SB0036 : [alpha]-Synuclein
[ CaMP Format ]
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | alpha-synuclein isoform NACP140 [Homo sapiens]; alpha-synuclein isoform NACP140; non A-beta component of AD amyloid; synuclein alpha-140; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP |
| Gene Names | SNCA; NACP, PARK1; NACP; PARK1; synuclein, alpha (non A4 component of amyloid precursor) |
| Gene Locus | 4q21; chromosome 4 |
| GO Function | Not available |
* Information From OMIM
Description: Alpha-synuclein is a highly conserved protein that is abundant in neurons, especially presynaptic terminals. Aggregated alpha-synuclein proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies (summary by Giasson et al., 2000).
Function: Jakes et al. (1994) used immunohistochemistry to show that alpha-synuclein is concentrated in presynaptic nerve terminals.
* Structure Information
1. Primary Information
Length: 140 aa
Average Mass: 14.460 kDa
Monoisotopic Mass: 14.451 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| Synuclein 1. | 1 | 130 | 1.0 | 17.6 |
| --- cleavage 57 (inside Synuclein 1..130) --- | ||||
| --- cleavage 73 (inside Synuclein 1..130) --- | ||||
| --- cleavage 74 (inside Synuclein 1..130) --- | ||||
| --- cleavage 83 (inside Synuclein 1..130) --- | ||||
3. Sequence Information
Fasta Sequence: SB0036.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB: 1XQ8 (NMR; -; A=1-140), 2JN5 (NMR; -; A=1-12), 2KKW (NMR; -; A=1-140), 2M55 (NMR; -; B=1-19), 2X6M (X-ray; 162 A; B=132-140), 3Q25 (X-ray; 190 A; A=1-19), 3Q26 (X-ray; 154 A; A=10-42), 3Q27 (X-ray; 130 A; A=32-57), 3Q28 (X-ray; 160 A; A=58-79), 3Q29 (X-ray; 230 A; A/C=1-19), 4BXL (NMR; -; C=35-56), 4R0U (X-ray; 138 A; A=72-78), 4R0W (X-ray; 150 A; A=70-76)
* Cleavage Information
4 [sites] cleaved by Calpain 1
Source Reference: [PubMed ID: 15909996] Mishizen-Eberz AJ, Norris EH, Giasson BI, Hodara R, Ischiropoulos H, Lee VM, Trojanowski JQ, Lynch DR, Cleavage of alpha-synuclein by calpain: potential role in degradation of fibrillized and nitrated species of alpha-synuclein. Biochemistry. 2005 May 31;44(21):7818-29.
Cleavage sites (±10aa)
[Site 1] VVHGVATVAE57-KTKEQVTNVG
Glu57 
Lys
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val48 | Val49 | His50 | Gly51 | Val52 | Ala53 | Thr54 | Val55 | Ala56 | Glu57 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Lys58 | Thr59 | Lys60 | Glu61 | Gln62 | Val63 | Thr64 | Asn65 | Val66 | Gly67 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| EAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGS |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 109.00 | 7 | NACP/alpha-synuclein |
| 2 | synthetic construct | 109.00 | 4 | Homo sapiens synuclein, alpha (non A4 component o |
| 3 | Homo sapiens | 109.00 | 2 | alpha-synuclein isoform NACP140 |
| 4 | Erythrocebus patas | 109.00 | 1 | SYUA_ERYPA Alpha-synuclein gi |
| 5 | Macaca fascicularis | 109.00 | 1 | SYUA_MACFA Alpha-synuclein gi |
| 6 | Pongo abelii | 108.00 | 1 | SYUA_PONPA Alpha-synuclein gi |
| 7 | Rattus sp. | 106.00 | 3 | synuclein SYN2 |
| 8 | Mus musculus | 105.00 | 6 | synuclein, alpha |
| 9 | Rattus norvegicus | 105.00 | 2 | synuclein, alpha |
| 10 | Saguinus labiatus | 105.00 | 1 | SYUA_SAGLB Alpha-synuclein gi |
| 11 | Canis familiaris | 104.00 | 4 | PREDICTED: similar to alpha-synuclein isoform NAC |
| 12 | Gallus gallus | 104.00 | 3 | synuclein, alpha (non A4 component of amyloid pre |
| 13 | N/A | 104.00 | 2 | SYUA_SERCA Alpha-synuclein (Synelfin) gi |
| 14 | Taeniopygia guttata | 104.00 | 1 | synuclein, alpha (non A4 component of amyloid pre |
| 15 | Sus scrofa | 104.00 | 1 | alpha-synuclein |
| 16 | Xenopus laevis | 103.00 | 4 | synuclein, alpha |
| 17 | Bos taurus | 103.00 | 2 | synuclein, alpha (non A4 component of amyloid pre |
| 18 | Lagothrix lagotricha | 103.00 | 1 | SYUA_ATEGE Alpha-synuclein gi |
| 19 | Pan troglodytes | 75.50 | 1 | PREDICTED: alpha-synuclein isoform 3 |
| 20 | cattle, brain, Peptide, 134 aa | 72.00 | 1 | SYUB_BOVIN Beta-synuclein (Phosphoneuroprotein 14 |
| 21 | Xenopus tropicalis | 70.50 | 2 | synuclein, beta |
| 22 | Takifugu rubripes | 61.20 | 4 | gamma1-synuclein |
| 23 | Tetraodon nigroviridis | 58.90 | 3 | unnamed protein product |
| 24 | Danio rerio | 57.80 | 2 | Zgc:123342 protein |
| 25 | Danio rerio | 35.00 | 1 | hypothetical protein LOC393944 |
| 26 | Macaca mulatta | 30.00 | 1 | PREDICTED: similar to synuclein, gamma (breast ca |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |
| synthetic construct | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |
| Homo sapiens | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |
| Erythrocebus patas | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |
| Macaca fascicularis | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |
| Pongo abelii | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |
| Rattus sp. | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGN 87 |
| Mus musculus | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGN 87 |
| Rattus norvegicus | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGN 87 |
| Saguinus labiatus | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGN 87 |
| Canis familiaris | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||||| ||||||||||||||||||| Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGEAVVTGVTAVAQKTVEGAGS 87 |
| Gallus gallus | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |||||||||||||||+||||||||| ||||#||||||+||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSRTKEGVVHGVTTVAE#KTKEQVSNVGGAVVTGVTAVAQKTVEGAGN 87 |
| N/A | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |||||||||||||||+||||||||| ||||#||||||+||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSRTKEGVVHGVTTVAE#KTKEQVSNVGGAVVTGVTAVAQKTVEGAGN 87 |
| Taeniopygia guttata | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 |||||||||||||||+||||||||| ||||#||||||+||||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSRTKEGVVHGVTTVAE#KTKEQVSNVGGAVVTGVTAVAQKTVEGAGN 87 |
| Sus scrofa | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||||| ||||||||||||||||||| Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGEAVVTGVTAVAQKTVEGAGS 87 |
| Xenopus laevis | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#||||||+|||||||||||||| |||||||+ Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVSNVGGAVVTGVTAVAHKTVEGAGN 87 |
| Bos taurus | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||+|||||||||||||||||||| ||||#|||||||||| ||||||||||||||||||| Sbjct 28 EAAGRTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTNVGEAVVTGVTAVAQKTVEGAGS 87 |
| Lagothrix lagotricha | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 ||||||||||||||||||||||||| ||||#|||||||+|||||||||||||||||||||+ Sbjct 28 EAAGKTKEGVLYVGSKTKEGVVHGVTTVAE#KTKEQVTSVGGAVVTGVTAVAQKTVEGAGN 87 |
| Pan troglodytes | Query 29 AAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 87 || |||+|| ||||||++ |||#|||||||||||||||||||||||||||||| Sbjct 18 AAEKTKQGVAEAAGKTKEGVLY---VVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGAGS 73 |
| cattle, brain, Peptide, 134 aa | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKT 81 ||| |||||||||||||||||| |||+|||#||||| +++|||| +| +| | Sbjct 28 EAAEKTKEGVLYVGSKTKEGVVQGVASVAE#KTKEQASHLGGAVFSGAGNIAAAT 81 |
| Xenopus tropicalis | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKT 81 ||| |||||||||||||++||| |||+|||#||||| + +||||++| +| | Sbjct 28 EAAEKTKEGVLYVGSKTRDGVVQGVASVAE#KTKEQASQLGGAVMSGAGNIAAAT 81 |
| Takifugu rubripes | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEG 84 ||| ||||||+|||+|| |||| | || #|| +| || ||| | |+| |||| Sbjct 28 EAAAKTKEGVMYVGNKTMEGVVSSVNTVTN#KTVDQTNIVGDAVVGGANEVSQATVEG 84 |
| Tetraodon nigroviridis | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEG 84 ||| ||||||+|||+|| |||| || ||| #|| || + | | ||| ||| Sbjct 28 EAATKTKEGVIYVGNKTMEGVVTGVNTVAH#KTTEQANIIADTAVAGANEVAQSAVEG 84 |
| Danio rerio | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEG 84 ||| ||||||+|||+|||||| | |||+#|| +| + | | |++| ||| Sbjct 28 EAAAKTKEGVMYVGTKTKEGVATSVNTVAQ#KTTDQANLMSDTAVAGANEVSEKAVEG 84 |
| Danio rerio | Query 29 AAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVA 78 || ||||||++||||||+ | |||||#||||| + +||||++| +| Sbjct 29 AAEKTKEGVMFVGSKTKDSV----ATVAE#KTKEQASQLGGAVMSGAGNIA 74 Query 32 KTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGA 85 | |||+ |||||| | ||#|||| | || | ||+|| | | Sbjct 10 KAKEGMAVAAEKTKEGV----AVAAE#KTKEGVMFVGSKTKDSVATVAEKTKEQA 59 |
| Macaca mulatta | Query 28 EAAGKTKEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGAVVTGVTAVAQKTVEGA 85 ||| ||||||+|||+|||| ||| | +|||#||||| | |||+ | || |||| | Sbjct 28 EAAEKTKEGVMYVGTKTKENVVHSVTSVAE#KTKEQANAVSEAVVSSVNTVAAKTVEEA 85 Query 34 KEGVLYVGSKTKEGVVHGVATVAE#KTKEQVTNVGGA----VVTGVTAVAQKTVEGAGS 87 ||||+ |||+|| ||#|||| | || || ||+||+|| | | + Sbjct 12 KEGVVGAVEKTKQGVTEA----AE#KTKEGVMYVGTKTKENVVHSVTSVAEKTKEQANA 65 |
[Site 2] TNVGGAVVTG73-VTAVAQKTVE
Gly73 Val
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Thr64 | Asn65 | Val66 | Gly67 | Gly68 | Ala69 | Val70 | Val71 | Thr72 | Gly73 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Val74 | Thr75 | Ala76 | Val77 | Ala78 | Gln79 | Lys80 | Thr81 | Val82 | Glu83 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| TKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKN |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | synthetic construct | 112.00 | 4 | Homo sapiens synuclein, alpha (non A4 component o |
| 2 | Homo sapiens | 112.00 | 2 | alpha-synuclein isoform NACP140 |
| 3 | Pongo abelii | 112.00 | 1 | SYUA_PONPA Alpha-synuclein gi |
| 4 | Erythrocebus patas | 112.00 | 1 | SYUA_ERYPA Alpha-synuclein gi |
| 5 | Macaca fascicularis | 111.00 | 1 | SYUA_MACFA Alpha-synuclein gi |
| 6 | Homo sapiens | 110.00 | 7 | NACP/alpha-synuclein |
| 7 | Mus musculus | 105.00 | 5 | synuclein, alpha |
| 8 | Rattus sp. | 105.00 | 2 | synuclein SYN2 |
| 9 | Rattus norvegicus | 105.00 | 2 | synuclein, alpha |
| 10 | Sus scrofa | 104.00 | 1 | alpha-synuclein |
| 11 | Saguinus labiatus | 103.00 | 1 | SYUA_SAGLB Alpha-synuclein gi |
| 12 | Canis familiaris | 101.00 | 4 | PREDICTED: similar to alpha-synuclein isoform NAC |
| 13 | Gallus gallus | 100.00 | 3 | synuclein, alpha (non A4 component of amyloid pre |
| 14 | N/A | 100.00 | 2 | SYUA_SERCA Alpha-synuclein (Synelfin) gi |
| 15 | Taeniopygia guttata | 100.00 | 1 | synuclein, alpha (non A4 component of amyloid pre |
| 16 | Lagothrix lagotricha | 99.40 | 1 | SYUA_ATEGE Alpha-synuclein gi |
| 17 | Bos taurus | 98.60 | 2 | synuclein, alpha (non A4 component of amyloid pre |
| 18 | Pan troglodytes | 97.40 | 1 | PREDICTED: alpha-synuclein isoform 3 |
| 19 | Xenopus laevis | 93.20 | 4 | synuclein, alpha |
| 20 | Takifugu rubripes | 60.80 | 4 | alpha-synuclein |
| 21 | Xenopus tropicalis | 58.50 | 2 | synuclein, beta |
| 22 | cattle, brain, Peptide, 134 aa | 57.80 | 1 | SYUB_BOVIN Beta-synuclein (Phosphoneuroprotein 14 |
| 23 | Danio rerio | 52.00 | 1 | hypothetical protein LOC393944 |
| 24 | Tetraodon nigroviridis | 43.90 | 3 | unnamed protein product |
| 25 | Danio rerio | 38.10 | 2 | hypothetical protein LOC550229 |
| 26 | Macaca mulatta | 30.40 | 1 | PREDICTED: similar to synuclein, gamma (breast ca |
Top-ranked sequences
| organism | matching |
|---|---|
| synthetic construct | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 |
| Homo sapiens | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 |
| Pongo abelii | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 |
| Erythrocebus patas | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 |
| Macaca fascicularis | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||||||||||||||||||||||||#|||||||||||||||||||||+|||||||| Sbjct 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFIKKDQLGKN 103 |
| Homo sapiens | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||||||||||||||||||||||||#||||||||||||||||| |||||||||||| Sbjct 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAXTGFVKKDQLGKN 103 |
| Mus musculus | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| ||||||||||||||||||||#|||||||||||||+||||||||||||+|| Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGNIAAATGFVKKDQMGK 102 |
| Rattus sp. | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| ||||||||||||||||||||#|||||||||||||+||||||||||||+|| Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGNIAAATGFVKKDQMGK 102 |
| Rattus norvegicus | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| ||||||||||||||||||||#|||||||||||||+||||||||||||+|| Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGNIAAATGFVKKDQMGK 102 |
| Sus scrofa | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||| |||||||||||||| |||||#||||||||||||||||||||| |||||||| Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGEAVVTG#VTAVAQKTVEGAGSIAAATGFGKKDQLGKN 103 |
| Saguinus labiatus | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||| ||||||||||||||||||||#|||||||||||||+||||||||+|| |||+ Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGNIAAATGFVRKDHLGKS 103 |
| Canis familiaris | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||| |||||||||||||| |||||#||||||||||||||||||||| |||||||+ Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGEAVVTG#VTAVAQKTVEGAGSIAAATGFGKKDQLGKS 103 |
| Gallus gallus | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| ||||||||||+|||||||||#|||||||||||||+|||||| |||||| | Sbjct 44 TKEGVVHGVTTVAEKTKEQVSNVGGAVVTG#VTAVAQKTVEGAGNIAAATGLVKKDQLAK 102 |
| N/A | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| ||||||||||+|||||||||#|||||||||||||+|||||| |||||| | Sbjct 44 TKEGVVHGVTTVAEKTKEQVSNVGGAVVTG#VTAVAQKTVEGAGNIAAATGLVKKDQLAK 102 |
| Taeniopygia guttata | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| ||||||||||+|||||||||#|||||||||||||+|||||| |||||| | Sbjct 44 TKEGVVHGVTTVAEKTKEQVSNVGGAVVTG#VTAVAQKTVEGAGNIAAATGLVKKDQLAK 102 |
| Lagothrix lagotricha | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 ||||||||| |||||||||||+||||||||#|||||||||||||+||||||||||| ||+ Sbjct 44 TKEGVVHGVTTVAEKTKEQVTSVGGAVVTG#VTAVAQKTVEGAGNIAAATGFVKKDHSGKS 103 |
| Bos taurus | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGK 102 ||||||||| |||||||||||||| |||||#||||||||||||||||||||| ||| +|| Sbjct 44 TKEGVVHGVTTVAEKTKEQVTNVGEAVVTG#VTAVAQKTVEGAGSIAAATGFGKKDHMGK 102 |
| Pan troglodytes | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 103 |||||++ |||||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 33 TKEGVLY---VVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQLGKN 89 |
| Xenopus laevis | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQ 99 ||||||||| ||||||||||+|||||||||#||||| |||||||+ ||||| ||||| Sbjct 44 TKEGVVHGVTTVAEKTKEQVSNVGGAVVTG#VTAVAHKTVEGAGNFAAATGLVKKDQ 99 |
| Takifugu rubripes | Query 44 TKEGVV-------HGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVK 96 ||+||+ || || || |+ ||||+|||#|||||||||| ||||||||| || Sbjct 33 TKDGVMFVGTKTKDGVTVVAGKTVSGVSQVGGAMVTG#VTAVAQKTVESAGSIAAATGLVK 92 Query 97 KD 98 |+ Sbjct 93 KE 94 |
| Xenopus tropicalis | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQL 100 |++||| |||+|||||||| + +||||++ # |||+|||||| ||||+ Sbjct 44 TRDGVVQGVASVAEKTKEQASQLGGAVMS-#----------GAGNIAAATGLVKKDEF 89 |
| cattle, brain, Peptide, 134 aa | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQL 100 |||||| |||+|||||||| +++|||| + # |||+|||||| |||++ Sbjct 44 TKEGVVQGVASVAEKTKEQASHLGGAVFS-#----------GAGNIAAATGLVKKEEF 89 |
| Danio rerio | Query 44 TKEGVV-------HGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVK 96 |||||+ ||||||||||| + +||||++|# ||+|||||| || Sbjct 33 TKEGVMFVGSKTKDSVATVAEKTKEQASQLGGAVMSG#-----------AGNIAAATGLVK 81 Query 97 KDQLGKN 103 ||+ + Sbjct 82 KDEFPSD 88 |
| Tetraodon nigroviridis | Query 46 EGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKK 97 |||| | ||| +| +| || ||| |# |+| |||| ++||++| + + Sbjct 2 EGVVSSVNTVANRTVDQANIVGDAVVGG#ANEVSQATVEGVENVAASSGLISQ 53 |
| Danio rerio | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVK 96 ||||| | |||+|| +| + | |# |++| ||| ++ |+ | || Sbjct 44 TKEGVATSVNTVAQKTTDQANLMSDTAVAG#ANEVSEKAVEGLETMVASAGLVK 96 |
| Macaca mulatta | Query 44 TKEGVVHGVATVAEKTKEQVTNVGGAVVTG#VTAVAQKTVEGAGSIAAATGFVKKDQL 100 ||| ||| | +|||||||| | |||+ #| || |||| | +|| +| |+|+ | Sbjct 44 TKENVVHSVTSVAEKTKEQANAVSEAVVSS#VNTVAAKTVEEAENIAVTSGVVRKEDL 100 Query 45 KEGVV-------HGVATVAEKTKEQVTNVGGA----VVTG#VTAVAQKTVEGAGSIAAA 91 ||||| || |||||| | || || #||+||+|| | | +++ | Sbjct 12 KEGVVGAVEKTKQGVTEAAEKTKEGVMYVGTKTKENVVHS#VTSVAEKTKEQANAVSEA 69 |
[Site 3] NVGGAVVTGV74-TAVAQKTVEG
Val74 Thr
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Asn65 | Val66 | Gly67 | Gly68 | Ala69 | Val70 | Val71 | Thr72 | Gly73 | Val74 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Thr75 | Ala76 | Val77 | Ala78 | Gln79 | Lys80 | Thr81 | Val82 | Glu83 | Gly84 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| KEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNE |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | synthetic construct | 112.00 | 4 | Homo sapiens synuclein, alpha (non A4 component o |
| 2 | Homo sapiens | 112.00 | 2 | alpha-synuclein isoform NACP140 |
| 3 | Erythrocebus patas | 112.00 | 1 | SYUA_ERYPA Alpha-synuclein gi |
| 4 | Pongo abelii | 112.00 | 1 | SYUA_PONPA Alpha-synuclein gi |
| 5 | Macaca fascicularis | 111.00 | 1 | SYUA_MACFA Alpha-synuclein gi |
| 6 | Homo sapiens | 110.00 | 7 | NACP/alpha-synuclein |
| 7 | Rattus norvegicus | 105.00 | 2 | synuclein, alpha |
| 8 | Mus musculus | 104.00 | 5 | synuclein, alpha |
| 9 | Sus scrofa | 104.00 | 1 | alpha-synuclein |
| 10 | Rattus sp. | 103.00 | 2 | synuclein SYN2 |
| 11 | Saguinus labiatus | 103.00 | 1 | SYUA_SAGLB Alpha-synuclein gi |
| 12 | Canis familiaris | 102.00 | 4 | PREDICTED: similar to alpha-synuclein isoform NAC |
| 13 | Lagothrix lagotricha | 99.40 | 1 | SYUA_ATEGE Alpha-synuclein gi |
| 14 | Gallus gallus | 98.60 | 3 | synuclein, alpha (non A4 component of amyloid pre |
| 15 | N/A | 98.60 | 2 | SYUA_SERCA Alpha-synuclein (Synelfin) gi |
| 16 | Bos taurus | 98.60 | 2 | synuclein, alpha (non A4 component of amyloid pre |
| 17 | Taeniopygia guttata | 98.60 | 1 | synuclein, alpha (non A4 component of amyloid pre |
| 18 | Pan troglodytes | 97.40 | 1 | PREDICTED: alpha-synuclein isoform 3 |
| 19 | Xenopus laevis | 92.80 | 4 | synuclein, alpha |
| 20 | Takifugu rubripes | 60.10 | 4 | alpha-synuclein |
| 21 | Xenopus tropicalis | 57.00 | 2 | synuclein, beta |
| 22 | cattle, brain, Peptide, 134 aa | 56.20 | 1 | SYUB_BOVIN Beta-synuclein (Phosphoneuroprotein 14 |
| 23 | Danio rerio | 50.10 | 1 | hypothetical protein LOC393944 |
| 24 | Tetraodon nigroviridis | 43.90 | 3 | unnamed protein product |
| 25 | Danio rerio | 36.60 | 2 | hypothetical protein LOC550229 |
| 26 | Macaca mulatta | 30.00 | 1 | PREDICTED: similar to synuclein, gamma (breast ca |
Top-ranked sequences
| organism | matching |
|---|---|
| synthetic construct | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |
| Homo sapiens | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |
| Erythrocebus patas | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |
| Pongo abelii | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |
| Macaca fascicularis | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||||||||||||||||||||||||||||#||||||||||||||||||||+||||||||| Sbjct 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFIKKDQLGKNE 104 |
| Homo sapiens | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||||||||||||||||||||||||||||#|||||||||||||||| ||||||||||||| Sbjct 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAXTGFVKKDQLGKNE 104 |
| Rattus norvegicus | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||||||||||||#||||||||||||+||||||||||||+|| | Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGNIAAATGFVKKDQMGKGE 104 |
| Mus musculus | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||||||||||||#||||||||||||+||||||||||||+|| | Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGNIAAATGFVKKDQMGKGE 104 |
| Sus scrofa | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||||| ||||||#|||||||||||||||||||| ||||||||| Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGEAVVTGV#TAVAQKTVEGAGSIAAATGFGKKDQLGKNE 104 |
| Rattus sp. | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGK 102 |||||||| |||||||||||||||||||||#||||||||||||+||||||||||||+|| Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGNIAAATGFVKKDQMGK 102 |
| Saguinus labiatus | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||||||||||||#||||||||||||+||||||||+|| |||+| Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGNIAAATGFVRKDHLGKSE 104 |
| Canis familiaris | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||||| ||||||#|||||||||||||||||||| |||||||+| Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGEAVVTGV#TAVAQKTVEGAGSIAAATGFGKKDQLGKSE 104 |
| Lagothrix lagotricha | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||+|||||||||#||||||||||||+||||||||||| ||+| Sbjct 45 KEGVVHGVTTVAEKTKEQVTSVGGAVVTGV#TAVAQKTVEGAGNIAAATGFVKKDHSGKSE 104 |
| Gallus gallus | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGK 102 |||||||| ||||||||||+||||||||||#||||||||||||+|||||| |||||| | Sbjct 45 KEGVVHGVTTVAEKTKEQVSNVGGAVVTGV#TAVAQKTVEGAGNIAAATGLVKKDQLAK 102 |
| N/A | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGK 102 |||||||| ||||||||||+||||||||||#||||||||||||+|||||| |||||| | Sbjct 45 KEGVVHGVTTVAEKTKEQVSNVGGAVVTGV#TAVAQKTVEGAGNIAAATGLVKKDQLAK 102 |
| Bos taurus | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| |||||||||||||| ||||||#|||||||||||||||||||| ||| +|| | Sbjct 45 KEGVVHGVTTVAEKTKEQVTNVGEAVVTGV#TAVAQKTVEGAGSIAAATGFGKKDHMGKGE 104 |
| Taeniopygia guttata | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGK 102 |||||||| ||||||||||+||||||||||#||||||||||||+|||||| |||||| | Sbjct 45 KEGVVHGVTTVAEKTKEQVSNVGGAVVTGV#TAVAQKTVEGAGNIAAATGLVKKDQLAK 102 |
| Pan troglodytes | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 ||||++ ||||||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 34 KEGVLY---VVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 90 |
| Xenopus laevis | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQLGKNE 104 |||||||| ||||||||||+||||||||||#|||| |||||||+ ||||| ||||| ||| Sbjct 45 KEGVVHGVTTVAEKTKEQVSNVGGAVVTGV#TAVAHKTVEGAGNFAAATGLVKKDQ--KNE 102 |
| Takifugu rubripes | Query 50 HGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKD 98 || || || |+ ||||+||||#||||||||| ||||||||| |||+ Sbjct 46 DGVTVVAGKTVSGVSQVGGAMVTGV#TAVAQKTVESAGSIAAATGLVKKE 94 |
| Xenopus tropicalis | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQL 100 ++||| |||+|||||||| + +||||++ # |||+|||||| ||||+ Sbjct 45 RDGVVQGVASVAEKTKEQASQLGGAVMS--#---------GAGNIAAATGLVKKDEF 89 |
| cattle, brain, Peptide, 134 aa | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQL 100 ||||| |||+|||||||| +++|||| + # |||+|||||| |||++ Sbjct 45 KEGVVQGVASVAEKTKEQASHLGGAVFS--#---------GAGNIAAATGLVKKEEF 89 |
| Danio rerio | Query 45 KEGVV-------HGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKK 97 ||||+ ||||||||||| + +||||++| # ||+|||||| ||| Sbjct 34 KEGVMFVGSKTKDSVATVAEKTKEQASQLGGAVMSG-#----------AGNIAAATGLVKK 82 Query 98 DQLGKN 103 |+ + Sbjct 83 DEFPSD 88 |
| Tetraodon nigroviridis | Query 46 EGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKK 97 |||| | ||| +| +| || ||| | # |+| |||| ++||++| + + Sbjct 2 EGVVSSVNTVANRTVDQANIVGDAVVGGA#NEVSQATVEGVENVAASSGLISQ 53 |
| Danio rerio | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVK 96 |||| | |||+|| +| + | | # |++| ||| ++ |+ | || Sbjct 45 KEGVATSVNTVAQKTTDQANLMSDTAVAGA#NEVSEKAVEGLETMVASAGLVK 96 |
| Macaca mulatta | Query 45 KEGVVHGVATVAEKTKEQVTNVGGAVVTGV#TAVAQKTVEGAGSIAAATGFVKKDQL 100 || ||| | +|||||||| | |||+ |# || |||| | +|| +| |+|+ | Sbjct 45 KENVVHSVTSVAEKTKEQANAVSEAVVSSV#NTVAAKTVEEAENIAVTSGVVRKEDL 100 Query 45 KEGVV-------HGVATVAEKTKEQVTNVGGA----VVTGV#TAVAQKTVEGAGSIAAA 91 ||||| || |||||| | || || |#|+||+|| | | +++ | Sbjct 12 KEGVVGAVEKTKQGVTEAAEKTKEGVMYVGTKTKENVVHSV#TSVAEKTKEQANAVSEA 69 |
[Site 4] VTAVAQKTVE83-GAGSIAAATG
Glu83 Gly
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val74 | Thr75 | Ala76 | Val77 | Ala78 | Gln79 | Lys80 | Thr81 | Val82 | Glu83 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gly84 | Ala85 | Gly86 | Ser87 | Ile88 | Ala89 | Ala90 | Ala91 | Thr92 | Gly93 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| TVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGIL |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | synthetic construct | 113.00 | 4 | Homo sapiens synuclein, alpha (non A4 component o |
| 2 | Homo sapiens | 113.00 | 2 | alpha-synuclein isoform NACP140 |
| 3 | Erythrocebus patas | 113.00 | 1 | SYUA_ERYPA Alpha-synuclein gi |
| 4 | Macaca fascicularis | 113.00 | 1 | SYUA_MACFA Alpha-synuclein gi |
| 5 | Homo sapiens | 111.00 | 7 | NACP/alpha-synuclein |
| 6 | Pan troglodytes | 111.00 | 1 | PREDICTED: alpha-synuclein isoform 3 |
| 7 | Pongo abelii | 109.00 | 1 | SYUA_PONPA Alpha-synuclein gi |
| 8 | Sus scrofa | 107.00 | 1 | alpha-synuclein |
| 9 | Saguinus labiatus | 107.00 | 1 | SYUA_SAGLB Alpha-synuclein gi |
| 10 | Mus musculus | 106.00 | 5 | synuclein, alpha |
| 11 | Rattus norvegicus | 106.00 | 2 | synuclein, alpha |
| 12 | Canis familiaris | 103.00 | 4 | PREDICTED: similar to alpha-synuclein isoform NAC |
| 13 | Lagothrix lagotricha | 103.00 | 1 | SYUA_ATEGE Alpha-synuclein gi |
| 14 | Bos taurus | 98.60 | 2 | synuclein, alpha (non A4 component of amyloid pre |
| 15 | Gallus gallus | 92.00 | 3 | synuclein, alpha (non A4 component of amyloid pre |
| 16 | N/A | 92.00 | 2 | SYUA_SERCA Alpha-synuclein (Synelfin) gi |
| 17 | Taeniopygia guttata | 92.00 | 1 | synuclein, alpha (non A4 component of amyloid pre |
| 18 | Rattus sp. | 89.00 | 2 | synuclein SYN2 |
| 19 | Xenopus laevis | 81.60 | 4 | synuclein, alpha |
| 20 | Takifugu rubripes | 58.50 | 4 | alpha-synuclein |
| 21 | Danio rerio | 45.80 | 1 | hypothetical protein LOC393944 |
| 22 | Macaca mulatta | 45.40 | 1 | PREDICTED: similar to synuclein, gamma (breast ca |
| 23 | Xenopus tropicalis | 41.60 | 2 | synuclein, beta |
| 24 | cattle, brain, Peptide, 134 aa | 39.70 | 1 | SYUB_BOVIN Beta-synuclein (Phosphoneuroprotein 14 |
| 25 | Tetraodon nigroviridis | 37.00 | 4 | unnamed protein product |
| 26 | Danio rerio | 30.00 | 1 | hypothetical protein LOC550229 |
Top-ranked sequences
| organism | matching |
|---|---|
| synthetic construct | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |
| Homo sapiens | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |
| Erythrocebus patas | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |
| Macaca fascicularis | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||||||||||+|||||||||||||||||| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFIKKDQLGKNEEGAPQEGIL 113 |
| Homo sapiens | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#||||||| |||||||||||||||||||||| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAXTGFVKKDQLGKNEEGAPQEGIL 113 |
| Pan troglodytes | Query 55 VAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 55 VAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |
| Pongo abelii | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||| ||||| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGATQEGIL 113 |
| Sus scrofa | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |||||||||||||| |||||||||||||||#||||||||||| |||||||||||||||||| Sbjct 54 TVAEKTKEQVTNVGEAVVTGVTAVAQKTVE#GAGSIAAATGFGKKDQLGKNEEGAPQEGIL 113 |
| Saguinus labiatus | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||+||||||||+|| |||+|||||||||| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGNIAAATGFVRKDHLGKSEEGAPQEGIL 113 |
| Mus musculus | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||+||||||||||||+|| ||| |||||| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGNIAAATGFVKKDQMGKGEEGYPQEGIL 113 |
| Rattus norvegicus | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||||||||||||||||||||||#|||+||||||||||||+|| ||| |||||| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGNIAAATGFVKKDQMGKGEEGYPQEGIL 113 |
| Canis familiaris | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |||||||||||||| |||||||||||||||#||||||||||| |||||||+||| |||||| Sbjct 54 TVAEKTKEQVTNVGEAVVTGVTAVAQKTVE#GAGSIAAATGFGKKDQLGKSEEGGPQEGIL 113 |
| Lagothrix lagotricha | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |||||||||||+||||||||||||||||||#|||+||||||||||| ||+|||||||||| Sbjct 54 TVAEKTKEQVTSVGGAVVTGVTAVAQKTVE#GAGNIAAATGFVKKDHSGKSEEGAPQEGIL 113 |
| Bos taurus | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 |||||||||||||| |||||||||||||||#||||||||||| ||| +|| |||| ||||| Sbjct 54 TVAEKTKEQVTNVGEAVVTGVTAVAQKTVE#GAGSIAAATGFGKKDHMGKGEEGASQEGIL 113 |
| Gallus gallus | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGK-NEEGAPQEGI 112 ||||||||||+|||||||||||||||||||#|||+|||||| |||||| | |||| |||+ Sbjct 54 TVAEKTKEQVSNVGGAVVTGVTAVAQKTVE#GAGNIAAATGLVKKDQLAKQNEEGFLQEGM 113 Query 113 L 113 + Sbjct 114 V 114 |
| N/A | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGK-NEEGAPQEGI 112 ||||||||||+|||||||||||||||||||#|||+|||||| |||||| | |||| |||+ Sbjct 54 TVAEKTKEQVSNVGGAVVTGVTAVAQKTVE#GAGNIAAATGLVKKDQLAKQNEEGFLQEGM 113 Query 113 L 113 + Sbjct 114 V 114 |
| Taeniopygia guttata | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGK-NEEGAPQEGI 112 ||||||||||+|||||||||||||||||||#|||+|||||| |||||| | |||| |||+ Sbjct 54 TVAEKTKEQVSNVGGAVVTGVTAVAQKTVE#GAGNIAAATGLVKKDQLAKQNEEGFLQEGM 113 Query 113 L 113 + Sbjct 114 V 114 |
| Rattus sp. | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGK 102 ||||||||||||||||||||||||||||||#|||+||||||||||||+|| Sbjct 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGNIAAATGFVKKDQMGK 102 |
| Xenopus laevis | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGIL 113 ||||||||||+|||||||||||||| ||||#|||+ ||||| ||||| ||| | || + Sbjct 54 TVAEKTKEQVSNVGGAVVTGVTAVAHKTVE#GAGNFAAATGLVKKDQ--KNESGFGPEGTM 111 |
| Takifugu rubripes | Query 55 VAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQEGI 112 || || |+ ||||+|||||||||||||# ||||||||| |||+ + ++ | | + Sbjct 51 VAGKTVSGVSQVGGAMVTGVTAVAQKTVE#SAGSIAAATGLVKKEPGKQGDDAAAPENM 108 |
| Danio rerio | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQL 100 ||||||||| + +||||++| # ||+|||||| ||||+ Sbjct 50 TVAEKTKEQASQLGGAVMSG----------#-AGNIAAATGLVKKDEF 85 |
| Macaca mulatta | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKKDQLGKNEEGAPQE 110 +|||||||| | |||+ | || ||||# | +|| +| |+|+ | + |||+ Sbjct 54 SVAEKTKEQANAVSEAVVSSVNTVAAKTVE#EAENIAVTSGVVRKEDL---KPSAPQQ 107 |
| Xenopus tropicalis | Query 56 AEKTKEQVTNVGG----AVVTGVTAVAQKTVE-----------#GAGSIAAATGFVKKDQL 100 |||||| | || || || +||+|| | #|||+|||||| ||||+ Sbjct 30 AEKTKEGVLYVGSKTRDGVVQGVASVAEKTKEQASQLGGAVMS#GAGNIAAATGLVKKDEF 89 |
| cattle, brain, Peptide, 134 aa | Query 56 AEKTKEQVTNVGG----AVVTGVTAVAQKTVE-----------#GAGSIAAATGFVKKDQL 100 |||||| | || || || +||+|| | #|||+|||||| |||++ Sbjct 30 AEKTKEGVLYVGSKTKEGVVQGVASVAEKTKEQASHLGGAVFS#GAGNIAAATGLVKKEEF 89 |
| Tetraodon nigroviridis | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVKK 97 ||| +| +| || ||| | |+| |||#| ++||++| + + Sbjct 10 TVANRTVDQANIVGDAVVGGANEVSQATVE#GVENVAASSGLISQ 53 |
| Danio rerio | Query 54 TVAEKTKEQVTNVGGAVVTGVTAVAQKTVE#GAGSIAAATGFVK 96 |||+|| +| + | | |++| ||#| ++ |+ | || Sbjct 54 TVAQKTTDQANLMSDTAVAGANEVSEKAVE#GLETMVASAGLVK 96 |
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