 0.14 | 0.05 | 0.87 | 0.14 | 0.01 | 0.18 | |||
G | Gly | 14 | 12 | 9 | 16 | 15 | 14 | 12 | 11 | 10 | 21 | 17 | 15 | 10 | 18 | 23 | 15 | 17 | 21 | 24 | 18 | |
0.53 | 0.21 | 0.04 | 0.90 | 0.71 | 0.46 | 0.18 | 0.11 | 0.06 | 0.39 | 1.00 | 0.62 | 0.06 | 0.90 | 0.17 | 0.71 | 1.00 | 0.31 | 0.07 | 0.70 | |||
H | His | 7 | 5 | 3 | 6 | 5 | 4 | 7 | 4 | 4 | 8 | 2 | 8 | 5 | 7 | 7 | 4 | 5 | 5 | 4 | 5 | |
0.52 | 1.00 | 0.39 | 0.83 | 1.00 | 0.67 | 0.53 | 0.67 | 0.67 | 0.40 | 0.14 | 0.40 | 1.00 | 0.53 | 0.52 | 0.67 | 1.00 | 1.00 | 0.67 | 1.00 | |||
I | Ile | 13 | 10 | 6 | 7 | 5 | 6 | 5 | 12 | 11 | 2 | 8 | 15 | 7 | 10 | 6 | 9 | 6 | 4 | 11 | 5 | |
0.79 | 0.28 | 0.01 | 0.04 | 0.01 | 0.02 | 0.00 | 0.51 | 0.35 | 0.00 | 0.06 | 1.00 | 0.03 | 0.23 | 0.01 | 0.14 | 0.01 | 0.00 | 0.49 | 0.01 | |||
K | Lys | 17 | 11 | 23 | 26 | 22 | 19 | 23 | 25 | 6 | 20 | 21 | 13 | 29 | 12 | 28 | 23 | 21 | 23 | 21 | 23 | |
0.50 | 0.42 | 0.04 | 0.01 | 0.06 | 0.28 | 0.05 | 0.02 | 0.01 | 0.19 | 0.14 | 0.69 | 0.00 | 0.51 | 0.00 | 0.04 | 0.11 | 0.03 | 0.07 | 0.02 | |||
L | Leu | 15 | 14 | 10 | 12 | 10 | 16 | 16 | 15 | 57 | 19 | 18 | 17 | 13 | 12 | 13 | 17 | 22 | 16 | 16 | 14 | |
0.07 | 0.03 | 0.00 | 0.01 | 0.00 | 0.09 | 0.07 | 0.04 | 0.00 | 0.29 | 0.20 | 0.11 | 0.01 | 0.01 | 0.01 | 0.13 | 0.75 | 0.10 | 0.13 | 0.05 | |||
M | Met | 4 | 6 | 6 | 5 | 3 | 6 | 5 | 1 | 4 | 7 | 3 | 5 | 5 | 1 | 1 | 1 | 6 | 4 | 2 | 8 | |
0.67 | 0.84 | 0.84 | 1.00 | 0.30 | 1.00 | 0.84 | 0.04 | 0.54 | 0.68 | 0.30 | 0.84 | 0.84 | 0.04 | 0.04 | 0.04 | 0.84 | 0.67 | 0.14 | 0.29 | |||
N | Asn | 10 | 7 | 3 | 10 | 11 | 4 | 13 | 7 | 14 | 10 | 9 | 12 | 17 | 13 | 17 | 10 | 10 | 12 | 19 | 13 | |
1.00 | 0.42 | 0.02 | 1.00 | 0.75 | 0.04 | 0.43 | 0.34 | 0.27 | 1.00 | 0.75 | 0.63 | 0.06 | 0.43 | 0.05 | 1.00 | 1.00 | 0.52 | 0.01 | 0.32 | |||
P | Pro | 14 | 16 | 18 | 9 | 20 | 10 | 21 | 22 | 5 | 4 | 4 | 21 | 50 | 35 | 17 | 17 | 12 | 18 | 15 | 23 | |
0.55 | 0.23 | 0.10 | 0.46 | 0.03 | 0.66 | 0.02 | 0.01 | 0.03 | 0.01 | 0.01 | 0.02 | 0.00 | 0.00 | 0.18 | 0.14 | 1.00 | 0.07 | 0.29 | 0.00 | |||
Q | Gln | 13 | 15 | 12 | 11 | 13 | 13 | 16 | 11 | 15 | 19 | 13 | 17 | 13 | 8 | 11 | 14 | 12 | 5 | 10 | 6 | |
0.32 | 0.10 | 0.51 | 0.63 | 0.33 | 0.33 | 0.07 | 0.75 | 0.14 | 0.01 | 0.33 | 0.04 | 0.33 | 0.63 | 0.74 | 0.19 | 0.51 | 0.14 | 0.87 | 0.32 | |||
R | Arg | 16 | 22 | 34 | 24 | 16 | 25 | 21 | 8 | 13 | 26 | 27 | 16 | 26 | 16 | 20 | 22 | 18 | 25 | 20 | 17 | |
0.48 | 0.02 | 0.00 | 0.01 | 0.48 | 0.00 | 0.05 | 0.13 | 1.00 | 0.00 | 0.00 | 0.49 | 0.00 | 0.49 | 0.09 | 0.02 | 0.21 | 0.00 | 0.06 | 0.25 | |||
S | Ser | 22 | 15 | 23 | 23 | 27 | 27 | 26 | 18 | 25 | 16 | 35 | 21 | 11 | 18 | 16 | 18 | 28 | 18 | 20 | 19 | |
0.21 | 0.71 | 0.13 | 0.13 | 0.02 | 0.02 | 0.05 | 0.90 | 0.08 | 0.80 | 0.00 | 0.38 | 0.14 | 0.80 | 0.90 | 0.80 | 0.01 | 0.71 | 0.37 | 0.52 | |||
T | Thr | 9 | 18 | 11 | 15 | 13 | 13 | 21 | 16 | 22 | 32 | 21 | 19 | 7 | 16 | 13 | 8 | 12 | 12 | 7 | 21 | |
0.26 | 0.20 | 0.58 | 0.67 | 1.00 | 1.00 | 0.05 | 0.49 | 0.04 | 0.00 | 0.05 | 0.16 | 0.07 | 0.49 | 1.00 | 0.16 | 0.78 | 0.89 | 0.11 | 0.03 | |||
V | Val | 20 | 20 | 15 | 21 | 10 | 23 | 8 | 31 | 26 | 7 | 11 | 9 | 14 | 17 | 14 | 18 | 15 | 14 | 18 | 12 | |
0.37 | 0.37 | 0.80 | 0.26 | 0.10 | 0.13 | 0.02 | 0.00 | 0.03 | 0.01 | 0.13 | 0.04 | 0.53 | 1.00 | 0.61 | 0.71 | 0.80 | 0.61 | 0.61 | 0.36 | |||
W | Trp | 0 | 3 | 4 | 1 | 2 | 2 | 5 | 7 | 0 | 3 | 3 | 1 | 6 | 5 | 3 | 3 | 1 | 6 | 1 | 2 | |
0.12 | 0.76 | 0.37 | 0.54 | 1.00 | 1.00 | 0.22 | 0.03 | 0.13 | 0.77 | 0.77 | 0.38 | 0.07 | 0.22 | 0.76 | 0.76 | 0.54 | 0.06 | 0.53 | 1.00 | |||
Y | Tyr | 6 | 8 | 11 | 7 | 9 | 4 | 2 | 7 | 5 | 12 | 6 | 0 | 6 | 4 | 6 | 15 | 5 | 7 | 6 | 6 | |
0.85 | 0.85 | 0.19 | 1.00 | 0.57 | 0.26 | 0.04 | 1.00 | 0.46 | 0.14 | 0.71 | 0.00 | 0.71 | 0.26 | 0.71 | 0.01 | 0.46 | 1.00 | 0.85 | 0.85 |
The procedure for computing each score
The above score of an amino acid at each position was computed by first dividing the occururence ratio of an amino acid to all amino acids by the composition ratio of this amino acid to all amino acids and then taking the logarithm of this.
We used the following amino acid composition ratio from UniProtKB/Swiss-Prot protein knowledgebase release 53.3 statistics:
Ala (A) 7.86 Gln (Q) 3.97 Leu (L) 9.66 Ser (S) 6.87 Arg (R) 5.43 Glu (E) 6.66 Lys (K) 5.93 Thr (T) 5.40 Asn (N) 4.12 Gly (G) 6.94 Met (M) 2.39 Trp (W) 1.13 Asp (D) 5.33 His (H) 2.29 Phe (F) 3.95 Tyr (Y) 3.01 Cys (C) 1.50 Ile (I) 5.88 Pro (P) 4.84 Val (V) 6.72