CaMPDB :: Substrate

SB0007 : Glutamate receptor, metabotropic 1

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	metabotropic glutamate receptor 1 isoform alpha precursor [Rattus norvegicus]; metabotropic glutamate receptor 1 isoform alpha precursor; metabotropic glutamate receptor 1; mGluR1; G protein coupled receptor family C group 1 member A; Metabotropic glutamate receptor 1
Gene Names	Grm1; Gprc1a, Mglur1; Gprc1a; Mglur1; glutamate receptor, metabotropic 1
Gene Locus	1p13; chromosome 1
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_058707	NM_017011	24414	P23385	N/A	N/A	N/A	rno:24414

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 1199 aa

Average Mass: 133.235 kDa

Monoisotopic Mass: 133.151 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
P-loop containing NTP hydrolase pore-1 1.	270	311	195.0	0.0
Nine Cysteines Domain of family 3 GPCR 1.	521	571	2.0	10.4
7 transmembrane sweet-taste receptor of 3 GCPR 1.	604	837	1.0	27.4
--- cleavage 936 ---
Homer-binding domain of metabotropic glutamate receptor 1.	1149	1199	1.0	9.5

3. Sequence Information

Fasta Sequence: SB0007.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1EWK (X-ray; 220 A; A/B=33-522), 1EWT (X-ray; 370 A; A/B=33-522), 1EWV (X-ray; 400 A; A/B=33-522), 1ISR (X-ray; 400 A; A=33-522), 1ISS (X-ray; 330 A; A/B=33-522)

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 17270736] Xu W, Wong TP, Chery N, Gaertner T, Wang YT, Baudry M, Calpain-mediated mGluR1alpha truncation: a key step in excitotoxicity. Neuron. 2007 Feb 1;53(3):399-412.

Cleavage sites (±10aa)

[Site 1] TAVIKPLTKS⁹³⁶-YQGSGKSLTF

Ser⁹³⁶ Tyr

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Thr⁹²⁷	Ala⁹²⁸	Val⁹²⁹	Ile⁹³⁰	Lys⁹³¹	Pro⁹³²	Leu⁹³³	Thr⁹³⁴	Lys⁹³⁵	Ser⁹³⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Tyr⁹³⁷	Gln⁹³⁸	Gly⁹³⁹	Ser⁹⁴⁰	Gly⁹⁴¹	Lys⁹⁴²	Ser⁹⁴³	Leu⁹⁴⁴	Thr⁹⁴⁵	Phe⁹⁴⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
QHVWQRLSVHVKTNETACNQTAVIKPLTKSYQGSGKSLTFSDASTKTLYNVEEEDNTPSA

Summary

#	organism	max score	hits	top seq
1	Rattus norvegicus	127.00	4	glutamate receptor, metabotropic 1 isoform alpha
2	Mus musculus	125.00	4	glutamate receptor, metabotropic 1 isoform alpha
3	Canis familiaris	114.00	3	PREDICTED: similar to glutamate receptor, metabot
4	Homo sapiens	111.00	4	glutamate receptor, metabotropic 1 isoform alpha
5	N/A	111.00	2	MGR1_HUMAN Metabotropic glutamate receptor 1 prec
6	Oncorhynchus masou	75.10	1	bifunctional metabotropic receptor for extracellu
7	Tetraodon nigroviridis	70.90	3	unnamed protein product
8	Macaca mulatta	68.90	2	metabotropic glutamate receptor 1
9	Gallus gallus	50.10	3	glutamate receptor, metabotropic 5
10	Canis familiaris	47.40	2	PREDICTED: similar to Metabotropic glutamate rece
11	Streptococcus agalactiae NEM316	33.90	1	hypothetical protein gbs0384

Top-ranked sequences

organism	matching
Rattus norvegicus	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDNTPSA 966 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDNTPSA 966
Mus musculus	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDNTPS 965 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDNTPS 965
Canis familiaris	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDNTP 964 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \| Sbjct 907 QHMWHRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDTSTKTLYNVEEEDAQP 964
Homo sapiens	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDN 962 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|++ Sbjct 907 QHMWHRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDTSTKTLYNVEEEED 962
N/A	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDN 962 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|++ Sbjct 907 QHMWHRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDTSTKTLYNVEEEED 962
Oncorhynchus masou	Query 907 QHVWQRLSVHVKTNETAC-NQTAVIKPLTKS#YQGSGKSLT--FSDASTKTLYNVEEEDNT 963 +\| \|\|\|\|\|\|\|\|\| \| \| \|\| \|\|\|+\|\|\| +#\| +\| \|+ \|\|\| \|\|\|\|\|\|\|\| \|\|\| + Sbjct 915 EHNWQRLSVHVKRQEAGCSNQMAVIRPLTNT#YHNTGSSMAMEFSDLSTKTLYNVAEEDES 974
Tetraodon nigroviridis	Query 908 HVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDNTPS 965 \|+\| \|\|\|\|\|\|\| \|+ \|\| \|\|\|\|\|\|\| +#\|\| \| \|\|+ \|\|\|\|\|\|\|\| \|\|\| + + Sbjct 978 HMWHRLSVHVKRQESGSNQMAVIKPLTNT#YQ---NRLEFSNLSTKTLYNVAEEDESET 1032
Macaca mulatta	Query 928 AVIKPLTKS#YQGSGKSLTFSDASTKTLYNVEEEDN 962 \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|++ Sbjct 1 AVIKPLTKS#YQGSGKSLTFSDTSTKTLYNVEEEED 35
Gallus gallus	Query 908 HVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSL--TFSDASTKTLYNVEE-EDNTP 964 \|+\|\|\|\|\|+\|+ \| \|\|\|\|\|\|\|\| +\|\|# \| \| \|\| + \| \|\|\|\|+\| \| \|+ \| Sbjct 988 HLWQRLSIHINKKENP-NQTAVIKPFSKS#TDSSRHSSSATFPETSAKTLYDVSEAEEQYP 1046 Query 965 S 965 + Sbjct 1047 A 1047
Canis familiaris	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSG--KSLTFSDASTKTLYNV-EEEDNT 963 \|\|+\|\|\|\|\|+\|+ \|+ \|\|\|\|\|\|\|\| \|\|# + \| \|\| \| \|\|+\| \| \|++ Sbjct 927 QHLWQRLSIHINKKESP-NQTAVIKPFPKS#PESRGLGAGGGPPDAGPKALYDVAEAEEHF 985 Query 964 PS 965 \|+ Sbjct 986 PA 987
Streptococcus agalactiae NEM316	Query 907 QHVWQRLSVHVKTNETACNQTAVIKPLTKS#YQGSGKSLTFSDASTKTLYNV 957 \| \|\|+\| \|\| \| +\|+\|+ \|\|#\| \|\| + + + \|+\|++ Sbjct 430 QKAWQKLGYFVKAGEQPLE---IIRPVYKS#YDKHGKGIGRPEFVSDTVYDI 477

* References

[PubMed ID: 23978512] Fan W, Group I metabotropic glutamate receptors modulate late phase long-term potentiation in hippocampal CA1 pyramidal neurons: comparison of apical and basal dendrites. Neurosci Lett. 2013 Oct 11;553:132-7. doi: 10.1016/j.neulet.2013.08.030. Epub

» More (9) « Hide Below

[PubMed ID: 22362014] ... Wang XM, Liu WJ, Zhang R, Zhou YK, Effects of exposure to low-level lead on spatial learning and memory and the expression of mGluR1, NMDA receptor in different developmental stages of rats. Toxicol Ind Health. 2013 Sep;29(8):686-96. doi: 10.1177/0748233712436641. Epub

[PubMed ID: 23426668] ... Jin DZ, Guo ML, Xue B, Fibuch EE, Choe ES, Mao LM, Wang JQ, Phosphorylation and feedback regulation of metabotropic glutamate receptor 1 by calcium/calmodulin-dependent protein kinase II. J Neurosci. 2013 Feb 20;33(8):3402-12. doi: 10.1523/JNEUROSCI.3192-12.2013.

[PubMed ID: 22973005] ... Govindaiah G, Wang T, Gillette MU, Cox CL, Activity-dependent regulation of retinogeniculate signaling by metabotropic glutamate receptors. J Neurosci. 2012 Sep 12;32(37):12820-31. doi: 10.1523/JNEUROSCI.0687-12.2012.

[PubMed ID: 22442667] ... Sun L, Gu L, Wang S, Yuan J, Yang H, Zhu J, Zhang H, N-acetylcysteine protects against apoptosis through modulation of group I metabotropic glutamate receptor activity. PLoS One. 2012;7(3):e32503. doi: 10.1371/journal.pone.0032503. Epub 2012 Mar 19.

[PubMed ID: 8312606] ... O'Hara PJ, Gilbert TL, Haldeman BA, Houamed KM, Kuijper JL, Hagen FS, Almers W, Mulvihill E, Different sites of polyadenylation in mRNAs encoding a rat metabotropic glutamate receptor. DNA Seq. 1993;4(1):53-7.

[PubMed ID: 1438218] ... Pin JP, Waeber C, Prezeau L, Bockaert J, Heinemann SF, Alternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10331-5.

[PubMed ID: 1309649] ... Tanabe Y, Masu M, Ishii T, Shigemoto R, Nakanishi S, A family of metabotropic glutamate receptors. Neuron. 1992 Jan;8(1):169-79.

[PubMed ID: 1656524] ... Houamed KM, Kuijper JL, Gilbert TL, Haldeman BA, O'Hara PJ, Mulvihill ER, Almers W, Hagen FS, Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain. Science. 1991 May 31;252(5010):1318-21.

[PubMed ID: 1847995] ... Masu M, Tanabe Y, Tsuchida K, Shigemoto R, Nakanishi S, Sequence and expression of a metabotropic glutamate receptor. Nature. 1991 Feb 28;349(6312):760-5.