CaMPDB :: Substrate

SB0034 : Neurotensin

* Basic Information

Organism	Bos taurus (cattle)
Protein Names	neurotensin/neuromedin N precursor [Bos taurus]; neurotensin/neuromedin N precursor; neurotensin/neuromedin N; Neurotensin/neuromedin N; Large neuromedin N; NmN-125; Neuromedin N; NN; NmN; Neurotensin; NT; Tail peptide
Gene Names	NTS; neurotensin
Gene Locus	5q13; chromosome 5
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_776370	NM_173945	280881	P01156	N/A	N/A	N/A	bta:280881

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 170 aa

Average Mass: 19.843 kDa

Monoisotopic Mass: 19.830 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Neurotensin/neuromedin N precursor 1.	5	169	2.0	1.3
--- cleavage 153 (inside Neurotensin/neuromedin N precursor 5..169) ---
--- cleavage 158 (inside Neurotensin/neuromedin N precursor 5..169) ---

3. Sequence Information

Fasta Sequence: SB0034.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

2 [sites] cleaved by Calpain 1 and 2

Source Reference: [PubMed ID: 6092335] Sasaki T, Kikuchi T, Yumoto N, Yoshimura N, Murachi T, Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. J Biol Chem. 1984 Oct 25;259(20):12489-94.

Cleavage sites (±10aa)

[Site 1] IPYILKRQLY¹⁵³-ENKPRRPYIL

Tyr¹⁵³ Glu

iTraq-117 Signal 5813.9 () for IPYILKRQLY

iTraq-117 Signal 5031.6 () for ENKPRRPYIL

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile¹⁴⁴	Pro¹⁴⁵	Tyr¹⁴⁶	Ile¹⁴⁷	Leu¹⁴⁸	Lys¹⁴⁹	Arg¹⁵⁰	Gln¹⁵¹	Leu¹⁵²	Tyr¹⁵³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu¹⁵⁴	Asn¹⁵⁵	Lys¹⁵⁶	Pro¹⁵⁷	Arg¹⁵⁸	Arg¹⁵⁹	Pro¹⁶⁰	Tyr¹⁶¹	Ile¹⁶²	Leu¹⁶³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
EDILDAGNDKNEKEEVIKRKIPYILKRQLYENKPRRPYILKRGSYYY

Summary

#	organism	max score	hits	top seq
1	Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	99.00	2	NEUT_BOVIN Neurotensin/neuromedin N precursor
2	Bos taurus	99.00	1	neurotensin
3	Canis familiaris	96.70	1	PREDICTED: similar to Neurotensin/neuromedin N pr
4	Macaca fascicularis	94.40	1	hypothetical protein
5	Homo sapiens	92.40	2	neurotensin/neuromedin N preproprotein
6	Mus musculus	91.70	1	neurotensin
7	Rattus norvegicus	90.10	1	neurotensin
8	N/A	88.60	1	preproneurotensin/neuromedin N
9	Gallus gallus	59.70	1	PREDICTED: similar to Neurotensin/neuromedin N pr
10	dogs, ileum, Peptide, 20 aa	42.00	1	NT-TAIL=neurotensin/neuromedin N precursor derive

Top-ranked sequences

organism	matching
Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170
Bos taurus	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170
Canis familiaris	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 124 EDVLDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170
Macaca fascicularis	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 124 EDILDTGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170
Homo sapiens	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 124 EDILDTGNDKNGKEEVIKRKIPYILKRQLY#ENKPRRPYILKRDSYYY 170
Mus musculus	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 123 EDILDNVNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 169
Rattus norvegicus	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\| \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 123 EDILDHGNEKTEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRASYYY 169
N/A	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYYY 170 \|\|\|\|\| \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| Sbjct 79 EDILDHGNEKTEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRASYYY 125
Gallus gallus	Query 124 EDILDAGNDKNEKEEVIKRKIPYILKRQLY#ENKPRRPYILKRGSYY 169 +\| \| \| \|\|\| ++\|\|\| \|\|\|\|\|\|\|\|+# \|\| \|\|\|\|\|\|\|\| \|\|\| Sbjct 120 QDAFDLENSSQEKE-IMKRKNPYILKRQLH#VNKARRPYILKRSSYY 164
dogs, ileum, Peptide, 20 aa	Query 151 QLY#ENKPRRPYILKRGSYYY 170 +\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 ZLY#ENKPRRPYILKRGSYYY 20

[Site 2] KRQLYENKPR¹⁵⁸-RPYILKRGSY

Arg¹⁵⁸ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Lys¹⁴⁹	Arg¹⁵⁰	Gln¹⁵¹	Leu¹⁵²	Tyr¹⁵³	Glu¹⁵⁴	Asn¹⁵⁵	Lys¹⁵⁶	Pro¹⁵⁷	Arg¹⁵⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹⁵⁹	Pro¹⁶⁰	Tyr¹⁶¹	Ile¹⁶²	Leu¹⁶³	Lys¹⁶⁴	Arg¹⁶⁵	Gly¹⁶⁶	Ser¹⁶⁷	Tyr¹⁶⁸

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
AGNDKNEKEEVIKRKIPYILKRQLYENKPRRPYILKRGSYYY

Summary

#	organism	max score	hits	top seq
1	Canis familiaris	89.40	1	PREDICTED: similar to Neurotensin/neuromedin N pr
2	Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	89.00	2	NEUT_BOVIN Neurotensin/neuromedin N precursor
3	Bos taurus	89.00	1	neurotensin
4	Macaca fascicularis	84.70	1	hypothetical protein
5	Mus musculus	83.60	1	neurotensin
6	Homo sapiens	82.40	2	neurotensin/neuromedin N preproprotein
7	Rattus norvegicus	80.50	1	neurotensin
8	N/A	79.00	1	preproneurotensin/neuromedin N
9	Gallus gallus	57.40	1	PREDICTED: similar to Neurotensin/neuromedin N pr
10	dogs, ileum, Peptide, 20 aa	41.60	1	NT-TAIL=neurotensin/neuromedin N precursor derive

Top-ranked sequences

organism	matching
Canis familiaris	Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170
Contains: Large neuromedin N (NmN-125); Neuromedin N (NmN) (NN); Neurotensin (NT); Tail peptide	Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170
Bos taurus	Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170
Macaca fascicularis	Query 129 AGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 129 TGNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170
Mus musculus	Query 131 NDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Query 131 NDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170
Homo sapiens	Query 130 GNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 130 GNDKNGKEEVIKRKIPYILKRQLYENKPR#RPYILKRDSYYY 170
Rattus norvegicus	Query 130 GNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 129 GNEKTEKEEVIKRKIPYILKRQLYENKPR#RPYILKRASYYY 169
N/A	Query 130 GNDKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYYY 170 \|\|+\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\| \|\|\|\| Sbjct 85 GNEKTEKEEVIKRKIPYILKRQLYENKPR#RPYILKRASYYY 125
Gallus gallus	Query 132 DKNEKEEVIKRKIPYILKRQLYENKPR#RPYILKRGSYY 169 + ++++\|++\|\|\| \|\|\|\|\|\|\|\|+ \|\| \|#\|\|\|\|\|\|\| \|\|\| Sbjct 127 NSSQEKEIMKRKNPYILKRQLHVNKAR#RPYILKRSSYY 164
dogs, ileum, Peptide, 20 aa	Query 151 QLYENKPR#RPYILKRGSYYY 170 +\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 ZLYENKPR#RPYILKRGSYYY 20

* References

[PubMed ID: 19393038] Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marcais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL, A whole-genome assembly of the domestic cow, Bos taurus. Genome Biol. 2009;10(4):R42. doi: 10.1186/gb-2009-10-4-r42. Epub 2009 Apr 24.

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