CaMPDB :: Substrate

SB0013 : Solute carrier family 6, Gly transporter 2

* Basic Information

Organism	Rattus norvegicus (Norway rat)
Protein Names	sodium- and chloride-dependent glycine transporter 2 [Rattus norvegicus]; sodium- and chloride-dependent glycine transporter 2; glycine transporter 2; glyT-2; solute carrier family 6 (neurotransmitter transporter, glycine), member 5; solute carrier family 6 member 5; Sodium- and chloride-dependent glycine transporter 2; GlyT-2; GlyT2; Solute carrier family 6 member 5
Gene Names	Slc6a5; Glyt2; solute carrier family 6 (neurotransmitter transporter), member 5
Gene Locus	1q22; chromosome 1
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_976079	NM_203334	171148	P58295	N/A	N/A	N/A	rno:171148

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 799 aa

Average Mass: 87.908 kDa

Monoisotopic Mass: 87.851 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 156 ---
--- cleavage 164 ---
Protein of unknown function (DUF999) 1.	345	398	34.0	0.0
Protein of unknown function (DUF999) 2.	491	540	64.0	0.1

3. Sequence Information

Fasta Sequence: SB0013.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites] cleaved by Calpain 1 and/or 2

Source Reference: [PubMed ID: 14675166] Baliova M, Betz H, Jursky F, Calpain-mediated proteolytic cleavage of the neuronal glycine transporter, GlyT2. J Neurochem. 2004 Jan;88(1):227-32.

Cleavage sites (±10aa)

[Site 1] NTPPVGWVNM¹⁵⁶-SQSTVVLGTD

Met¹⁵⁶ Ser

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asn¹⁴⁷	Thr¹⁴⁸	Pro¹⁴⁹	Pro¹⁵⁰	Val¹⁵¹	Gly¹⁵²	Trp¹⁵³	Val¹⁵⁴	Asn¹⁵⁵	Met¹⁵⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ser¹⁵⁷	Gln¹⁵⁸	Ser¹⁵⁹	Thr¹⁶⁰	Val¹⁶¹	Val¹⁶²	Leu¹⁶³	Gly¹⁶⁴	Thr¹⁶⁵	Asp¹⁶⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
LRGPEEDENVSVAKGTLEHNNTPPVGWVNMSQSTVVLGTDGIASVLPGSVATTTIPEDEQ

Summary

#	organism	max score	hits	top seq
1	Rattus norvegicus	126.00	2	solute carrier family 6 (neurotransmitter transpo
2	Mus musculus	119.00	3	SC6A5_MOUSE Sodium- and chloride-dependent glycin
3	N/A	103.00	1	SC6A5_HUMAN Sodium- and chloride-dependent glycin
4	Homo sapiens	101.00	2	AF352733_1 glycine type 2 transporter variant SC6
5	Bos taurus	99.40	1	PREDICTED: similar to glycine transporter GLYT2 i
6	Canis familiaris	89.70	1	PREDICTED: similar to Sodium- and chloride-depend
7	Tetraodon nigroviridis	47.80	1	unnamed protein product
8	Danio rerio	46.60	1	solute carrier family 6 (neurotransmitter transpo

Top-ranked sequences

organism	matching
Rattus norvegicus	Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186
Mus musculus	Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 \|\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 127 LRGPEEDANVSVGKGTLEHNNTPAVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186
N/A	Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 \|\|\|\|\| \| \|\|\|\| \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 125 LRGPEGDANVSVGKGTLERNNTPVVGWVNM#SQSTVVLGTDGITSVLPGSVATVATQEDEQ 184
Homo sapiens	Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 \|\|\|\|\| \| \|\|\|\| \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|+ Sbjct 125 LRGPEGDANVSVGKGTLERNNTPVVGWVNM#SQSTVVLGTDGITSVLPGSVATVATQEDER 184
Bos taurus	Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 \|\|\| \| \| \|\|\|\| \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 126 LRGLEGDANVSVGKGTLERNNTPAVGWVNM#SQSTVVLGTDGITSVLPGSVATAATQEDEQ 185
Canis familiaris	Query 127 LRGPEEDENVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 \|\|\|\|\| \| \|\| \|\|\|\|\| \|+\|\| \|\|\|\|\|#\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|+ \|\|\|\| Sbjct 232 LRGPEGDAAASVGKGTLERNSTPAGGWVNM#SQSAVVLGTDGITSVLPGSVASAAAQEDEQ 291
Tetraodon nigroviridis	Query 140 KGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDEQ 186 + \| \| \|\|\|\| \| \|#\|\|+\|++\|\|\|\|\| \|\| \|\|+\| \| +\|\|+ Sbjct 54 RATAEQNNTPGT-WTAM#SQTTIILGTDGNTSVQPGTV--TANDDDEE 97
Danio rerio	Query 135 NVSVAKGTLEHNNTPPVGWVNM#SQSTVVLGTDGIASVLPGSVATTTIPEDE 185 \| \| + \|\|\|\|+ \| +\|#\|\|+\|++\|\|\|\|\| \|\|\|\|\|+\| \| \| \|+ Sbjct 110 NQSAVRIAAEHNNSTG-DWTSM#SQTTIILGTDGNTSVLPGTV--TGPPTDD 157

[Site 2] NMSQSTVVLG¹⁶⁴-TDGIASVLPG

Gly¹⁶⁴ Thr

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Asn¹⁵⁵	Met¹⁵⁶	Ser¹⁵⁷	Gln¹⁵⁸	Ser¹⁵⁹	Thr¹⁶⁰	Val¹⁶¹	Val¹⁶²	Leu¹⁶³	Gly¹⁶⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Thr¹⁶⁵	Asp¹⁶⁶	Gly¹⁶⁷	Ile¹⁶⁸	Ala¹⁶⁹	Ser¹⁷⁰	Val¹⁷¹	Leu¹⁷²	Pro¹⁷³	Gly¹⁷⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
NVSVAKGTLEHNNTPPVGWVNMSQSTVVLGTDGIASVLPGSVATTTIPEDEQGDENKARG

Summary

#	organism	max score	hits	top seq
1	Rattus norvegicus	127.00	2	solute carrier family 6 (neurotransmitter transpo
2	Mus musculus	122.00	3	SC6A5_MOUSE Sodium- and chloride-dependent glycin
3	N/A	108.00	1	SC6A5_HUMAN Sodium- and chloride-dependent glycin
4	Bos taurus	108.00	1	PREDICTED: similar to glycine transporter GLYT2 i
5	Homo sapiens	107.00	2	AF352733_1 glycine type 2 transporter variant SC6
6	Canis familiaris	95.90	1	PREDICTED: similar to Sodium- and chloride-depend
7	Tetraodon nigroviridis	62.40	1	unnamed protein product
8	Danio rerio	52.00	1	solute carrier family 6 (neurotransmitter transpo

Top-ranked sequences

organism	matching
Rattus norvegicus	Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194
Mus musculus	Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 \|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 135 NVSVGKGTLEHNNTPAVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194
N/A	Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 \|\|\|\| \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 133 NVSVGKGTLERNNTPVVGWVNMSQSTVVLG#TDGITSVLPGSVATVATQEDEQGDENKARG 192
Bos taurus	Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 \|\|\|\| \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 134 NVSVGKGTLERNNTPAVGWVNMSQSTVVLG#TDGITSVLPGSVATAATQEDEQGDENKARG 193
Homo sapiens	Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 \|\|\|\| \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|\| \|\|\|+\|\|\|\|\|\|\|\| Sbjct 133 NVSVGKGTLERNNTPVVGWVNMSQSTVVLG#TDGITSVLPGSVATVATQEDERGDENKARG 192
Canis familiaris	Query 137 SVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 \|\| \|\|\|\|\| \|+\|\| \|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|+ \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 242 SVGKGTLERNSTPAGGWVNMSQSAVVLG#TDGITSVLPGSVASAAAQEDEQGDENKARG 299
Tetraodon nigroviridis	Query 140 KGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQGDENKARG 194 + \| \| \|\|\|\| \| \|\|\|+\|++\|\|#\|\|\| \|\| \|\|+\| \| ++\| \|\|\|\|\|\|\|\| Sbjct 54 RATAEQNNTPGT-WTAMSQTTIILG#TDGNTSVQPGTV-TANDDDEEGGDENKARG 106
Danio rerio	Query 135 NVSVAKGTLEHNNTPPVGWVNMSQSTVVLG#TDGIASVLPGSVATTTIPEDEQG------- 187 \| \| + \|\|\|\|+ \| +\|\|\|+\|++\|\|#\|\|\| \|\|\|\|\|+\| \| \| \|+ \| Sbjct 110 NQSAVRIAAEHNNSTG-DWTSMSQTTIILG#TDGNTSVLPGTV--TGPPTDDTGRLFQDDD 166 Query 188 -----DENKARG 194 \|\|\|\|\|\|\| Sbjct 167 DDDGGDENKARG 178

* References

[PubMed ID: 23650557] Arribas-Gonzalez E, Alonso-Torres P, Aragon C, Lopez-Corcuera B, Calnexin-assisted biogenesis of the neuronal glycine transporter 2 (GlyT2). PLoS One. 2013 May 1;8(5):e63230. doi: 10.1371/journal.pone.0063230. Print 2013.

» More (9) « Hide Below

[PubMed ID: 22132725] ... Perez-Siles G, Nunez E, Morreale A, Jimenez E, Leo-Macias A, Pita G, Cherubino F, Sangaletti R, Bossi E, Ortiz AR, Aragon C, Lopez-Corcuera B, An aspartate residue in the external vestibule of GLYT2 (glycine transporter 2) controls cation access and transport coupling. Biochem J. 2012 Mar 1;442(2):323-34. doi: 10.1042/BJ20110247.

[PubMed ID: 21910806] ... de Juan-Sanz J, Zafra F, Lopez-Corcuera B, Aragon C, Endocytosis of the neuronal glycine transporter GLYT2: role of membrane rafts and protein kinase C-dependent ubiquitination. Traffic. 2011 Dec;12(12):1850-67. doi: 10.1111/j.1600-0854.2011.01278.x. Epub

[PubMed ID: 21574997] ... Perez-Siles G, Morreale A, Leo-Macias A, Pita G, Ortiz AR, Aragon C, Lopez-Corcuera B, Molecular basis of the differential interaction with lithium of glycine transporters GLYT1 and GLYT2. J Neurochem. 2011 Jul;118(2):195-204. doi: 10.1111/j.1471-4159.2011.07309.x. Epub

[PubMed ID: 20335821] ... Nakamura M, Jang IS, GlyT-2 mediates the forskolin-induced increase of glycinergic transmission. Neuroreport. 2010 Apr 21;21(6):463-8. doi: 10.1097/WNR.0b013e3283390476.

[PubMed ID: 15010455] ... Fornes A, Nunez E, Aragon C, Lopez-Corcuera B, The second intracellular loop of the glycine transporter 2 contains crucial residues for glycine transport and phorbol ester-induced regulation. J Biol Chem. 2004 May 28;279(22):22934-43. Epub 2004 Mar 8.

[PubMed ID: 14675166] ... Baliova M, Betz H, Jursky F, Calpain-mediated proteolytic cleavage of the neuronal glycine transporter, GlyT2. J Neurochem. 2004 Jan;88(1):227-32.

[PubMed ID: 14512139] ... Tanaka I, Ezure K, Kondo M, Distribution of glycine transporter 2 mRNA-containing neurons in relation to glutamic acid decarboxylase mRNA-containing neurons in rat medulla. Neurosci Res. 2003 Oct;47(2):139-51.

[PubMed ID: 10722844] ... Geerlings A, Lopez-Corcuera B, Aragon C, Characterization of the interactions between the glycine transporters GLYT1 and GLYT2 and the SNARE protein syntaxin 1A. FEBS Lett. 2000 Mar 17;470(1):51-4.

[PubMed ID: 8226790] ... Liu QR, Lopez-Corcuera B, Mandiyan S, Nelson H, Nelson N, Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features. J Biol Chem. 1993 Oct 25;268(30):22802-8.