SB0070 : IGFBP4, Insulin-like growth factor binding protein 4
[ CaMP Format ]
* Basic Information
| Organism | Homo sapiens (human) |
| Protein Names | insulin-like growth factor-binding protein 4 precursor [Homo sapiens]; insulin-like growth factor-binding protein 4 precursor; insulin-like growth factor-binding protein 4; IGF-binding protein 4; IBP-4; Insulin-like growth factor-binding protein 4; IGFBP-4 |
| Gene Names | IGFBP4; IBP4; insulin-like growth factor binding protein 4 |
| Gene Locus | 17q21.2; chromosome 17 |
| GO Function | Not available |
* Information From OMIM
Description: Insulin-like growth factor (see IGF1; OMIM:147440)-binding proteins (IGFBPs), such as IGFBP4, are involved in the systemic and local regulation of IGF activity. IGFBPs contain 3 structurally distinct domains each comprising approximately one-third of the molecule. The N-terminal domain 1 and the C-terminal domain 3 of IGFBPs show moderate to high levels of sequence identity, including 12 and 6 invariant cysteines in domains 1 and 3, respectively (IGFBP6 (OMIM:146735) contains 10 cysteines in domain 1), and are thought to be the IGF-binding domains. Domain 2 is defined primarily by a lack of sequence identity among the IGFBPs and by a lack of cysteines, though it does contain 2 cysteines in IGFBP4. Domain 3 is homologous to the thyroglobulin type I repeat unit (Kiefer et al., 1992).
Function: Shimasaki et al. (1990) stated that IGFBP4 acts as an inhibitor of IGF-induced bone cell proliferation.
* Structure Information
1. Primary Information
Length: 258 aa
Average Mass: 27.934 kDa
Monoisotopic Mass: 27.915 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| --- cleavage 23 --- | ||||
| Thyroglobulin type-1 repeat 1. | 68 | 75 | 22.0 | 0.1 |
| --- cleavage 107 --- | ||||
| Insulin-like growth factor binding protein 1. | 130 | 138 | 36.0 | 0.1 |
| --- cleavage 143 --- | ||||
| --- cleavage 159 --- | ||||
| Insulin-like growth factor binding protein 2. | 212 | 228 | 11.0 | 1.2 |
3. Sequence Information
Fasta Sequence: SB0070.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
* Cleavage Information
4 [sites] cleaved by Calpain 1
Source Reference: [PubMed ID: 15843151] Ghosh M, Shanker S, Siwanowicz I, Mann K, Machleidt W, Holak TA, Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain. Biol Chem. 2005 Jan;386(1):85-93.
Cleavage sites (±10aa)
[Site 1] AGPGPSLGDE23-AIHCPPCSEE
Glu23 
Ala
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ala14 | Gly15 | Pro16 | Gly17 | Pro18 | Ser19 | Leu20 | Gly21 | Asp22 | Glu23 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala24 | Ile25 | His26 | Cys27 | Pro28 | Pro29 | Cys30 | Ser31 | Glu32 | Glu33 |
Sequence conservation (by blast)
Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| MLPLCLVAALLLAAGPGPSLGDEAIHCPPCSEEKLARCRPPVGCEELVREPGC |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | Homo sapiens | 103.00 | 7 | insulin-like growth factor binding protein 4 prec |
| 2 | synthetic construct | 103.00 | 6 | insulin-like growth factor binding protein 4 |
| 3 | Canis familiaris | 102.00 | 2 | PREDICTED: similar to Insulin-like growth factor |
| 4 | N/A | 101.00 | 8 | insulin-like growth factor binding protein-4 |
| 5 | Bos taurus | 100.00 | 4 | insulin-like growth factor binding protein 4 |
| 6 | Mus musculus | 95.50 | 12 | insulin-like growth factor binding protein-4 |
| 7 | Rattus norvegicus | 81.60 | 5 | insulin-like growth factor binding protein 4 |
| 8 | Equus caballus | 67.80 | 1 | insulin-like growth factor binding protein-4 |
| 9 | Ovis aries | 67.40 | 1 | IBP4_SHEEP Insulin-like growth factor-binding pro |
| 10 | Tetraodon nigroviridis | 55.10 | 3 | unnamed protein product |
| 11 | Gallus gallus | 50.80 | 3 | insulin-like growth factor binding protein 4 |
| 12 | Danio rerio | 42.70 | 4 | insulin-like growth factor binding protein 1 |
| 13 | human, TE85 osteosarcoma cells, Peptide Partial, 21 aa | 42.70 | 1 | insulin-like growth factor binding protein 4, IGF |
| 14 | Sparus aurata | 38.50 | 1 | AF377998_1 insulin-like growth factor binding pro |
| 15 | Xenopus laevis | 37.00 | 3 | insulin-like growth factor binding protein 5 |
| 16 | Xenopus tropicalis | 36.20 | 2 | insulin-like growth factor binding protein 5 |
| 17 | Oncorhynchus mykiss | 34.70 | 3 | insulin-like growth factor binding protein 1 |
| 18 | Oncorhynchus tshawytscha | 34.70 | 1 | insulin-like growth factor binding protein-1 |
| 19 | Sus scrofa | 33.50 | 1 | insulin-like growth factor binding protein 5 |
| 20 | Pseudomonas syringae pv. tomato str. DC3000 | 32.00 | 1 | Rhs element Vgr protein |
| 21 | Paracoccus denitrificans PD1222 | 30.80 | 1 | Binding-protein-dependent transport systems inner |
| 22 | Papio anubis | 30.80 | 1 | insulin-like growth factor binding protein-1 |
| 23 | swine, serum, Peptide Partial, 16 aa | 30.00 | 1 | insulin-like growth factor-binding protein-4, IGF |
Top-ranked sequences
| organism | matching |
|---|---|
| Homo sapiens | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| synthetic construct | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| Canis familiaris | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |||||||||||||||||||||||#||||| |||||||||||||||||||||||| Sbjct 194 MLPLCLVAALLLAAGPGPSLGDE#AIHCPSCSEEKLARCRPPVGCEELVREPGC 246 |
| N/A | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |||||||||||||||||||||||#||||||||||||||||||||||||||| || Sbjct 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREAGC 53 |
| Bos taurus | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 || |||+||||||||||||||||#|||||||||||||||||||||||||||||| Sbjct 1 MLSLCLMAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| Mus musculus | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 ||| ||||||| ||| ||||||#|||||||||||||||||||||||||||||| Sbjct 1 MLPCSLVAALLLTAGPRPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| Rattus norvegicus | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 ||| ||||||||||| ||||||#|||||||||||||||||||||||||||||| Sbjct 1 MLPFGLVAALLLAAGPRPSLGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| Equus caballus | Query 22 DE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 ||#|||||||||||||||||||||||||||||| Query 22 DE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| Ovis aries | Query 22 DE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 ||#|||||||||||||||||||||||||||||| Query 22 DE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 |
| Tetraodon nigroviridis | Query 20 LGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 | |+#|| || ||||+||||| | |||| |||||| Sbjct 29 LSDQ#AIRCPVCSEERLARCRLPEGCEETVREPGC 62 |
| Gallus gallus | Query 22 DE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 +|#| |||||||+||||+ | || | |||||| Sbjct 27 EE#ATQCPPCSEERLARCKAPQGCTESVREPGC 58 |
| Danio rerio | Query 1 MLPLCLVAAL-LLAAGPG----PSLGDE#AIHCPPCSEEKLARCRPPV--GCEELVREPGC 53 +| ||| | | || | +| |# | | ||| |+|| | | | ||||++||||| Sbjct 5 LLNFFWVAAFSALLAAPGLRASPVVGQE#PIRCAPCSPERLAEC-PAVDAGCEEVLREPGC 63 |
| human, TE85 osteosarcoma cells, Peptide Partial, 21 aa | Query 22 DE#AIHCPPCSEEKLARCRPPV 42 ||#|||||| |||||||||||| Sbjct 1 DE#AIHCPPXSEEKLARCRPPV 21 |
| Sparus aurata | Query 10 LLLAAGPGPSLGDE#AIHCPPCSEEKLARC-RPPVGCEELVREPGC 53 | + || |||| #| || |+ |+|| | + | |+||| || Sbjct 11 LTYVSLPGHSLGDL#AFRCPSCTAERLAACPKVTAMCPEIVRELGC 55 |
| Xenopus laevis | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKLARCRP-PVGCEELVREPGC 53 +||+||+ + | | +|| # +|| || ++ ++ | | |||| |||+|||| Sbjct 4 LLPMCLLL-VSLCLGQCQALGS-#FVHCEPCDDKAMSMCPPTPVGC-ELVKEPGC 54 |
| Xenopus tropicalis | Query 6 LVAALLLAA---GPGPSLGDE#AIHCPPCSEEKLARCRP-PVGCEELVREPGC 53 || |||| + | +|| # +|| || ++ ++ | | |||| |||+|||| Sbjct 4 LVPALLLVSLCLGQCQALG-S#FVHCEPCDDKAMSMCPPTPVGC-ELVKEPGC 53 |
| Oncorhynchus mykiss | Query 14 AGPGPSLGDE#AIHCPPCSEEKLARCRPPVG--CEELVREPGC 53 | | +| |# | | ||++||| | | + |++++||||| Sbjct 23 AQSSPVVGPE#PIRCAPCTQEKLDEC-PAISPDCKQVLREPGC 63 |
| Oncorhynchus tshawytscha | Query 14 AGPGPSLGDE#AIHCPPCSEEKLARCRPPVG--CEELVREPGC 53 | | +| |# | | ||++||| | | + |++++||||| Sbjct 23 AQSSPVVGPE#PIRCAPCTQEKLDEC-PAISPDCKQVLREPGC 63 |
| Sus scrofa | Query 25 IHCPPCSEEKLARCRP-PVGCEELVREPGC 53 +|| || |+ |+ | | |+|| |||+|||| Sbjct 24 VHCEPCDEKALSMCPPSPLGC-ELVKEPGC 52 |
| Pseudomonas syringae pv. tomato str. DC3000 | Query 20 LGDE#AIHCPPCSEEKLARCRPPVGCEELVREPGC 53 || |#|++ | | + +| || + |||+ + || Sbjct 26 LGSE#ALNQPFCFDIELVSARPDLKLEELLHKRGC 59 |
| Paracoccus denitrificans PD1222 | Query 1 MLPLCLVAALLLAAGPGPSLGDE#AIHCPPCSEEKL 35 +| + || ||| | || ||| # + || +|++ Sbjct 16 LLFISLVIFLLLEASPGDPLGDV#PLTVPPEVKERM 50 |
| Papio anubis | Query 26 HCPPCSEEKLARCRP-PVGCEELVREPGC 53 | ||| |||| | | | | |+ | || Sbjct 29 QCAPCSAEKLALCPPVPASCSEVTRSAGC 57 |
| swine, serum, Peptide Partial, 16 aa | Query 22 DE#AIHCPPCSEEKLAR 37 ||#||| || ||||||| Sbjct 1 DE#AIHXPPPSEEKLAR 16 |
[Site 2] GVCMELAEIE107-AIQESLQPSD
Glu107 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Gly98 | Val99 | Cys100 | Met101 | Glu102 | Leu103 | Ala104 | Glu105 | Ile106 | Glu107 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala108 | Ile109 | Gln110 | Glu111 | Ser112 | Leu113 | Gln114 | Pro115 | Ser116 | Asp117 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| LRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRR |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | N/A | 127.00 | 11 | insulin-like growth factor binding protein-4 |
| 2 | synthetic construct | 127.00 | 7 | insulin-like growth factor binding protein 4 |
| 3 | Homo sapiens | 127.00 | 7 | insulin-like growth factor binding protein 4 prec |
| 4 | Canis familiaris | 127.00 | 2 | PREDICTED: similar to Insulin-like growth factor |
| 5 | Equus caballus | 126.00 | 2 | insulin-like growth factor binding protein-4 |
| 6 | Bos taurus | 124.00 | 6 | insulin-like growth factor binding protein 4 |
| 7 | Ovis aries | 121.00 | 3 | IBP4_SHEEP Insulin-like growth factor-binding pro |
| 8 | Mus musculus | 109.00 | 9 | insulin-like growth factor binding protein-4 |
| 9 | Rattus norvegicus | 109.00 | 3 | insulin-like growth factor binding protein 4 |
| 10 | Gallus gallus | 105.00 | 3 | insulin-like growth factor binding protein 4 |
| 11 | Tetraodon nigroviridis | 66.60 | 5 | unnamed protein product |
| 12 | Sus scrofa | 51.20 | 3 | insulin-like growth factor binding protein 4 |
| 13 | Xenopus tropicalis | 42.40 | 2 | insulin-like growth factor binding protein 5 |
| 14 | Xenopus laevis | 41.60 | 2 | insulin-like growth factor binding protein 5 |
| 15 | Danio rerio | 38.50 | 5 | PREDICTED: similar to insulin-like growth factor |
| 16 | Oncorhynchus mykiss | 38.10 | 2 | insulin-like growth factor binding protein 5 |
| 17 | Coturnix japonica | 37.00 | 1 | AF293839_1 insulin-like growth factor binding pro |
| 18 | Bubalus bubalis | 37.00 | 1 | insulin-like growth factor binding protein-5 |
| 19 | Wolbachia endosymbiont of Drosophila simulans | 30.80 | 1 | DNA-directed RNA polymerase, beta/beta'' subunits |
| 20 | Wolbachia endosymbiont of Drosophila melanogaster | 30.80 | 1 | DNA-directed RNA polymerase, beta/beta' subunits |
Top-ranked sequences
| organism | matching |
|---|---|
| N/A | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 |
| synthetic construct | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 |
| Homo sapiens | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 |
| Canis familiaris | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||||||||||||||||||+|||#|||||||||||||||||||||||||||||| Sbjct 271 LRCYPPRGVEKPLHTLMHGQGVCMELSEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 330 |
| Equus caballus | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||||||||||||||||||||||#||||+||||||||||||||||||||||||| Sbjct 57 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQENLQPSDKDEGDHPNNSFSPCSAHDRR 116 |
| Bos taurus | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||||||||+|||||||||||||#|||||||||||||||||||||||||||||+ Sbjct 78 LRCYPPRGVEKPLHTLVHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRK 137 |
| Ovis aries | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 |||+||||||||||||+|||||||||||||#|||||||||||||||||||||||||||||+ Sbjct 57 LRCHPPRGVEKPLHTLVHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRK 116 |
| Mus musculus | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 +|||||||||||| ||||||||| ||+|||#||||||| ||||| +||||||+|||||| | Sbjct 78 MRCYPPRGVEKPLRTLMHGQGVCTELSEIE#AIQESLQTSDKDESEHPNNSFNPCSAHDHR 137 |
| Rattus norvegicus | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 +|||||||||||| ||||||||| ||+|||#||||||| ||||| +||||||+|||||| | Sbjct 78 MRCYPPRGVEKPLRTLMHGQGVCTELSEIE#AIQESLQTSDKDESEHPNNSFNPCSAHDHR 137 |
| Gallus gallus | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||| +|||||||||||| +||++|#|||||||| +|+| +|||||||||| |||+ Sbjct 83 LRCYPPRGVPRPLHTLMHGQGVCTDLADVE#AIQESLQPPEKEEIEHPNNSFSPCSIHDRK 142 |
| Tetraodon nigroviridis | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNSFSPCSAHDRR 137 ||||||||||+|||+|||||||| + |+|# + |+ +||||| || |+| Sbjct 87 LRCYPPRGVERPLHSLMHGQGVCTDEREVE#E-NSATDRQDEIIPEHPNNSNIRCSQQDKR 145 |
| Sus scrofa | Query 92 TLMHGQGVCMELAEIE#AIQESLQPS 116 |||||||+||||||||#||||||||| Sbjct 1 TLMHGQGLCMELAEIE#AIQESLQPS 25 |
| Xenopus tropicalis | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMEL------AEIE#AIQE 111 ||| | +| ||||| |+||+|||+ | ++|+#+|+| Sbjct 78 LRCLPEQGEEKPLHALLHGRGVCLNLKNHRDQSKID#SIEE 117 |
| Xenopus laevis | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMEL 103 ||| | +| ||||| |+||+|||+ | Sbjct 79 LRCLPEQGEEKPLHALLHGRGVCLNL 104 |
| Danio rerio | Query 78 LRCYPPRGVEKPLHTLMHGQGVCM 101 ||| | | ||||| |++|+|||| Sbjct 76 LRCLPRIGEEKPLHALLYGKGVCM 99 |
| Oncorhynchus mykiss | Query 78 LRCYPPRGVEKPLHTLMHGQGVC 100 ||| | | ||||| |+||+||| Sbjct 76 LRCLPRNGEEKPLHALLHGRGVC 98 |
| Coturnix japonica | Query 78 LRCYPPRGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPNNS 127 +|| | +| ||||| |+|| || |+| # +++ + | + | | Sbjct 34 VRCLPRQGEEKPLHALLHGTAVC--LSEKS#YREQAKAERESREHEEPTTS 81 |
| Bubalus bubalis | Query 78 LRCYPPRGVEKPLHTLMHGQGVCM 101 ||| | + ||||| |+||+|||+ Sbjct 34 LRCLPRQDEEKPLHALLHGRGVCL 57 |
| Wolbachia endosymbiont of Drosophila simulans | Query 84 RGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPN 125 | ||| ||| | + #|++ |||| || | | | Sbjct 808 RKVEKLHEGYDLPQGVSMSVKVFI#AVKHSLQPGDKMAGRHGN 849 |
| Wolbachia endosymbiont of Drosophila melanogaster | Query 84 RGVEKPLHTLMHGQGVCMELAEIE#AIQESLQPSDKDEGDHPN 125 | ||| ||| | + #|++ |||| || | | | Sbjct 1052 RKVEKLHEGYDLPQGVSMSVKVFI#AVKHSLQPGDKMAGRHGN 1093 |
[Site 3] HDRRCLQKHF143-AKIRDRSTSG
Phe143 Ala
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| His134 | Asp135 | Arg136 | Arg137 | Cys138 | Leu139 | Gln140 | Lys141 | His142 | Phe143 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Ala144 | Lys145 | Ile146 | Arg147 | Asp148 | Arg149 | Ser150 | Thr151 | Ser152 | Gly153 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| QPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGS |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | synthetic construct | 125.00 | 2 | insulin-like growth factor binding protein 4 |
| 2 | N/A | 125.00 | 2 | insulin-like growth factor binding protein-4 |
| 3 | Homo sapiens | 125.00 | 1 | insulin-like growth factor binding protein 4 prec |
| 4 | Equus caballus | 121.00 | 1 | insulin-like growth factor binding protein-4 |
| 5 | Canis familiaris | 120.00 | 1 | PREDICTED: similar to Insulin-like growth factor |
| 6 | Bos taurus | 118.00 | 2 | insulin-like growth factor binding protein 4 |
| 7 | Ovis aries | 116.00 | 1 | IBP4_SHEEP Insulin-like growth factor-binding pro |
| 8 | Mus musculus | 91.70 | 3 | insulin-like growth factor binding protein-4 |
| 9 | Rattus norvegicus | 90.90 | 1 | insulin-like growth factor binding protein 4 |
| 10 | Bubalus bubalis | 70.10 | 1 | insulin-like growth factor binding protein-4 |
| 11 | Gallus gallus | 69.70 | 1 | insulin-like growth factor binding protein 4 |
| 12 | Tetraodon nigroviridis | 33.50 | 1 | unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|---|
| synthetic construct | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 |
| N/A | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 |
| Homo sapiens | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 |
| Equus caballus | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 ||||||||||||||||||||||||||||||#||||||||||||||| | |||+|||||||| Sbjct 93 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVVGVPREEARPVPQGS 152 |
| Canis familiaris | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 ||||||||||||||||||||||||||||||#||||||||||||||| | |||+|||||||| Sbjct 307 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVIGVPREEARPVPQGS 366 |
| Bos taurus | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 |||||||||||||||||||||||+||||| #||||||||||||||| |||||+|||||||| Sbjct 114 QPSDKDEGDHPNNSFSPCSAHDRKCLQKHL#AKIRDRSTSGGKMKVIGAPREEARPVPQGS 173 |
| Ovis aries | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 |||||||||||||||||||||||+||||| #||||||||||||||| |||||+ ||||||| Sbjct 93 QPSDKDEGDHPNNSFSPCSAHDRKCLQKHL#AKIRDRSTSGGKMKVIGAPREEVRPVPQGS 152 |
| Mus musculus | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 | ||||| +||||||+|||||| |||||| #||||||| |||+ | |||+ ||||||| Sbjct 114 QTSDKDESEHPNNSFNPCSAHDHRCLQKHM#AKIRDRS----KMKIVGTPREEPRPVPQGS 169 |
| Rattus norvegicus | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 | ||||| +||||||+|||||| |||||| #||+|||| |||| | |||+ ||||||| Sbjct 114 QTSDKDESEHPNNSFNPCSAHDHRCLQKHM#AKVRDRS----KMKVVGTPREEPRPVPQGS 169 |
| Bubalus bubalis | Query 138 CLQKHF#AKIRDRSTSGGKMKVNGAPREDARPVPQGS 173 ||||| #||||||||||||||| |||||+|||||||| Sbjct 1 CLQKHL#AKIRDRSTSGGKMKVIGAPREEARPVPQGS 36 |
| Gallus gallus | Query 114 QPSDKDEGDHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTSGGKMKVNGAP--REDARPVPQ 171 || +|+| +|||||||||| |||+|||| #|| | +| ||+ +|+| ||++||+ | Sbjct 119 QPPEKEEIEHPNNSFSPCSIHDRKCLQKQQ#AK---RINNGNKMRSSGSPHHREESRPIAQ 175 Query 172 GS 173 || Query 172 GS 173 |
| Tetraodon nigroviridis | Query 122 DHPNNSFSPCSAHDRRCLQKHF#AKIRDRSTS 152 +||||| || |+||+|| #|+ +||+ Sbjct 130 EHPNNSNIRCSQQDKRCIQKTL#ARHLSKSTN 160 |
[Site 4] STSGGKMKVN159-GAPREDARPV
Asn159 Gly
|
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Ser150 | Thr151 | Ser152 | Gly153 | Gly154 | Lys155 | Met156 | Lys157 | Val158 | Asn159 |
|
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gly160 | Ala161 | Pro162 | Arg163 | Glu164 | Asp165 | Ala166 | Arg167 | Pro168 | Val169 |
Sequence conservation (by blast) Sequence conservation (by blast)
| Reference peptide (cleaved bond±30 residues) |
|---|
| PCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQ |
Summary
| # | organism | max score | hits | top seq |
|---|---|---|---|---|
| 1 | synthetic construct | 122.00 | 2 | insulin-like growth factor binding protein 4 |
| 2 | N/A | 122.00 | 2 | insulin-like growth factor binding protein-4 |
| 3 | Homo sapiens | 122.00 | 1 | insulin-like growth factor binding protein 4 prec |
| 4 | Canis familiaris | 117.00 | 1 | PREDICTED: similar to Insulin-like growth factor |
| 5 | Equus caballus | 116.00 | 1 | insulin-like growth factor binding protein-4 |
| 6 | Bos taurus | 114.00 | 2 | insulin-like growth factor binding protein 4 |
| 7 | Ovis aries | 112.00 | 1 | IBP4_SHEEP Insulin-like growth factor-binding pro |
| 8 | Bubalus bubalis | 99.00 | 1 | insulin-like growth factor binding protein-4 |
| 9 | Mus musculus | 96.30 | 3 | insulin-like growth factor binding protein-4 |
| 10 | Rattus norvegicus | 95.90 | 1 | insulin-like growth factor binding protein 4 |
| 11 | Gallus gallus | 75.50 | 1 | insulin-like growth factor binding protein 4 |
| 12 | Tetraodon nigroviridis | 35.80 | 1 | unnamed protein product |
Top-ranked sequences
| organism | matching |
|---|---|
| synthetic construct | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |
| N/A | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |
| Homo sapiens | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 ||||||||||||||||||||||||||||||#|||||||||||||||||||||||||||||| Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |
| Canis familiaris | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 ||||||||||||||||||||||||||||| #| |||+|||||||||||||||||||||||| Sbjct 323 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVI#GVPREEARPVPQGSCQSELHRALERLAASQ 382 |
| Equus caballus | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 ||||||||||||||||||||||||||||| #| |||+|||||||||||||||||||||||| Sbjct 109 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVV#GVPREEARPVPQGSCQSELHRALERLAASQ 168 |
| Bos taurus | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |||||||+||||| ||||||||||||||| #|||||+|||||||||||||||||||||||| Sbjct 130 PCSAHDRKCLQKHLAKIRDRSTSGGKMKVI#GAPREEARPVPQGSCQSELHRALERLAASQ 189 |
| Ovis aries | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |||||||+||||| ||||||||||||||| #|||||+ ||||||||||||||||||||||| Sbjct 109 PCSAHDRKCLQKHLAKIRDRSTSGGKMKVI#GAPREEVRPVPQGSCQSELHRALERLAASQ 168 |
| Bubalus bubalis | Query 138 CLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 ||||| ||||||||||||||| #|||||+|||||||||||||||||||||||| Sbjct 1 CLQKHLAKIRDRSTSGGKMKVI#GAPREEARPVPQGSCQSELHRALERLAASQ 52 |
| Mus musculus | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |||||| |||||| ||||||| |||+ #| |||+ ||||||||||||||||||||||| Sbjct 130 PCSAHDHRCLQKHMAKIRDRS----KMKIV#GTPREEPRPVPQGSCQSELHRALERLAASQ 185 |
| Rattus norvegicus | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAASQ 189 |||||| |||||| ||+|||| |||| #| |||+ ||||||||||||||||||||||| Sbjct 130 PCSAHDHRCLQKHMAKVRDRS----KMKVV#GTPREEPRPVPQGSCQSELHRALERLAASQ 185 |
| Gallus gallus | Query 130 PCSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAP--REDARPVPQGSCQSELHRALERLAA 187 ||| |||+|||| || | +| ||+ +#|+| ||++||+ ||||||||||||||||| Sbjct 135 PCSIHDRKCLQKQQAK---RINNGNKMRSS#GSPHHREESRPIAQGSCQSELHRALERLAA 191 Query 188 SQ 189 || Query 188 SQ 189 |
| Tetraodon nigroviridis | Query 131 CSAHDRRCLQKHFAKIRDRSTSGGKMKVN#GAPREDARPVPQGSCQSELHRALERLAAS 188 || |+||+|| |+ +||+ + +# + ||+ + | |++|| |||+|||++ Sbjct 139 CSQQDKRCIQKTLARHLSKSTN----QRS#NSGREETKAV-LAPCRAELQRALDRLASN 191 |
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