* Cleavage Information
2 [sites] cleaved by Calpain 2
Source Reference: [PubMed ID: 9485487] Shih M, Lampi KJ, Shearer TR, David LL, Cleavage of beta crystallins during maturation of bovine lens. Mol Vis. 1998 Feb 27;4:4.
Cleavage sites (±10aa)
[Site 1] ETQTVQQELK11-SLPTTKMAQT
Lys11 
Ser
iTraq-117 Signal 423.5 (
) for ETQTVQQELK
iTraq-117 Signal 565.0 () for SLPTTKMAQT
 |
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Glu2 | Thr3 | Gln4 | Thr5 | Val6 | Gln7 | Gln8 | Glu9 | Leu10 | Lys11 |
 |
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Ser12 | Leu13 | Pro14 | Thr15 | Thr16 | Lys17 | Met18 | Ala19 | Gln20 | Thr21 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
METQTVQQELKSLPTTKMAQTNPMPGSVGPWKITIYDQENF
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
87.40 |
1 |
crystallin, beta A1 |
| 2 |
N/A |
85.90 |
4 |
beta crystallin subunit beta-A3 |
| 3 |
Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form |
85.90 |
1 |
CRBA1_BOVIN Beta-crystallin A3 |
| 4 |
Canis lupus familiaris |
83.60 |
1 |
crystallin, beta A1 |
| 5 |
Oryctolagus cuniculus |
83.60 |
1 |
beta A3-crystallin |
| 6 |
Cavia porcellus |
82.80 |
2 |
beta A3-crystallin |
| 7 |
Rattus norvegicus |
82.00 |
2 |
PREDICTED: similar to Beta crystallin A3 |
| 8 |
Mus musculus |
82.00 |
2 |
crystallin, beta A1 |
| 9 |
Homo sapiens |
77.00 |
1 |
crystallin, beta A3 |
| 10 |
Rana catesbeiana |
55.80 |
4 |
CRB32_RANCA Beta-crystallin A3-2 gi |
| 11 |
Oryctolagus cuniculus |
55.50 |
1 |
beta A1-crystallin |
| 12 |
Tetraodon nigroviridis |
49.30 |
1 |
unnamed protein product |
| 13 |
Gallus gallus |
48.10 |
1 |
crystallin, beta A1 |
| 14 |
Xenopus laevis |
47.80 |
1 |
crystallin, beta A1 |
| 15 |
Rana temporaria |
47.00 |
2 |
unnamed protein product |
| 16 |
Xenopus laevis |
42.40 |
1 |
hypothetical protein LOC494645 |
| 17 |
Danio rerio |
41.60 |
1 |
crystallin, beta A1 |
| 18 |
rats, Sprague-Dawley, lens, Peptide Partial, 20 aa |
41.60 |
1 |
beta-crystallin isoform A3 {internal fragment} |
| 19 |
rats, Sprague-Dawley, lens, Peptide Partial, 15 aa |
29.60 |
1 |
beta-crystallin isoform A3/A1 {internal fragment} |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|||||||||||#||||||||||||||||||||||||||||||
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|
| N/A |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
||||||||||+#||||||||||||||||||||||||||||||
Sbjct 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|
| Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
||||||||||+#||||||||||||||||||||||||||||||
Sbjct 1 METQTVQQELE#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|
| Canis lupus familiaris |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
||||||||||+#+||||||||||||||| |||||||||||||
Sbjct 1 METQTVQQELE#TLPTTKMAQTNPMPGSAGPWKITIYDQENF 41
|
| Oryctolagus cuniculus |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
||||||||||+#+|||||||||||||||+|||||||||||||
Sbjct 1 METQTVQQELE#TLPTTKMAQTNPMPGSLGPWKITIYDQENF 41
|
| Cavia porcellus |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|||||||+||+#+|||||||||||||||+|||||||||||||
Sbjct 1 METQTVQRELE#TLPTTKMAQTNPMPGSLGPWKITIYDQENF 41
|
| Rattus norvegicus |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|||||||+||+#+|||||||||||||||+|||||||||||||
Sbjct 1 METQTVQRELE#TLPTTKMAQTNPMPGSMGPWKITIYDQENF 41
|
| Mus musculus |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|||||||+||+#+|||||||||||||||+|||||||||||||
Sbjct 1 METQTVQRELE#TLPTTKMAQTNPMPGSLGPWKITIYDQENF 41
|
| Homo sapiens |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|||| ||||+#+||||||||||| |||+|||||||||||||
Sbjct 1 METQAEQQELE#TLPTTKMAQTNPTPGSLGPWKITIYDQENF 41
|
| Rana catesbeiana |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|| +| | +# + + |||| ||+| +||||||+||||||
Sbjct 1 MEIPAIQTERE#DITSEKMAQINPLPVPLGPWKITVYDQENF 41
|
| Oryctolagus cuniculus |
Query 18 MAQTNPMPGSVGPWKITIYDQENF 41
||||||||||+|||||||||||||
Sbjct 1 MAQTNPMPGSLGPWKITIYDQENF 24
|
| Tetraodon nigroviridis |
Query 16 TKMAQTNPMPGSVGPWKITIYDQENF 41
|||||||||| +||||||+||||+|
Sbjct 1 TKMAQTNPMP--MGPWKITVYDQEHF 24
|
| Gallus gallus |
Query 18 MAQTNPMPGSVGPWKITIYDQENF 41
||||||+| +||||||+||||||
Sbjct 1 MAQTNPLPVPMGPWKITVYDQENF 24
|
| Xenopus laevis |
Query 1 METQTVQQELK#SLPTTKMAQTNPMPGSVGPWKITIYDQENF 41
|| || | +#+ | |||+|||+ +| ||||+||||||
Sbjct 1 MEISPVQTERE#NF-TKKMAETNPLSMRMGSWKITVYDQENF 40
|
| Rana temporaria |
Query 18 MAQTNPMPGSVGPWKITIYDQENF 41
||| ||+| +||||||+||||||
Sbjct 1 MAQINPLPVPLGPWKITVYDQENF 24
|
| Xenopus laevis |
Query 18 MAQTNPMPGSVGPWKITIYDQENF 41
||+|||+ +| ||||+||||||
Sbjct 1 MAETNPLSMHIGSWKITVYDQENF 24
|
| Danio rerio |
Query 18 MAQTNPMPGSVGPWKITIYDQENF 41
|| ||||| +||||+|+||||||
Sbjct 1 MALTNPMP--LGPWKLTVYDQENF 22
|
| rats, Sprague-Dawley, lens, Peptide Partial, 20 aa |
Query 12 SLPTTKMAQTNPMPGSVGPW 31
+|||||||||||||||+|||
Sbjct 1 TLPTTKMAQTNPMPGSMGPW 20
|
| rats, Sprague-Dawley, lens, Peptide Partial, 15 aa |
Query 22 NPMPGSVGPWKITIY 36
+|||||+||||| |
Sbjct 1 DPMPGSMGPWKIIXY 15
|
[Site 2] QELKSLPTTK17-MAQTNPMPGS
Lys17 Met
|
| P10 | P9 | P8 | P7 | P6 |
P5 | P4 | P3 | P2 | P1 |
| Gln8 | Glu9 | Leu10 | Lys11 | Ser12 | Leu13 | Pro14 | Thr15 | Thr16 | Lys17 |
|
| P1' | P2' | P3' | P4' | P5' |
P6' | P7' | P8' | P9' | P10' |
| Met18 | Ala19 | Gln20 | Thr21 | Asn22 | Pro23 | Met24 | Pro25 | Gly26 | Ser27 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
|
METQTVQQELKSLPTTKMAQTNPMPGSVGPWKITIYDQENFQGKRME
|
Summary
| # |
organism |
max score |
hits |
top seq |
| 1 |
Bos taurus |
99.80 |
3 |
crystallin, beta A1 |
| 2 |
N/A |
98.20 |
7 |
beta crystallin subunit beta-A3 |
| 3 |
Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form |
98.20 |
1 |
CRBA1_BOVIN Beta-crystallin A3 |
| 4 |
Oryctolagus cuniculus |
95.90 |
1 |
beta A3-crystallin |
| 5 |
Canis lupus familiaris |
95.50 |
2 |
crystallin, beta A1 |
| 6 |
Cavia porcellus |
94.70 |
2 |
beta A3-crystallin |
| 7 |
Mus musculus |
94.40 |
7 |
crystallin, beta A1 |
| 8 |
Rattus norvegicus |
94.00 |
6 |
PREDICTED: similar to Beta crystallin A3 |
| 9 |
Homo sapiens |
89.00 |
3 |
crystallin, beta A3 |
| 10 |
Rana catesbeiana |
67.00 |
4 |
CRB32_RANCA Beta-crystallin A3-2 gi |
| 11 |
Oryctolagus cuniculus |
67.00 |
1 |
beta A1-crystallin |
| 12 |
Xenopus laevis |
59.70 |
2 |
crystallin, beta A1 |
| 13 |
Tetraodon nigroviridis |
59.30 |
5 |
unnamed protein product |
| 14 |
Gallus gallus |
59.30 |
3 |
crystallin, beta A1 |
| 15 |
Rana temporaria |
58.50 |
2 |
unnamed protein product |
| 16 |
Danio rerio |
54.70 |
5 |
crystallin, beta A1 |
| 17 |
Xenopus laevis |
54.30 |
2 |
hypothetical protein LOC494645 |
| 18 |
rats, Sprague-Dawley, lens, Peptide Partial, 20 aa |
42.00 |
1 |
beta-crystallin isoform A3 {internal fragment} |
| 19 |
Canis familiaris |
34.30 |
1 |
PREDICTED: similar to crystallin, beta B1 |
| 20 |
Pan troglodytes |
33.90 |
1 |
PREDICTED: crystallin, beta A4 |
| 21 |
Cynops pyrrhogaster |
33.10 |
1 |
newt beta B1-crystallin |
| 22 |
Rattus norvegicus |
31.60 |
2 |
AF287304_1 betaB3-crystallin |
| 23 |
Xenopus tropicalis |
31.20 |
1 |
crystallin, beta B1 |
| 24 |
Rhodoferax ferrireducens T118 |
30.40 |
1 |
histidine kinase |
| 25 |
Dicentrarchus labrax |
30.40 |
1 |
crystallin beta B1 |
| 26 |
Ovis aries |
30.40 |
1 |
crystallin, beta B3 |
| 27 |
Iguana iguana |
30.00 |
1 |
betaA2-crystallin |
| 28 |
rats, Sprague-Dawley, lens, Peptide Partial, 15 aa |
29.60 |
1 |
beta-crystallin isoform A3/A1 {internal fragment} |
| 29 |
rats, Sprague-Dawley, lens, Peptide Partial, 24 aa |
28.50 |
1 |
beta-crystallin isoform A4 {N-terminal} |
Top-ranked sequences
| organism | matching |
|---|
| Bos taurus |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|||||||||||||||||#||||||||||||||||||||||||||||||
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|
| N/A |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||||||||||+||||||#||||||||||||||||||||||||||||||
Sbjct 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|
| Contains: Beta-crystallin A3, isoform A1, Delta4 form; Beta-crystallin A3, isoform A1, Delta7 form; Beta-crystallin A3, isoform A1, Delta8 form |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||||||||||+||||||#||||||||||||||||||||||||||||||
Sbjct 1 METQTVQQELESLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|
| Oryctolagus cuniculus |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||||||||||++|||||#||||||||||+|||||||||||||||||||
Sbjct 1 METQTVQQELETLPTTK#MAQTNPMPGSLGPWKITIYDQENFQGKRME 47
|
| Canis lupus familiaris |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||||||||||++|||||#|||||||||| |||||||||||||||||||
Sbjct 1 METQTVQQELETLPTTK#MAQTNPMPGSAGPWKITIYDQENFQGKRME 47
|
| Cavia porcellus |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|||||||+||++|||||#||||||||||+|||||||||||||||||||
Sbjct 1 METQTVQRELETLPTTK#MAQTNPMPGSLGPWKITIYDQENFQGKRME 47
|
| Mus musculus |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|||||||+||++|||||#||||||||||+|||||||||||||||||||
Sbjct 1 METQTVQRELETLPTTK#MAQTNPMPGSLGPWKITIYDQENFQGKRME 47
|
| Rattus norvegicus |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|||||||+||++|||||#||||||||||+|||||||||||||||||||
Sbjct 1 METQTVQRELETLPTTK#MAQTNPMPGSMGPWKITIYDQENFQGKRME 47
|
| Homo sapiens |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|||| ||||++|||||#|||||| |||+|||||||||||||||||||
Sbjct 1 METQAEQQELETLPTTK#MAQTNPTPGSLGPWKITIYDQENFQGKRME 47
|
| Rana catesbeiana |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|| +| | + + + |#||| ||+| +||||||+||||||||||||
Sbjct 1 MEIPAIQTEREDITSEK#MAQINPLPVPLGPWKITVYDQENFQGKRME 47
|
| Oryctolagus cuniculus |
Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||||||||||+|||||||||||||||||||
Sbjct 1 MAQTNPMPGSLGPWKITIYDQENFQGKRME 30
|
| Xenopus laevis |
Query 1 METQTVQQELKSLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|| || | ++ | |#||+|||+ +| ||||+||||||||||||
Sbjct 1 MEISPVQTERENF-TKK#MAETNPLSMRMGSWKITVYDQENFQGKRME 46
|
| Tetraodon nigroviridis |
Query 16 TK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||#|||||||| +||||||+||||+|||+|||
Sbjct 1 TK#MAQTNPMP--MGPWKITVYDQEHFQGRRME 30
|
| Gallus gallus |
Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||||||+| +||||||+||||||||||||
Sbjct 1 MAQTNPLPVPMGPWKITVYDQENFQGKRME 30
|
| Rana temporaria |
Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||| ||+| +||||||+||||||||||||
Sbjct 1 MAQINPLPVPLGPWKITVYDQENFQGKRME 30
|
| Danio rerio |
Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
|| ||||| +||||+|+||||||||||||
Sbjct 1 MALTNPMP--LGPWKLTVYDQENFQGKRME 28
|
| Xenopus laevis |
Query 18 MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
||+|||+ +| ||||+||||||||||||
Sbjct 1 MAETNPLSMHIGSWKITVYDQENFQGKRME 30
|
| rats, Sprague-Dawley, lens, Peptide Partial, 20 aa |
Query 12 SLPTTK#MAQTNPMPGSVGPW 31
+|||||#||||||||||+|||
Sbjct 1 TLPTTK#MAQTNPMPGSMGPW 20
|
| Canis familiaris |
Query 12 SLPTTK#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
++||||# | | +|+ +++||||||+|+|
Sbjct 41 TVPTTK#AGDLPP-----GSYKLVVFEQENFQGRRVE 71
|
| Pan troglodytes |
Query 27 SVGPWKITIYDQENFQGKRME 47
| ||||+ ++|++ |||+| |
Sbjct 8 SAGPWKMVVWDEDGFQGRRHE 28
|
| Cynops pyrrhogaster |
Query 27 SVGPWKITIYDQENFQGKRME 47
||| +|| |++||||||+ +|
Sbjct 44 SVGTYKIIIFEQENFQGRHLE 64
|
| Rattus norvegicus |
Query 17 K#MAQTNPMPGSVGPWKITIYDQENFQGKRME 47
+# | + | | +|+|+|+ ||||||| |
Sbjct 10 Q#AAASKSHGGLGGSYKVTVYELENFQGKRCE 40
|
| Xenopus tropicalis |
Query 13 LPTTK#MAQT-NPMPGSVGPWKITIYDQENFQGKRME 47
+|+ |# +| +|| || || +++||||||+ +|
Sbjct 21 VPSAK#PTRTGDPMMGSF---KIVLFEQENFQGRHVE 53
|
| Rhodoferax ferrireducens T118 |
Query 18 MAQTNPMPGSVGPWKITIYD-QENFQG 43
+||| |+||| || ||++ || +|
Sbjct 280 LAQTQPLPGS--PWSITVFSLQEQVRG 304
|
| Dicentrarchus labrax |
Query 25 PGSVGPWKITIYDQENFQGKRME 47
|| +| ++| ++|||||||+ +|
Sbjct 35 PG-MGSYRIMMFDQENFQGRMIE 56
|
| Ovis aries |
Query 29 GPWKITIYDQENFQGKRME 47
| +|+ +|+ ||||||| |
Sbjct 22 GSYKVIVYEMENFQGKRCE 40
|
| Iguana iguana |
Query 27 SVGPWKITIYDQENFQGKRME 47
++| +|||++++|||||+| |
Sbjct 1 TLGQFKITVWEEENFQGERCE 21
|
| rats, Sprague-Dawley, lens, Peptide Partial, 15 aa |
Query 22 NPMPGSVGPWKITIY 36
+|||||+||||| |
Sbjct 1 DPMPGSMGPWKIIXY 15
|
| rats, Sprague-Dawley, lens, Peptide Partial, 24 aa |
Query 27 SVGPWKITIYDQENFQG 43
| | |++ ++|+| |||
Sbjct 8 SAGHWRVVVWDEEGFQG 24
|
* References
[PubMed ID: 19393038] Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marcais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL, A whole-genome assembly of the domestic cow, Bos taurus. Genome Biol. 2009;10(4):R42. doi: 10.1186/gb-2009-10-4-r42. Epub 2009 Apr 24.
» More (4) « Hide Below
[PubMed ID: 17651443] ... Jobby MK, Sharma Y, Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins. FEBS J. 2007 Aug;274(16):4135-47. Epub 2007 Jul 25.
[PubMed ID: 6527379] ... Quax-Jeuken Y, Janssen C, Quax W, van den Heuvel R, Bloemendal H, Bovine beta-crystallin complementary DNA clones. Alternating proline/alanine sequence of beta B1 subunit originates from a repetitive DNA sequence. J Mol Biol. 1984 Dec 15;180(3):457-72.
[PubMed ID: 6547897] ... Gorin MB, Horwitz J, Cloning and characterization of a cow beta crystallin cDNA. Curr Eye Res. 1984 Jul;3(7):939-48.
[PubMed ID: 6698025] ... Berbers GA, Hoekman WA, Bloemendal H, de Jong WW, Kleinschmidt T, Braunitzer G, Homology between the primary structures of the major bovine beta-crystallin chains. Eur J Biochem. 1984 Mar 15;139(3):467-79.
