SB0065 : ADP-ribosyltransferase (NAD+) poly(ADP-ribose) polymerase
[ CaMP Format ]
* Basic Information
| Organism | Bos taurus (cattle) |
| Protein Names | poly [ADP-ribose] polymerase 1 [Bos taurus]; poly [ADP-ribose] polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) synthetase; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; poly[ADP-ribose] synthetase 1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; poly[ADP-ribose] synthase 1; Poly [ADP-ribose] polymerase 1; 2.4.2.30; ARTD1; Poly[ADP-ribose] synthase 1 |
| Gene Names | PARP1; ADPRT; poly (ADP-ribose) polymerase 1 |
| Gene Locus | 16; chromosome 16 |
| GO Function | Not available |
| Entrez Protein | Entrez Nucleotide | Entrez Gene | UniProt | OMIM | HGNC | HPRD | KEGG |
|---|---|---|---|---|---|---|---|
| NP_777176 | NM_174751 | 286764 | P18493 | N/A | N/A | N/A | bta:286764 |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 1016 aa
Average Mass: 113.485 kDa
Monoisotopic Mass: 113.415 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| PADR1 (NUC008) domain 1. | 20 | 26 | 15.0 | 0.7 |
| PADR1 (NUC008) domain 2. | 127 | 148 | 15.0 | 0.1 |
| Poly(ADP-ribose) polymerase catalytic domain 1. | 214 | 251 | 13.0 | 0.2 |
| Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region 1. | 322 | 359 | 41.0 | 0.3 |
| --- cleavage 384 --- | ||||
| Poly(ADP-ribose) polymerase, regulatory domain 1. | 395 | 407 | 31.0 | 0.4 |
| --- cleavage 480 --- | ||||
| --- cleavage 502 --- | ||||
| WGR domain 1. | 556 | 632 | 2.0 | 0.2 |
| --- cleavage 658 --- | ||||
| Bacterial protein of unknown function (DUF948) 1. | 753 | 770 | 52.0 | 0.0 |
| Poly(ADP-ribose) polymerase catalytic domain 2. | 799 | 1009 | 1.0 | 0.0 |
3. Sequence Information
Fasta Sequence: SB0065.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Not Available.
* Cleavage Information
4 [sites] cleaved by Calpain 1
Source Reference: [PubMed ID: 9074620] Buki KG, Bauer PI, Kun E, Isolation and identification of a proteinase from calf thymus that cleaves poly(ADP-ribose) polymerase and histone H1. Biochim Biophys Acta. 1997 Mar 7;1338(1):100-6.
Cleavage sites (±10aa)
[Site 1] SAASAPAAVH384-SGPPDKPLSN
His384 
Ser
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ser375 | Ala376 | Ala377 | Ser378 | Ala379 | Pro380 | Ala381 | Ala382 | Val383 | His384 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ser385 | Gly386 | Pro387 | Pro388 | Asp389 | Lys390 | Pro391 | Leu392 | Ser393 | Asn394 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| KQDRIFPPESSTPVGAAAPPSAASAPAAVHSGPPDKPLSNMKILTLGKLSQNKDEVKATI |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Bos taurus | 122.00 | 1 | ADP-ribosyltransferase (NAD+) poly (ADP-ribose) p |
| 2 | N/A | 88.60 | 4 | poly(ADP-ribose) polymerase |
| 3 | Homo sapiens | 88.60 | 2 | poly (ADP-ribose) polymerase family, member 1 |
| 4 | ADP-ribose | 87.00 | 8 | PARP1_MOUSE Poly |
| 5 | Mus musculus | 85.10 | 7 | unnamed protein product |
| 6 | Rattus norvegicus | 66.60 | 1 | ADP-ribosyltransferase 1 |
| 7 | Gallus gallus | 63.50 | 1 | poly (ADP-ribose) polymerase family, member 1 |
| 8 | Tetraodon nigroviridis | 53.50 | 1 | unnamed protein product |
| 9 | Xenopus laevis | 49.30 | 1 | NAD(+) ADP-ribosyltransferase |
| 10 | Danio rerio | 46.60 | 1 | Zgc:110092 |
| 11 | Crimean-Congo hemorrhagic fever virus | 32.00 | 3 | envelope glycoprotein precursor |
Top-ranked sequences
| organism | matching |
|---|---|
| Bos taurus | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#SGPPDKPLSNMKILTLGKLSQNKDEVKATI 414 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#SGPPDKPLSNMKILTLGKLSQNKDEVKATI 414 |
| N/A | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#S-GPPDKPLSNMKILTLGKLSQNKDEVKAT 413 |||||||||+| | | ||| |||||||+#| ||||||||||||||||+||||||| Sbjct 352 KQDRIFPPETSASVAATPPPSTASAPAAVN#SSASADKPLSNMKILTLGKLSRNKDEVKAM 411 Query 414 I 414 | Query 414 I 414 |
| Homo sapiens | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#S-GPPDKPLSNMKILTLGKLSQNKDEVKAT 413 |||||||||+| | | ||| |||||||+#| ||||||||||||||||+||||||| Sbjct 352 KQDRIFPPETSASVAATPPPSTASAPAAVN#SSASADKPLSNMKILTLGKLSRNKDEVKAM 411 Query 414 I 414 | Query 414 I 414 |
| ADP-ribose | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#S-GPPDKPLSNMKILTLGKLSQNKDEVKAT 413 ||||||||||| |+ | | ||| ||+#| | ||||||||||||||||||||| || Sbjct 352 KQDRIFPPESSAPITVHWPLSVTSAPTAVN#SSAPADKPLSNMKILTLGKLSQNKDEAKAV 411 Query 414 I 414 | Query 414 I 414 |
| Mus musculus | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#S-GPPDKPLSNMKILTLGKLSQNKDEVKAT 413 ||||||||||| | | | | ||| ||+#| | ||||||||||||||||||||| || Sbjct 353 KQDRIFPPESSAPAPLALPLSVTSAPTAVN#SSAPADKPLSNMKILTLGKLSQNKDEAKAV 412 Query 414 I 414 | Query 414 I 414 |
| Rattus norvegicus | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#S-GPPDKPLSNMKILTLGKLSQNKDEVKAT 413 ||||+|||||| | | | | ||| ||+#| | ||||||||||||||||||||| || Sbjct 353 KQDRLFPPESSAPAPPAPPVSITSAPTAVN#SSAPADKPLSNMKILTLGKLSQNKDEAKAM 412 Query 414 I 414 | Query 414 I 414 |
| Gallus gallus | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAAVH#SGPPDKPLSNMKILTLGKLSQNKDEVKATI 414 |||||||||++| | +| || |++ #+ | ||||+|||||||||||+||+||| + Sbjct 351 KQDRIFPPEAAT-VNSAPPPPASAPLTETV#TAPQDKPLTNMKILTLGKLSKNKEEVKNIV 409 |
| Tetraodon nigroviridis | Query 356 QDRIFP----PESSTPVGAAAPPSAASAPAAVH#SG-PPDKPLSNMKILTLGKLSQNKDEV 410 |+||+| |++ + | | ||+| | + # | ||||||+ ||++ +||||+|||++ Sbjct 93 QNRIYPKEAAPQTVSAVKAEPLASASSVPVGLP#EGAPPDKPLTGMKLVAVGKLSKNKDDL 152 Query 411 KATI 414 || + Sbjct 153 KAAV 156 |
| Xenopus laevis | Query 355 KQDRIFPPESS-TPVGAAAPPSAAS-APAAVH#SGPPDKPLSNMKILTLGKLSQNKDEVKA 412 + || ||| ++ ||+ +||+| | #+ | |||+| |+| +||||+|||||| Sbjct 351 RHDRAFPPCAAPTPI---SPPAAPEPKPTVEE#TFPEGKPLTNTKVLLIGKLSKNKDEVKT 407 Query 413 TI 414 | Sbjct 408 LI 409 |
| Danio rerio | Query 355 KQDRIFPPESSTPVGAAAPPSAASAPAA---------VH#SGPPDKPLSNMKILTLGKLSQ 405 +|||+|| ++ | || | | ++ || + # | ||||+ ||+| +||||+ Sbjct 344 RQDRVFPKDA--PPAAATPSSGSTTSAATSVSSASKNLT#EAPADKPLTGMKLLAVGKLSK 401 Query 406 NKDEVKATI 414 |||++| + Sbjct 402 NKDDLKKFV 410 |
| Crimean-Congo hemorrhagic fever virus | Query 361 PPESSTPVGAAAPPSAASAPAAVH#SGPPDKP 391 | |+||| | || |+| | #+ || | Sbjct 157 PDETSTPSGTGKESSATSSPHPVS#NRPPTPP 187 |
[Site 2] LQELLSTHLL480-SPWGAEVKVE
Leu480 Ser
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Leu471 | Gln472 | Glu473 | Leu474 | Leu475 | Ser476 | Thr477 | His478 | Leu479 | Leu480 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ser481 | Pro482 | Trp483 | Gly484 | Ala485 | Glu486 | Val487 | Lys488 | Val489 | Glu490 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| ANIRVVSEDFLQDISASTKSLQELLSTHLLSPWGAEVKVEPVEAVGPKGKSGAAPSKKSK |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | cattle, Peptide Partial, 607 aa | 120.00 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 2 | Bos taurus | 119.00 | 1 | ADP-ribosyltransferase (NAD+) poly (ADP-ribose) p |
| 3 | N/A | 102.00 | 5 | poly(ADP-ribose) synthetase |
| 4 | Homo sapiens | 102.00 | 2 | Poly (ADP-ribose) polymerase family, member 1 |
| 5 | ADP-ribose | 96.70 | 8 | PARP1_MOUSE Poly |
| 6 | mice, Peptide Partial, 606 aa | 96.70 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 7 | Mus musculus | 93.20 | 7 | unnamed protein product |
| 8 | Rattus norvegicus | 92.00 | 1 | ADP-ribosyltransferase 1 |
| 9 | Tetraodon nigroviridis | 64.70 | 1 | unnamed protein product |
| 10 | Danio rerio | 62.40 | 1 | Zgc:110092 |
| 11 | chickens, Peptide Partial, 607 aa | 61.20 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 12 | Gallus gallus | 60.80 | 1 | poly (ADP-ribose) polymerase family, member 1 |
| 13 | Xenopus laevis | 51.20 | 1 | NAD(+) ADP-ribosyltransferase |
| 14 | Xenopus, Peptide Partial, 607 aa | 51.20 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
Top-ranked sequences
| organism | matching |
|---|---|
| cattle, Peptide Partial, 607 aa | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 |
| Bos taurus | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 |
| N/A | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 |||||||||||||+|||||||||| |+|#|||||||| |||| | |+|||||| ||||| Sbjct 449 ANIRVVSEDFLQDVSASTKSLQELFLAHIL#SPWGAEVKAEPVEVVAPRGKSGAALSKKSK 508 |
| Homo sapiens | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 |||||||||||||+|||||||||| |+|#|||||||| |||| | |+|||||| ||||| Sbjct 449 ANIRVVSEDFLQDVSASTKSLQELFLAHIL#SPWGAEVKAEPVEVVAPRGKSGAALSKKSK 508 |
| ADP-ribose | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 |||||||||||||+||||||||+||| | |#|||||||| || | | |+||| |||||||| Sbjct 449 ANIRVVSEDFLQDVSASTKSLQDLLSAHSL#SPWGAEVKAEPGEVVAPRGKS-AAPSKKSK 507 |
| mice, Peptide Partial, 606 aa | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 |||||||||||||+||||||||+||| | |#|||||||| || | | |+||| |||||||| Sbjct 42 ANIRVVSEDFLQDVSASTKSLQDLLSAHSL#SPWGAEVKAEPGEVVAPRGKS-AAPSKKSK 100 |
| Mus musculus | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 ||+||| ||||||+|||||||||||| | |#| |||||| || | | ||||| |||||||| Sbjct 450 ANVRVVCEDFLQDVSASTKSLQELLSAHSL#SSWGAEVKAEPGEVVAPKGKS-AAPSKKSK 508 |
| Rattus norvegicus | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKSK 510 ||+||| ||||||+||| |||||||| | |#| ||||||||| | | ||||| |||||||| Sbjct 450 ANVRVVCEDFLQDVSASAKSLQELLSAHSL#SSWGAEVKVEPGEVVVPKGKS-AAPSKKSK 508 |
| Tetraodon nigroviridis | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKS 509 | +|||||||| || +| |+||||+| | +#|||||||||| +| |||| +| + Sbjct 193 AGVRVVSEDFLTDIKSSGKALQELVSLHAI#SPWGAEVKVE-TQAQPMASKSGAMAAKST 250 |
| Danio rerio | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSK 507 | +|||++||| || | |+||||+| | +#|||||||||| | +|| || Sbjct 447 AGVRVVADDFLTDIKESGKALQELISLHAI#SPWGAEVKVEAPAAAAATKSTGAHSSK 503 |
| chickens, Peptide Partial, 607 aa | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKS 509 | +|||||+||+|+ +| | ||||| | +#|||||||| | | | || + || Sbjct 42 AKVRVVSEEFLKDVKSSNKGFQELLSLHAI#SPWGAEVKTEHQE-VAVDGKCSKPANMKS 99 |
| Gallus gallus | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKS 509 | +|||||+||+|+ +| | ||||| | +#|||||||| | | | || + || Sbjct 446 AKVRVVSEEFLKDVKSSNKGFQELLSLHAI#SPWGAEVKTEHQE-VAVDGKCSKPANMKS 503 |
| Xenopus laevis | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKS 509 ||+||||+|||+++ | ||+||||| +#| ||||+| | |+ + || | | Sbjct 446 ANVRVVSDDFLKEVE-SGKSVQELLSQFGI#SSWGAEIKQEAVQPTEKQPSSGPVAGKSS 503 |
| Xenopus, Peptide Partial, 607 aa | Query 451 ANIRVVSEDFLQDISASTKSLQELLSTHLL#SPWGAEVKVEPVEAVGPKGKSGAAPSKKS 509 ||+||||+|||+++ | ||+||||| +#| ||||+| | |+ + || | | Sbjct 42 ANVRVVSDDFLKEV-FSGKSVQELLSQFGI#SSWGAEIKQEAVQPTEKQPSSGPVAGKSS 99 |
[Site 3] EAVGPKGKSG502-AAPSKKSKGP
Gly502 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Glu493 | Ala494 | Val495 | Gly496 | Pro497 | Lys498 | Gly499 | Lys500 | Ser501 | Gly502 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala503 | Ala504 | Pro505 | Ser506 | Lys507 | Lys508 | Ser509 | Lys510 | Gly511 | Pro512 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| ELLSTHLLSPWGAEVKVEPVEAVGPKGKSGAAPSKKSKGPVKEEGTNKSEKRMKLTLKGG |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | cattle, Peptide Partial, 607 aa | 119.00 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 2 | Bos taurus | 118.00 | 1 | ADP-ribosyltransferase (NAD+) poly (ADP-ribose) p |
| 3 | N/A | 98.60 | 4 | poly(ADP-ribose) polymerase |
| 4 | Homo sapiens | 98.20 | 2 | poly (ADP-ribose) polymerase family, member 1 |
| 5 | ADP-ribose | 95.90 | 8 | PREDICTED: similar to Poly |
| 6 | Rattus norvegicus | 91.70 | 1 | ADP-ribosyltransferase 1 |
| 7 | Mus musculus | 90.50 | 7 | poly (ADP-ribose) polymerase family, member 1 |
| 8 | mice, Peptide Partial, 606 aa | 90.10 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 9 | Gallus gallus | 59.70 | 1 | poly (ADP-ribose) polymerase family, member 1 |
| 10 | chickens, Peptide Partial, 607 aa | 59.70 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 11 | Danio rerio | 53.90 | 1 | Zgc:110092 |
| 12 | Xenopus laevis | 52.40 | 1 | NAD(+) ADP-ribosyltransferase |
| 13 | Xenopus, Peptide Partial, 607 aa | 52.40 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 14 | Tetraodon nigroviridis | 50.40 | 1 | unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|---|
| cattle, Peptide Partial, 607 aa | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 |
| Bos taurus | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 |
| N/A | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 || |+||||||||| |||| | |+||||#|| |||||| ||||| |||||||||||||| Sbjct 31 ELFLAHILSPWGAEVKAEPVEVVAPRGKSG#AALSKKSKGQVKEEGINKSEKRMKLTLKGG 90 |
| Homo sapiens | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 || |+||||||||| |||| | |+||||#|| |||||| ||||| |||||||||||||| Sbjct 471 ELFLAHILSPWGAEVKAEPVEVVAPRGKSG#AALSKKSKGQVKEEGINKSEKRMKLTLKGG 530 |
| ADP-ribose | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 +||| |+|+||||||| || | || |||#|| |||||||||||| |||||||||||||| Sbjct 438 DLLSAHVLAPWGAEVKAEPAEPAAPKAKSG#AALSKKSKGPVKEEGVNKSEKRMKLTLKGG 497 |
| Rattus norvegicus | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 |||| | || ||||||||| | | ||||| #||||||||| ||||| |||||||||||||| Sbjct 472 ELLSAHSLSSWGAEVKVEPGEVVVPKGKS-#AAPSKKSKGAVKEEGVNKSEKRMKLTLKGG 530 |
| Mus musculus | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 |||| | || |||||| || | | ||||| #||||||||| ||||| |||||||||||||| Sbjct 472 ELLSAHSLSSWGAEVKAEPGEVVAPKGKS-#AAPSKKSKGAVKEEGVNKSEKRMKLTLKGG 530 |
| mice, Peptide Partial, 606 aa | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 +||| | ||||||||| || | | |+||| #||||||||| |||| |||||||||||||| Sbjct 64 DLLSAHSLSPWGAEVKAEPGEVVAPRGKS-#AAPSKKSKGCFKEEGVNKSEKRMKLTLKGG 122 |
| Gallus gallus | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEE-GTNKSEKRMKLTLKG 531 |||| | +|||||||| | | | || # + || | |||| | +||||+||||+|| Sbjct 468 ELLSLHAISPWGAEVKTEHQE-VAVDGKCS#KPANMKSAGKVKEEQGPSKSEKKMKLTVKG 526 Query 532 G 532 | Query 532 G 532 |
| chickens, Peptide Partial, 607 aa | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEE-GTNKSEKRMKLTLKG 531 |||| | +|||||||| | | | || # + || | |||| | +||||+||||+|| Sbjct 64 ELLSLHAISPWGAEVKTEHQE-VAVDGKCS#KPANMKSAGKVKEEQGPSKSEKKMKLTVKG 122 Query 532 G 532 | Query 532 G 532 |
| Danio rerio | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 ||+| | +|||||||||| | || # | | || | |||| |+||||+||| Sbjct 469 ELISLHAISPWGAEVKVEA-PAAAAATKST#GAHSSKSTGKVKEEEGGSKSKKMKLTVKGG 527 |
| Xenopus laevis | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEE-GTNKSEKRMKLTLKG 531 |||| +| ||||+| | |+ + ||# + || | |||| |+|||||+||||+|| Sbjct 467 ELLSQFGISSWGAEIKQEAVQPTEKQPSSG#PV-AGKSSGKVKEEKGSNKSEKKMKLTVKG 525 Query 532 G 532 | Query 532 G 532 |
| Xenopus, Peptide Partial, 607 aa | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEE-GTNKSEKRMKLTLKG 531 |||| +| ||||+| | |+ + ||# | | | |||| |+|||||+||||+|| Sbjct 63 ELLSQFGISSWGAEIKQEAVQPTEKQPSSG#PVAGKSS-GKVKEEKGSNKSEKKMKLTVKG 121 Query 532 G 532 | Query 532 G 532 |
| Tetraodon nigroviridis | Query 473 ELLSTHLLSPWGAEVKVEPVEAVGPKGKSG#AAPSKKSKGPVKEEGTNKSEKRMKLTLKGG 532 ||+| | +|||||||||| +| |||#| + || | |||+ |+||||+||| Sbjct 215 ELVSLHAISPWGAEVKVE-TQAQPMASKSG#AMAA-KSTGRVKEQEGGGKSKKMKLTVKGG 272 |
[Site 4] QDEEAVKKLT658-VNPGTKSKLP
Thr658 Val
iTraq-117 Signal 1517.9 (
) for KKLTVNPGTK
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Gln649 | Asp650 | Glu651 | Glu652 | Ala653 | Val654 | Lys655 | Lys656 | Leu657 | Thr658 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Val659 | Asn660 | Pro661 | Gly662 | Thr663 | Lys664 | Ser665 | Lys666 | Leu667 | Pro668 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| HSKNFTKHPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQNLIKMIFDVESMKKAM |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Bos taurus | 125.00 | 2 | ADP-ribosyltransferase (NAD+) poly (ADP-ribose) p |
| 2 | cattle, Peptide Partial, 607 aa | 125.00 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 3 | N/A | 122.00 | 9 | poly(ADP-ribose) polymerase |
| 4 | Homo sapiens | 122.00 | 2 | poly (ADP-ribose) polymerase family, member 1 |
| 5 | ADP-ribose | 120.00 | 12 | PREDICTED: similar to Poly |
| 6 | Mus musculus | 115.00 | 11 | unnamed protein product |
| 7 | Rattus norvegicus | 113.00 | 2 | ADP-ribosyltransferase 1 |
| 8 | mice, Peptide Partial, 606 aa | 113.00 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 9 | chickens, Peptide Partial, 607 aa | 113.00 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 10 | Gallus gallus | 113.00 | 1 | poly (ADP-ribose) polymerase family, member 1 |
| 11 | Tetraodon nigroviridis | 105.00 | 2 | unnamed protein product |
| 12 | Xenopus laevis | 103.00 | 3 | NAD(+) ADP-ribosyltransferase |
| 13 | Xenopus, Peptide Partial, 607 aa | 103.00 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 14 | Danio rerio | 101.00 | 2 | Zgc:110092 |
| 15 | Arabidopsis thaliana | 49.30 | 3 | putative poly (ADP-ribose) polymerase |
| 16 | Drosophila melanogaster | 49.30 | 3 | LD31274p |
| 17 | Drosophila, Peptide Partial, 593 aa | 49.30 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT |
| 18 | Oryza sativa (japonica cultivar-group) | 46.20 | 1 | Os07g0413700 |
| 19 | Caenorhabditis elegans | 45.80 | 2 | Poly(ADP-ribose) Metabolism Enzyme family member |
| 20 | Zea mays | 45.10 | 1 | poly(ADP-ribose) polymerase |
| 21 | Caenorhabditis briggsae AF16 | 45.10 | 1 | Hypothetical protein CBG04221 |
| 22 | Schistosoma japonicum | 43.50 | 1 | SJCHGC03992 protein |
| 23 | Oryza sativa Japonica Group | 35.00 | 1 | putative poly(ADP-ribose) polymerase |
| 24 | Rattus norvegicus | 33.50 | 1 | poly (ADP-ribose) polymerase family, member 2 |
| 25 | Dictyostelium discoideum AX4 | 32.70 | 3 | poly(ADP-ribosyl)transferase |
| 26 | Dictyostelium discoideum | 32.70 | 2 | NAD(+) ADP-ribosyltransferase-1B |
| 27 | Dictyostelium discoideum, Peptide Partial, 612 aa | 31.60 | 1 | NAD+:protein(ADP-ribosyl)-transferase, ADPRT {EC |
| 28 | Aspergillus nidulans FGSC A4 | 30.80 | 1 | hypothetical protein AN3129.2 |
| 29 | Emericella nidulans | 30.80 | 1 | poly(ADP)-ribose polymerase |
| 30 | Methanosphaera stadtmanae DSM 3091 | 30.00 | 1 | riboflavin synthase |
Top-ranked sequences
| organism | matching |
|---|---|
| Bos taurus | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |
| cattle, Peptide Partial, 607 aa | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |
| N/A | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||||||#||||||||||||||+||||||||||||||| Sbjct 627 HSKNFTKYPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQDLIKMIFDVESMKKAM 686 |
| Homo sapiens | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||||||#||||||||||||||+||||||||||||||| Sbjct 627 HSKNFTKYPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQDLIKMIFDVESMKKAM 686 |
| ADP-ribose | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |+||||||||||||||||||||||||||||#| ||||||||||||+||||||||||||||| Sbjct 594 HAKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VKPGTKSKLPKPVQDLIKMIFDVESMKKAM 653 |
| Mus musculus | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||||||#| |||||||||||| |+ |||||||||||+ Sbjct 627 HSKNFTKYPKKFYPLEIDYGQDEEAVKKLT#VKPGTKSKLPKPVQELVGMIFDVESMKKAL 686 |
| Rattus norvegicus | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||||| #| |||||||||||| |+ |||||||||||+ Sbjct 627 HSKNFTKYPKKFYPLEIDYGQDEEAVKKLA#VKPGTKSKLPKPVQELVGMIFDVESMKKAL 686 |
| mice, Peptide Partial, 606 aa | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||||||#| |||||||||||| |+ |||||+|||||+ Sbjct 219 HSKNFTKYPKKFYPLEIDYGQDEEAVKKLT#VKPGTKSKLPKPVQELVGMIFDVDSMKKAL 278 |
| chickens, Peptide Partial, 607 aa | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||+|||#|+ |||||| ||+|+||||||||||||||| Sbjct 220 HSKNFTKYPKKFYPLEIDYGQDEEAVRKLT#VSAGTKSKLAKPIQDLIKMIFDVESMKKAM 279 |
| Gallus gallus | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |||||||+||||||||||||||||||+|||#|+ |||||| ||+|+||||||||||||||| Sbjct 624 HSKNFTKYPKKFYPLEIDYGQDEEAVRKLT#VSAGTKSKLAKPIQDLIKMIFDVESMKKAM 683 |
| Tetraodon nigroviridis | Query 630 SKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 | ||||+| ||||||||||||||||||||# |||||| |||| ||||||||||||||| Sbjct 370 SSNFTKYPNKFYPLEIDYGQDEEAVKKLT#ATAGTKSKLAKPVQELIKMIFDVESMKKAM 428 |
| Xenopus laevis | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || ||||+|||||||||||||+|+ ||||+#| |||||| |||| |||+||||||||||| Sbjct 623 HSPNFTKYPKKFYPLEIDYGQEEDVVKKLS#VGAGTKSKLAKPVQELIKLIFDVESMKKAM 682 |
| Xenopus, Peptide Partial, 607 aa | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || ||||+|||||||||||||+|+ ||||+#| |||||| |||| |||+||||||||||| Sbjct 219 HSPNFTKYPKKFYPLEIDYGQEEDVVKKLS#VGAGTKSKLAKPVQELIKLIFDVESMKKAM 278 |
| Danio rerio | Query 630 SKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 | ||||+| ||||||||||||||||||||# + | ||+| ||||+||+|||||||||||| Sbjct 625 SSNFTKYPNKFYPLEIDYGQDEEAVKKLT#QSAGAKSQLEKPVQDLIRMIFDVESMKKAM 683 |
| Arabidopsis thaliana | Query 632 NFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || | | || ||+|||| +++ || # | | | + |+||+||||+ + || Sbjct 629 NFQKQPGKFLPLDIDYGVNKQVAKKEP#FQ--TSSNLAPSLIELMKMLFDVETYRSAM 683 |
| Drosophila melanogaster | Query 632 NFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || | + ||+|| | |++ || +# ||| ||||||+|||++|| | + Sbjct 177 NFVKRTGRMYPIEIQYDDDQKLVKHES#--HFFTSKLEISVQNLIKLIFDIDSMNKTL 231 |
| Drosophila, Peptide Partial, 593 aa | Query 632 NFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || | + ||+|| | |++ || +# ||| ||||||+|||++|| | + Sbjct 213 NFVKRTGRMYPIEIQYDDDQKLVKHES#--HFFTSKLEISVQNLIKLIFDIDSMNKTL 267 |
| Oryza sativa (japonica cultivar-group) | Query 632 NFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || | | |||||+|||| + |+ #++ || || + |+ |+|++|+ + || Sbjct 596 NFQKQPGKFYPLDIDYGV-RQGPKRKD#IDK-MKSSLPPQLLELMNMLFNIETYRAAM 650 |
| Caenorhabditis elegans | Query 629 HSKNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 + |+| | | | +| || + + + #+ ||+|+ ||| |+ ++ |||||+|| |+ Sbjct 552 YRKHFRKMPGMFSYVETDYSEFAQ-ITDTE#ITPGSKTLLPKSVKEVVMSIFDVENMKSAL 610 |
| Zea mays | Query 632 NFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 || | | +||||++||| ++| |+ #++ || | + |+||+|+||+ + || Sbjct 588 NFRKQPGRFYPLDVDYGV-KKAPKRKD#ISE-MKSSLAPQLLELMKMLFNVETYRAAM 642 |
| Caenorhabditis briggsae AF16 | Query 631 KNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 |+| | | | +| || + ++ +|+ #+ ||+++ ||+ |+ ++ |||+|+|| |+ Sbjct 545 KHFRKMPGAFSFVETDYSEFDQ-LKEQD#IIPGSRTLLPRAVKEIVMSIFDIENMKSAL 601 |
| Schistosoma japonicum | Query 633 FTKHPKKFYPLEIDYG-QDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKKAM 688 | || |+ +||||| ||| + # ||+|| || |+++| |+++|++ | Sbjct 134 FIKHKGKYDLVEIDYGVQDENVTIETK#KTEEIKSRLPVAVQVLMQLICDMDNMEQVM 190 |
| Oryza sativa Japonica Group | Query 631 KNFTKHPKKFYPLEIDYGQ-DEEAVKKLT#VNPGT----KSKLPKPVQNLIKMIFDVESMK 685 |+| + +|+ ||+|||+ | | || #|+| | ++|| + + | +| ++ || Sbjct 245 KSFECYARKYTWLEMDYGEADRETNKK--#VSPSTDQIKETKLETRIASFISLICNISMMK 302 Query 686 KAM 688 + | Sbjct 303 QQM 305 |
| Rattus norvegicus | Query 631 KNFTKHPKKFYPLEIDYGQDEEAVKKLT#VNPGTK--SKLPKPVQNLIKMIFDVESMKKAM 688 +|| | | |+ |++|| | | # +| |+| || |+|+| +|++|++ | Sbjct 171 ENFEKVPGKYDMLQMDYAASTEDENKTK#QEETSKPESQLDLRVQELLKLICNVQTMEEMM 230 |
| Dictyostelium discoideum AX4 | Query 644 EIDYGQDEEAVKKLT#VNPGTK-SKLPKPVQNLIKMIFDVESMKKAM 688 | | | | + # || ||| +|||+|+ +|| | |||++ Sbjct 412 EFSSGGDNEVEAPIV#APVNTKPSKLLQPVQSLVNTLFDSELMKKSL 457 |
| Dictyostelium discoideum | Query 644 EIDYGQDEEAVKKLT#VNPGTK-SKLPKPVQNLIKMIFDVESMKKAM 688 | | | | + # || ||| +|||+|+ +|| | |||++ Sbjct 412 EFSSGGDNEVEAPIV#APVNTKPSKLLQPVQSLVNTLFDSELMKKSL 457 |
| Dictyostelium discoideum, Peptide Partial, 612 aa | Query 632 NFTKHPKKFYPLEIDYGQDEE-----------AVKKLT#VNPGTKSKLPKPVQNLIKMIFD 680 || | |+ +|+|| | + | #| + | + || |+|+||| Sbjct 228 NFKKVAGKYDMIELDYSTDSKPKNGASTTATTTTTKKV#VEHKKECSLDERVQELVKLIFD 287 Query 681 VESMKKAM 688 |+ |++ | Sbjct 288 VKMMERTM 295 |
| Aspergillus nidulans FGSC A4 | Query 649 QDEEAVKKLT#VNPGT--KSKLPKPVQNLIKMIFD 680 +||++ || # | |||| ||||+| ||+ Sbjct 288 EDEKSDNKLV#KQPSNIPASKLPAPVQNVISFIFN 321 |
| Emericella nidulans | Query 649 QDEEAVKKLT#VNPGT--KSKLPKPVQNLIKMIFD 680 +||++ || # | |||| ||||+| ||+ Sbjct 288 EDEKSDNKLV#KQPSNIPASKLPAPVQNVISFIFN 321 |
| Methanosphaera stadtmanae DSM 3091 | Query 646 DYGQDEEAVKKLT#VNPGTKSKLPKPVQNLIKMIFDVESMKK 686 | |+||+ +|+| # | |+ ||+||+| |+||| Sbjct 103 DEGKDEKDLKELA#YNRATQH-----ADNLVKMLFKPEAMKK 138 |
* References
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