CaMPDB :: Substrate

SB0047 : Protamine-Z

* Basic Information

Organism	Clupea harengus (Atlantic herring)
Protein Names	RecName: Full=Protamine-Z; AltName: Full=Clupeine-Z; Protamine-Z; Clupeine-Z
Gene Names	Not available
Gene Locus	Not available
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
P69010	N/A	N/A	P69010	N/A	N/A	N/A	N/A

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 31 aa

Average Mass: 4.165 kDa

Monoisotopic Mass: 4.163 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value

3. Sequence Information

Fasta Sequence: SB0047.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 7INS (X-ray; 200 A; G=-)

* Cleavage Information

2 [sites]

Source Reference: [PubMed ID: 2993267] Hayashi M, Inomata M, Nakamura M, Imahori K, Kawashima S, Hydrolysis of protamine by calcium-activated neutral protease (CANP). J Biochem. 1985 May;97(5):1363-70.

Cleavage sites (±10aa)

[Site 1] SRRASRPVRR¹⁵-RRPRRVSRRR

Arg¹⁵ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ser⁶	Arg⁷	Arg⁸	Ala⁹	Ser¹⁰	Arg¹¹	Pro¹²	Val¹³	Arg¹⁴	Arg¹⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹⁶	Arg¹⁷	Pro¹⁸	Arg¹⁹	Arg²⁰	Val²¹	Ser²²	Arg²³	Arg²⁴	Arg²⁵

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
ARRRRSRRASRPVRRRRPRRVSRRRRARRRR

[Site 2] RRRRPRRVSR²³-RRRARRRR

Arg²³ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Arg¹⁴	Arg¹⁵	Arg¹⁶	Arg¹⁷	Pro¹⁸	Arg¹⁹	Arg²⁰	Val²¹	Ser²²	Arg²³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg²⁴	Arg²⁵	Arg²⁶	Ala²⁷	Arg²⁸	Arg²⁹	Arg³⁰	Arg³¹	-	-

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
ARRRRSRRASRPVRRRRPRRVSRRRRARRRR

* References

[PubMed ID: 1772633] Balschmidt P, Hansen FB, Dodson EJ, Dodson GG, Korber F, Structure of porcine insulin cocrystallized with clupeine Z. Acta Crystallogr B. 1991 Dec 1;47 ( Pt 6):975-86.