SB0038 : RhoA, ras homolog gene family, member A
[ CaMP Format ]
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | transforming protein RhoA precursor [Homo sapiens]; transforming protein RhoA precursor; transforming protein RhoA; oncogene RHO H12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; h12; rho cDNA clone 12; ras homolog gene family, member A; Transforming protein RhoA; Rho cDNA clone 12 |
| Gene Names | RHOA; ARH12, ARHA, RHO12; ARH12; ARHA; RHO12; ras homolog family member A |
| Gene Locus | 3p21.3; chromosome 3 |
| GO Function | Not available |
* Information From OMIM
Function: The small guanosine triphosphatase (GTP) Rho regulates remodeling of the actin cytoskeleton during cell morphogenesis and motility. In their Figure 3C, Maekawa et al. (1999) diagrammed proposed signaling pathways for Rho-induced remodeling of the actin cytoskeleton. They demonstrated that active Rho signals to its downstream effector ROCK1 (OMIM:601702), which phosphorylates and activates LIM kinase (see OMIM:601329). LIM kinase, in turn, phosphorylates cofilin (OMIM:601442), inhibiting its actin-depolymerizing activity.
* Structure Information
1. Primary Information
Length: 193 aa
Average Mass: 21.768 kDa
Monoisotopic Mass: 21.754 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| Signal recognition particle receptor beta subunit 1. | 110 | 121 | 113.0 | 0.0 |
3. Sequence Information
Fasta Sequence: SB0038.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB: 1A2B (X-ray; 240 A; A=1-181), 1CC0 (X-ray; 500 A; A/C=1-190), 1CXZ (X-ray; 220 A; A=1-181), 1DPF (X-ray; 200 A; A=1-180), 1FTN (X-ray; 210 A; A=1-193), 1KMQ (X-ray; 155 A; A=4-181), 1LB1 (X-ray; 281 A; B/D/F/H=1-190), 1OW3 (X-ray; 180 A; B=1-193), 1S1C (X-ray; 260 A; A/B=1-181), 1TX4 (X-ray; 165 A; B=3-179), 1X86 (X-ray; 322 A; B/D/F/H=1-193), 1XCG (X-ray; 250 A; B/F=3-180), 2RGN (X-ray; 350 A; C/F=1-193), 3KZ1 (X-ray; 270 A; E/F=1-181), 3LW8 (X-ray; 185 A; A/B/C/D=2-181), 3LWN (X-ray; 228 A; A/B=2-181), 3LXR (X-ray; 168 A; A=2-181), 3MSX (X-ray; 165 A; A=1-180), 3T06 (X-ray; 284 A; B/F=3-180), 4D0N (X-ray; 210 A; A=1-184), 4XH9 (X-ray; 200 A; B/E=2-180)
* Cleavage Information
1 [sites] cleaved by Calpain 1
Source Reference: [PubMed ID: 11964413] Kulkarni S, Goll DE, Fox JE, Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J Biol Chem. 2002 Jul 5;277(27):24435-41. Epub 2002 Apr 18.
Cleavage sites (±10aa)
[Site 1] FEMATRAALQ180-ARRGKKKSGC
Gln180 
Ala
iTraq-117 Signal 154.8 (
) for FEMATRAALQAR
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Phe171 | Glu172 | Met173 | Ala174 | Thr175 | Arg176 | Ala177 | Ala178 | Leu179 | Gln180 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala181 | Arg182 | Arg183 | Gly184 | Lys185 | Lys186 | Lys187 | Ser188 | Gly189 | Cys190 |
Sequence conservation (by blast)
* References
[PubMed ID: 24584070] Yoo HY, Sung MK, Lee SH, Kim S, Lee H, Park S, Kim SC, Lee B, Rho K, Lee JE, Cho KH, Kim W, Ju H, Kim J, Kim SJ, Kim WS, Lee S, Ko YH, A recurrent inactivating mutation in RHOA GTPase in angioimmunoblastic T cell lymphoma. Nat Genet. 2014 Apr;46(4):371-5. doi: 10.1038/ng.2916. Epub 2014 Mar 2.