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SB0048 : Annexin I

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesannexin A1 [Homo sapiens]; annexin A1; annexin I (lipocortin I); annexin-1; calpactin-2; calpactin II; chromobindin-9; phospholipase A2 inhibitory protein; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Gene NamesANXA1; ANX1, LPC1; ANX1; LPC1; annexin A1
Gene Locus9q21.13; chromosome 9
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_000691 NM_000700 301 P04083 151690 HGNC:533 01060 hsa:301

* Information From OMIM

Function: Walther et al. (2000) showed that ANXA1 acts through the formyl peptide receptor (FPR; OMIM:136537) on human neutrophils. Peptides derived from the unique N-terminal domain of ANXA1 serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

* Structure Information

1. Primary Information

Length: 346 aa

Average Mass: 38.714 kDa

Monoisotopic Mass: 38.690 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 26 ---
Myb DNA-binding like 1. 5010318.00.0
Myb DNA-binding like 2. 12515121.00.0
Myb DNA-binding like 3. 21326325.00.0
Myb DNA-binding like 4. 28430521.00.0

3. Sequence Information

Fasta Sequence: SB0048.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1AIN (X-ray; 250 A; A=33-346), 1BO9 (NMR; -; A=41-113), 1QLS (X-ray; 230 A; D=2-12)

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 2540167] Ando Y, Imamura S, Hong YM, Owada MK, Kakunaga T, Kannagi R, Enhancement of calcium sensitivity of lipocortin I in phospholipid binding induced by limited proteolysis and phosphorylation at the amino terminus as analyzed by phospholipid affinity column chromatography. J Biol Chem. 1989 Apr 25;264(12):6948-55.

Cleavage sites (±10aa)

[Site 1] EEQEYVQTVK26-SSKGGPGSAV

Lys26 Ser

iTraq-117 Signal 721.2 () for EEQEYVQTVK

iTraq-117 Signal 2656.4 () for SSKGGPGSAV

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Glu17Glu18Gln19Glu20Tyr21Val22Gln23Thr24Val25Lys26
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser27Ser28Lys29Gly30Gly31Pro32Gly33Ser34Ala35Val36

Sequence conservation (by blast)

* References

[PubMed ID: 24480285] Yu CJ, Ko CJ, Hsieh CH, Chien CT, Huang LH, Lee CW, Jiang CC, Proteomic analysis of osteoarthritic chondrocyte reveals the hyaluronic acid-regulated proteins involved in chondroprotective effect under oxidative stress. J Proteomics. 2014 Mar 17;99:40-53. doi: 10.1016/j.jprot.2014.01.016. Epub 2014

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