* Cleavage Information
2 [sites] cleaved by Calpain 11
Source Reference: [PubMed ID: 1681901] Kiuchi K, Kiuchi K, Titani K, Fujita K, Suzuki K, Nagatsu T, Limited proteolysis of tyrosine hydroxylase by Ca(2+)-activated neutral protease (calpain). Biochemistry. 1991 Oct 29;30(43):10416-9.
Cleavage sites (±10aa)
[Site 1] GFRRAVSELD22-AKQAEAIMSP
Asp22 
Ala
iTraq-117 Signal 748.0 (
) for GFRRAVSELDAKQAEAIM
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Gly13 | Phe14 | Arg15 | Arg16 | Ala17 | Val18 | Ser19 | Glu20 | Leu21 | Asp22 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala23 | Lys24 | Gln25 | Ala26 | Glu27 | Ala28 | Ile29 | Met30 | Ser31 | Pro32 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MPTPNAASPQAKGFRRAVSELDAKQAEAIMSPRFVGRRQSLIQDARKEREKA
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
105.00 |
12 |
TY3H_BOVIN Tyrosine 3-monooxygenase (Tyrosine 3-h |
| 2 |
Bos taurus |
105.00 |
2 |
tyrosine hydroxylase |
| 3 |
Canis lupus familiaris |
97.80 |
1 |
tyrosine hydroxylase |
| 4 |
Homo sapiens |
96.30 |
9 |
Tyrosine hydroxylase |
| 5 |
Mus musculus |
87.80 |
3 |
tyrosine hydroxylase |
| 6 |
Rattus norvegicus |
87.80 |
2 |
tyrosine hydroxylase |
| 7 |
synthetic construct |
87.80 |
1 |
chimeric tyrosine hydroxylase |
| 8 |
Homo sapiens |
80.90 |
2 |
tyrosine hydroxylase isoform 2 |
| 9 |
Gallus gallus |
76.30 |
1 |
tyrosine hydroxylase |
| 10 |
Macaca mulatta |
60.10 |
1 |
tyrosine hydroxylase type 1 |
| 11 |
Anguilla anguilla |
48.50 |
1 |
TY3H_ANGAN Tyrosine 3-monooxygenase (Tyrosine 3-h |
| 12 |
Lates calcarifer |
40.80 |
1 |
tyrosine hydroxylase 1 |
| 13 |
Danio rerio |
37.00 |
1 |
tyrosine hydroxylase |
| 14 |
Takifugu rubripes |
35.40 |
1 |
tyrosine 3-monooxygenase |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKEREKA 52
||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKEREKA 52
|
| Bos taurus |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKEREKA 52
||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKEREKA 52
|
| Canis lupus familiaris |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKER 49
||||| ||||||||||||||||#||||||||||||+|||||||+||||||
Sbjct 1 MPTPNTASPQAKGFRRAVSELD#AKQAEAIMSPRFIGRRQSLIEDARKER 49
|
| Homo sapiens |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKERE 50
||||+| +||||||||||||||#||||||||||||+|||||||+|||||||
Sbjct 1 MPTPDATTPQAKGFRRAVSELD#AKQAEAIMSPRFIGRRQSLIEDARKERE 50
|
| Mus musculus |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKERE 50
||||+|+||| ||||||||| |# |||||+ ||||+|||||||+|||||||
Sbjct 1 MPTPSASSPQPKGFRRAVSEQD#TKQAEAVTSPRFIGRRQSLIEDARKERE 50
|
| Rattus norvegicus |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKERE 50
||||+| ||| ||||||||| |#||||||+ ||||+|||||||+|||||||
Sbjct 1 MPTPSAPSPQPKGFRRAVSEQD#AKQAEAVTSPRFIGRRQSLIEDARKERE 50
|
| synthetic construct |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKERE 50
||||+|+||| ||||||||| |# |||||+ ||||+|||||||+|||||||
Sbjct 1 MPTPSASSPQPKGFRRAVSEQD#TKQAEAVTSPRFIGRRQSLIEDARKERE 50
|
| Homo sapiens |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIM---------------------------SPR 33
||||+| +||||||||||||||#|||||||| |||
Sbjct 1 MPTPDATTPQAKGFRRAVSELD#AKQAEAIMGAPGPSLTGSPWPGTAAPAASYTPTPRSPR 60
Query 34 FVGRRQSLIQDARKERE 50
|+|||||||+|||||||
Sbjct 61 FIGRRQSLIEDARKERE 77
|
| Gallus gallus |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQD 44
||||| ++ ||||||||||||#+|||||||||||+|||||||+|
Sbjct 1 MPTPNISTSAAKGFRRAVSELD#SKQAEAIMSPRFIGRRQSLIED 44
|
| Macaca mulatta |
Query 19 SELD#AKQAEAIMSPRFVGRRQSLIQDARKERE 50
||||#||||||+ ||||+|||||||+|||||||
Sbjct 1 SELD#AKQAEAVTSPRFIGRRQSLIEDARKERE 32
|
| Anguilla anguilla |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQDARKER 49
|| |++ | || ||||# +|++| ||||||||||||+||||||
Sbjct 1 MPISNSSGSSTKSITRAGSELD#--RADSITSPRFVGRRQSLIEDARKER 47
|
| Lates calcarifer |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQD 44
|| ++++ | ||| |||+# ++++| ||||||||||||+|
Sbjct 1 MPLSSSSTSSTKSIRRAASELE#--RSDSITSPRFVGRRQSLIED 42
|
| Danio rerio |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQD 44
|| ++++ | ||| |||+# ++++| | +|||||||||+|
Sbjct 1 MPNSSSSTSSTKSIRRAASELE#--RSDSITSQKFVGRRQSLIED 42
|
| Takifugu rubripes |
Query 1 MPTPNAASPQAKGFRRAVSELD#AKQAEAIMSPRFVGRRQSLIQD 44
|| + ++ | ||| |||+# +++++ | ||+|||||||+|
Sbjct 1 MPQSSGSTSSTKSIRRAPSELE#--RSDSVTSQRFLGRRQSLIED 42
|
[Site 2] LDAKQAEAIM30-SPRFVGRRQS
Met30 Ser
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Leu21 | Asp22 | Ala23 | Lys24 | Gln25 | Ala26 | Glu27 | Ala28 | Ile29 | Met30 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ser31 | Pro32 | Arg33 | Phe34 | Val35 | Gly36 | Arg37 | Arg38 | Gln39 | Ser40 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MPTPNAASPQAKGFRRAVSELDAKQAEAIMSPRFVGRRQSLIQDARKEREKAEAAASSSE
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
N/A |
118.00 |
12 |
TY3H_BOVIN Tyrosine 3-monooxygenase (Tyrosine 3-h |
| 2 |
Bos taurus |
118.00 |
2 |
tyrosine hydroxylase |
| 3 |
Canis lupus familiaris |
99.00 |
1 |
tyrosine hydroxylase |
| 4 |
Homo sapiens |
97.40 |
9 |
unnamed protein product |
| 5 |
Rattus norvegicus |
89.00 |
2 |
tyrosine hydroxylase |
| 6 |
Mus musculus |
88.20 |
3 |
tyrosine hydroxylase |
| 7 |
synthetic construct |
88.20 |
1 |
chimeric tyrosine hydroxylase |
| 8 |
Homo sapiens |
82.00 |
2 |
tyrosine hydroxylase isoform 2 |
| 9 |
Gallus gallus |
81.30 |
1 |
tyrosine hydroxylase |
| 10 |
Macaca mulatta |
60.50 |
1 |
tyrosine hydroxylase type 1 |
| 11 |
Anguilla anguilla |
51.20 |
1 |
TY3H_ANGAN Tyrosine 3-monooxygenase (Tyrosine 3-h |
| 12 |
Lates calcarifer |
40.00 |
1 |
tyrosine hydroxylase 1 |
| 13 |
Danio rerio |
36.60 |
1 |
tyrosine hydroxylase |
| 14 |
Takifugu rubripes |
35.00 |
1 |
tyrosine 3-monooxygenase |
Top-ranked sequences
| organism | matching |
|---|
| N/A |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKEREKAEAAASSSE 60
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKEREKAEAAASSSE 60
|
| Bos taurus |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKEREKAEAAASSSE 60
||||||||||||||||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKEREKAEAAASSSE 60
|
| Canis lupus familiaris |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKER 49
||||| ||||||||||||||||||||||||#||||+|||||||+||||||
Sbjct 1 MPTPNTASPQAKGFRRAVSELDAKQAEAIM#SPRFIGRRQSLIEDARKER 49
|
| Homo sapiens |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKERE 50
||||+| +||||||||||||||||||||||#||||+|||||||+|||||||
Sbjct 1 MPTPDATTPQAKGFRRAVSELDAKQAEAIM#SPRFIGRRQSLIEDARKERE 50
|
| Rattus norvegicus |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKERE 50
||||+| ||| ||||||||| |||||||+ #||||+|||||||+|||||||
Sbjct 1 MPTPSAPSPQPKGFRRAVSEQDAKQAEAVT#SPRFIGRRQSLIEDARKERE 50
|
| Mus musculus |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKERE 50
||||+|+||| ||||||||| | |||||+ #||||+|||||||+|||||||
Sbjct 1 MPTPSASSPQPKGFRRAVSEQDTKQAEAVT#SPRFIGRRQSLIEDARKERE 50
|
| synthetic construct |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKERE 50
||||+|+||| ||||||||| | |||||+ #||||+|||||||+|||||||
Sbjct 1 MPTPSASSPQPKGFRRAVSEQDTKQAEAVT#SPRFIGRRQSLIEDARKERE 50
|
| Homo sapiens |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM---------------------------#SPR 33
||||+| +|||||||||||||||||||||| #|||
Sbjct 1 MPTPDATTPQAKGFRRAVSELDAKQAEAIMGAPGPSLTGSPWPGTAAPAASYTPTPR#SPR 60
Query 34 FVGRRQSLIQDARKERE 50
|+|||||||+|||||||
Sbjct 61 FIGRRQSLIEDARKERE 77
|
| Gallus gallus |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQD 44
||||| ++ ||||||||||||+|||||||#||||+|||||||+|
Sbjct 1 MPTPNISTSAAKGFRRAVSELDSKQAEAIM#SPRFIGRRQSLIED 44
|
| Macaca mulatta |
Query 19 SELDAKQAEAIM#SPRFVGRRQSLIQDARKERE 50
||||||||||+ #||||+|||||||+|||||||
Sbjct 1 SELDAKQAEAVT#SPRFIGRRQSLIEDARKERE 32
|
| Anguilla anguilla |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQDARKER 49
|| |++ | || |||| +|++| #||||||||||||+||||||
Sbjct 1 MPISNSSGSSTKSITRAGSELD--RADSIT#SPRFVGRRQSLIEDARKER 47
|
| Lates calcarifer |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQD 44
|| ++++ | ||| |||+ ++++| #||||||||||||+|
Sbjct 1 MPLSSSSTSSTKSIRRAASELE--RSDSIT#SPRFVGRRQSLIED 42
|
| Danio rerio |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQD 44
|| ++++ | ||| |||+ ++++| #| +|||||||||+|
Sbjct 1 MPNSSSSTSSTKSIRRAASELE--RSDSIT#SQKFVGRRQSLIED 42
|
| Takifugu rubripes |
Query 1 MPTPNAASPQAKGFRRAVSELDAKQAEAIM#SPRFVGRRQSLIQD 44
|| + ++ | ||| |||+ +++++ #| ||+|||||||+|
Sbjct 1 MPQSSGSTSSTKSIRRAPSELE--RSDSVT#SQRFLGRRQSLIED 42
|
* References
[PubMed ID: 19207826] Douglas SA, Bunn SJ, Interferon-alpha signalling in bovine adrenal chromaffin cells: involvement of signal-transducer and activator of transcription 1 and 2, extracellular signal-regulated protein kinases 1/2 and serine 31 phosphorylation of tyrosine hydroxylase. J Neuroendocrinol. 2009 Mar;21(3):200-7. doi: 10.1111/j.1365-2826.2009.01821.x.
» More (6) « Hide Below
[PubMed ID: 17908239] ... Gelain DP, Moreira JC, Bevilaqua LR, Dickson PW, Dunkley PR, Retinol activates tyrosine hydroxylase acutely by increasing the phosphorylation of serine40 and then serine31 in bovine adrenal chromaffin cells. J Neurochem. 2007 Dec;103(6):2369-79. Epub 2007 Oct 1.
[PubMed ID: 17261361] ... Bobrovskaya L, Gelain DP, Gilligan C, Dickson PW, Dunkley PR, PACAP stimulates the sustained phosphorylation of tyrosine hydroxylase at serine 40. Cell Signal. 2007 Jun;19(6):1141-9. Epub 2007 Jan 3.
[PubMed ID: 2899135] ... Saadat S, Stehle AD, Lamouroux A, Mallet J, Thoenen H, Predicted amino acid sequence of bovine tyrosine hydroxylase and its similarity to tyrosine hydroxylases from other species. J Neurochem. 1988 Aug;51(2):572-8.
[PubMed ID: 2898537] ... D'Mello SR, Weisberg EP, Stachowiak MK, Turzai LM, Gioio AE, Kaplan BB, Isolation and nucleotide sequence of a cDNA clone encoding bovine adrenal tyrosine hydroxylase: comparative analysis of tyrosine hydroxylase gene products. J Neurosci Res. 1988 Apr;19(4):440-9.
[PubMed ID: 2895648] ... Abate C, Smith JA, Joh TH, Characterization of the catalytic domain of bovine adrenal tyrosine hydroxylase. Biochem Biophys Res Commun. 1988 Mar 30;151(3):1446-53.
[PubMed ID: 2894860] ... Haavik J, Andersson KK, Petersson L, Flatmark T, Soluble tyrosine hydroxylase (tyrosine 3-monooxygenase) from bovine adrenal medulla: large-scale purification and physicochemical properties. Biochim Biophys Acta. 1988 Mar 23;953(2):142-56.
