* Cleavage Information
1 [sites] cleaved by Calpain 1 and 2
Source Reference: [PubMed ID: 6092335] Sasaki T, Kikuchi T, Yumoto N, Yoshimura N, Murachi T, Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. J Biol Chem. 1984 Oct 25;259(20):12489-94.
Cleavage sites (±10aa)
[Site 1] VSLTAGDRVY28-IHPFHLLYYS
Tyr28 
Ile
iTraq-117 Signal 2952.8 (
) for SLTAGDRVYIHPFHLLY
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Val19 | Ser20 | Leu21 | Thr22 | Ala23 | Gly24 | Asp25 | Arg26 | Val27 | Tyr28 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ile29 | His30 | Pro31 | Phe32 | His33 | Leu34 | Leu35 | Tyr36 | Tyr37 | Ser38 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MTPTGAGLKATIFCILTWVSLTAGDRVYIHPFHLLYYSKSTCAQLENPSVETLPEPTF
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Rattus norvegicus |
126.00 |
1 |
angiotensinogen |
| 2 |
Contains: Angiotensin-1 (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin III) (Ang III) (Des-Asp[1 |
119.00 |
4 |
ANGT_MOUSE Angiotensinogen precursor (Serpin A8) |
| 3 |
Mus musculus |
119.00 |
2 |
Agt protein |
| 4 |
Bos taurus |
89.70 |
1 |
angiotensinogen (serpin peptidase inhibitor, clad |
| 5 |
Contains: Angiotensin-1 (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin III) (Ang III) (Des-Asp[1 |
85.50 |
1 |
ANGT_SHEEP Angiotensinogen precursor (Serpin A8) |
| 6 |
N/A |
84.00 |
3 |
2022178A angiotensinogen |
| 7 |
Homo sapiens |
73.60 |
2 |
Angiotensinogen (serpin peptidase inhibitor, clad |
| 8 |
Homo sapiens |
73.60 |
1 |
angiotensinogen preproprotein |
| 9 |
synthetic construct |
73.60 |
1 |
Homo sapiens angiotensinogen (serine (or cysteine |
| 10 |
Pan troglodytes |
72.00 |
2 |
angiotensinogen |
| 11 |
Canis familiaris |
60.10 |
2 |
PREDICTED: similar to Angiotensinogen precursor |
| 12 |
Contains: Angiotensin-1 (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin III) (Ang III) (Des-Asp[1 |
31.60 |
1 |
ANGT_HORSE Angiotensinogen (Serpin A8) |
Top-ranked sequences
| organism | matching |
|---|
| Rattus norvegicus |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
|
| Contains: Angiotensin-1 (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin III) (Ang III) (Des-Asp[1 |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
||||||||||||||||||||||||||||#||||||||++||||||||||||||||| ||
Sbjct 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYHNKSTCAQLENPSVETLPESTF 58
|
| Mus musculus |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
||||||||||||||||||||||||||||#||||||||++||||||||||||||||| ||
Sbjct 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYHNKSTCAQLENPSVETLPESTF 58
|
| Bos taurus |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | | | | |+| | | ||||||#+|||||| |||| | ||| ||||| |+|||
Sbjct 1 MAPAGLSLGAAILCLLAWAGLAAGDRVY#VHPFHLLVYSKSNCDQLEKPSVETPPDPTF 58
|
| Contains: Angiotensin-1 (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin III) (Ang III) (Des-Asp[1 |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | | ||| |+| | | ||||||#||||||| +||| | ||| ||||| +||
Sbjct 1 MAPAGLSLGATILCLLAWAGLAAGDRVY#IHPFHLLVHSKSNCDQLEKPSVETPADPTL 58
|
| N/A |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | | ||| |+| | | ||||||#||||||| +||| | ||| |||| +||
Sbjct 1 MAPAGLSLGATILCLLAWAGLAAGDRVY#IHPFHLLVHSKSNCDQLEKPSVEPTADPTL 58
|
| Homo sapiens |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | |+||| |+| | | ||||||#||||||+ +++||| || + +|||
Sbjct 10 MAPAGVSLRATILCLLAWAGLAAGDRVY#IHPFHLVIHNESTCEQLAKANAGKPKDPTF 67
|
| Homo sapiens |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | |+||| |+| | | ||||||#||||||+ +++||| || + +|||
Sbjct 10 MAPAGVSLRATILCLLAWAGLAAGDRVY#IHPFHLVIHNESTCEQLAKANAGKPKDPTF 67
|
| synthetic construct |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | |+||| |+| | | ||||||#||||||+ +++||| || + +|||
Sbjct 10 MAPAGVSLRATILCLLAWAGLAAGDRVY#IHPFHLVIHNESTCEQLAKANAGKPKDPTF 67
|
| Pan troglodytes |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | |+||| |++ | | ||||||#||||||+ +++||| || + +|||
Sbjct 10 MAPAGVSLRATILCLVAWAGLAAGDRVY#IHPFHLVIHNESTCEQLAKANAGKPKDPTF 67
|
| Canis familiaris |
Query 1 MTPTGAGLKATIFCILTWVSLTAGDRVY#IHPFHLLYYSKSTCAQLENPSVETLPEPTF 58
| | | | |+ |+| | ||||||#||||||| ||||+| ||| + | + ||
Sbjct 15 MAPAGVSLVVTVLCLLAGAGLAAGDRVY#IHPFHLLVYSKSSCEQLEKSNTEMPKDLTF 72
|
| Contains: Angiotensin-1 (Angiotensin I) (Ang I); Angiotensin-2 (Angiotensin II) (Ang II); Angiotensin-3 (Angiotensin III) (Ang III) (Des-Asp[1 |
Query 25 DRVY#IHPFHLLYYS 38
||||#||||||| ||
Sbjct 1 DRVY#IHPFHLLVYS 14
|
* References
[PubMed ID: 24191285] Wang F, Demura M, Cheng Y, Zhu A, Karashima S, Yoneda T, Demura Y, Maeda Y, Namiki M, Ono K, Nakamura Y, Sasano H, Akagi T, Yamagishi M, Saijoh K, Takeda Y, Dynamic CCAAT/enhancer binding protein-associated changes of DNA methylation in the angiotensinogen gene. Hypertension. 2014 Feb;63(2):281-8. doi: 10.1161/HYPERTENSIONAHA.113.02303. Epub
» More (9) « Hide Below
[PubMed ID: 24481965] ... Chiu CZ, Wang BW, Shyu KG, Angiotensin II and the JNK pathway mediate urotensin II expression in response to hypoxia in rat cardiomyocytes. J Endocrinol. 2014 Jan 30;220(3):233-46. doi: 10.1530/JOE-13-0261. Print 2014
[PubMed ID: 24184051] ... Garrido-Gil P, Rodriguez-Pallares J, Dominguez-Meijide A, Guerra MJ, Labandeira-Garcia JL, Brain angiotensin regulates iron homeostasis in dopaminergic neurons and microglial cells. Exp Neurol. 2013 Dec;250:384-96. doi: 10.1016/j.expneurol.2013.10.013. Epub 2013
[PubMed ID: 23832698] ... Sharma NM, Llewellyn TL, Zheng H, Patel KP, Angiotensin II-mediated posttranslational modification of nNOS in the PVN of rats with CHF: role for PIN. Am J Physiol Heart Circ Physiol. 2013 Sep 15;305(6):H843-55. doi:
[PubMed ID: 23891689] ... Kim MS, Oh KS, Lee JH, Ryu SY, Mun J, Lee BH, Kamolonol suppresses angiotensin II-induced stress fiber formation and cellular hypertrophy through inhibition of Rho-associated kinase 2 activity. Biochem Biophys Res Commun. 2013 Aug 23;438(2):318-23. doi:
[PubMed ID: 1394429] ... Jeunemaitre X, Soubrier F, Kotelevtsev YV, Lifton RP, Williams CS, Charru A, Hunt SC, Hopkins PN, Williams RR, Lalouel JM, et al., Molecular basis of human hypertension: role of angiotensinogen. Cell. 1992 Oct 2;71(1):169-80.
[PubMed ID: 3745432] ... Tucker BJ, Mundy CA, Maciejewski AR, Printz MP, Ziegler MG, Pelayo JC, Blantz RC, Changes in glomerular hemodynamic response to angiotensin II after subacute renal denervation in rats. J Clin Invest. 1986 Sep;78(3):680-8.
[PubMed ID: 3947969] ... Rettig R, Healy DP, Printz MP, Cardiovascular effects of microinjections of angiotensin II into the nucleus tractus solitarii. Brain Res. 1986 Feb 5;364(2):233-40.
[PubMed ID: 3753601] ... Ohkubo H, Nakayama K, Tanaka T, Nakanishi S, Tissue distribution of rat angiotensinogen mRNA and structural analysis of its heterogeneity. J Biol Chem. 1986 Jan 5;261(1):319-23.
[PubMed ID: 4030046] ... Healy DP, Printz MP, Angiotensinogen levels in the brain and cerebrospinal fluid of the genetically hypertensive rat. Hypertension. 1985 Sep-Oct;7(5):752-9.
