CaMPDB :: Substrate

SB0082 : Crystallin, [beta]B2

* Basic Information

Organism	Bos taurus (cattle)
Protein Names	beta-crystallin B2 [Bos taurus]; beta-crystallin B2; beta-B2 crystallin; beta-crystallin Bp; Beta-crystallin B2; Beta-B2 crystallin; Beta-crystallin Bp
Gene Names	CRYBB2; crystallin, beta B2
Gene Locus	17; chromosome 17
GO Function	Not available

Entrez Protein	Entrez Nucleotide	Entrez Gene	UniProt	OMIM	HGNC	HPRD	KEGG
NP_777232	NM_174807	287011	P02522	N/A	N/A	N/A	bta:287011

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 205 aa

Average Mass: 23.298 kDa

Monoisotopic Mass: 23.283 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain name	begin	end	score	e-value
--- cleavage 8 ---
Peptidase inhibitor family I36 1.	21	96	12.0	0.0
Peptidase inhibitor family I36 2.	108	191	9.0	0.0

3. Sequence Information

Fasta Sequence: SB0082.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1BLB (X-ray; 330 A; A/B/C/D=2-205), 2BB2 (X-ray; 210 A; A=15-195)

* Cleavage Information

1 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 9485487] Shih M, Lampi KJ, Shearer TR, David LL, Cleavage of beta crystallins during maturation of bovine lens. Mol Vis. 1998 Feb 27;4:4.

Cleavage sites (±10aa)

[Site 1] MASDHQTQ⁸-AGKPQPLNPK

Gln⁸ Ala

P10	P9	P8	P7	P6	P5	P4	P3	P2	P1

-	-	Met¹	Ala²	Ser³	Asp⁴	His⁵	Gln⁶	Thr⁷	Gln⁸

P1'	P2'	P3'	P4'	P5'	P6'	P7'	P8'	P9'	P10'
Ala⁹	Gly¹⁰	Lys¹¹	Pro¹²	Gln¹³	Pro¹⁴	Leu¹⁵	Asn¹⁶	Pro¹⁷	Lys¹⁸

Sequence conservation (by blast) Sequence conservation (by blast)

Reference peptide (cleaved bond±30 residues)
MASDHQTQAGKPQPLNPKIIIFEQENFQGHSHELNGPC

Summary

#	organism	max score	hits	top seq
1	Bos taurus	84.30	2	crystallin, beta B2
2	Mus musculus	82.40	7	crystallin, beta B2
3	Canis lupus familiaris	82.00	1	crystallin, beta B2
4	Homo sapiens	81.30	4	crystallin, beta B2
5	N/A	79.30	7	A Chain A, The N-Terminal Domain Of Beta-B2-Crysta
6	human, lens, Peptide, 204 aa	79.00	1	A Chain A, Structure Of Native Human Beta B2 Crys
7	Rana catesbeiana	54.70	4	CRBB2_RANCA Beta-crystallin B2 (Beta-B2 crystalli
8	Gallus gallus	51.60	3	CRBB2_CHICK Beta-crystallin B2 (Beta-B2 crystallin
9	Danio rerio	45.10	11	PREDICTED: beta B2-crystallin isoform 1
10	Tetraodon nigroviridis	41.20	10	unnamed protein product
11	Canis familiaris	37.40	1	PREDICTED: similar to crystallin, beta B1
12	Xenopus laevis	33.90	2	Unknown (protein for MGC:115166)
13	Xenopus tropicalis	33.90	1	crystallin, beta B1
14	Rattus norvegicus	32.70	1	AF286652_1 betaB1-crystallin
15	Rattus norvegicus	32.30	3	unnamed protein product
16	Pan troglodytes	32.30	1	PREDICTED: crystallin, beta B1 isoform 2
17	Rana temporaria	32.00	2	unnamed protein product
18	Cynops pyrrhogaster	32.00	1	newt beta B1-crystallin
19	synthetic construct	32.00	1	crystallin beta B1
20	Dicentrarchus labrax	30.80	1	crystallin beta B1
21	Petenia splendida x Cichlasoma synspilum	30.80	1	AAA50959.1
22	Cavia porcellus	30.00	2	beta A1-crystallin
23	Danio rerio	30.00	1	crystallin, gamma M2d6
24	Oryctolagus cuniculus	29.60	1	beta A1-crystallin
25	Oryctolagus cuniculus	29.60	1	beta A3-crystallin
26	Cyprinus carpio	29.30	1	CRGM3_CYPCA Gamma-crystallin M3 (Gamma-M3) gi

Top-ranked sequences

organism	matching
Bos taurus	Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38
Mus musculus	Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELSGPC 38
Canis lupus familiaris	Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELSGPC 38
Homo sapiens	Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 MASDHQTQ#AGKPQSLNPKIIIFEQENFQGHSHELNGPC 38
N/A	Query 2 ASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|\|#\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|+\|\|\| Sbjct 1 ASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELSGPC 37
human, lens, Peptide, 204 aa	Query 2 ASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|\|#\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Sbjct 1 ASDHQTQ#AGKPQSLNPKIIIFEQENFQGHSHELNGPC 37
Rana catesbeiana	Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|\|\|\|+ #\| \| \| + \|\|++\|\|\|\|\|\|\|\| \|\|\|+\|\|\| Sbjct 1 MASDHQSP#ATKQQQPSSKIVLFEQENFQGRCHELSGPC 38
Gallus gallus	Query 1 MASDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|\|\|+\|\| #\| \| \|\| +\| \| \|\|\|\|\|\|\|\|\| \|\|\|+\| \| Sbjct 1 MASEHQMP#ASKQQPASPNIAIFEQENFQGRCHELSGAC 38
Danio rerio	Query 1 MASDHQTQ#AGK-PQPLNP--KIIIFEQENFQGHSHELNGPC 38 \|\|+\|\|\| #\| \| \|\|+ \|++\|+\|\|\|\|\|\|\| \|\|\| \|\|\| Sbjct 1 MATDHQNP#ATKQKQPVASAFKLVIYEQENFQGRCHELTGPC 41
Tetraodon nigroviridis	Query 1 MASDHQTQ#AGKPQPLNP---KIIIFEQENFQGHSHELNGPC 38 \|\|+\|\|\| #\| \| \| + \|++\|+\|\|\|\|\|\|\|\| \|\|+ \| Sbjct 25 MATDHQNP#ASKQQQPSASAFKLVIYEQENFQGHCQELSAAC 65
Canis familiaris	Query 7 TQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|+#\|\| \| + \|+++\|\|\|\|\|\|\|\| \| +\| \| Sbjct 45 TK#AGDLPPGSYKLVVFEQENFQGRRVEFSGEC 76
Xenopus laevis	Query 7 TQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|+# \|+\| \|\|++\|\|\|\|\|\|\|\| \|\| \| Sbjct 27 TR#TGEPLNGTFKIVLFEQENFQGRHMELLNEC 58
Xenopus tropicalis	Query 3 SDHQTQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \| \|+# \| \| + \|\|++\|\|\|\|\|\|\|\| \|\| \| Sbjct 23 SAKPTR#TGDPMMGSFKIVLFEQENFQGRHVELLNEC 58
Rattus norvegicus	Query 8 Q#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 +# \|+ \| + ++++\|\|\|\|\|\|\|\| \| +\| \| Sbjct 43 K#VGELPPGSYRLVVFEQENFQGRRVEFSGEC 73
Rattus norvegicus	Query 14 PLNPKIIIFEQENFQGHSHELNGPC 38 \| + ++++\|\|\|\|\|\|\|\| \| +\| \| Sbjct 3 PGSYRLVVFEQENFQGRRVEFSGEC 27
Pan troglodytes	Query 8 Q#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 +# \|+ \| \| ++++\|\| \|\|\|\|\| \| +\| \| Sbjct 50 K#VGELPPGNYRLVVFELENFQGRRAEFSGEC 80
Rana temporaria	Query 12 PQPLNP-KIIIFEQENFQGHSHELNGPC 38 \| \|\| \| \|\| +++\|\|\|\|\|\| \| \| Sbjct 8 PVPLGPWKITVYDQENFQGKRMEFTSSC 35
Cynops pyrrhogaster	Query 8 Q#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 +#\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\| \| Sbjct 39 K#AGDSSVGTYKIIIFEQENFQGRHLELVNEC 69
synthetic construct	Query 8 Q#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 +#\| + \| \| ++++\|\| \|\|\|\|\| \| +\| \| Sbjct 50 K#AAELPPGNYRLVVFELENFQGRRAEFSGEC 80
Dicentrarchus labrax	Query 7 TQ#AGKPQPLNPKIIIFEQENFQGHSHELNGPC 38 \|+# \|+\| + +\|++\|+\|\|\|\|\|\| \|+ \| Sbjct 30 TK#TGEPGMGSYRIMMFDQENFQGRMIEVQNEC 61
Petenia splendida x Cichlasoma synspilum	Query 18 KIIIFEQENFQGHSHELNGPC 38 ++ \|+\|+\|\|\|\|\| +\|\|\| \| Sbjct 89 RMRIYERENFQGQNHELMNDC 109
Cavia porcellus	Query 12 PQPLNP-KIIIFEQENFQGHSHELNGPC 38 \| \| \| \|\| \|++\|\|\|\|\|\| \| \| Sbjct 8 PGSLGPWKITIYDQENFQGKRMEFTSSC 35
Danio rerio	Query 18 KIIIFEQENFQGHSHELNGPC 38 \|+\| +\| \|\|\|\| \|+\| \| \| Sbjct 3 KVIFYEDRNFQGRSYECMGDC 23
Oryctolagus cuniculus	Query 12 PQPLNP-KIIIFEQENFQGHSHELNGPC 38 \| \| \| \|\| \|++\|\|\|\|\|\| \| \| Sbjct 8 PGSLGPWKITIYDQENFQGKRMEFTSSC 35
Oryctolagus cuniculus	Query 12 PQPLNP-KIIIFEQENFQGHSHELNGPC 38 \| \| \| \|\| \|++\|\|\|\|\|\| \| \| Sbjct 25 PGSLGPWKITIYDQENFQGKRMEFTSSC 52
Cyprinus carpio	Query 18 KIIIFEQENFQGHSHELNGPC 38 \|\|\| +\| \|\|\|\| \|+\| + \| Sbjct 3 KIIFYEDRNFQGRSYECSSDC 23

* References

[PubMed ID: 19206113] Diemer H, Atmanene C, Sanglier S, Morrissey B, Van Dorsselaer A, Downard KM, Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS). J Mass Spectrom. 2009 May;44(5):803-12. doi: 10.1002/jms.1560.

» More (8) « Hide Below