CaMPDB :: Substrate

SB0019 : IL-1 [alpha], Interleukin 1 [alpha]

* Basic Information

Organism	Homo sapiens (human)
Protein Names	interleukin-1 alpha proprotein [Homo sapiens]; interleukin-1 alpha proprotein; preinterleukin 1 alpha; hematopoietin-1; interleukin-1 alpha; pro-interleukin-1-alpha; IL-1 alpha; Interleukin-1 alpha; Hematopoietin-1
Gene Names	IL1A; IL1F1; interleukin 1, alpha
Gene Locus	2q14; chromosome 2
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_000566	NM_000575	3552	P01583	147760	HGNC:5991	N/A	hsa:3552

* Information From OMIM

Description: IL1A is 1 of 2 structurally distinct forms of IL1, the other being IL1B (OMIM:147720). The IL1A and IL1B proteins are synthesized by a variety of cell types, including activated macrophages, keratinocytes, stimulated B lymphocytes, and fibroblasts, and are potent mediators of inflammation and immunity (Lord et al., 1991).

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* Structure Information

1. Primary Information

Length: 271 aa

Average Mass: 30.606 kDa

Monoisotopic Mass: 30.587 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Interleukin-1 propeptide 1.	1	109	1.0	2.3
--- cleavage 118 ---
Interleukin-1 / 18 1.	157	266	3.0	0.0

3. Sequence Information

Fasta Sequence: SB0019.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1ITA (Model; -; A=121-271), 2ILA (X-ray; 230 A; A=117-271), 2KKI (NMR; -; A=121-271), 2L5X (NMR; -; A/D=121-271)

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 2115174] Kobayashi Y, Yamamoto K, Saido T, Kawasaki H, Oppenheim JJ, Matsushima K, Identification of calcium-activated neutral protease as a processing enzyme of human interleukin 1 alpha. Proc Natl Acad Sci U S A. 1990 Jul;87(14):5548-52.

Cleavage sites (±10aa)

[Site 1] IKPRSAPFSF¹¹⁸-LSNVKYNFMR

Phe¹¹⁸ Leu

iTraq-117 Signal 5847.2 () for IKPRSAPFSF

iTraq-117 Signal 22261.8 () for LSNVKYNFMR

iTraq-117 Signal 6555.0 () for NVKYNFMR

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile¹⁰⁹	Lys¹¹⁰	Pro¹¹¹	Arg¹¹²	Ser¹¹³	Ala¹¹⁴	Pro¹¹⁵	Phe¹¹⁶	Ser¹¹⁷	Phe¹¹⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu¹¹⁹	Ser¹²⁰	Asn¹²¹	Val¹²²	Lys¹²³	Tyr¹²⁴	Asn¹²⁵	Phe¹²⁶	Met¹²⁷	Arg¹²⁸

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SQSITDDDLEAIANDSEEEIIKPRSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRAN

Summary

#	organism	max score	hits	top seq
1	synthetic construct	122.00	3	Homo sapiens interleukin 1, alpha
2	Homo sapiens	122.00	2	interleukin 1, alpha proprotein
3	Gorilla gorilla	119.00	1	AF354636_1 interleukin alpha 1
4	Dasypus novemcinctus	116.00	1	IL-1 alpha-like protein
5	Macaca mulatta	107.00	1	interleukin 1, alpha
6	Macaca fascicularis	107.00	1	IL1A_MACFA Interleukin-1 alpha precursor (IL-1 al
7	N/A	101.00	3	IL1A_CERTO Interleukin-1 alpha precursor (IL-1 al
8	Ovis aries	96.70	1	interleukin 1, alpha
9	Capra hircus	96.70	1	IL1A_CAPHI Interleukin-1 alpha precursor (IL-1 al
10	Bubalus carabanensis	94.70	2	Interleukin-1 alpha
11	Bubalus bubalis	94.70	1	Interleukin-1 alpha
12	Bos taurus	94.70	1	interleukin 1, alpha
13	Sus scrofa prointerleukin-1 alpha mRNA, complete cds.	92.80	1	-
14	Sus scrofa	92.80	1	interleukin 1, alpha
15	Lama glama	92.40	1	IL1A_LAMGL Interleukin-1 alpha precursor (IL-1 al
16	Camelus bactrianus	90.50	1	interleukin 1 alpha
17	Tursiops truncatus	85.10	1	interleukin-1 alfa
18	Equus caballus	84.70	2	interleukin-1 alpha
19	Canis familiaris	83.60	1	IL1A_CANFA Interleukin-1 alpha precursor (IL-1 al
20	Oryctolagus cuniculus	76.60	1	interleukin 1, alpha
21	Felis catus	72.80	1	interleukin 1, alpha
22	Rattus norvegicus	70.50	1	interleukin 1 alpha
23	Mus musculus	64.70	1	interleukin 1 alpha
24	Sigmodon hispidus	55.50	1	AF398548_1 interleukin 1 alpha
25	Mesocricetus auratus	52.80	1	interleukin-1 alpha

Top-ranked sequences

organism	matching
synthetic construct	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148
Homo sapiens	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148
Gorilla gorilla	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 78 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKNEFILNDALNQSIIRAN 137
Dasypus novemcinctus	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|+ \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 22 SQSLADDDLEAIDNDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 81
Macaca mulatta	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+ \|+\|+\|\|\|\|+\|\|\|\|\|\| \|\|\|+\|\|\|\|\| Sbjct 89 SQSITDNNLEAIANDSEEEIIKPRSAPFSF#LSNMTYHFIRIIKHEFILNDTLNQTIIRAN 148
Macaca fascicularis	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+ \|+\|+\|\|\|\|+\|\|\|\|\|\| \|\|\|+\|\|\|\|\| Sbjct 89 SQSITDNNLEAIANDSEEEIIKPRSAPFSF#LSNMTYHFIRIIKHEFILNDTLNQTIIRAN 148
N/A	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIRAN 148 \|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|+ \|+ +\|\|\|\|+\| \|\|\|\| \|\|\|+\|\|\|\|\| Sbjct 89 SQSIADDNLEAIANDSEEEIIKPRSAPFSF#LSNMTYHIIRIIKHESILNDTLNQTIIRAN 148
Ovis aries	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANDTEEEIIKPRSAHYSF#QSNVKYNFMRVIHQECILNDALNQSIIR 146
Capra hircus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANDTEEEIIKPRSAHYSF#QSNVKYNFMRVIHQECILNDALNQSIIR 146
Bubalus carabanensis	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANNTEEEIIKPRSAHYSF#QSNVKYNFMRVIHQECILNDALNQSIIR 146
Bubalus bubalis	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANNTEEEIIKPRSAHYSF#QSNVKYNFMRVIHQECILNDALNQSIIR 146
Bos taurus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|++\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|\|\|\|\|\|\|\|+\| \| \|\|\|\|\|\|\|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANNTEEEIIKPRSAHYSF#QSNVKYNFMRVIHQECILNDALNQSIIR 146
Sus scrofa prointerleukin-1 alpha mRNA, complete cds.	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|+\|\|\|\|\|\|+\| ++ \|\|\|\|\| \|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANDTEEEIIKPRSATYSF#QSNMKYNFMRVINHQCILNDARNQSIIR 146
Sus scrofa	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|+\|\|\|\|\|\|+\| ++ \|\|\|\|\| \|\|\|\|\|\| Sbjct 90 QFITDDDLEAIANDTEEEIIKPRSATYSF#QSNMKYNFMRVINHQCILNDARNQSIIR 146
Lama glama	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\| \|++ # \|\|\|\|\|\|+\|\|\|+ ++ \|\|\|\|\|\|\|+\|\|\|\| Sbjct 90 QFITDDDLEAIANDTEEEIIKPRSVPYNL#QSNVKYNYMRIVNHQCILNDALNRSIIR 146
Camelus bactrianus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|+\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\| \|++ # \|\|\|\|\|\|+\|\|\|+ ++ \|\|\|\|\|\|\|+\|\|\|\| Sbjct 90 QFITNDDLEAIANDTEEEIIKPRSVPYNL#QSNVKYNYMRIVNHQCILNDALNRSIIR 146
Tursiops truncatus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSII 145 \| +\|\|\|\|\|\| \|\|\|\|+\|\|\|\|\|\|\|\|\|\| +\|\|# \|\|+\|\|\|\|+++\|+ ++\|\|\|\|\|\|+\|\|\|+ Sbjct 90 QFLTDDDLEDIANDTEEEIIKPRSAHYSF#QSNMKYNFIKLIQQQYILNDALHQSIV 145
Equus caballus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|+\|\|\|\|\|\|\|\|\| \|\| \|\|\|\|\|\| ++\|# \|\| \|\|\|\|\|\|\|+ ++ \|\|\|\|\|\|\|\|+\|\| Sbjct 90 QFITNDDLEAIANDPEEGIIKPRSVHYNF#QSNTKYNFMRIVNHQCTLNDALNQSVIR 146
Canis familiaris	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \|\| \|\|\|\|\|\|\| \|\|\|\|+\|\| \|+\|\|\|\| ++\|# +\| \|\|\|++\|\|\|\| +\|\|\|\|\| \|\|\|\|\|+\| Sbjct 85 SQFITDDDLEGIANDTEEVIMKPRSVAYNF#HNNEKYNYIRIIKSQFILNDNLNQSIVR 142
Oryctolagus cuniculus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| \|\|\| \|\|\| +\| \|\| \|\|\|\|\|\| \|++\|# \|++\| ++\|\|\|\| \|\| \|\|\|\|\|\|\|\|++\| Sbjct 90 QPITDVDLETNVSDPEEGIIKPRSVPYTF#QRNMRYKYLRIIKQEFTLNDALNQSLVR 146
Felis catus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \| +\| \|\|\|\|\|\|\|\|+ \|\|\|\|+\|\|\|\| +\|# \|+ \|\|\|+ +\|\|\| +\|\|\|\|\| \|+\|\|+\|\| Sbjct 90 QFLTADDLEAIANEVEEEIMKPRSVAPNF#YSSEKYNYQKIIKSQFILNDNLSQSVIR 146
Rattus norvegicus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSI 144 \| \|+\|\|\|\|\|\|\|+\| \|\| \|+\|\|\|\|\| \|\|# +\|++\| +\|\|+\| \|\|\|+\|\|+\|\|\|+\| Sbjct 93 QPFTEDDLEAIAHDLEE-TIQPRSAPHSF#QNNLRYKLIRIVKQEFIMNDSLNQNI 146
Mus musculus	Query 89 SQSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSIIR 146 \|++ \|+\|\|\|++\| +\| \|\| \|+\|\|\|\|\|+++# \|+++\| \|++++ +\|++\|\|+\|\|\|+\| + Sbjct 92 SETFTEDDLQSITHDLEE-TIQPRSAPYTY#QSDLRYKLMKLVRQKFVMNDSLNQTIYQ 148
Sigmodon hispidus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSI 144 \|+ +\|\|\|\|\|\| + \|\| \|+ \|\|\| \|# \|\|++\| +\|++\| \|\|+\|\|\|+\|+\|+\| Sbjct 93 QAFAEDDLEAITH-GLEETIQSDSAPRLF#QSNMRYKLLRLVKQEFVLNDSLHQNI 146
Mesocricetus auratus	Query 90 QSITDDDLEAIANDSEEEIIKPRSAPFSF#LSNVKYNFMRIIKYEFILNDALNQSI 144 \|+ +\|\|\|\| \| + \|\| \|+ \|\|\|+ \|# \|\|++\| +\| + \|\|+\|\|\|+\|\|\|+\| Sbjct 63 QAFAEDDLETITRNLEE-TIQSDSAPYVF#QSNMRYKLIRRVMQEFVLNDSLNQNI 116

* References

[PubMed ID: 24613844] Kwon MJ, Hong E, Choi Y, Kang DH, Oh ES, Interleukin-1alpha promotes extracellular shedding of syndecan-2 via induction of matrix metalloproteinase-7 expression. Biochem Biophys Res Commun. 2014 Apr 4;446(2):487-92. doi:

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[PubMed ID: 24275344] ... Zuccarello D, Bazzato MF, Ferlin A, Pengo M, Frigo AC, Favero G, Foresta C, Stellini E, Role of familiarity versus interleukin-1 genes cluster polymorphisms in chronic periodontitis. Gene. 2014 Feb 10;535(2):286-9. doi: 10.1016/j.gene.2013.11.016. Epub 2013 Nov

[PubMed ID: 24313836] ... Sulik A, Kroten A, Wojtkowska M, Oldak E, Increased levels of cytokines in cerebrospinal fluid of children with aseptic meningitis caused by mumps virus and echovirus 30. Scand J Immunol. 2014 Jan;79(1):68-72. doi: 10.1111/sji.12131.

[PubMed ID: 24265545] ... Borkenstein A, Faschinger C, Maier R, Weger M, Theisl A, Demel U, Graninger W, Irene H, Mossbock G, Measurement of tumor necrosis factor-alpha, interleukin-6, Fas ligand, interleukin-1alpha, and interleukin-1beta in the aqueous humor of patients with open angle glaucoma using multiplex bead analysis. Mol Vis. 2013 Nov 16;19:2306-11. eCollection 2013.

[PubMed ID: 24273934] ... Wu T, Du R, Hong Y, Jia L, Zeng Q, Cheng B, IL-1 alpha regulates CXCL1, CXCL10 and ICAM1 in network form in oral keratinocytes. Clin Lab. 2013;59(9-10):1105-11.

[PubMed ID: 7499244] ... Kavita U, Mizel SB, Differential sensitivity of interleukin-1 alpha and -beta precursor proteins to cleavage by calpain, a calcium-dependent protease. J Biol Chem. 1995 Nov 17;270(46):27758-65.

[PubMed ID: 1584804] ... Kumar S, Millis AJ, Baglioni C, Expression of interleukin 1-inducible genes and production of interleukin 1 by aging human fibroblasts. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4683-7.

[PubMed ID: 1739124] ... Clinton SK, Underwood R, Hayes L, Sherman ML, Kufe DW, Libby P, Macrophage colony-stimulating factor gene expression in vascular cells and in experimental and human atherosclerosis. Am J Pathol. 1992 Feb;140(2):301-16.

[PubMed ID: 1924307] ... Hogquist KA, Nett MA, Unanue ER, Chaplin DD, Interleukin 1 is processed and released during apoptosis. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8485-9.

[PubMed ID: 1675504] ... Ishikura H, Takahashi C, Kanagawa K, Hirata H, Imai K, Yoshiki T, Cytokine regulation of ICAM-1 expression on human renal tubular epithelial cells in vitro. Transplantation. 1991 Jun;51(6):1272-5.

[PubMed ID: 2346741] ... Graves BJ, Hatada MH, Hendrickson WA, Miller JK, Madison VS, Satow Y, Structure of interleukin 1 alpha at 2.7-A resolution. Biochemistry. 1990 Mar 20;29(11):2679-84.

[PubMed ID: 3126184] ... Beuscher HU, Nickells MW, Colten HR, The precursor of interleukin-1 alpha is phosphorylated at residue serine 90. J Biol Chem. 1988 Mar 15;263(8):4023-8.