logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0074 : CAPN3, p94, calpain-3

[ CaMP Format ]

* Basic Information

OrganismOryctolagus cuniculus (rabbit)
Protein Namescalpain-3 [Oryctolagus cuniculus]; calpain-3; lens-specific calpain Lp82
Gene NamesCAPN3; calpain 3, (p94)
Gene Locus17; chromosome 17
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001075528 NM_001082059 100008726 Q9XSJ3 N/A N/A N/A ocu:100008726

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 709 aa

Average Mass: 82.559 kDa

Monoisotopic Mass: 82.507 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Calpain family cysteine protease 1. 513471.01.2
--- cleavage 274 (inside Calpain family cysteine protease 51..347) ---
--- cleavage 296 (inside Calpain family cysteine protease 51..347) ---
--- cleavage 322 (inside Calpain family cysteine protease 51..347) ---
Calpain large subunit, domain III 1. 3615101.00.1
EF-hand domain pair 1. 55660816.00.2
EF-hand domain pair 2. 61863231.00.0
EF-hand domain pair 3. 68469732.00.3

3. Sequence Information

Fasta Sequence: SB0074.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Not Available.

* Cleavage Information

3 [sites] cleaved by Calpain 3

Source Reference: [PubMed ID: 9794799] Kinbara K, Ishiura S, Tomioka S, Sorimachi H, Jeong SY, Amano S, Kawasaki H, Kolmerer B, Kimura S, Labeit S, Suzuki K, Purification of native p94, a muscle-specific calpain, and characterization of its autolysis. Biochem J. 1998 Nov 1;335 ( Pt 3):589-96.

Cleavage sites (±10aa)

[Site 1] GHAYSVTGLE274-ETLFKGEKVK

Glu274 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gly265His266Ala267Tyr268Ser269Val270Thr271Gly272Leu273Glu274
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Glu275Thr276Leu277Phe278Lys279Gly280Glu281Lys282Val283Lys284

Sequence conservation (by blast)

[Site 2] RLRNPWGQVE296-WNGSWSDGWK

Glu296 Trp

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Arg287Leu288Arg289Asn290Pro291Trp292Gly293Gln294Val295Glu296
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Trp297Asn298Gly299Ser300Trp301Ser302Asp303Gly304Trp305Lys306

Sequence conservation (by blast)

[Site 3] KDEKARLQHQ322-VTEDGEFWMS

Gln322 Val

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Lys313Asp314Glu315Lys316Ala317Arg318Leu319Gln320His321Gln322
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Val323Thr324Glu325Asp326Gly327Glu328Phe329Trp330Met331Ser332

Sequence conservation (by blast)

* References

None.