logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0009 : Neurofibromin 2, Merlin

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesmerlin isoform 1 [Homo sapiens]; merlin isoform 1; moesin-ezrin-radixin like; neurofibromin 2 (bilateral acoustic neuroma); schwannomin; moesin-ezrin-radizin-like protein; schwannomerlin; merlin; neurofibromin-2; moesin-ezrin-radixin-like protein; Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin
Gene NamesNF2; SCH; neurofibromin 2 (merlin)
Gene Locus22q12.2; chromosome 22
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_000259 NM_000268 4771 P35240 607379 HGNC:7773 06980 hsa:4771

* Information From OMIM

Description: Cell-cell contact between normal cultured diploid cells results in inhibition of proliferation despite the unlimited availability of nutrients and growth-promoting factors. A similar phenomenon occurs in vivo in all adult solid tissues. NF2 is a critical regulator of contact-dependent inhibition of proliferation and functions at the interface between cell-cell adhesion, transmembrane signaling, and the actin cytoskeleton (Curto and McClatchey, 2008).

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* Structure Information

1. Primary Information

Length: 595 aa

Average Mass: 69.689 kDa

Monoisotopic Mass: 69.646 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
FERM C-terminal PH-like domain 1. 205043.00.0
FERM C-terminal PH-like domain 2. 11212478.00.1
FERM C-terminal PH-like domain 3. 2263131.00.0
--- cleavage 294 (inside FERM C-terminal PH-like domain 226..313) ---
--- cleavage 298 (inside FERM C-terminal PH-like domain 226..313) ---
FERM central domain 1. 32836167.01.2
FERM central domain 2. 38147820.017.5
FERM N-terminal domain 1. 51954043.00.2

3. Sequence Information

Fasta Sequence: SB0009.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1H4R (X-ray; 180 A; A/B=1-313), 3U8Z (X-ray; 264 A; A/B/C/D=18-312)

* Cleavage Information

2 [sites] cleaved by Calpain 1 and 2

Source Reference: [PubMed ID: 9701243] Kimura Y, Koga H, Araki N, Mugita N, Fujita N, Takeshima H, Nishi T, Yamashima T, Saido TC, Yamasaki T, Moritake K, Saya H, Nakao M, The involvement of calpain-dependent proteolysis of the tumor suppressor NF2 (merlin) in schwannomas and meningiomas. Nat Med. 1998 Aug;4(8):915-22.

Cleavage sites (±10aa)

[Site 1] FNSSKLRVNK294-LILQLCIGNH

Lys294 Leu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Phe285Asn286Ser287Ser288Lys289Leu290Arg291Val292Asn293Lys294
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Leu295Ile296Leu297Gln298Leu299Cys300Ile301Gly302Asn303His304

Sequence conservation (by blast)

[Site 2] KLRVNKLILQ298-LCIGNHDLFM

Gln298 Leu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Lys289Leu290Arg291Val292Asn293Lys294Leu295Ile296Leu297Gln298
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Leu299Cys300Ile301Gly302Asn303His304Asp305Leu306Phe307Met308

Sequence conservation (by blast)

* References

[PubMed ID: 24309211] Schulz A, Kyselyova A, Baader SL, Jung MJ, Zoch A, Mautner VF, Hagel C, Morrison H, Neuronal merlin influences ERBB2 receptor expression on Schwann cells through neuregulin 1 type III signalling. Brain. 2014 Feb;137(Pt 2):420-32. doi: 10.1093/brain/awt327. Epub 2013 Dec 5.

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