* Cleavage Information
2 [sites] cleaved by Calpain 2
Source Reference: [PubMed ID: 9485487] Shih M, Lampi KJ, Shearer TR, David LL, Cleavage of beta crystallins during maturation of bovine lens. Mol Vis. 1998 Feb 27;4:4.
Cleavage sites (±10aa)
[Site 1] MAEQH5-STPEQAAAGK
His5 
Ser
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| - | - | - | - | - | Met1 | Ala2 | Glu3 | Gln4 | His5 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ser6 | Thr7 | Pro8 | Glu9 | Gln10 | Ala11 | Ala12 | Ala13 | Gly14 | Lys15 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MAEQHSTPEQAAAGKSHGGLGGSYKVIVYEMENFQ
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
72.00 |
1 |
crystallin, beta B3 |
| 2 |
Ovis aries |
70.10 |
1 |
crystallin, beta B3 |
| 3 |
Mus musculus |
67.80 |
1 |
crystallin, beta B3 |
| 4 |
Homo sapiens |
66.60 |
4 |
betaB3 crystallin |
| 5 |
Rattus norvegicus |
65.50 |
1 |
AF287304_1 betaB3-crystallin |
| 6 |
Rattus norvegicus |
65.10 |
2 |
crystallin, beta B3 |
| 7 |
Tetraodon nigroviridis |
51.20 |
1 |
unnamed protein product |
| 8 |
Gallus gallus |
45.10 |
1 |
crystallin, beta B3 |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|||||#||||||||||||||||||||||||||||||
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|
| Ovis aries |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|||||#| ||||||||||||||||||||||||||||
Sbjct 1 MAEQH#SAPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|
| Mus musculus |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|||||# ||||||||||||||||||| |||+||||
Sbjct 1 MAEQH#GAPEQAAAGKSHGGLGGSYKVTVYELENFQ 35
|
| Homo sapiens |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|||||# ||||||||||| ||||||||+||+||||
Sbjct 1 MAEQH#GAPEQAAAGKSHGDLGGSYKVILYELENFQ 35
|
| Rattus norvegicus |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|||||# |||||| |||||||||||| |||+||||
Sbjct 1 MAEQH#GAPEQAAASKSHGGLGGSYKVTVYELENFQ 35
|
| Rattus norvegicus |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|||||# |||||| |||||||||||| |||+||||
Sbjct 1 MAEQH#GAPEQAAANKSHGGLGGSYKVTVYELENFQ 35
|
| Tetraodon nigroviridis |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
|+|+ #| ||| ||||| || | +|||+++| ||||
Sbjct 33 MSERQ#SAPEQLAAGKSQGGAGATYKVLLFEFENFQ 67
|
| Gallus gallus |
Query 1 MAEQH#STPEQAAAGKSHGGLGGSYKVIVYEMENFQ 35
| || #| ||| |+ | ||+||+ +||+||||
Sbjct 1 MTEQQ#SPPEQMVTGEGAGERGGNYKITIYELENFQ 35
|
[Site 2] MAEQHSTPEQ10-AAAGKSHGGL
Gln10 Ala
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Met1 | Ala2 | Glu3 | Gln4 | His5 | Ser6 | Thr7 | Pro8 | Glu9 | Gln10 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ala11 | Ala12 | Ala13 | Gly14 | Lys15 | Ser16 | His17 | Gly18 | Gly19 | Leu20 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
MAEQHSTPEQAAAGKSHGGLGGSYKVIVYEMENFQGKRCE
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
86.30 |
3 |
crystallin, beta B3 |
| 2 |
Ovis aries |
84.00 |
1 |
crystallin, beta B3 |
| 3 |
Mus musculus |
78.60 |
5 |
crystallin, beta B3 |
| 4 |
Homo sapiens |
78.20 |
6 |
betaB3 crystallin |
| 5 |
Rattus norvegicus |
76.30 |
2 |
AF287304_1 betaB3-crystallin |
| 6 |
Rattus norvegicus |
75.90 |
6 |
CRBB3_RAT Beta-crystallin B3 (Beta-B3 crystallin) |
| 7 |
Gallus gallus |
55.80 |
3 |
crystallin, beta B3 |
| 8 |
Tetraodon nigroviridis |
53.90 |
2 |
unnamed protein product |
| 9 |
Danio rerio |
50.40 |
5 |
beta B3-crystallin |
| 10 |
Canis familiaris |
33.90 |
1 |
PREDICTED: similar to crystallin, beta B1 |
| 11 |
Xenopus laevis |
33.50 |
2 |
hypothetical protein LOC495288 |
| 12 |
N/A |
33.10 |
6 |
A Chain A, Crystal Structure Of Truncated Human B |
| 13 |
Pan troglodytes |
33.10 |
1 |
PREDICTED: crystallin, beta B1 isoform 2 |
| 14 |
synthetic construct |
33.10 |
1 |
crystallin beta B1 |
| 15 |
Xenopus laevis |
32.30 |
2 |
crystallin, beta A1 |
| 16 |
Iguana iguana |
32.30 |
1 |
betaA2-crystallin |
| 17 |
Canis lupus familiaris |
32.00 |
1 |
crystallin, beta A1 |
| 18 |
Oryctolagus cuniculus |
31.20 |
1 |
beta A3-crystallin |
| 19 |
Cynops pyrrhogaster |
31.20 |
1 |
newt beta B1-crystallin |
| 20 |
Rana catesbeiana |
30.80 |
4 |
CRB32_RANCA Beta-crystallin A3-2 gi |
| 21 |
Rana temporaria |
30.80 |
2 |
CRBA1_RANTE Beta-crystallin A1 gi |
| 22 |
Cavia porcellus |
30.80 |
2 |
beta A3-crystallin |
| 23 |
Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form |
30.80 |
1 |
CRBA1_BOVIN Beta-crystallin A3 |
| 24 |
Oryctolagus cuniculus |
30.40 |
1 |
beta A1-crystallin |
| 25 |
Xenopus tropicalis |
30.00 |
1 |
crystallin, beta B1 |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
||||||||||#||||||||||||||||||||||||||||||
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
|
| Ovis aries |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
|||||| |||#||||||||||||||||||||||||||||||
Sbjct 1 MAEQHSAPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
|
| Mus musculus |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
||||| |||#|||||||||||||||| |||+|||||||||
Sbjct 1 MAEQHGAPEQ#AAAGKSHGGLGGSYKVTVYELENFQGKRCE 40
|
| Homo sapiens |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
||||| |||#|||||||| ||||||||+||+|||||||||
Sbjct 1 MAEQHGAPEQ#AAAGKSHGDLGGSYKVILYELENFQGKRCE 40
|
| Rattus norvegicus |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
||||| |||#||| |||||||||||| |||+|||||||||
Sbjct 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40
|
| Rattus norvegicus |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
||||| |||#||| |||||||||||| |||+|||||||||
Sbjct 1 MAEQHGAPEQ#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40
|
| Gallus gallus |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
| || | |||# |+ | ||+||+ +||+|||||+|||
Sbjct 1 MTEQQSPPEQ#MVTGEGAGERGGNYKITIYELENFQGRRCE 40
|
| Tetraodon nigroviridis |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
|+|+ | |||# ||||| || | +|||+++| ||||| + |
Sbjct 33 MSERQSAPEQ#LAAGKSQGGAGATYKVLLFEFENFQGCKAE 72
|
| Danio rerio |
Query 1 MAEQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
|++| |+|# |||| || | ||| +|| |||+||+ |
Sbjct 1 MSDQQGVPDQ#QVAGKSQGGAGAIYKVSLYEFENFRGKKLE 40
|
| Canis familiaris |
Query 22 GSYKVIVYEMENFQGKRCE 40
||||++|+| |||||+| |
Sbjct 53 GSYKLVVFEQENFQGRRVE 71
|
| Xenopus laevis |
Query 22 GSYKVIVYEMENFQGKRCE 40
| +|+ |+| |||||||||
Sbjct 10 GQWKMTVWEEENFQGKRCE 28
|
| N/A |
Query 22 GSYKVIVYEMENFQGKRCE 40
|+|+++|+|+|||||+| |
Sbjct 15 GNYRLVVFELENFQGRRAE 33
|
| Pan troglodytes |
Query 22 GSYKVIVYEMENFQGKRCE 40
|+|+++|+|+|||||+| |
Sbjct 57 GNYRLVVFELENFQGRRAE 75
|
| synthetic construct |
Query 22 GSYKVIVYEMENFQGKRCE 40
|+|+++|+|+|||||+| |
Sbjct 57 GNYRLVVFELENFQGRRAE 75
|
| Xenopus laevis |
Query 22 GSYKVIVYEMENFQGKRCE 40
||+|+ ||+ ||||||| |
Sbjct 28 GSWKITVYDQENFQGKRME 46
|
| Iguana iguana |
Query 22 GSYKVIVYEMENFQGKRCE 40
| +|+ |+| |||||+|||
Sbjct 3 GQFKITVWEEENFQGERCE 21
|
| Canis lupus familiaris |
Query 19 GLGGSYKVIVYEMENFQGKRCE 40
| | +|+ +|+ ||||||| |
Sbjct 26 GSAGPWKITIYDQENFQGKRME 47
|
| Oryctolagus cuniculus |
Query 3 EQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
| | + #| | || +|+ +|+ ||||||| |
Sbjct 11 ETLPTTKM#AQTNPMPGSLG-PWKITIYDQENFQGKRME 47
|
| Cynops pyrrhogaster |
Query 22 GSYKVIVYEMENFQGKRCE 40
|+||+|++| |||||+ |
Sbjct 46 GTYKIIIFEQENFQGRHLE 64
|
| Rana catesbeiana |
Query 22 GSYKVIVYEMENFQGKRCE 40
| +|+ ||+ ||||||| |
Sbjct 29 GPWKITVYDQENFQGKRME 47
|
| Rana temporaria |
Query 22 GSYKVIVYEMENFQGKRCE 40
| +|+ ||+ ||||||| |
Sbjct 12 GPWKITVYDQENFQGKRME 30
|
| Cavia porcellus |
Query 3 EQHSTPEQ#AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
| | + #| | || +|+ +|+ ||||||| |
Sbjct 11 ETLPTTKM#AQTNPMPGSLG-PWKITIYDQENFQGKRME 47
|
| Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form |
Query 19 GLGGSYKVIVYEMENFQGKRCE 40
| | +|+ +|+ ||||||| |
Sbjct 26 GSVGPWKITIYDQENFQGKRME 47
|
| Oryctolagus cuniculus |
Query 22 GSYKVIVYEMENFQGKRCE 40
| +|+ +|+ ||||||| |
Sbjct 12 GPWKITIYDQENFQGKRME 30
|
| Xenopus tropicalis |
Query 11 AAAGKSHGGLGGSYKVIVYEMENFQGKRCE 40
| ++ + ||+|++++| |||||+ |
Sbjct 24 AKPTRTGDPMMGSFKIVLFEQENFQGRHVE 53
|
* References
[PubMed ID: 19206113] Diemer H, Atmanene C, Sanglier S, Morrissey B, Van Dorsselaer A, Downard KM, Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS). J Mass Spectrom. 2009 May;44(5):803-12. doi: 10.1002/jms.1560.
» More (3) « Hide Below
[PubMed ID: 19393038] ... Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marcais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL, A whole-genome assembly of the domestic cow, Bos taurus. Genome Biol. 2009;10(4):R42. doi: 10.1186/gb-2009-10-4-r42. Epub 2009 Apr 24.
[PubMed ID: 12803541] ... Yan H, Willis AC, Harding JJ, Gamma III-crystallin is the primary target of glycation in the bovine lens incubated under physiological conditions. Biochem J. 2003 Sep 15;374(Pt 3):677-85.
[PubMed ID: 6698025] ... Berbers GA, Hoekman WA, Bloemendal H, de Jong WW, Kleinschmidt T, Braunitzer G, Homology between the primary structures of the major bovine beta-crystallin chains. Eur J Biochem. 1984 Mar 15;139(3):467-79.
