CaMPDB :: Substrate

SB0050 : EGFR, ErbB-1, Epidermal growth factor receptor

* Basic Information

Organism	Homo sapiens (human)
Protein Names	epidermal growth factor receptor isoform a precursor [Homo sapiens]; epidermal growth factor receptor isoform a precursor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell proliferation-inducing protein 61; cell growth inhibiting protein 40; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Epidermal growth factor receptor; 2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Gene Names	EGFR; ERBB, ERBB1, HER1; ERBB; ERBB1; HER1; epidermal growth factor receptor
Gene Locus	7p12; chromosome 7
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_005219	NM_005228	1956	P00533	131550	HGNC:3236	N/A	hsa:1956

* Information From OMIM

Description: EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development (Wang et al., 2004).

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* Structure Information

1. Primary Information

Length: 1210 aa

Average Mass: 134.276 kDa

Monoisotopic Mass: 134.190 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
Receptor L domain 1.	57	167	1.0	0.7
Growth factor receptor domain IV 1.	294	342	18.0	10.6
Furin-like cysteine rich region 1.	554	598	49.0	11.7
Furin-like cysteine rich region 2.	618	636	49.0	3.6
GRB2-binding adapter (GAPT) 1.	650	687	13.0	0.3
--- cleavage 683 (inside GRB2-binding adapter (GAPT) 650..687) ---
--- cleavage 733 ---
Phosphotransferase enzyme family 1.	824	860	162.0	0.0

3. Sequence Information

Fasta Sequence: SB0050.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1DNQ (Model; -; A=25-336), 1DNR (Model; -; A=337-645), 1IVO (X-ray; 330 A; A/B=25-646), 1M14 (X-ray; 260 A; A=695-1022), 1M17 (X-ray; 260 A; A=695-1022), 1MOX (X-ray; 250 A; A/B=25-525), 1NQL (X-ray; 280 A; A=25-642), 1XKK (X-ray; 240 A; A=695-1022), 1YY9 (X-ray; 260 A; A=25-642), 1Z9I (NMR; -; A=669-721), 2EB2 (X-ray; 250 A; A=695-1022), 2EB3 (X-ray; 284 A; A=695-1022), 2EXP (Model; -; A=311-326), 2EXQ (Model; -; A=27-536), 2GS2 (X-ray; 280 A; A=696-1022), 2GS6 (X-ray; 260 A; A=696-1022), 2GS7 (X-ray; 260 A; A/B=696-1022), 2ITN (X-ray; 247 A; A=696-1019), 2ITO (X-ray; 325 A; A=696-1022), 2ITP (X-ray; 274 A; A=696-1022), 2ITQ (X-ray; 268 A; A=696-1022), 2ITT (X-ray; 273 A; A=696-1022), 2ITU (X-ray; 280 A; A=696-1022), 2ITV (X-ray; 247 A; A=696-1022), 2ITW (X-ray; 288 A; A=696-1022), 2ITX (X-ray; 298 A; A=696-1022), 2ITY (X-ray; 342 A; A=696-1022), 2ITZ (X-ray; 272 A; A=696-1022), 2J5E (X-ray; 310 A; A=696-1022), 2J5F (X-ray; 300 A; A=696-1022), 2J6M (X-ray; 310 A; A=696-1022), 2JIT (X-ray; 310 A; A/B=696-1022), 2JIU (X-ray; 305 A; A/B=695-1022), 2JIV (X-ray; 350 A; A/B=695-1022), 2KS1 (NMR; -; B=634-677), 2M0B (NMR; -; A/B=634-677), 2M20 (NMR; -; A/B=642-697), 2RF9 (X-ray; 350 A; A/B=696-1022), 2RFD (X-ray; 360 A; A/B=702-1022), 2RFE (X-ray; 290 A; A/B/C/D=702-1022), 2RGP (X-ray; 200 A; A=702-1016), 3B2U (X-ray; 258 A; A/B/E/I/M/P/S/V=335-538), 3B2V (X-ray; 330 A; A=25-642), 3BEL (X-ray; 230 A; A=702-1016), 3BUO (X-ray; 260 A; A/C=1063-1075), 3C09 (X-ray; 320 A; A/D=336-538), 3G5V (X-ray; 200 A; C=311-326), 3G5Y (X-ray; 159 A; E=311-326), 3GOP (X-ray; 280 A; A=669-1022), 3GT8 (X-ray; 296 A; A/B/C/D=696-1022), 3IKA (X-ray; 290 A; A/B=694-1022), 3LZB (X-ray; 270 A; A/B/C/D/E/F/G/H=696-983), 3NJP (X-ray; 330 A; A/B=25-638), 3OB2 (X-ray; 210 A; A=1063-1074), 3OP0 (X-ray; 252 A; C/D=1066-1076), 3P0Y (X-ray; 180 A; A=334-538), 3PFV (X-ray; 227 A; C/D=1066-1076), 3POZ (X-ray; 150 A; A=696-1022), 3QWQ (X-ray; 275 A; A=1-642), 3UG1 (X-ray; 275 A; A=695-1022), 3UG2 (X-ray; 250 A; A=695-1022), 3VJN (X-ray; 234 A; A=695-1022), 3VJO (X-ray; 264 A; A=695-1022), 3VRP (X-ray; 152 A; B=1062-1074), 3VRR (X-ray; 200 A; C=1062-1074), 3W2O (X-ray; 235 A; A=698-1022), 3W2P (X-ray; 205 A; A=698-1022), 3W2Q (X-ray; 220 A; A=698-1022), 3W2R (X-ray; 205 A; A=698-1022), 3W2S (X-ray; 190 A; A=696-1022), 3W32 (X-ray; 180 A; A=696-1022), 3W33 (X-ray; 170 A; A=696-1022), 4G5J (X-ray; 280 A; A=696-1022), 4G5P (X-ray; 317 A; A/B=696-1022), 4HJO (X-ray; 275 A; A=696-1022), 4I1Z (X-ray; 300 A; A=695-1022), 4I20 (X-ray; 334 A; A=695-1022), 4I21 (X-ray; 337 A; A/B=695-1022), 4I22 (X-ray; 171 A; A=695-1022), 4I23 (X-ray; 280 A; A=695-1022), 4I24 (X-ray; 180 A; A/B=695-1022), 4JQ7 (X-ray; 273 A; A=696-1021), 4JQ8 (X-ray; 283 A; A=696-1021), 4JR3 (X-ray; 270 A; A=696-1021), 4JRV (X-ray; 280 A; A=696-1021), 4KRL (X-ray; 285 A; A=335-538), 4KRM (X-ray; 266 A; A/C/E/G/I/K=335-538), 4KRO (X-ray; 305 A; A=25-642), 4KRP (X-ray; 282 A; A=25-642), 4LI5 (X-ray; 264 A; A=696-1020), 4LL0 (X-ray; 400 A; A/B=694-1022), 4LQM (X-ray; 250 A; A=694-1022), 4LRM (X-ray; 353 A; A/B/C/D/E=694-1022), 4R5S (X-ray; 300 A; A=696-1022), 4RIW (X-ray; 310 A; B/D=682-1022), 4RIX (X-ray; 310 A; B/D=682-1022), 4RIY (X-ray; 298 A; B/D=682-1022), 4RJ4 (X-ray; 278 A; A=695-1022), 4RJ5 (X-ray; 310 A; A=695-1022), 4RJ6 (X-ray; 270 A; A=695-1022), 4RJ7 (X-ray; 255 A; A=695-1022), 4RJ8 (X-ray; 250 A; A=695-1022), 4TKS (X-ray; 320 A; A=695-1022), 4WKQ (X-ray; 185 A; A=696-1022), 4WRG (X-ray; 190 A; A=696-1022)

* Cleavage Information

7 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 8055914] Gregoriou M, Willis AC, Pearson MA, Crawford C, The calpain cleavage sites in the epidermal growth factor receptor kinase domain. Eur J Biochem. 1994 Jul 15;223(2):455-64.

Cleavage sites (±10aa)

[Site 1] VRKRTLRRLL⁶⁸³-QERELVEPLT

Leu⁶⁸³ Gln

iTraq-117 Signal 3131.0 () for VRKRTLRRLL

iTraq-117 Signal 3354.7 () for QERELVEPLT

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val⁶⁷⁴	Arg⁶⁷⁵	Lys⁶⁷⁶	Arg⁶⁷⁷	Thr⁶⁷⁸	Leu⁶⁷⁹	Arg⁶⁸⁰	Arg⁶⁸¹	Leu⁶⁸²	Leu⁶⁸³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Gln⁶⁸⁴	Glu⁶⁸⁵	Arg⁶⁸⁶	Glu⁶⁸⁷	Leu⁶⁸⁸	Val⁶⁸⁹	Glu⁶⁹⁰	Pro⁶⁹¹	Leu⁶⁹²	Thr⁶⁹³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
LLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFK

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	124.00	9	cell growth inhibiting protein 40
2	Homo sapiens	124.00	3	epidermal growth factor receptor
3	synthetic construct	124.00	1	epidermal growth factor receptor
4	N/A	118.00	16	EGF receptor
5	Mus musculus	118.00	9	AF275367_1 epidermal growth factor receptor
6	Rattus norvegicus	114.00	9	epidermal growth factor receptor
7	Avian erythroblastosis virus	112.00	3	gag,v-erb-A,v-erb-B
8	Rous-associated virus type 1	112.00	1	S69372_2 v-erbB gene product
9	Rous-associated virus type 1	111.00	1	polyprotein
10	Canis familiaris	100.00	8	PREDICTED: similar to Epidermal growth factor rec
11	Sus scrofa	100.00	1	epidermal growth factor receptor
12	Tetraodon nigroviridis	93.20	7	unnamed protein product
13	Mesocricetus auratus	80.10	1	ERBB2_MESAU Receptor tyrosine-protein kinase erbB
14	Gallus gallus	75.90	3	AF121963_1 receptor tyrosine kinase precursor
15	Canis lupus familiaris	73.90	1	erbB-2
16	Felis catus	73.20	2	v-erb-b2 erythroblastic leukemia viral oncogene h
17	synthetic construct	72.40	2	v-erb-b2 erythroblastic leukemia viral oncogene-l
18	Danio rerio	72.00	4	epidermal growth factor receptor
19	Danio rerio	72.00	1	epidermal growth factor receptor
20	Xiphophorus xiphidium	69.30	1	receptor tyrosine kinase proto-oncogene
21	Xiphophorus sp.	68.60	1	XMRK_XIPMA Melanoma receptor tyrosine-protein kin
22	Ovis aries	67.00	1	erythroblastic leukemia viral oncogene-like 2
23	Pongo pygmaeus	51.60	1	ERBB3_PONPY Receptor tyrosine-protein kinase erbB
24	Ciona intestinalis	47.00	2	epidermal growth factor receptor
25	Anopheles gambiae	40.40	1	putative epidermal growth factor receptor
26	Takifugu rubripes	37.40	1	erbB3
27	Cricetinae	33.50	1	AAB29944.1
28	Blastopirellula marina DSM 3645	32.00	1	hypothetical protein DSM3645_01811
29	Thermobifida fusca YX	31.20	1	multiple sugar transport system permease protein
30	Bacillus anthracis	30.80	1	hypothetical protein pxo2_59

Top-ranked sequences

organism	matching
Homo sapiens	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713
Homo sapiens	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713
synthetic construct	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713
N/A	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| ++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 656 LLFIVVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQAHLRILKETEFK 715
Mus musculus	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| ++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 656 LLFIVVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQAHLRILKETEFK 715
Rattus norvegicus	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| ++\|\|\|\|\|\|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 655 LLFIVVVALGIGLFMRRRQLVRKRTLRRLL#QERELVEPLTPSGEAPNQAHLRILKETEFK 714
Avian erythroblastosis virus	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| \|+\|\| \|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 496 LLCLVVVGLGIGLYLRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQAHLRILKETEFK 555
Rous-associated virus type 1	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| \|+\|\| \|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 79 LLCLVVVGLGIGLYLRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQAHLRILKETEFK 138
Rous-associated virus type 1	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| \|+\|\| \|\|\|\|\|++\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 228 LLCLVVVGLGIGLYLRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQAHLRILKETEFK 287
Canis familiaris	Query 667 FMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1055 FLRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 1101
Sus scrofa	Query 667 FMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 667 FLRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713
Tetraodon nigroviridis	Query 666 LFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 + +\|\|\|\|\| \|\|\|\|+\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Sbjct 636 VLLRRRHIKRKRTMRRLL#QERELVEPLTPSGEAPNQALLRILKEPEFK 683
Mesocricetus auratus	Query 654 LLLLLVVALGIGLFM-RRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEF 712 +\|\| \|\|+ + +\|+ + \|\|\| +\|\| \|+\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| +\|\|\|\|\|\|\| Sbjct 661 ILLFLVIGVVVGILIKRRRQKIRKYTMRRLL#QETELVEPLTPSGAMPNQAQMRILKETEL 720 Query 713 K 713 + Sbjct 721 R 721
Gallus gallus	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \| +++++ \| +++\|\|+ \| +\|\| \|\|\| \|# \| \|\|\|\|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\| \| Sbjct 509 LFIIVIMGLTFAVYVRRKSIKKKRALRRFL#-ETELVEPLTPSGTAPNQAQLRILKETELK 567
Canis lupus familiaris	Query 666 LFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \| \|\|\| +\|\| \|+\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| +\|\|\|\|\|\|\| + Sbjct 673 LIKRRRQKIRKYTMRRLL#QETELVEPLTPSGAMPNQAQMRILKETELR 720
Felis catus	Query 669 RRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|\| +\|\| \|+\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| +\|\|\|\|\|\|\| + Sbjct 677 RRRQKIRKYTMRRLL#QETELVEPLTPSGAMPNQAQMRILKETELR 721
synthetic construct	Query 666 LFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \| \|\|+ +\|\| \|+\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| +\|\|\|\|\|\|\| + Sbjct 674 LIKRRQQKIRKYTMRRLL#QETELVEPLTPSGAMPNQAQMRILKETELR 721
Danio rerio	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| +++\|\|\|+ + +\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 653 LLAFVILALGVAVLLRRRHIRRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 712
Danio rerio	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\| +++\|\|\|+ + +\|\|\|\|\| \|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 653 LLAFVILALGVAVLLRRRHIRRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 712
Xiphophorus xiphidium	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|+ ++\|\|\| \| + +\|\|\| \| \|\|\|\|+\|\|\|\|#\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 650 LLITVIVALLIVVLLRRRRIKRKRTIRRLL#QEKELVEPLTPSGQAPNQAFLRILKETEFK 709
Xiphophorus sp.	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|+ ++\|\|\| \| + +\|\|\| \| \|\|\|\|+\| \|\|#\|\|+\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| \|\|\|\|\|\|\|\|\|\| Sbjct 652 LLITVIVALLIVVLLRRRRIKRKRTIRCLL#QEKELVEPLTPSGQAPNQAFLRILKETEFK 711
Ovis aries	Query 669 RRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|\|\| +\|\| \|+\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| +\|\|\|\|\|\|\| + Sbjct 28 RRRQKIRKYTMRRLL#QETELVEPLTPSGAMPNQAQMRILKETELR 72
Pongo pygmaeus	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 \|+++ ++ \| \|+ \| \| \| \|\| +\|\| \|#+ \| +\|\|\| \|\| \| \|+ \| \|\| \|\|\|\| \| Sbjct 652 LVVIFMMLGGTFLYWRGRRIQNKRAMRRYL#ERGESIEPLDPS-EKANKVLARIFKETELK 710
Ciona intestinalis	Query 658 LVVALGIGLFMRRRHIVRKRT--LRRL-----------L#QER-ELVEPLTPSGEAPNQAL 703 \|+ ++ \| \|\|+ \| +\|\|\| +\|+\| +# \| \|+\|\|+\|\|\|\| \|\|\|\|\| Sbjct 55 LLFSIFFGCRYRRKQIQKKRTQSMRKLGIDPHSPEEQNM#DPRVRLMEPMTPSGVAPNQAQ 114 Query 704 LRILKETEFK 713 \|\|\|+\|\| \| + Sbjct 115 LRIVKEAELR 124
Anopheles gambiae	Query 655 LLLLVVALGIGLFMRRRHIVRKRTLRRL--L#QERELVEPLTPSGEAPNQALLRILKETEF 712 \|+\|\| \| \|\| + \|\| +\| ++ \|# \| \|\|\| \|\| \|\| \|\|\|+\|\| \| Sbjct 775 LILLCVVFGIAFVLFSRHKNKKDAVKMTMAL#AGCEDSEPLRPSNVGPNLTKLRIIKEAEI 834 Query 713 K 713 + Sbjct 835 R 835
Takifugu rubripes	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSGEAPNQALLRILKETEFK 713 + \|++ \|\|+ \|+ \| \| \|\|\| +\|\| \|#+ \| \|\|\| \| \|\| + \|\|\|\| ++ + Sbjct 651 IFCLVLFFLGM-LYHRGLAIRRKRAMRRYL#ESGESFEPLGP-GEKGTKVHARILKPSDLR 708
Cricetinae	Query 654 LLLLLVVALGIGLFM-RRRHIVRKRTLRRLL#QERE 687 +\|\| \|\|+ + +\|+ + \|\|\| +\|\| \|+\|\|\|\|#\|\| \| Sbjct 12 ILLFLVIGVVVGILIKRRRQKIRKYTMRRLL#QETE 46
Blastopirellula marina DSM 3645	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELVEPLTPSG 696 \| \| \|\| \|+\|\| \| + +\|\|\| \|#++\|\|\| \|\|+ +\| Sbjct 214 LYLFTYVAAGLGLLMTTSFL----SLRRYL#RQRELPMPLSMAG 252
Thermobifida fusca YX	Query 655 LLLLVVALGIGLFMRRRHIVRKRTLRRLL# 683 \|\|+ \|\| \|\|+ \| \| \| +\| \|\|\| \|+\|# Sbjct 317 LLVFVVGLGLALLMHTRTPLRGRTLYRIL# 345
Bacillus anthracis	Query 654 LLLLLVVALGIGLFMRRRHIVRKRTLRRLL#QERELV 689 \|\|\| \|\|+\|\| \| +\|+ \| + \| ++ \|#\|\|+\|\|+ Sbjct 14 LLLSLVIALMINIFVFRTYTVEGESMEPTL#QEQELI 49

[Site 2] GTVYKGLWIP⁷³³-EGEKVKIPVA

Pro⁷³³ Glu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gly⁷²⁴	Thr⁷²⁵	Val⁷²⁶	Tyr⁷²⁷	Lys⁷²⁸	Gly⁷²⁹	Leu⁷³⁰	Trp⁷³¹	Ile⁷³²	Pro⁷³³

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Glu⁷³⁴	Gly⁷³⁵	Glu⁷³⁶	Lys⁷³⁷	Val⁷³⁸	Lys⁷³⁹	Ile⁷⁴⁰	Pro⁷⁴¹	Val⁷⁴²	Ala⁷⁴³

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
LRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEA

Summary

#	organism	max score	hits	top seq
1	N/A	125.00	14	EGF receptor
2	Rattus norvegicus	125.00	7	epidermal growth factor receptor
3	Homo sapiens	125.00	7	epidermal growth factor receptor isoform a
4	Mus musculus	125.00	6	epidermal growth factor receptor isoform 1
5	synthetic construct	125.00	2	epidermal growth factor receptor
6	Canis familiaris	125.00	2	PREDICTED: similar to Epidermal growth factor rec
7	Homo sapiens	125.00	1	epidermal growth factor receptor
8	Sus scrofa	125.00	1	epidermal growth factor receptor
9	Rous-associated virus type 1	123.00	1	S69372_2 v-erbB gene product
10	Rous-associated virus type 1	120.00	1	polyprotein
11	synthetic construct	119.00	2	unnamed protein product
12	Avian erythroblastosis virus	119.00	2	gag,v-erb-A,v-erb-B
13	Danio rerio	117.00	2	epidermal growth factor receptor
14	Danio rerio	117.00	1	epidermal growth factor receptor
15	Tetraodon nigroviridis	116.00	3	unnamed protein product
16	Gallus gallus	112.00	2	erbB-2
17	Felis catus	111.00	2	v-erb-b2 erythroblastic leukemia viral oncogene h
18	Canis lupus familiaris	111.00	1	erbB-2
19	Mesocricetus auratus	111.00	1	ERBB2_MESAU Receptor tyrosine-protein kinase erbB
20	Xiphophorus sp.	107.00	1	XMRK_XIPMA Melanoma receptor tyrosine-protein kin
21	Xiphophorus xiphidium	107.00	1	receptor tyrosine kinase proto-oncogene
22	Ovis aries	94.40	1	erythroblastic leukemia viral oncogene-like 2
23	Drosophila melanogaster	83.20	6	mutant epidermal growth factor receptor

Top-ranked sequences

organism	matching
N/A	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Rattus norvegicus	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Homo sapiens	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Mus musculus	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
synthetic construct	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Canis familiaris	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Homo sapiens	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Sus scrofa	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 704 LRILKETEFKKVKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Rous-associated virus type 1	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 129 LRILKETEFKKVKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 188
Rous-associated virus type 1	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 278 LRILKETEFKKVKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 337
synthetic construct	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763
Avian erythroblastosis virus	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 546 LRILKETEFKKVKVLGFGAFGTVYKGLWIP#EGEKVTIPVAIKELREATSPKANKEILDEA 605
Danio rerio	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|+\|#\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 703 LRILKETEFKKIKVLGSGAFGTVHKGLWVP#EGENVKIPVAIKVLREATSPKANKEIMDEA 762
Danio rerio	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|+\|#\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 703 LRILKETEFKKIKVLGSGAFGTVHKGLWVP#EGENVKIPVAIKVLREATSPKANKEIMDEA 762
Tetraodon nigroviridis	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|#\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\| Sbjct 674 LRILKEPEFKKIKVLGSGAFGTVYKGLWVP#EGEDVKIPVAIKVLREATSPKANKDILDEA 733
Gallus gallus	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 +\|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#+\|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 701 MRILKETELKKVKVLGSGAFGTVYKGIWIP#DGESVKIPVAIKVLRENTSPKANKEILDEA 760
Felis catus	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 +\|\|\|\|\|\|\| +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#+\|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 712 MRILKETELRKVKVLGSGAFGTVYKGIWIP#DGENVKIPVAIKVLRENTSPKANKEILDEA 771
Canis lupus familiaris	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 +\|\|\|\|\|\|\| +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#+\|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 711 MRILKETELRKVKVLGSGAFGTVYKGIWIP#DGENVKIPVAIKVLRENTSPKANKEILDEA 770
Mesocricetus auratus	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 +\|\|\|\|\|\|\| +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#+\|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 712 MRILKETELRKVKVLGSGAFGTVYKGIWIP#DGENVKIPVAIKVLRENTSPKANKEILDEA 771
Xiphophorus sp.	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#+\|\| ++\|\|\|\|\|\| \|\|\|\|\|\|\|\| \|+\|+\|\|\|\| Sbjct 702 LRILKETEFKKDRVLGSGAFGTVYKGLWNP#DGENIRIPVAIKVLREATSPKVNQEVLDEA 761
Xiphophorus xiphidium	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|\|\|\|\|\|\|\|\| +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|#+\|\| ++\|\|\|\|\|\| \|\|\|\|\|\|\|\| \|+\|+\|\|\|\| Sbjct 700 LRILKETEFKKDRVLGSGAFGTVYKGLWNP#DGENIRIPVAIKVLREATSPKVNQEVLDEA 759
Ovis aries	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKAN 756 +\|\|\|\|\|\|\| +\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|#+\|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\| Sbjct 63 MRILKETELRKVKVLGSGAFGTVYKGIWIP#DGENVKIPVAIKVLRENTSPKAN 115
Drosophila melanogaster	Query 704 LRILKETEFKKIKVLGSGAFGTVYKGLWIP#EGEKVKIPVAIKELREATSPKANKEILDEA 763 \|\|\|+\|+\|\| +\| \|\|\| \|\|\|\| \|\|\|\|+\|+\|#\|\|\| \|\|\|\|\|\|\|\|\|\| ++\| ++++\| \| \|\| Sbjct 830 LRIVKDTELRKGGVLGMGAFGRVYKGVWVP#EGENVKIPVAIKELLKSTGAESSEEFLREA 889

[Site 3] QGFFSSPSTS¹⁰³⁰-RTPLLSSLSA

Ser¹⁰³⁰ Arg

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Gln¹⁰²¹	Gly¹⁰²²	Phe¹⁰²³	Phe¹⁰²⁴	Ser¹⁰²⁵	Ser¹⁰²⁶	Pro¹⁰²⁷	Ser¹⁰²⁸	Thr¹⁰²⁹	Ser¹⁰³⁰

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Arg¹⁰³¹	Thr¹⁰³²	Pro¹⁰³³	Leu¹⁰³⁴	Leu¹⁰³⁵	Ser¹⁰³⁶	Ser¹⁰³⁷	Leu¹⁰³⁸	Ser¹⁰³⁹	Ala¹⁰⁴⁰

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
LMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPI

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	123.00	8	epidermal growth factor receptor isoform a
2	synthetic construct	123.00	1	epidermal growth factor receptor
3	Homo sapiens	123.00	1	epidermal growth factor receptor
4	N/A	107.00	10	EGF receptor
5	Mus musculus	107.00	8	epidermal growth factor receptor isoform 1
6	Mus musculus	106.00	1	epidermal growth factor receptor
7	Avian erythroblastosis virus	100.00	2	gag,v-erb-A,v-erb-B
8	Rous-associated virus type 1	99.80	1	S69372_2 v-erbB gene product
9	Rous-associated virus type 1	99.40	1	polyprotein
10	Canis familiaris	85.50	2	PREDICTED: similar to Epidermal growth factor rec
11	Sus scrofa	82.00	1	epidermal growth factor receptor
12	Rattus norvegicus	81.60	7	epidermal growth factor receptor
13	Danio rerio	56.60	1	epidermal growth factor receptor
14	Danio rerio	56.60	1	epidermal growth factor receptor
15	Gallus gallus	49.70	2	erbB-2
16	Felis catus	45.40	1	v-erb-b2 erythroblastic leukemia viral oncogene h
17	Canis lupus familiaris	43.90	1	erbB-2
18	Mesocricetus auratus	43.50	1	ERBB2_MESAU Receptor tyrosine-protein kinase erbB
19	synthetic construct	43.50	1	v-erb-b2 erythroblastic leukemia viral oncogene-l
20	Tetraodon nigroviridis	41.20	2	unnamed protein product
21	Trypanosoma cruzi strain CL Brener	33.10	2	protein phosphatase 1 catalitic subunit, putative
22	Oryza sativa (japonica cultivar-group)	32.00	1	Os09g0397300
23	Clostridium tetani E88	32.00	1	hypothetical protein pE88_13

Top-ranked sequences

organism	matching
Homo sapiens	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060
synthetic construct	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060
Homo sapiens	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060
N/A	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| + Sbjct 1003 LMDEEDMEDVVDADEYLIPQQGFFNSPSTS#RTPLLSSLSATSNNSTVACINRNG--SCRV 1060
Mus musculus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| + Sbjct 1003 LMDEEDMEDVVDADEYLIPQQGFFNSPSTS#RTPLLSSLSATSNNSTVACINRNG--SCRV 1060
Mus musculus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| + Sbjct 35 LMDEEDMEDVVDADEYLIPQQGFFNSPSTS#RTPLLSSLSATSNNSTVACINRNG--SCRV 92
Avian erythroblastosis virus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNG 1054 \|\|+\|\|\|\|+\|+\|\|\|\|\|\|\|+\| \|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 843 LMEEEDMEDIVDADEYLVPHQGFFNSPSTS#RTPLLSSLSATSNNSATKCIDRNG 896
Rous-associated virus type 1	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|+\|\|\|\|+\|+\|\|\|\|\|\|\|+\| \|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \| \|+ Sbjct 426 LMEEEDMEDIVDADEYLVPHQGFFNSPSTS#RTPLLSSLSATSNNSATNCIDRNG-QGHPV 484
Rous-associated virus type 1	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|+\|\|\|\|+\|+\|\|\|\|\|\|\|+\| \|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \| \|+ Sbjct 575 LMEEEDMEDIVDADEYLVPHQGFFNSPSTS#RTPLLSSLSATSNNSATNCIDRNG-QGHPV 633
Canis familiaris	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\| +\|\|+ Sbjct 1389 LMDEEDMEDVVDADEYLIPQQGFFHSPSTS#RTPLLSSLSATSNSSNVACIDRNG--TCPL 1446
Sus scrofa	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| \|\|+\|\|#\|\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|+\|\|\|\| \|\| \|+ Sbjct 1001 LMDEEDMEDVVDADEYLVPQQGFFHSPATS#RTPLLSSLSATSSTPAVACVDRNG-QSYPL 1059
Rattus norvegicus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQSCPI 1060 \|\|+\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|+\|\|\| \|\| + Sbjct 1002 LMEEEDMEDVVDADEYLIPQQGFFNSPSTS#RTPLLSSLSANSNSSTVACINRNG--SCRV 1059
Danio rerio	Query 1006 DMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNG 1054 ++\|+ \|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|#\|\| \|\| \|+\| \|+\| \| \|\|\| Sbjct 1004 ELDEAVDADEYLVPNHSFFSSPSTS#RTQLLHSVSL--NSSFGNCNSRNG 1050
Danio rerio	Query 1006 DMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNG 1054 ++\|+ \|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\|#\|\| \|\| \|+\| \|+\| \| \|\|\| Sbjct 1004 ELDEAVDADEYLVPNHSFFSSPSTS#RTQLLHSVSL--NSSFGNCNSRNG 1050
Gallus gallus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNGLQS 1057 \|++\|\|\|\|\|\|+\|\|\|+\|\|\|+\| \|\|\|\|\|+ ++ # \| \| +\|+\| + + \| +\| Sbjct 997 LLEEEDMDDLVDAEEYLVPHQGFFSAETS-#-TAYRSRISSTRSTAETPADTTEGEES 1051
Felis catus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFF 1024 \|+++\|\|\| \|+\|\|\|+\|\|\|+\|\|\|\|\|\| Sbjct 1008 LLEDEDMGDLVDAEEYLVPQQGFF 1031
Canis lupus familiaris	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFS---SPSTS#RTPLLSSLSATSNNS 1045 \|++++\|\| \|+\|\|\|+\|\|\|+\|\|\|\|\|\| +\| # \| \|+++ \| Sbjct 1007 LLEDDDMGDLVDAEEYLVPQQGFFCPEPTPGAG#GTAHRRHRSSSTRNG 1054
Mesocricetus auratus	Query 1001 LMDEEDMDDVVDADEYLIPQQGFF 1024 \|++++\|\| \|+\|\|\|+\|\|\|+\|\|\|\|\|\| Sbjct 1008 LLEDDDMGDLVDAEEYLVPQQGFF 1031
synthetic construct	Query 1001 LMDEEDMDDVVDADEYLIPQQGFF 1024 \|++++\|\| \|+\|\|\|+\|\|\|+\|\|\|\|\|\| Sbjct 1008 LLEDDDMGDLVDAEEYLVPQQGFF 1031
Tetraodon nigroviridis	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSS 1037 \|+\|\|\|\|+\|+++\|\|\|\|\|\|+\|\| + \|\| +# \| + \| Sbjct 181 LLDEEDLDNLMDADEYLVPQGYTLAPPSYT#VRPRVDS 217
Trypanosoma cruzi strain CL Brener	Query 1001 LMDEEDMDDVVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATS------NNSTVACIDRNG 1054 + \| \|+\| \| \| + + ++\|\| \|\| +#+ \|\|+ \|\|\|+ \| \| \|+\|+ \| Sbjct 306 FLKENDLDMVCRAHQ--VVEEGFQFFPSNT#KRHLLTIFSATNYCNEFGNRGGVLCVDKEG 363 Query 1055 LQSCPI 1060 + \| \| Sbjct 364 VCSLLI 369
Oryza sativa (japonica cultivar-group)	Query 1010 VVDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNN 1044 ++\| \| \|+\|\| \| \|\|\|+ # +\|+\|\|\| \|\| Sbjct 599 LLDYDGTLMPQTSFGKSPSSK#TIDMLNSLSRDQNN 633
Clostridium tetani E88	Query 1011 VDADEYLIPQQGFFSSPSTS#RTPLLSSLSATSNNSTVACIDRNG----LQSCPI 1060 +\| \|\|\|\| +\|\| \| \|\|#+\| \|+ \|+\| + + \| ++\| + +\|\|+ Sbjct 61 LDGKEYLIGEQGVEDSSETS#KTNLIHKLAAYTAITQVLDSNKNNKVQLVLACPL 114

[Site 4] IDRNGLQSCP¹⁰⁵⁹-IKEDSFLQRY

Pro¹⁰⁵⁹ Ile

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Ile¹⁰⁵⁰	Asp¹⁰⁵¹	Arg¹⁰⁵²	Asn¹⁰⁵³	Gly¹⁰⁵⁴	Leu¹⁰⁵⁵	Gln¹⁰⁵⁶	Ser¹⁰⁵⁷	Cys¹⁰⁵⁸	Pro¹⁰⁵⁹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ile¹⁰⁶⁰	Lys¹⁰⁶¹	Glu¹⁰⁶²	Asp¹⁰⁶³	Ser¹⁰⁶⁴	Phe¹⁰⁶⁵	Leu¹⁰⁶⁶	Gln¹⁰⁶⁷	Arg¹⁰⁶⁸	Tyr¹⁰⁶⁹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPV

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	121.00	5	epidermal growth factor receptor isoform a
2	Homo sapiens	121.00	1	epidermal growth factor receptor
3	synthetic construct	121.00	1	epidermal growth factor receptor
4	N/A	103.00	9	EGF receptor
5	Mus musculus	102.00	3	epidermal growth factor receptor isoform 1
6	Mus musculus	102.00	1	epidermal growth factor receptor
7	Rous-associated virus type 1	92.00	1	S69372_2 v-erbB gene product
8	Rous-associated virus type 1	91.70	1	polyprotein
9	Sus scrofa	82.80	1	epidermal growth factor receptor
10	Canis familiaris	82.40	1	PREDICTED: similar to Epidermal growth factor rec
11	Rattus norvegicus	78.20	1	epidermal growth factor receptor
12	Avian erythroblastosis virus	56.20	2	gag,v-erb-A,v-erb-B
13	Danio rerio	33.50	1	epidermal growth factor receptor
14	Danio rerio	33.50	1	epidermal growth factor receptor
15	synthetic construct	31.20	1	v-erb-b2 erythroblastic leukemia viral oncogene-l

Top-ranked sequences

organism	matching
Homo sapiens	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089
Homo sapiens	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089
synthetic construct	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089
N/A	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| #+\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| \|\|\|\| Sbjct 1032 SRTPLLSSLSATSNNSTVACINRNG--SCR#VKEDAFLQRYSSDPTGAVTEDNIDDAFLPV 1089
Mus musculus	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| #+\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| \|\|\|\| Sbjct 1032 SRTPLLSSLSATSNNSTVACINRNG--SCR#VKEDAFLQRYSSDPTGAVTEDNIDDAFLPV 1089
Mus musculus	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| \|\| #+\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| \|\|\|\| Sbjct 64 SRTPLLSSLSATSNNSTVACINRNG--SCR#VKEDAFLQRYSSDPTGAVTEDNIDDAFLPV 121
Rous-associated virus type 1	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLP 1088 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \| \|#++\|\|\|\|+\|\|\|\|\|\|\|\|\| \|+\|\|\|\| \|\|\| Sbjct 455 SRTPLLSSLSATSNNSATNCIDRNG-QGHP#VREDSFVQRYSSDPTGNFLEESIDDGFLP 512
Rous-associated virus type 1	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLP 1088 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \| \|#++\|\|\|\|+\|\|\|\|\|\|\|\|\| \|+\|\|\|\| \|\|\| Sbjct 604 SRTPLLSSLSATSNNSATNCIDRNG-QGHP#VREDSFVQRYSSDPTGNFLEESIDDGFLP 661
Sus scrofa	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLP 1088 \|\|\|\|\|\|\|\|\|\|\|\|\|+ \|\|\|+\|\|\|\| \|\| \|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|\|\| Sbjct 1030 SRTPLLSSLSATSSTPAVACVDRNG-QSYP#LKEDSFLQRYSSDPTGALTEDSLDDTFLP 1087
Canis familiaris	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLP 1088 \|\|\|\|\|\|\|\|\|\|\|\|\|\|+\| \|\|\|\|\|\|\|\| +\|\|#+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|\|\|\|\| Sbjct 1418 SRTPLLSSLSATSNSSNVACIDRNG--TCP#LKEDSFLQRYSSDPTGTLTEDNIDDTFLP 1474
Rattus norvegicus	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDSIDDTFLPV 1089 \|\|\|\|\|\|\|\|\|\|\| \|\|+\|\|\|\|\|\|+\|\|\| \|\| #+\|\|\|+\|\|\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|\|\|\|\|\| Sbjct 1031 SRTPLLSSLSANSNSSTVACINRNG--SCR#VKEDAFLQRYSSDPTSVLTEDNIDDTFLPV 1088
Avian erythroblastosis virus	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFL-------QRYSSDPTGALTEDSI 1082 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\| \|#++\|\| \|\| \| \|+ \|+ ++ \| Sbjct 872 SRTPLLSSLSATSNNSATKCIDRNGGH--P#VREDGFLPAPEYVNQLMPKKPSTAMVQNQI 929
Danio rerio	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTE 1079 \|\|\| \|\| \|+\| \|+\| \| \|\|\| \|#++\|+\| + \|\| \|\|\| \| Sbjct 1028 SRTQLLHSVSL--NSSFGNCNSRNG-NGYP#VRENSMVLRYIPDPTERFQE 1074
Danio rerio	Query 1030 SRTPLLSSLSATSNNSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTE 1079 \|\|\| \|\| \|+\| \|+\| \| \|\|\| \|#++\|+\| + \|\| \|\|\| \| Sbjct 1028 SRTQLLHSVSL--NSSFGNCNSRNG-NGYP#VRENSMVLRYIPDPTERFQE 1074
synthetic construct	Query 1031 RTPLLSSLSATSN--NSTVACIDRNGLQSCP#IKEDSFLQRYSSDPTGALTEDS 1081 \|+\|\| \| \| \|+ + + \|\|\|\| \|# + \| \|\|\|\|\| \|\|\| \| ++ Sbjct 1072 RSPLAPSEGAGSDVFDGDLGMGAAKGLQSLP#THDPSPLQRYSEDPTVPLPSET 1124

[Site 5] LTEDSIDDTF¹⁰⁸⁶-LPVPEYINQS

Phe¹⁰⁸⁶ Leu

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Leu¹⁰⁷⁷	Thr¹⁰⁷⁸	Glu¹⁰⁷⁹	Asp¹⁰⁸⁰	Ser¹⁰⁸¹	Ile¹⁰⁸²	Asp¹⁰⁸³	Asp¹⁰⁸⁴	Thr¹⁰⁸⁵	Phe¹⁰⁸⁶

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Leu¹⁰⁸⁷	Pro¹⁰⁸⁸	Val¹⁰⁸⁹	Pro¹⁰⁹⁰	Glu¹⁰⁹¹	Tyr¹⁰⁹²	Ile¹⁰⁹³	Asn¹⁰⁹⁴	Gln¹⁰⁹⁵	Ser¹⁰⁹⁶

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
SCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLN

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	127.00	3	cell growth inhibiting protein 40
2	Homo sapiens	127.00	1	epidermal growth factor receptor
3	synthetic construct	127.00	1	epidermal growth factor receptor
4	Canis familiaris	121.00	1	PREDICTED: similar to Epidermal growth factor rec
5	Sus scrofa	117.00	1	epidermal growth factor receptor
6	N/A	116.00	9	EGF receptor
7	Mus musculus	116.00	3	AF275367_1 epidermal growth factor receptor
8	Rattus norvegicus	115.00	2	epidermal growth factor receptor
9	Mus musculus	115.00	1	epidermal growth factor receptor
10	Rous-associated virus type 1	79.00	1	polyprotein
11	Rous-associated virus type 1	78.60	1	S69372_2 v-erbB gene product
12	Avian erythroblastosis virus	39.30	2	gag,v-erb-A,v-erb-B
13	Gallus gallus	33.90	1	AF121963_1 receptor tyrosine kinase precursor
14	Chloroflexus aurantiacus J-10-fl	31.20	1	hypothetical protein Caur_2525
15	Lettuce big-vein associated virus	31.20	1	protein 5

Top-ranked sequences

organism	matching
Homo sapiens	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116
Homo sapiens	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116
synthetic construct	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116
Canis familiaris	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 +\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|\|\|#\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1443 TCPLKEDSFLQRYSSDPTGTLTEDNIDDTF#LPAPEYINQSVPKRPAGSVQNPVYHNQPLN 1502
Sus scrofa	Query 1059 PIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|\|#\|\| \|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 1058 PLKEDSFLQRYSSDPTGALTEDSLDDTF#LPAPEYVNQSVPKRPAGSVQNPVYHNQPLS 1115
N/A	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\| +\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| \|#\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 1057 SCRVKEDAFLQRYSSDPTGAVTEDNIDDAF#LPVPEYVNQSVPKRPAGSVQNPVYHNQPLH 1116
Mus musculus	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\| +\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| \|#\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 1057 SCRVKEDAFLQRYSSDPTGAVTEDNIDDAF#LPVPEYVNQSVPKRPAGSVQNPVYHNQPLH 1116
Rattus norvegicus	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\| +\|\|\|+\|\|\|\|\|\|\|\|\|\| \|\|\|\|+\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 1056 SCRVKEDAFLQRYSSDPTSVLTEDNIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLH 1115
Mus musculus	Query 1057 SCPIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGSVQNPVYHNQPLN 1116 \|\| +\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\|+\|\|\| \|#\|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+ Sbjct 89 SCRVKEDAFLQRYSSDPTGAVTEDNIDDAF#LPVPEYVNQSVPKRPAGSVQNPVYHNQPLD 148
Rous-associated virus type 1	Query 1059 PIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGS-VQNPVYHNQPL 1115 \|++\|\|\|\|+\|\|\|\|\|\|\|\|\| \|+\|\|\|\| \|#\|\| \|\|\|+\|\| +\|\|+\|+ + \|\|\| +\|+\| \| Sbjct 632 PVREDSFVQRYSSDPTGNFLEESIDDGF#LPAPEYVNQLMPKKPSTAMVQNQIYNNISL 689
Rous-associated virus type 1	Query 1059 PIKEDSFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQSVPKRPAGS-VQNPVYHNQPLN 1116 \|++\|\|\|\|+\|\|\|\|\|\|\|\|\| \|+\|\|\|\| \|#\|\| \|\|\|+\|\| +\|\|+\|+ + \|\|\| +\|+\| \| Sbjct 483 PVREDSFVQRYSSDPTGNFLEESIDDGF#LPAPEYVNQLMPKKPSTAMVQNQIYNNISLT 541
Avian erythroblastosis virus	Query 1083 DDTF#LPVPEYINQSVPKRPAGS-VQNPVYH 1111 +\| \|#\|\| \|\|\|+\|\| +\|\|+\|+ + \|\|\| +\|+ Sbjct 902 EDGF#LPAPEYVNQLMPKKPSTAMVQNQIYN 931
Gallus gallus	Query 1061 KEDSFLQRYSSDPT---------GALTEDSI---------DDTF#LPVPEYINQSVPKRPA 1102 \|\|\|\| \|\|\|\|+\|\|\| \| \| \|\| \| # \|\| \| \| \| +\| Sbjct 952 KEDSSTQRYSADPTVFIPERVIRGELDEDGYMTPMRDKPKTDYL#NPVEE--NPFVSRRKN 1009 Query 1103 GSVQ---NPVYHNQP 1114 \| +\| \|\| \|\|\| \| Sbjct 1010 GDLQAVDNPEYHNAP 1024
Chloroflexus aurantiacus J-10-fl	Query 1064 SFLQRYSSDPTGALTEDSIDDTF#LPVPEYINQS 1096 \|\| + \| \|\| \| + \| \| +\| #+\| \|+\|++ \| Sbjct 146 SFFEHYMRDPQGYIVGDWIGETP#VPRPDYLSSS 178
Lettuce big-vein associated virus	Query 1058 CPIKE--DSFLQRYSSDPTGALTEDSIDDTF#LPVPEY 1092 \|\|+\| \|\| \|\|\| + + +\|+ \| \|\|#\|\|+ \|\| Sbjct 259 CPLKRVYDSLLQRIPTGVHISHSEEKGDPTF#LPLAEY 295

[Site 6] VQPTCVNSTF¹¹⁵¹-DSPAHWAQKG

Phe¹¹⁵¹ Asp

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Val¹¹⁴²	Gln¹¹⁴³	Pro¹¹⁴⁴	Thr¹¹⁴⁵	Cys¹¹⁴⁶	Val¹¹⁴⁷	Asn¹¹⁴⁸	Ser¹¹⁴⁹	Thr¹¹⁵⁰	Phe¹¹⁵¹

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Asp¹¹⁵²	Ser¹¹⁵³	Pro¹¹⁵⁴	Ala¹¹⁵⁵	His¹¹⁵⁶	Trp¹¹⁵⁷	Ala¹¹⁵⁸	Gln¹¹⁵⁹	Lys¹¹⁶⁰	Gly¹¹⁶¹

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
DPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEA

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	132.00	2	cell growth inhibiting protein 40
2	Homo sapiens	132.00	1	epidermal growth factor receptor
3	synthetic construct	132.00	1	epidermal growth factor receptor
4	N/A	110.00	8	EGF receptor
5	Mus musculus	110.00	4	AF275367_1 epidermal growth factor receptor
6	Rattus norvegicus	108.00	4	epidermal growth factor receptor
7	Canis familiaris	104.00	2	PREDICTED: similar to Epidermal growth factor rec
8	Sus scrofa	102.00	1	epidermal growth factor receptor
9	Tetraodon nigroviridis	39.70	1	unnamed protein product
10	Rous-associated virus type 1	35.40	1	polyprotein
11	Rous-associated virus type 1	33.50	1	S69372_2 v-erbB gene product
12	Gallus gallus	32.70	1	AF121963_1 receptor tyrosine kinase precursor
13	Avian erythroblastosis virus	32.00	2	gag,v-erb-A,v-erb-B

Top-ranked sequences

organism	matching
Homo sapiens	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181
Homo sapiens	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181
synthetic construct	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181
N/A	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKE 1180 \| \|\|\|+\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\|++\| \|#+\|\|\| \| \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1122 DLHYQNPHSNAVGNPEYLNTAQPTCLSSGF#NSPALWIQKGSHQMSLDNPDYQQDFFPKE 1180
Mus musculus	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKE 1180 \| \|\|\|+\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\|++\| \|#+\|\|\| \| \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1122 DLHYQNPHSNAVGNPEYLNTAQPTCLSSGF#NSPALWIQKGSHQMSLDNPDYQQDFFPKE 1180
Rattus norvegicus	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181 \| \|\|\|+\|\|\| \|\| \|\|\|\|\|\|\| \|\|\|\|++\| \|#\|\| \| \| \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1121 DLHYQNPHSNAVSNPEYLNTAQPTCLSSGF#DSSALWIQKGSHQMSLDNPDYQQDFFPKEA 1180
Canis familiaris	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181 \|\|\|\|\|+\|\|\| \|\| \|\|\|\|\|\|\| \|\|\|\|\|\| #\| \|+ \| \|+ +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1508 DPHYQNPHSNAVDNPEYLNT-HPTCVNSVL#DRPSLWTQEANHQISLDNPDYQQDFFPKEA 1566
Sus scrofa	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPKEA 1181 \|\|\|\|\|+ \|\| \|\|\|\|\|\|\|\|\|\| +\| \|+\| #\| \|\| \|\|\| \|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|\|\|\|\| Sbjct 1121 DPHYQNSHSNAVGNPEYLNTPRPACINGGL#DGPAFWAQTGSHQINLDNPDYQQAFFPKEA 1180
Tetraodon nigroviridis	Query 1136 PEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFP 1178 \|\|\|\|\| \| \|+ \| \|# +\| \|\| + \| \|+\|\|\|\|\|\|\|\|\| \| Sbjct 1035 PEYLNEV-PSSSNFPF#-TPN--TQKYAPQNSIDNPDYQQDFTP 1073
Rous-associated virus type 1	Query 1122 DPHYQDPHSTAVGNPEYLNT---VQPTCVNS 1149 \| \|\|+ \|\|\|\|\| \|\|\|\|\|\| \| \|\| \| Sbjct 698 DSRYQNSHSTAVDNPEYLNMAVRTLPECVVS 728
Rous-associated virus type 1	Query 1122 DPHYQDPHSTAVGNPEYLN 1140 \| \|\|+ \|\|\|\|\| \|\|\|\|\|\| Sbjct 549 DSRYQNSHSTAVDNPEYLN 567
Gallus gallus	Query 1122 DPHYQDPHSTAVGNPEYLNTVQPTCVNSTF#DSPAHWAQKGSHQISLDNPDYQQDFFPK 1179 +\| \| + + + \| \|\|\| \| \|#\|+\| +\| + +\| +\|\|\| \|++ \| Sbjct 1037 EPLYLNTFANTLENAEYLKNNLPEKAKKAF#DNPDYWNHSLPPRSTLQHPDYLQEYSTK 1094
Avian erythroblastosis virus	Query 1122 DPHYQDPHSTAVGNPEYL 1139 \| \|\|+ \|\|\|\|\| \|\|\|\|\| Sbjct 944 DSRYQNSHSTAVDNPEYL 961

[Site 7] FFPKEAKPNG¹¹⁸⁵-IFKGSTAENA

Gly¹¹⁸⁵ Ile

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

Phe¹¹⁷⁶	Phe¹¹⁷⁷	Pro¹¹⁷⁸	Lys¹¹⁷⁹	Glu¹¹⁸⁰	Ala¹¹⁸¹	Lys¹¹⁸²	Pro¹¹⁸³	Asn¹¹⁸⁴	Gly¹¹⁸⁵

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ile¹¹⁸⁶	Phe¹¹⁸⁷	Lys¹¹⁸⁸	Gly¹¹⁸⁹	Ser¹¹⁹⁰	Thr¹¹⁹¹	Ala¹¹⁹²	Glu¹¹⁹³	Asn¹¹⁹⁴	Ala¹¹⁹⁵

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
HWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA

Summary

#	organism	max score	hits	top seq
1	Homo sapiens	124.00	2	cell growth inhibiting protein 40
2	synthetic construct	123.00	1	epidermal growth factor receptor
3	Homo sapiens	123.00	1	epidermal growth factor receptor
4	N/A	110.00	5	EGF receptor
5	Mus musculus	110.00	3	AF275367_1 epidermal growth factor receptor
6	Rattus norvegicus	110.00	1	epidermal growth factor receptor
7	Canis familiaris	102.00	1	PREDICTED: similar to Epidermal growth factor rec
8	Sus scrofa	93.20	1	epidermal growth factor receptor
9	Tetraodon nigroviridis	38.90	1	unnamed protein product
10	Danio rerio	38.10	1	epidermal growth factor receptor
11	Danio rerio	38.10	1	epidermal growth factor receptor
12	Listeria innocua Clip11262	30.00	1	hypothetical protein lin1079

Top-ranked sequences

organism	matching
Homo sapiens	Query 1156 HWAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1156 HWAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210
synthetic construct	Query 1156 HWAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1156 HWAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210
Homo sapiens	Query 1156 HWAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1156 HWAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210
N/A	Query 1157 WAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \| \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 1157 WIQKGSHQMSLDNPDYQQDFFPKETKPNG#IFKGPTAENAEYLRVAPPSSEFIGA 1210
Mus musculus	Query 1157 WAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \| \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 1157 WIQKGSHQMSLDNPDYQQDFFPKETKPNG#IFKGPTAENAEYLRVAPPSSEFIGA 1210
Rattus norvegicus	Query 1157 WAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \| \|\|\|\|\|\|+\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|#\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\| \|\| Sbjct 1156 WIQKGSHQMSLDNPDYQQDFFPKEAKPNG#IFKGPTAENAEYLRVAPPSSEFSGA 1209
Canis familiaris	Query 1157 WAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \| \|+ +\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|#\|\|\|\| \|\|\|\|+\|\|\|\|\|\| \|\|\|\|\|\|\| Sbjct 1542 WTQEANHQISLDNPDYQQDFFPKEAKSNG#IFKGPAAENADYLRVAPPSSEFIGA 1595
Sus scrofa	Query 1157 WAQKGSHQISLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYLRVAPQSSEFIGA 1210 \|\|\| \|\|\|\|\|+\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|#\| \|\| \|\|\|\|\|\|\|\| \|\| \|\|+ \|\| Sbjct 1156 WAQTGSHQINLDNPDYQQAFFPKEAKSNG#ICKGPAAENAEYLRAAPASSDLTGA 1209
Tetraodon nigroviridis	Query 1159 QKGSHQISLDNPDYQQDFFPK-EAKPNG#IFKGSTAENAEYLRV 1200 \|\| + \| \|+\|\|\|\|\|\|\|\|\| \| + \|\|# \|\|\| \|\| \| Sbjct 1054 QKYAPQNSIDNPDYQQDFTPTFKTHTNG#HIPA--AENTEYTSV 1094
Danio rerio	Query 1166 SLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYL 1198 \|+\|\|\|\|\|\|\|\|\| \| \| \| + # \|+\| \|\|+ Sbjct 1154 SMDNPDYQQDFCPLELKTHT#NGHLPAAQNQEYM 1186
Danio rerio	Query 1166 SLDNPDYQQDFFPKEAKPNG#IFKGSTAENAEYL 1198 \|+\|\|\|\|\|\|\|\|\| \| \| \| + # \|+\| \|\|+ Sbjct 1154 SMDNPDYQQDFCPLELKTHT#NGHLPAAQNQEYM 1186
Listeria innocua Clip11262	Query 1157 WAQKGSHQISLDNPDYQQDFFPKEAKP 1183 \| \|\| \|+ \|\|\|\|\| \|+ \| \|\|\| Sbjct 59 WVDKGHHKASLDNPAVQETI--KRAKP 83

* References

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