SB0081 : Talin 1
[ CaMP Format ]
* Basic Information
| Organism | Mus musculus (house mouse) |
| Protein Names | talin 1 [Mus musculus]; talin 1; Talin-1 |
| Gene Names | Tln1; Tln; talin 1 |
| Gene Locus | 4; chromosome 4 |
| GO Function | Not available |
* Information From OMIM
Not Available.
* Structure Information
1. Primary Information
Length: 2541 aa
Average Mass: 269.830 kDa
Monoisotopic Mass: 269.665 kDa
2. Domain Information
Annotated Domains: interpro / pfam / smart / prosite
Computationally Assigned Domains (Pfam+HMMER):
| domain name | begin | end | score | e-value |
|---|---|---|---|---|
| FERM N-terminal domain 1. | 46 | 69 | 37.0 | 0.0 |
| Ras association (RalGDS/AF-6) domain 1. | 89 | 158 | 6.0 | 0.1 |
| N-terminal or F0 domain of Talin-head FERM 1. | 172 | 188 | 59.0 | 0.0 |
| Protein of unknown function (DUF1013) 1. | 194 | 216 | 104.0 | 0.0 |
| I/LWEQ domain 1. | 287 | 306 | 116.0 | 0.1 |
| PTB domain (IRS-1 type) 1. | 328 | 397 | 24.0 | 0.0 |
| --- cleavage 433 --- | ||||
| I/LWEQ domain 2. | 488 | 547 | 74.0 | 0.1 |
| Talin, middle domain 1. | 664 | 764 | 50.0 | 8.4 |
| Talin, middle domain 2. | 842 | 895 | 109.0 | 7.1 |
| I/LWEQ domain 3. | 904 | 976 | 28.0 | 6.2 |
| Talin, middle domain 3. | 1006 | 1042 | 109.0 | 4.3 |
| I/LWEQ domain 4. | 1084 | 1114 | 67.0 | 0.0 |
| PMC2NT (NUC016) domain 1. | 1178 | 1203 | 22.0 | 0.1 |
| Talin, middle domain 4. | 1238 | 1276 | 112.0 | 5.8 |
| I/LWEQ domain 5. | 1294 | 1362 | 35.0 | 2.6 |
| Talin, middle domain 5. | 1490 | 1545 | 82.0 | 0.9 |
| Vinculin Binding Site 1. | 1609 | 1649 | 78.0 | 0.0 |
| Talin, middle domain 6. | 1755 | 1789 | 111.0 | 5.6 |
| Talin, middle domain 7. | 1935 | 1985 | 105.0 | 0.3 |
| Talin, middle domain 8. | 2010 | 2102 | 44.0 | 5.5 |
| I/LWEQ domain 6. | 2185 | 2220 | 27.0 | 0.2 |
| Talin, middle domain 9. | 2266 | 2322 | 113.0 | 0.1 |
| Talin, middle domain 10. | 2341 | 2367 | 112.0 | 3.3 |
| Talin, middle domain 11. | 2382 | 2439 | 105.0 | 2.8 |
| PMC2NT (NUC016) domain 2. | 2450 | 2476 | 23.0 | 0.1 |
3. Sequence Information
Fasta Sequence: SB0081.fasta
Amino Acid Sequence and Secondary Structures (PsiPred):
4. 3D Information
Known Structures in PDB: 1SJ7 (X-ray; 250 A; A/B/C=482-655), 1SJ8 (X-ray; 260 A; A=482-789), 1T01 (X-ray; 206 A; B=605-628), 1U89 (NMR; -; A=755-889), 1XWX (Model; -; A=486-889), 1Y19 (X-ray; 260 A; B/D/F/H/J/L=209-410), 1ZW3 (X-ray; 330 A; B=1628-1652), 2B0H (NMR; -; A=1843-1973), 2G35 (NMR; -; A=305-404), 2JSW (NMR; -; A=2300-2482), 2KBB (NMR; -; A=1655-1822), 2KC1 (NMR; -; A=1-85), 2KC2 (NMR; -; A=86-202), 2KGX (NMR; -; A=1655-1822, B=311-401), 2KMA (NMR; -; A=1-202), 2KVP (NMR; -; A=1815-1973), 2L10 (NMR; -; A=1206-1357), 2L7A (NMR; -; A=787-911), 2L7N (NMR; -; A=1046-1207), 2LQG (NMR; -; A=913-1044), 2QDQ (X-ray; 220 A; A/B=2494-2541), 2X0C (X-ray; 200 A; A=1359-1659), 3DYJ (X-ray; 185 A; A/B=1974-2293), 3IVF (X-ray; 194 A; A=1-400), 3S90 (X-ray; 197 A; C/D=1512-1546), 4F7G (X-ray; 205 A; A=206-405, B=1654-1847), 4W8P (X-ray; 150 A; A=1357-1657)
* Cleavage Information
1 [sites] cleaved by Calpain 1
Source Reference: [PubMed ID: 2120593] Rees DJ, Ades SE, Singer SJ, Hynes RO, Sequence and domain structure of talin. Nature. 1990 Oct 18;347(6294):685-9.
Cleavage sites (±10aa)
[Site 1] VSPKKSTVLQ433-QQYNRVGKVE
Gln433 
Gln
iTraq-117 Signal 7123.5 (
) for QQYNRVGKVE
 |
|||||||||
| P10 | P9 | P8 | P7 | P6 | P5 | P4 | P3 | P2 | P1 |
|---|---|---|---|---|---|---|---|---|---|
| Val424 | Ser425 | Pro426 | Lys427 | Lys428 | Ser429 | Thr430 | Val431 | Leu432 | Gln433 |
 |
|||||||||
| P1' | P2' | P3' | P4' | P5' | P6' | P7' | P8' | P9' | P10' |
| Gln434 | Gln435 | Tyr436 | Asn437 | Arg438 | Val439 | Gly440 | Lys441 | Val442 | Glu443 |
Sequence conservation (by blast)
* References
[PubMed ID: 19124737] Mace EM, Monkley SJ, Critchley DR, Takei F, A dual role for talin in NK cell cytotoxicity: activation of LFA-1-mediated cell adhesion and polarization of NK cells. J Immunol. 2009 Jan 15;182(2):948-56.