logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0023 : IGFBP-5, Insulin-like growth factor binding protein 5

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesinsulin-like growth factor-binding protein 5 precursor [Homo sapiens]; insulin-like growth factor-binding protein 5 precursor; insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5; Insulin-like growth factor-binding protein 5
Gene NamesIGFBP5; IBP5; insulin-like growth factor binding protein 5
Gene Locus2q35; chromosome 2
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_000590 NM_000599 3488 P24593 146734 HGNC:5474 00901 hsa:3488

* Information From OMIM

Function: Sato et al. (2002) overexpressed a constitutively active form of Six5 (OMIM:600963) in murine P19 embryonal carcinoma cells. Using expression profiling in cDNA arrays, they identified 21 potential target genes whose expression level increased by the treatment. One of these genes was Igfbp5, which was also decreased in Six5-deficient mouse fibroblasts. The response of human IGFBP5 to MyoD (OMIM:159970)-induced muscle conversion was altered in cells of myotonic dystrophy-1 (DM1; OMIM:160900) patients. The authors concluded that Six5 is an activator that directs Igfbp5 expression, and hypothesized that reduced SIX5 expression in DM1 may contribute to the DM1 phenotype.

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* Structure Information

1. Primary Information

Length: 272 aa

Average Mass: 30.570 kDa

Monoisotopic Mass: 30.550 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 22 ---
Insulin-like growth factor binding protein 1. 27801.022.1
--- cleavage 161 ---
--- cleavage 172 ---
Insulin-like growth factor binding protein 2. 23023412.00.6

3. Sequence Information

Fasta Sequence: SB0023.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1BOE (NMR; -; A=60-106), 1H59 (X-ray; 210 A; B=60-112)

* Cleavage Information

3 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 15843151] Ghosh M, Shanker S, Siwanowicz I, Mann K, Machleidt W, Holak TA, Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain. Biol Chem. 2005 Jan;386(1):85-93.

Cleavage sites (±10aa)

[Site 1] AYAGPAQSLG22-SFVHCEPCDE

Gly22 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala13Tyr14Ala15Gly16Pro17Ala18Gln19Ser20Leu21Gly22
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser23Phe24Val25His26Cys27Glu28Pro29Cys30Asp31Glu32

Sequence conservation (by blast)

[Site 2] VKKDRRKKLT161-QSKFVGGAEN

Thr161 Gln

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Val152Lys153Lys154Asp155Arg156Arg157Lys158Lys159Leu160Thr161
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gln162Ser163Lys164Phe165Val166Gly167Gly168Ala169Glu170Asn171

Sequence conservation (by blast)

[Site 3] SKFVGGAENT172-AHPRIISAPE

Thr172 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser163Lys164Phe165Val166Gly167Gly168Ala169Glu170Asn171Thr172
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala173His174Pro175Arg176Ile177Ile178Ser179Ala180Pro181Glu182

Sequence conservation (by blast)

* References

[PubMed ID: 24379630] Adali G, Yorulmaz E, Ozkanli S, Ulasoglu C, Bayraktar B, Orhun A, Colak Y, Tuncer I, Serum concentrations of insulin-like growth factor-binding protein 5 in Crohn's disease. World J Gastroenterol. 2013 Dec 21;19(47):9049-56. doi: 10.3748/wjg.v19.i47.9049.

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