logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0054 : Glucagon

[ CaMP Format ]

* Basic Information

OrganismRattus norvegicus (Norway rat)
Protein Namesglucagon [Rattus norvegicus]; glucagon; glucagon-like peptide-1; Glucagon; Glicentin; Glicentin-related polypeptide; GRPP; Oxyntomodulin; OXM; OXY; Glucagon-like peptide 1; GLP-1; Glucagon-like peptide 1(7-37); GLP-1(7-37); Glucagon-like peptide 1(7-36); GLP-1(7-36); Glucagon-like peptide 2; GLP-2
Gene NamesGcg; glucagon
Gene Locus3q22-q24; chromosome 3
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_036839 NM_012707 24952 P06883 N/A N/A N/A rno:24952

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 180 aa

Average Mass: 20.846 kDa

Monoisotopic Mass: 20.833 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 67 ---
--- cleavage 69 ---
Putative mycoplasma lipoprotein, C-terminal region 1. 7011019.00.2
--- cleavage 74 (inside Putative mycoplasma lipoprotein, C-terminal region 70..110) ---
--- cleavage 77 (inside Putative mycoplasma lipoprotein, C-terminal region 70..110) ---
--- cleavage 79 (inside Putative mycoplasma lipoprotein, C-terminal region 70..110) ---
Putative mycoplasma lipoprotein, C-terminal region 2. 11212616.00.0
Putative mycoplasma lipoprotein, C-terminal region 3. 16017716.00.1

3. Sequence Information

Fasta Sequence: SB0054.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

5 [sites] cleaved by Calpain 1 and 2

Source Reference: [PubMed ID: 6092335] Sasaki T, Kikuchi T, Yumoto N, Yoshimura N, Murachi T, Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates. J Biol Chem. 1984 Oct 25;259(20):12489-94.

Cleavage sites (±10aa)

[Site 1] FTSDYSKYLD67-SRRAQDFVQW

Asp67 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Phe58Thr59Ser60Asp61Tyr62Ser63Lys64Tyr65Leu66Asp67
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser68Arg69Arg70Ala71Gln72Asp73Phe74Val75Gln76Trp77

Sequence conservation (by blast)

[Site 2] SDYSKYLDSR69-RAQDFVQWLM

Arg69 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser60Asp61Tyr62Ser63Lys64Tyr65Leu66Asp67Ser68Arg69
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Arg70Ala71Gln72Asp73Phe74Val75Gln76Trp77Leu78Met79

Sequence conservation (by blast)

[Site 3] YLDSRRAQDF74-VQWLMNTKRN

Phe74 Val

iTraq-117 Signal 6465.1 () for NTKRN

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Tyr65Leu66Asp67Ser68Arg69Arg70Ala71Gln72Asp73Phe74
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Val75Gln76Trp77Leu78Met79Asn80Thr81Lys82Arg83Asn84

Sequence conservation (by blast)

[Site 4] SRRAQDFVQW77-LMNTKRNRNN

Trp77 Leu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser68Arg69Arg70Ala71Gln72Asp73Phe74Val75Gln76Trp77
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Leu78Met79Asn80Thr81Lys82Arg83Asn84Arg85Asn86Asn87

Sequence conservation (by blast)

[Site 5] RAQDFVQWLM79-NTKRNRNNIA

Met79 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Arg70Ala71Gln72Asp73Phe74Val75Gln76Trp77Leu78Met79
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asn80Thr81Lys82Arg83Asn84Arg85Asn86Asn87Ile88Ala89

Sequence conservation (by blast)

* References

[PubMed ID: 19560500] Dozier KC, Cureton EL, Kwan RO, Curran B, Sadjadi J, Victorino GP, Glucagon-like peptide-1 protects mesenteric endothelium from injury during inflammation. Peptides. 2009 Sep;30(9):1735-41. doi: 10.1016/j.peptides.2009.06.019. Epub 2009

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