CaMPDB :: Substrate

SB0035 : G-protein [gamma]-12 subunit

* Basic Information

Organism	Homo sapiens (human)
Protein Names	guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 precursor [Homo sapiens]; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 precursor; G-protein gamma-12 subunit; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12
Gene Names	GNG12; guanine nucleotide binding protein (G protein), gamma 12
Gene Locus	1p31.3; chromosome 1
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_061329	NM_018841	55970	Q9UBI6	615405	HGNC:19663	13592	hsa:55970

* Information From OMIM

Description: G proteins, which are involved in receptor-mediated cell signaling, are heterotrimers of alpha, beta, and gamma subunits. Alpha subunits confer receptor-dependent GTPase activity. The beta-gamma subcomplex is required for functional association of alpha subunits with receptors and exerts regulatory control over downstream effectors. Gamma subunits, such as GNG12, are thought to determine the functional specificity of the beta-gamma subcomplex (summary by Hurowitz et al., 2000).

* Structure Information

1. Primary Information

Length: 72 aa

Average Mass: 8.006 kDa

Monoisotopic Mass: 8.001 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 4 ---
CP12 domain 1.	5	46	20.0	0.1

3. Sequence Information

Fasta Sequence: SB0035.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 7493986] Morishita R, Nakayama H, Isobe T, Matsuda T, Hashimoto Y, Okano T, Fukada Y, Mizuno K, Ohno S, Kozawa O, et al., Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C. J Biol Chem. 1995 Dec 8;270(49):29469-75.

Cleavage sites (±10aa)

[Site 1] MSSK⁴-TASTNNIAQA

Lys⁴ Thr

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	-	-	-	-	Met¹	Ser²	Ser³	Lys⁴

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Thr⁵	Ala⁶	Ser⁷	Thr⁸	Asn⁹	Asn¹⁰	Ile¹¹	Ala¹²	Gln¹³	Ala¹⁴

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MSSKTASTNNIAQARRTVQQLRLEASIERIKVSK

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	66.60	1	G-protein gamma-12 subunit
2	Bos taurus	65.90	3	guanine nucleotide binding protein (G protein), g
3	Mus musculus	64.70	13	guanine nucleotide binding protein (G protein), g
4	Homo sapiens	63.90	6	Guanine nucleotide binding protein (G protein), g
5	Tetraodon nigroviridis	52.00	9	unnamed protein product
6	Rattus norvegicus	48.50	6	guanine nucleotide binding protein gamma 12 subun
7	Canis familiaris	48.50	2	PREDICTED: similar to Guanine nucleotide-binding
8	Xenopus tropicalis	48.10	1	guanine nucleotide binding protein (G protein), g
9	Xenopus laevis	47.40	1	guanine nucleotide binding protein (G protein), g
10	cattle, brain, Peptide, 55 aa	42.70	1	GTP binding regulatory protein gamma-II subunit,
11	Danio rerio	40.80	3	guanine nucleotide-binding protein gamma 2
12	Canis familiaris	40.80	3	PREDICTED: similar to Guanine nucleotide-binding
13	N/A	40.00	4	G Chain G, G Protein Heterotrimer Mutant Gi_alpha
14	Pongo pygmaeus	40.00	1	hypothetical protein
15	cattle, brain, Peptide, 67 aa	40.00	1	G protein gamma 2 subunit
16	synthetic construct	37.40	2	guanine nucleotide binding protein gamma 4

Top-ranked sequences

organism	matching
Homo sapiens	Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34
Bos taurus	Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSSK#TASTNNIAQARRTVQQLRMEASIERIKVSK 34
Mus musculus	Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \|\|\|\|#\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MSSK#TASTNSIAQARRTVQQLRLEASIERIKVSK 34
Homo sapiens	Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\| Sbjct 1 MSSK#TASTNNIAQARRTVQQLRLEASIEGIKVSK 34
Tetraodon nigroviridis	Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \|\|\|\|# \|+\|\|\| \|\|\|\|\|\|\|\|\|+\|\|\| +\|\|\|\|\|+ Sbjct 1 MSSK#AHSSNNINHARRTVQQLRVEASFQRIKVSR 34
Rattus norvegicus	Query 9 NNIAQARRTVQQLRLEASIERIKVSK 34 \|+ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 NSTAQARRTVQQLRLEASIERIKVSK 26
Canis familiaris	Query 1 MSSK#TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \|\|\|\|# \| ++\| \|\|\|\| \|+\|\|\|+\|\| \|+\|+\|\|\|\| Sbjct 1 MSSK#VAIGSDIGQARRAVEQLRMEAGIDRVKVSK 34
Xenopus tropicalis	Query 6 ASTNNIAQARRTVQQLRLEASIERIKVSK 34 +++\|\|\|\|\|\|\|+ \|+\|\|\|\|\|\| \|\|\|\|\|\|\|\| Sbjct 2 STSNNIAQARKLVEQLRLEAGIERIKVSK 30
Xenopus laevis	Query 6 ASTNNIAQARRTVQQLRLEASIERIKVSK 34 +++\|\|\|\|\|\|\|+ \|+\|\|\|\|\|\| \|\|\|+\|\|\|\| Sbjct 2 STSNNIAQARKLVEQLRLEAGIERVKVSK 30
cattle, brain, Peptide, 55 aa	Query 6 ASTNNIAQARRTVQQLRLEASIERIKVS 33 ++\|+\|\|\|\|\|\|+ \|+\|\|\|+\|\| \|\|\|\|\| \| Sbjct 1 SATDNIAQARKLVEQLRIEAGIERIKAS 28
Danio rerio	Query 5 TASTNNIAQARRTVQQLRLEASIERIKVSK 34 \| +\| +\|\|\|\|\|+ \|+\|\|++\|\|+\|+\|\|\|\|\|\| Sbjct 3 TNNTASIAQARKLVEQLKMEANIDRIKVSK 32
Canis familiaris	Query 7 STNNIAQARRTVQQLRLEASIERIKVSK 34 +\| +\|\|\|\|\|+ \|+\|\|++\|\|+\|+\|\|\|\|\|\| Sbjct 28 NTASIAQARKLVEQLKMEANIDRIKVSK 55
N/A	Query 7 STNNIAQARRTVQQLRLEASIERIKVSK 34 +\| +\|\|\|\|\|+ \|+\|\|++\|\|+\|+\|\|\|\|\|\| Sbjct 5 NTASIAQARKLVEQLKMEANIDRIKVSK 32
Pongo pygmaeus	Query 7 STNNIAQARRTVQQLRLEASIERIKVSK 34 +\| +\|\|\|\|\|+ \|+\|\|++\|\|+\|+\|\|\|\|\|\| Sbjct 5 NTASIAQARKLVEQLKMEANIDRIKVSK 32
cattle, brain, Peptide, 67 aa	Query 7 STNNIAQARRTVQQLRLEASIERIKVSK 34 +\| +\|\|\|\|\|+ \|+\|\|++\|\|+\|+\|\|\|\|\|\| Sbjct 4 NTASIAQARKLVEQLKMEANIDRIKVSK 31
synthetic construct	Query 7 STNNIAQARRTVQQLRLEASIERIKVSK 34 \|\| +\|+\|\|\|+ \|+\|\|++\|\| ++\|+\|\|\|+ Sbjct 9 STTSISQARKAVEQLKMEACMDRVKVSQ 36

* References

[PubMed ID: 19056867] Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA, Large-scale proteomics and phosphoproteomics of urinary exosomes. J Am Soc Nephrol. 2009 Feb;20(2):363-79. doi: 10.1681/ASN.2008040406. Epub 2008

» More (11) « Hide Below

[PubMed ID: 17185339] ... Fowler CE, Aryal P, Suen KF, Slesinger PA, Evidence for association of GABA(B) receptors with Kir3 channels and regulators of G protein signalling (RGS4) proteins. J Physiol. 2007 Apr 1;580(Pt 1):51-65. Epub 2006 Dec 21.

[PubMed ID: 17081983] ... Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M, Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48.

[PubMed ID: 15078334] ... Kato C, Mizutani T, Tamaki H, Kumagai H, Kamiya T, Hirobe A, Fujisawa Y, Kato H, Iwasaki Y, Characterization of heterotrimeric G protein complexes in rice plasma membrane. Plant J. 2004 Apr;38(2):320-31.

[PubMed ID: 12626323] ... Jiang G, Zhang BB, Glucagon and regulation of glucose metabolism. Am J Physiol Endocrinol Metab. 2003 Apr;284(4):E671-8.

[PubMed ID: 12486123] ... Cuello F, Schulze RA, Heemeyer F, Meyer HE, Lutz S, Jakobs KH, Niroomand F, Wieland T, Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Complex formation of NDPK B with Gbeta gamma dimers and phosphorylation of His-266 IN Gbeta. J Biol Chem. 2003 Feb 28;278(9):7220-6. Epub 2002 Dec 16.

[PubMed ID: 10931946] ... Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL, Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9543-8.

[PubMed ID: 10819326] ... Hurowitz EH, Melnyk JM, Chen YJ, Kouros-Mehr H, Simon MI, Shizuya H, Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes. DNA Res. 2000 Apr 28;7(2):111-20.

[PubMed ID: 10409705] ... Asano T, Morishita R, Ueda H, Kato K, Selective association of G protein beta(4) with gamma(5) and gamma(12) subunits in bovine tissues. J Biol Chem. 1999 Jul 23;274(30):21425-9.

[PubMed ID: 9003074] ... Morishita R, Saga S, Kawamura N, Hashizume Y, Inagaki T, Kato K, Asano T, Differential localization of the gamma 3 and gamma 12 subunits of G proteins in the mammalian brain. J Neurochem. 1997 Feb;68(2):820-7.

[PubMed ID: 7493986] ... Morishita R, Nakayama H, Isobe T, Matsuda T, Hashimoto Y, Okano T, Fukada Y, Mizuno K, Ohno S, Kozawa O, et al., Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C. J Biol Chem. 1995 Dec 8;270(49):29469-75.