SB0016 : Connexin-49, Gap junction [alpha]-8
[ CaMP Format ]
* Basic Information
| Organism | Ovis aries (sheep) |
| Protein Names | connexin 49 protein [Ovis aries]; connexin 49 protein; MP70; similar to Mus musculus connexin 50, Gallus gallu connexin 45.6, Homo sapiens connexin 50 proteins; Gap junction alpha-8 protein; Connexin-49; Cx49; Lens fiber protein MP70; MP38; MP64 |
| Gene Names | GJA8 |
| Gene Locus | Not available |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 440 aa
Average Mass: 49.145 kDa
Monoisotopic Mass: 49.115 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
| Bartter syndrome, infantile, with sensorineural deafness (Barttin) 1. | 96 | 147 | 131.0 | 0.1 |
| --- cleavage 290 --- |
| --- cleavage 300 --- |
| Connexin 40 C-terminal domain 1. | 400 | 440 | 79.0 | 0.0 |
3. Sequence Information
Fasta Sequence: SB0016.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
2 [sites]
Source Reference: [PubMed ID: 9208227] Lin JS, Fitzgerald S, Dong Y, Knight C, Donaldson P, Kistler J, Processing of the gap junction protein connexin50 in the ocular lens is accomplished by calpain. Eur J Cell Biol. 1997 Jun;73(2):141-9.
Cleavage sites (±10aa)
[Site 1] IVSHYFPLTE290-VGMVEASPLS
Glu290 
Val
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Ile281 | Val282 | Ser283 | His284 | Tyr285 | Phe286 | Pro287 | Leu288 | Thr289 | Glu290 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Val291 | Gly292 | Met293 | Val294 | Glu295 | Ala296 | Ser297 | Pro298 | Leu299 | Ser300 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
SIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVEASPLSAKPFSQFEEKVGPGPLGDLS
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
120.00 |
1 |
PREDICTED: similar to Gap junction alpha-8 protei |
| 2 |
N/A |
120.00 |
1 |
CXA8_SHEEP Gap junction alpha-8 protein (Connexin |
| 3 |
Ovis aries |
120.00 |
1 |
AF177913_1 connexin 49 protein |
| 4 |
Canis familiaris |
115.00 |
6 |
PREDICTED: similar to gap junction membrane chann |
| 5 |
Mus musculus |
113.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 6 |
Rattus norvegicus |
113.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 7 |
Homo sapiens |
109.00 |
2 |
connexin 50 |
| 8 |
Pan troglodytes |
109.00 |
1 |
PREDICTED: gap junction protein, alpha 8, 50kDa ( |
| 9 |
Gallus gallus |
80.50 |
1 |
connexin 50 |
| 10 |
Danio rerio |
62.40 |
2 |
AF288817_1 gap junction protein connexin 44.1 |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
|
| N/A |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
|
| Ovis aries |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
|
| Canis familiaris |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| |||||||
Sbjct 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKMGTGPLGDLS 320
|
| Mus musculus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| ||| |+|
Sbjct 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKIGTGPLADMS 320
|
| Rattus norvegicus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||||||||||||||+| ||| |+|
Sbjct 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLSAKPFSQFEEKIGTGPLADMS 320
|
| Homo sapiens |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||| ||||+|||||+ |||||||
Sbjct 254 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLPAKPFNQFEEKISTGPLGDLS 313
|
| Pan troglodytes |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
||||||||||||||||||||||||||||||#||||| ||| ||||+|||||+ |||||||
Sbjct 254 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVETSPLPAKPFNQFEEKISTGPLGDLS 313
|
| Gallus gallus |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEKVGPGPLGDLS 320
+|||||| |||||+|||||| |||||||||#|| || ||| + |++||||+| ||| |||
Sbjct 256 AIAVSSIPKAKGYKLLEEEKPVSHYFPLTE#VG-VEPSPLPSA-FNEFEEKIGMGPLEDLS 313
|
| Danio rerio |
Query 261 SIAVSSIQKAKGYQLLEEEKIVSHYFPLTE#VGMVEASPLSAKPFSQFEEK 310
||| |||||||||+||||+| ||+|||||#|| +|| | | + ||||
Sbjct 262 SIAASSIQKAKGYKLLEEDKSTSHFFPLTE#VGGMEAGRLPAS-YEPFEEK 310
|
[Site 2] VGMVEASPLS300-AKPFSQFEEK
Ser300 Ala
iTraq-117 Signal 3942.1 (
) for AKPFSQFEEK
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Val291 | Gly292 | Met293 | Val294 | Glu295 | Ala296 | Ser297 | Pro298 | Leu299 | Ser300 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala301 | Lys302 | Pro303 | Phe304 | Ser305 | Gln306 | Phe307 | Glu308 | Glu309 | Lys310 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
KGYQLLEEEKIVSHYFPLTEVGMVEASPLSAKPFSQFEEKVGPGPLGDLSRAYQETLPSY
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
123.00 |
1 |
PREDICTED: similar to Gap junction alpha-8 protei |
| 2 |
Ovis aries |
123.00 |
1 |
AF177913_1 connexin 49 protein |
| 3 |
N/A |
123.00 |
1 |
CXA8_SHEEP Gap junction alpha-8 protein (Connexin |
| 4 |
Canis familiaris |
118.00 |
6 |
PREDICTED: similar to gap junction membrane chann |
| 5 |
Mus musculus |
115.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 6 |
Rattus norvegicus |
114.00 |
1 |
gap junction membrane channel protein alpha 8 |
| 7 |
Homo sapiens |
110.00 |
2 |
connexin 50 |
| 8 |
Pan troglodytes |
110.00 |
1 |
PREDICTED: gap junction protein, alpha 8, 50kDa ( |
| 9 |
Gallus gallus |
81.30 |
1 |
connexin 50 |
| 10 |
Danio rerio |
60.80 |
2 |
AF288817_1 gap junction protein connexin 44.1 |
| 11 |
Tetraodon nigroviridis |
49.30 |
1 |
unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|
| Ovis aries |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|
| N/A |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|
| Canis familiaris |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| |||||||||||||||||
Sbjct 271 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKMGTGPLGDLSRAYQETLPSY 330
|
| Mus musculus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| ||| |+||+||||||||
Sbjct 271 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKIGTGPLADMSRSYQETLPSY 330
|
| Rattus norvegicus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||||#||||||||||+| ||| |+|| ||||||||
Sbjct 271 KGYQLLEEEKIVSHYFPLTEVGMVETSPLS#AKPFSQFEEKIGTGPLADMSRGYQETLPSY 330
|
| Homo sapiens |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||| #||||+|||||+ |||||||| ||||||||
Sbjct 264 KGYQLLEEEKIVSHYFPLTEVGMVETSPLP#AKPFNQFEEKISTGPLGDLSRGYQETLPSY 323
|
| Pan troglodytes |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
||||||||||||||||||||||||| ||| #||||+|||||+ |||||||| ||||||||
Sbjct 264 KGYQLLEEEKIVSHYFPLTEVGMVETSPLP#AKPFNQFEEKISTGPLGDLSRGYQETLPSY 323
|
| Gallus gallus |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPGPLGDLSRAYQETLPSY 330
|||+|||||| ||||||||||| || ||| #+ |++||||+| ||| |||||+ | ||||
Sbjct 266 KGYKLLEEEKPVSHYFPLTEVG-VEPSPLP#SA-FNEFEEKIGMGPLEDLSRAFDERLPSY 323
|
| Danio rerio |
Query 271 KGYQLLEEEKIVSHYFPLTEVGMVEASPLS#AKPFSQFEEKV--GPGPLGDLSRAYQETLP 328
|||+||||+| ||+||||||| +|| | #| + |||| | |+|+ | ||||
Sbjct 272 KGYKLLEEDKSTSHFFPLTEVGGMEAGRLP#AS-YEPFEEKSDEAMAPKKDMSKMYDETLP 330
Query 329 SY 330
||
Query 329 SY 330
|
| Tetraodon nigroviridis |
Query 271 KGYQLLEEEKI--VSHYFPLTEVGMVEASPLS#AKPFSQFEEKVGPG---PLGDLSRAYQE 325
|||+|||||| ++| +|| |||| #+ || ||| | |+ |+|+ | |
Sbjct 278 KGYRLLEEEKAPPITHLYPLAEVGMEAGR--G#SPPFQGLEEK--PEEVLPMEDISKVYDE 333
Query 326 TLPSY 330
|||||
Query 326 TLPSY 330
|
* References
[PubMed ID: 8654111] Yang DI, Louis CF, Molecular cloning of sheep connexin49 and its identity with MP70. Curr Eye Res. 1996 Mar;15(3):307-14.
