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SB0079 : Integrin [beta]1 isoform 1D

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesintegrin beta-1 isoform 1D precursor [Homo sapiens]; integrin beta-1 isoform 1D precursor; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; glycoprotein IIa; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta
Gene NamesITGB1; FNRB, MDF2, MSK12; FNRB; MDF2; MSK12; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Gene Locus10p11.2; chromosome 10
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_391988 NM_033668 3688 P05556 135630 HGNC:6153 00628 hsa:3688

* Information From OMIM

Function: Arregui et al. (2000) demonstrated that cell-permeable (Trojan) peptides containing the third helix of the antennapedia homeodomain fused to a peptide mimicking the juxtamembrane (JMP) region of the cytoplasmic domain of N-cadherin (CDH2; OMIM:114020) result in the inhibition of both CDH2 and ITGB1 function. Microscopic analysis showed that expression of JMP, which binds to the cytoplasmic domain of CDH2, results in a reduction of neurite outgrowth on cadherin substrates. Treatment of cells with JMP resulted in the release of FER (OMIM:176942) from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1.

* Structure Information

1. Primary Information

Length: 801 aa

Average Mass: 88.883 kDa

Monoisotopic Mass: 88.825 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Integrin beta tail domain 1. 33355.02.2
EGF-like domain 1. 363912.01.7
Integrin beta tail domain 2. 20722148.00.0
Integrin beta tail domain 3. 41344225.01.4
EGF-like domain 2. 4765007.018.7
EGF-like domain 3. 5165531.013.5
Integrin beta tail domain 4. 57959813.014.8
EGF-like domain 4. 5996301.016.5
EGF-like domain 5. 63864112.05.8
Integrin beta cytoplasmic domain 1. 7527961.02.3
--- cleavage 767 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 771 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 772 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 777 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 778 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 783 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 788 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 789 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 790 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 791 (inside Integrin beta cytoplasmic domain 752..796) ---
--- cleavage 795 (inside Integrin beta cytoplasmic domain 752..796) ---

3. Sequence Information

Fasta Sequence: SB0079.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1K11 (Model; -; B=786-797), 1LHA (Model; -; A=86-543), 3G9W (X-ray; 216 A; C/D=752-785), 3T9K (X-ray; 230 A; A/B=758-769), 3VI3 (X-ray; 290 A; B/D=21-465), 3VI4 (X-ray; 290 A; B/D=21-465), 4DX9 (X-ray; 300 A; 6/7/8/9/B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f/h/j/l/n/p/r/t/v/x/z=784-798), 4WJK (X-ray; 185 A; B=21-465), 4WK0 (X-ray; 178 A; B=21-465), 4WK2 (X-ray; 250 A; B=21-465), 4WK4 (X-ray; 250 A; B=21-465)

* Cleavage Information

11 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 10571053] Pfaff M, Du X, Ginsberg MH, Calpain cleavage of integrin beta cytoplasmic domains. FEBS Lett. 1999 Oct 22;460(1):17-22.

Cleavage sites (±10aa)

[Site 1] HDRREFAKFE767-KEKMNAKWDT

Glu767 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
His758Asp759Arg760Arg761Glu762Phe763Ala764Lys765Phe766Glu767
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys768Glu769Lys770Met771Asn772Ala773Lys774Trp775Asp776Thr777

Sequence conservation (by blast)

[Site 2] EFAKFEKEKM771-NAKWDTQENP

Met771 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Glu762Phe763Ala764Lys765Phe766Glu767Lys768Glu769Lys770Met771
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asn772Ala773Lys774Trp775Asp776Thr777Gln778Glu779Asn780Pro781

Sequence conservation (by blast)

[Site 3] FAKFEKEKMN772-AKWDTQENPI

Asn772 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Phe763Ala764Lys765Phe766Glu767Lys768Glu769Lys770Met771Asn772
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala773Lys774Trp775Asp776Thr777Gln778Glu779Asn780Pro781Ile782

Sequence conservation (by blast)

[Site 4] KEKMNAKWDT777-QENPIYKSPI

Thr777 Gln

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Lys768Glu769Lys770Met771Asn772Ala773Lys774Trp775Asp776Thr777
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gln778Glu779Asn780Pro781Ile782Tyr783Lys784Ser785Pro786Ile787

Sequence conservation (by blast)

[Site 5] EKMNAKWDTQ778-ENPIYKSPIN

Gln778 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Glu769Lys770Met771Asn772Ala773Lys774Trp775Asp776Thr777Gln778
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Glu779Asn780Pro781Ile782Tyr783Lys784Ser785Pro786Ile787Asn788

Sequence conservation (by blast)

[Site 6] KWDTQENPIY783-KSPINNFKNP

Tyr783 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Lys774Trp775Asp776Thr777Gln778Glu779Asn780Pro781Ile782Tyr783
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys784Ser785Pro786Ile787Asn788Asn789Phe790Lys791Asn792Pro793

Sequence conservation (by blast)

[Site 7] ENPIYKSPIN788-NFKNPNYGRK

Asn788 Asn

iTraq-117 Signal 9143.1 () for NFKNPNYGRK

iTraq-117 Signal 2019.9 () for FKNPNYGRK

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Glu779Asn780Pro781Ile782Tyr783Lys784Ser785Pro786Ile787Asn788
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asn789Phe790Lys791Asn792Pro793Asn794Tyr795Gly796Arg797Lys798

Sequence conservation (by blast)

[Site 8] NPIYKSPINN789-FKNPNYGRKA

Asn789 Phe

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Asn780Pro781Ile782Tyr783Lys784Ser785Pro786Ile787Asn788Asn789
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Phe790Lys791Asn792Pro793Asn794Tyr795Gly796Arg797Lys798Ala799

Sequence conservation (by blast)

[Site 9] PIYKSPINNF790-KNPNYGRKAG

Phe790 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Pro781Ile782Tyr783Lys784Ser785Pro786Ile787Asn788Asn789Phe790
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys791Asn792Pro793Asn794Tyr795Gly796Arg797Lys798Ala799Gly800

Sequence conservation (by blast)

[Site 10] IYKSPINNFK791-NPNYGRKAGL

Lys791 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ile782Tyr783Lys784Ser785Pro786Ile787Asn788Asn789Phe790Lys791
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asn792Pro793Asn794Tyr795Gly796Arg797Lys798Ala799Gly800Leu801

Sequence conservation (by blast)

[Site 11] PINNFKNPNY795-GRKAGL

Tyr795 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Pro786Ile787Asn788Asn789Phe790Lys791Asn792Pro793Asn794Tyr795
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly796Arg797Lys798Ala799Gly800Leu801----

Sequence conservation (by blast)

* References

[PubMed ID: 24510996] Lee C, Lee C, Lee S, Siu A, Ramos DM, The cytoplasmic extension of the integrin beta6 subunit regulates epithelial-to-mesenchymal transition. Anticancer Res. 2014 Feb;34(2):659-64.

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