logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0037 : Vimentin

[ CaMP Format ]

* Basic Information

OrganismMus musculus (house mouse)
Protein Namesvimentin [Mus musculus]; vimentin; Vimentin
Gene NamesVim; vimentin
Gene Locus2 10.04 cM; chromosome 2
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_035831 NM_011701 22352 P20152 N/A N/A N/A mmu:22352

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 466 aa

Average Mass: 53.687 kDa

Monoisotopic Mass: 53.655 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 18 ---
--- cleavage 20 ---
--- cleavage 32 ---
--- cleavage 37 ---
--- cleavage 40 ---
--- cleavage 52 ---
--- cleavage 63 ---
--- cleavage 70 ---
--- cleavage 91 ---
AAA domain 1. 152402743.034.7
--- cleavage 264 (inside AAA domain 152..402) ---

3. Sequence Information

Fasta Sequence: SB0037.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

10 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 3028449] Fischer S, Vandekerckhove J, Ampe C, Traub P, Weber K, Protein-chemical identification of the major cleavage sites of the Ca2+ proteinase on murine vimentin, the mesenchymal intermediate filament protein. Biol Chem Hoppe Seyler. 1986 Nov;367(11):1147-52.

Cleavage sites (±10aa)

[Site 1] SSYRRMFGGS18-GTSSRPSSNR

Ser18 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser9Ser10Tyr11Arg12Arg13Met14Phe15Gly16Gly17Ser18
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly19Thr20Ser21Ser22Arg23Pro24Ser25Ser26Asn27Arg28

Sequence conservation (by blast)

[Site 2] YRRMFGGSGT20-SSRPSSNRSY

Thr20 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Tyr11Arg12Arg13Met14Phe15Gly16Gly17Ser18Gly19Thr20
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser21Ser22Arg23Pro24Ser25Ser26Asn27Arg28Ser29Tyr30

Sequence conservation (by blast)

[Site 3] RPSSNRSYVT32-TSTRTYSLGS

Thr32 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Arg23Pro24Ser25Ser26Asn27Arg28Ser29Tyr30Val31Thr32
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Thr33Ser34Thr35Arg36Thr37Tyr38Ser39Leu40Gly41Ser42

Sequence conservation (by blast)

[Site 4] RSYVTTSTRT37-YSLGSALRPS

Thr37 Tyr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Arg28Ser29Tyr30Val31Thr32Thr33Ser34Thr35Arg36Thr37
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Tyr38Ser39Leu40Gly41Ser42Ala43Leu44Arg45Pro46Ser47

Sequence conservation (by blast)

[Site 5] VTTSTRTYSL40-GSALRPSTSR

Leu40 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Val31Thr32Thr33Ser34Thr35Arg36Thr37Tyr38Ser39Leu40
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly41Ser42Ala43Leu44Arg45Pro46Ser47Thr48Ser49Arg50

Sequence conservation (by blast)

[Site 6] ALRPSTSRSL52-YSSSPGGAYV

Leu52 Tyr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala43Leu44Arg45Pro46Ser47Thr48Ser49Arg50Ser51Leu52
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Tyr53Ser54Ser55Ser56Pro57Gly58Gly59Ala60Tyr61Val62

Sequence conservation (by blast)

[Site 7] SSSPGGAYVT63-RSSAVRLRSS

Thr63 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser54Ser55Ser56Pro57Gly58Gly59Ala60Tyr61Val62Thr63
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Arg64Ser65Ser66Ala67Val68Arg69Leu70Arg71Ser72Ser73

Sequence conservation (by blast)

[Site 8] YVTRSSAVRL70-RSSVPGVRLL

Leu70 Arg

iTraq-117 Signal 8882.1 () for YVTRSSAVRLR

iTraq-117 Signal 351.4 () for TRSSAVRLRSSVPGVRLL

iTraq-117 Signal 13888.7 () for SSVPGVRLL

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Tyr61Val62Thr63Arg64Ser65Ser66Ala67Val68Arg69Leu70
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Arg71Ser72Ser73Val74Pro75Gly76Val77Arg78Leu79Leu80

Sequence conservation (by blast)

[Site 9] DSVDFSLADA91-INTEFKNTRT

Ala91 Ile

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Asp82Ser83Val84Asp85Phe86Ser87Leu88Ala89Asp90Ala91
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ile92Asn93Thr94Glu95Phe96Lys97Asn98Thr99Arg100Thr101

Sequence conservation (by blast)

[Site 10] QIDVDVSKPD264-LTAALRDVRQ

Asp264 Leu

iTraq-117 Signal 22351.3 () for AALRDVRQ

iTraq-117 Signal 4566.0 () for ALRDVRQ

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gln255Ile256Asp257Val258Asp259Val260Ser261Lys262Pro263Asp264
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Leu265Thr266Ala267Ala268Leu269Arg270Asp271Val272Arg273Gln274

Sequence conservation (by blast)

* References

[PubMed ID: 24142690] Matsuyama M, Tanaka H, Inoko A, Goto H, Yonemura S, Kobori K, Hayashi Y, Kondo E, Itohara S, Izawa I, Inagaki M, Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract via aneuploidy and senescence in lens epithelial cells. J Biol Chem. 2013 Dec 13;288(50):35626-35. doi: 10.1074/jbc.M113.514737. Epub

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