logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0025 : S-arrestin

[ CaMP Format ]

* Basic Information

OrganismBos taurus (cattle)
Protein NamesS-antigen [Bos taurus]; S-arrestin; 48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin; S-arrestin short form
Gene NamesSAG
Gene LocusNot available
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
AAA30377 N/A N/A P08168 N/A N/A N/A bta:280922

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 404 aa

Average Mass: 45.310 kDa

Monoisotopic Mass: 45.283 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Arrestin (or S-antigen), N-terminal domain 1. 221791.00.0
Predicted membrane protein (DUF2079) 1. 236289341.00.1

3. Sequence Information

Fasta Sequence: SB0025.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1AYR (X-ray; 330 A; A/B/C/D=1-367), 1CF1 (X-ray; 280 A; A/B/C/D=2-404), 1Y6Y (Model; -; B=10-362), 3UGU (X-ray; 185 A; A=1-370), 3UGX (X-ray; 265 A; A/B/C/D=1-404), 4J2Q (X-ray; 300 A; A/B=1-369), 4PXF (X-ray; 275 A; B=67-77)

* Cleavage Information

2 [sites]

Source Reference: [PubMed ID: 7592650] Azarian SM, King AJ, Hallett MA, Williams DS, Selective proteolysis of arrestin by calpain. Molecular characteristics and its effect on rhodopsin dephosphorylation. J Biol Chem. 1995 Oct 13;270(41):24375-84.

Cleavage sites (±10aa)

[Site 1] ESFQDENFVF377-EEFARQNLKD

Phe377 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Glu368Ser369Phe370Gln371Asp372Glu373Asn374Phe375Val376Phe377
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Glu378Glu379Phe380Ala381Arg382Gln383Asn384Leu385Lys386Asp387

Sequence conservation (by blast)

[Site 2] VFEEFARQNL385-KDAGEYKEEK

Leu385 Lys

iTraq-117 Signal 283.1 () for KDAGEYKEEK

iTraq-117 Signal 1198.2 () for DAGEYKEEK

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Val376Phe377Glu378Glu379Phe380Ala381Arg382Gln383Asn384Leu385
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys386Asp387Ala388Gly389Glu390Tyr391Lys392Glu393Glu394Lys395

Sequence conservation (by blast)

* References

[PubMed ID: 2950857] Yamaki K, Takahashi Y, Sakuragi S, Matsubara K, Molecular cloning of the S-antigen cDNA from bovine retina. Biochem Biophys Res Commun. 1987 Feb 13;142(3):904-10.