SB0004 : Calcium/calmodulin-dependent 3p,5p-cyclic nucleotide phosphodiesterase1A
[ CaMP Format ]
* Basic Information
| Organism | Bos taurus (cattle) |
| Protein Names | 61 kDa calmodulin-stimulated cyclic nucleotide phosphodiesterase [Bos taurus]; 61 kDa calmodulin-stimulated cyclic nucleotide phosphodiesterase; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; Cam-PDE 1A; 3.1.4.17; 61 kDa Cam-PDE |
| Gene Names | CNP; PDE1A |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 530 aa
Average Mass: 60.843 kDa
Monoisotopic Mass: 60.805 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| 3'5'-cyclic nucleotide phosphodiesterase N-terminal 1. | 73 | 133 | 1.0 | 0.2 |
| --- cleavage 126 (inside 3'5'-cyclic nucleotide phosphodiesterase N-terminal 73..133) --- |
| 3'5'-cyclic nucleotide phosphodiesterase 1. | 218 | 438 | 1.0 | 5.8 |
3. Sequence Information
Fasta Sequence: SB0004.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
1 [sites]
Source Reference: [PubMed ID: 9784246] Kakkar R, Raju RV, Sharma RK, In vitro generation of an active calmodulin-independent phosphodiesterase from brain calmodulin-dependent phosphodiesterase (PDE1A2) by m-calpain. Arch Biochem Biophys. 1998 Oct 15;358(2):320-8.
Cleavage sites (±10aa)
[Site 1] RFRSIVHVVQ126-AGIFVERMYR
Gln126 
Ala
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Arg117 | Phe118 | Arg119 | Ser120 | Ile121 | Val122 | His123 | Val124 | Val125 | Gln126 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala127 | Gly128 | Ile129 | Phe130 | Val131 | Glu132 | Arg133 | Met134 | Tyr135 | Arg136 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
LASTFTRKMGMMKKKSEEKPRFRSIVHVVQAGIFVERMYRKSYHMVGLAYPEAVIVTLKD
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
126.00 |
4 |
PDE1A_BOVIN Calcium/calmodulin-dependent 3',5'-cy |
| 2 |
Bos taurus |
126.00 |
2 |
phosphodiesterase 1A, calmodulin-dependent |
| 3 |
Canis lupus familiaris |
121.00 |
1 |
phosphodiesterase 1A, calmodulin-dependent |
| 4 |
Canis familiaris |
101.00 |
2 |
PREDICTED: similar to phosphodiesterase 1C, calmo |
| 5 |
Homo sapiens |
100.00 |
14 |
PDE1C protein |
| 6 |
Mus musculus |
100.00 |
12 |
unnamed protein product |
| 7 |
Rattus norvegicus |
100.00 |
3 |
phosphodiesterase 1C |
| 8 |
Mus musculus |
100.00 |
1 |
phosphodiesterase 1C isoform a |
| 9 |
Pongo pygmaeus |
98.60 |
3 |
hypothetical protein |
| 10 |
Tetraodon nigroviridis |
93.60 |
5 |
unnamed protein product |
| 11 |
Xenopus laevis |
75.50 |
1 |
hypothetical protein LOC495332 |
| 12 |
Macaca fascicularis |
73.90 |
1 |
hypothetical protein |
| 13 |
Drosophila melanogaster |
72.00 |
2 |
RE69157p |
| 14 |
Drosophila pseudoobscura |
67.80 |
1 |
GA13368-PA |
| 15 |
Caenorhabditis briggsae |
42.00 |
1 |
Hypothetical protein CBG20362 |
| 16 |
Caenorhabditis elegans |
42.00 |
1 |
PhosphoDiEsterase family member (pde-1) |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|
| Bos taurus |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|
| Canis lupus familiaris |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
||||||||||||||||||||+|||||| ||#|||||||||||||||||| |||||||||||
Sbjct 81 LASTFTRKMGMMKKKSEEKPKFRSIVHAVQ#AGIFVERMYRKSYHMVGLVYPEAVIVTLKD 140
|
| Canis familiaris |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+|||| ++|||||||+|||| ||#||||||||||++ +||||+|| ||| |||
Sbjct 67 LASTFTRQMGMMLRRSEEKPRFKSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPAVIEALKD 126
|
| Homo sapiens |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+|||| ++|+|||||+|||| ||#||||||||||++ +||||+|| ||| |||
Sbjct 106 LASTFTRQMGMMLRRSDEKPRFKSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPAVIEALKD 165
|
| Mus musculus |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+|||| ++|+|||||+|||| ||#||||||||||++ +||||+|| ||| |||
Sbjct 78 LASTFTRQMGMMLRRSDEKPRFKSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPAVIDALKD 137
|
| Rattus norvegicus |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+|||| ++|+|||||+|||| ||#||||||||||++ +||||+|| ||| |||
Sbjct 166 LASTFTRQMGMMLRRSDEKPRFKSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPAVIDALKD 225
|
| Mus musculus |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+|||| ++|+|||||+|||| ||#||||||||||++ +||||+|| ||| |||
Sbjct 106 LASTFTRQMGMMLRRSDEKPRFKSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPAVIDALKD 165
|
| Pongo pygmaeus |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+|||| ++|||||||+|||| ||#||||||||||++ +||||+|| ||| |||
Sbjct 106 LASTFTRQMGMMLRRSEEKPRFKSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPAVIEALKD 165
|
| Tetraodon nigroviridis |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+||+| +++||||||||||| ||#||||||||||++ +||||+|| +|| ||+
Sbjct 163 LASTFTRQMGLMLRRNEEKPRFRSIVHAVQ#AGIFVERMYRRTSNMVGLSYPPSVISVLKN 222
|
| Xenopus laevis |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLK 155
||||||++ ++|||||||||||| ||#||||||||+|+|| || | |+ +||
Sbjct 98 LASTFTQQTRSTGRRSEEKPRFRSIVHAVQ#AGIFVERMFRRSYTAVGPNYSADVVNSLK 156
|
| Macaca fascicularis |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
||||||++ +++||||+|||||| ||#||||||||+|++| || | ||+ |+
Sbjct 101 LASTFTQQARAKGRRAEEKPKFRSIVHAVQ#AGIFVERMFRRTYTSVGPTYSTAVLNCFKN 160
|
| Drosophila melanogaster |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+| ++||+|||+|||+ | ++#|||||+||||+ |+| |+ ||+
Sbjct 347 LASTFTRQMATSRRKSDEKPKFRSVAHAIR#AGIFVDRMYRRVSSSALTAFPPDVVRLLKN 406
|
| Drosophila pseudoobscura |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRKSYHMVGLAYPEAVIVTLKD 156
|||||||+| ++||+|||+|||+ | ++#|||||+|+||+ + +| |+ ||+
Sbjct 86 LASTFTRQMATSRRKSDEKPKFRSVAHAIR#AGIFVDRIYRRVSSSALMQFPPDVVKLLKN 145
|
| Caenorhabditis briggsae |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRK 137
||+||||+ | +||+|+|+ + ++# ||| |+++||
Sbjct 215 LAATFTRQNA---GKKRDKPKFKSVANAIR#TGIFFEKLFRK 252
|
| Caenorhabditis elegans |
Query 97 LASTFTRKMGMMKKKSEEKPRFRSIVHVVQ#AGIFVERMYRK 137
||+||||+ | +||+|+|+ + ++# ||| |+++||
Sbjct 215 LAATFTRQNA---GKKRDKPKFKSVANAIR#TGIFFEKLFRK 252
|
* References
[PubMed ID: 7678006] Sonnenburg WK, Seger D, Beavo JA, Molecular cloning of a cDNA encoding the "61-kDa" calmodulin-stimulated cyclic nucleotide phosphodiesterase. Tissue-specific expression of structurally related isoforms. J Biol Chem. 1993 Jan 5;268(1):645-52.
